UniProtKB - Q8BT07 (CEP55_MOUSE)
Centrosomal protein of 55 kDa
Cep55
Functioni
GO - Molecular functioni
- identical protein binding Source: MGI
GO - Biological processi
- cranial skeletal system development Source: UniProtKB
- establishment of protein localization Source: MGI
- midbody abscission Source: MGI
- mitotic cytokinesis Source: MGI
- regulation of phosphatidylinositol 3-kinase signaling Source: InterPro
Keywordsi
Biological process | Cell cycle, Cell division, Mitosis |
Names & Taxonomyi
Protein namesi | Recommended name: Centrosomal protein of 55 kDaImportedShort name: Cep55 |
Gene namesi | Name:Cep55Imported |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1921357, Cep55 |
Subcellular locationi
Cytoskeleton
- centriole By similarity
- centrosome By similarity
Other locations
- Cytoplasm By similarity
- Cleavage furrow By similarity
- Midbody ring By similarity
Note: Present at the centrosomes at interphase. A small portion is associated preferentially with the mother centriole, whereas the majority localizes to the pericentriolar material. During mitosis, loses affinity for the centrosome at the onset of prophase and diffuses throughout the cell. This dissociation from the centrosome is phosphorylation-dependent. May remain localized at the centrosome during mitosis in certain cell types. Appears at the cleavage furrow in late anaphase and in the midbody in cytokinesis.By similarity
Cytoskeleton
- centriolar satellite Source: MGI
- centriole Source: UniProtKB-SubCell
- centrosome Source: MGI
Extracellular region or secreted
- intercellular bridge Source: MGI
Plasma Membrane
- cleavage furrow Source: UniProtKB-SubCell
- plasma membrane Source: MGI
Other locations
- cytoplasm Source: UniProtKB-SubCell
- Flemming body Source: UniProtKB
- midbody Source: MGI
Keywords - Cellular componenti
Cytoplasm, CytoskeletonPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000238665 | 1 – 462 | Centrosomal protein of 55 kDaAdd BLAST | 462 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 96 | PhosphoserineCombined sources | 1 | |
Modified residuei | 99 | PhosphoserineBy similarity | 1 | |
Modified residuei | 423 | PhosphoserineBy similarity | 1 | |
Modified residuei | 426 | PhosphoserineCombined sources | 1 | |
Modified residuei | 428 | PhosphoserineCombined sources | 1 | |
Modified residuei | 434 | Phosphoserine; by PLK1Combined sources | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q8BT07 |
jPOSTi | Q8BT07 |
PaxDbi | Q8BT07 |
PeptideAtlasi | Q8BT07 |
PRIDEi | Q8BT07 |
PTM databases
iPTMneti | Q8BT07 |
PhosphoSitePlusi | Q8BT07 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000024989, Expressed in embryo and 212 other tissues |
Genevisiblei | Q8BT07, MM |
Interactioni
Subunit structurei
Homodimer.
Interacts (phosphorylated on Ser-423 and Ser-426) with PLK1.
Interacts with AKAP9/CG-NAP; the interaction occurs in interphase and is lost upon mitotic entry.
Interacts with PCNT/Kendrin; the interaction occurs in interphase and is lost upon mitotic entry. Directly interacts with PDCD6IP; this interaction is required for PDCD6IP targeting to the midbody; CEP55 binds PDCD6IP in a 2:1 stoichiometry; PDCD6IP competes with TSG101 for the same binding site.
Interacts with TSG101; TSG101 competes with PDCD6IP for the same binding site; interaction is required for cytokinesis.
Interacts with MVB12A, VPS37B, VPS37C and VPS28 (By similarity).
