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Protein

Centrosomal protein of 55 kDa

Gene

Cep55

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in mitotic exit and cytokinesis. Recruits PDCD6IP and TSG101 to midbody during cytokinesis. Required for successful completion of cytokinesis. Not required for microtubule nucleation. Plays a role in the development of the brain and kidney.By similarity

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, Mitosis

Names & Taxonomyi

Protein namesi
Recommended name:
Centrosomal protein of 55 kDaImported
Short name:
Cep55
Gene namesi
Name:Cep55Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1921357 Cep55

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002386651 – 462Centrosomal protein of 55 kDaAdd BLAST462

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei96PhosphoserineCombined sources1
Modified residuei99PhosphoserineBy similarity1
Modified residuei423PhosphoserineBy similarity1
Modified residuei426PhosphoserineCombined sources1
Modified residuei428PhosphoserineCombined sources1
Modified residuei434Phosphoserine; by PLK1Combined sources1

Post-translational modificationi

There is a hierachy of phosphorylation, where both Ser-423 and Ser-426 are phosphorylated at the onset of mitosis, prior to Ser-434. Phosphorylation at Ser-423 and Ser-426 is required for dissociation from the centrosome at the G2/M boundary. Phosphorylation at the 3 sites, Ser-423, Ser-426 and Ser-434, is required for protein function at the final stages of cell division to complete cytokinesis successfully (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8BT07
PaxDbiQ8BT07
PeptideAtlasiQ8BT07
PRIDEiQ8BT07

PTM databases

iPTMnetiQ8BT07
PhosphoSitePlusiQ8BT07

Expressioni

Gene expression databases

BgeeiENSMUSG00000024989 Expressed in 193 organ(s), highest expression level in embryo
CleanExiMM_CEP55
GenevisibleiQ8BT07 MM

Interactioni

Subunit structurei

Homodimer. Interacts (phosphorylated on Ser-423 and Ser-426) with PLK1. Interacts with AKAP9/CG-NAP; the interaction occurs in interphase and is lost upon mitotic entry. Interacts with PCNT/Kendrin; the interaction occurs in interphase and is lost upon mitotic entry. Directly interacts with PDCD6IP; this interaction is required for PDCD6IP targeting to the midbody; CEP55 binds PDCD6IP in a 2:1 stoichiometry; PDCD6IP competes with TSG101 for the same binding site. Interacts with TSG101; TSG101 competes with PDCD6IP for the same binding site; interaction is required for cytokinesis. Interacts with MVB12A, VPS37B, VPS37C and VPS28 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Tex14Q7M6U311EBI-2552328,EBI-6674575

Protein-protein interaction databases

BioGridi216498, 42 interactors
IntActiQ8BT07, 49 interactors
STRINGi10090.ENSMUSP00000093802

Structurei

3D structure databases

ProteinModelPortaliQ8BT07
SMRiQ8BT07
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni157 – 235Interaction with TSG101By similarityAdd BLAST79
Regioni160 – 214Interaction with PDCD6IPBy similarityAdd BLAST55
Regioni354 – 462Required for localization to the interphase centrosome and to the midbody during cytokinesisBy similarityAdd BLAST109

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili50 – 400Sequence analysisAdd BLAST351

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IFBZ Eukaryota
ENOG41120Y2 LUCA
GeneTreeiENSGT00510000047961
HOGENOMiHOG000111547
HOVERGENiHBG081092
InParanoidiQ8BT07
KOiK16456
OMAiYDQQREV
OrthoDBiEOG091G065Y
PhylomeDBiQ8BT07
TreeFamiTF331107

Family and domain databases

InterProiView protein in InterPro
IPR038926 CEP55
IPR022008 EABR
PANTHERiPTHR31838 PTHR31838, 1 hit
PfamiView protein in Pfam
PF12180 EABR, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q8BT07-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSSRSPKDLI KSKWGSRPSS SKSDTALEKF KGEIAAFKTS LDEITSGKGK
60 70 80 90 100
MAEKGRSRLL EKIQVLEAER EKNVYYLLEK DKEIQRLKDH LRSRYSSSSL
110 120 130 140 150
FEQLEEKTKE CEKKQQLLES LSKETDVLKN QLSATTKRLS ELESKASTLH
160 170 180 190 200
LSQSMPANCF NSSMNSIHEK EMQLKDALEK NQQWLVYDQQ REAYVKGLLA
210 220 230 240 250
KIFELEKRTE TAAASLTQQM KKIESEGYLQ VEKQKYDHLL ENAKKDLEVE
260 270 280 290 300
RQAVTQLRLE LDEFRRKYEE ARKEVEDLNQ LLSSQRKADI QHLEEDKQKT
310 320 330 340 350
ERIQKLREES SIFKGKLEEE RKRSEELLSQ VRILYDSLLK HQEEQARVAL
360 370 380 390 400
LEQQMQACTL DFENEKLDRQ NMQHQLYVIL KELRKAKSQI TQLESLKQLH
410 420 430 440 450
GFTITEQPFP LQREPESRVK ATSPKSPSAA LNDSLVECPK CSVQYPATEH
460
RDLLVHVEYC MK
Length:462
Mass (Da):53,930
Last modified:May 30, 2006 - v2
Checksum:i5D2308BFCEADA08B
GO
Isoform 2 (identifier: Q8BT07-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     397-397: Missing.

Note: No experimental confirmation available.
Show »
Length:461
Mass (Da):53,802
Checksum:iC0816F19989B4FA1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti70R → G in BAC40417 (PubMed:16141072).Curated1
Sequence conflicti106E → K in BAC40417 (PubMed:16141072).Curated1
Sequence conflicti157A → P in BAC25819 (PubMed:16141072).Curated1
Sequence conflicti253A → T in AAH26966 (PubMed:15489334).Curated1
Sequence conflicti259L → V in AAH26966 (PubMed:15489334).Curated1
Sequence conflicti300T → M in AAH26966 (PubMed:15489334).Curated1
Sequence conflicti406E → D in BAC25819 (PubMed:16141072).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti55G → D2 Publications1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_018630397Missing in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004655 mRNA Translation: BAB23446.1
AK028216 mRNA Translation: BAC25819.1
AK088548 mRNA Translation: BAC40417.1
BC026966 mRNA Translation: AAH26966.1
BC031396 mRNA Translation: AAH31396.1
CCDSiCCDS29782.1 [Q8BT07-2]
CCDS50429.1 [Q8BT07-1]
RefSeqiNP_001157834.1, NM_001164362.1 [Q8BT07-1]
NP_082569.1, NM_028293.1 [Q8BT07-1]
NP_083036.2, NM_028760.2 [Q8BT07-2]
XP_006527459.1, XM_006527396.2 [Q8BT07-1]
XP_006527460.1, XM_006527397.3 [Q8BT07-1]
XP_006527461.1, XM_006527398.3 [Q8BT07-1]
XP_006527462.1, XM_006527399.2 [Q8BT07-1]
XP_006527463.1, XM_006527400.2
XP_006527464.1, XM_006527401.2 [Q8BT07-1]
XP_011245683.1, XM_011247381.2 [Q8BT07-1]
UniGeneiMm.9916

Genome annotation databases

EnsembliENSMUST00000096096; ENSMUSP00000093802; ENSMUSG00000024989 [Q8BT07-1]
ENSMUST00000116506; ENSMUSP00000112205; ENSMUSG00000024989 [Q8BT07-2]
ENSMUST00000169673; ENSMUSP00000127961; ENSMUSG00000024989 [Q8BT07-1]
GeneIDi74107
KEGGimmu:74107
UCSCiuc008hiy.2 mouse [Q8BT07-1]
uc008hja.2 mouse [Q8BT07-2]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCEP55_MOUSE
AccessioniPrimary (citable) accession number: Q8BT07
Secondary accession number(s): Q8C2J0
, Q8K2I8, Q8R2Y4, Q9DBZ8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: May 30, 2006
Last modified: September 12, 2018
This is version 116 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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