By similarityBinary interactionsi
Q8BT07
With | #Exp. | IntAct |
---|---|---|
Tex14 [Q7M6U3] | 11 | EBI-2552328,EBI-6674575 |
GO - Molecular functioni
- identical protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 216498, 42 interactors |
IntActi | Q8BT07, 49 interactors |
STRINGi | 10090.ENSMUSP00000127961 |
Miscellaneous databases
RNActi | Q8BT07, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 157 – 235 | Interaction with TSG101By similarityAdd BLAST | 79 | |
Regioni | 160 – 214 | Interaction with PDCD6IPBy similarityAdd BLAST | 55 | |
Regioni | 354 – 462 | Required for localization to the interphase centrosome and to the midbody during cytokinesisBy similarityAdd BLAST | 109 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 50 – 400 | Sequence analysisAdd BLAST | 351 |
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | ENOG502QTDI, Eukaryota |
GeneTreei | ENSGT00510000047961 |
HOGENOMi | CLU_047132_0_0_1 |
InParanoidi | Q8BT07 |
OMAi | SNIHEME |
OrthoDBi | 850483at2759 |
PhylomeDBi | Q8BT07 |
TreeFami | TF331107 |
Family and domain databases
InterProi | View protein in InterPro IPR038926, CEP55 IPR022008, EABR |
PANTHERi | PTHR31838, PTHR31838, 1 hit |
Pfami | View protein in Pfam PF12180, EABR, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSSRSPKDLI KSKWGSRPSS SKSDTALEKF KGEIAAFKTS LDEITSGKGK
60 70 80 90 100
MAEKGRSRLL EKIQVLEAER EKNVYYLLEK DKEIQRLKDH LRSRYSSSSL
110 120 130 140 150
FEQLEEKTKE CEKKQQLLES LSKETDVLKN QLSATTKRLS ELESKASTLH
160 170 180 190 200
LSQSMPANCF NSSMNSIHEK EMQLKDALEK NQQWLVYDQQ REAYVKGLLA
210 220 230 240 250
KIFELEKRTE TAAASLTQQM KKIESEGYLQ VEKQKYDHLL ENAKKDLEVE
260 270 280 290 300
RQAVTQLRLE LDEFRRKYEE ARKEVEDLNQ LLSSQRKADI QHLEEDKQKT
310 320 330 340 350
ERIQKLREES SIFKGKLEEE RKRSEELLSQ VRILYDSLLK HQEEQARVAL
360 370 380 390 400
LEQQMQACTL DFENEKLDRQ NMQHQLYVIL KELRKAKSQI TQLESLKQLH
410 420 430 440 450
GFTITEQPFP LQREPESRVK ATSPKSPSAA LNDSLVECPK CSVQYPATEH
460
RDLLVHVEYC MK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 70 | R → G in BAC40417 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 106 | E → K in BAC40417 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 157 | A → P in BAC25819 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 253 | A → T in AAH26966 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 259 | L → V in AAH26966 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 300 | T → M in AAH26966 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 406 | E → D in BAC25819 (PubMed:16141072).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 55 | G → D2 Publications | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_018630 | 397 | Missing in isoform 2. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK004655 mRNA Translation: BAB23446.1 AK028216 mRNA Translation: BAC25819.1 AK088548 mRNA Translation: BAC40417.1 BC026966 mRNA Translation: AAH26966.1 BC031396 mRNA Translation: AAH31396.1 |
CCDSi | CCDS29782.1 [Q8BT07-2] CCDS50429.1 [Q8BT07-1] |
RefSeqi | NP_001157834.1, NM_001164362.1 [Q8BT07-1] NP_082569.1, NM_028293.1 [Q8BT07-1] NP_083036.2, NM_028760.2 [Q8BT07-2] XP_006527459.1, XM_006527396.2 [Q8BT07-1] XP_006527460.1, XM_006527397.3 [Q8BT07-1] XP_006527461.1, XM_006527398.3 [Q8BT07-1] XP_006527462.1, XM_006527399.2 [Q8BT07-1] XP_006527463.1, XM_006527400.2 XP_006527464.1, XM_006527401.2 [Q8BT07-1] XP_011245683.1, XM_011247381.2 [Q8BT07-1] |
Genome annotation databases
Ensembli | ENSMUST00000096096; ENSMUSP00000093802; ENSMUSG00000024989 [Q8BT07-1] ENSMUST00000116506; ENSMUSP00000112205; ENSMUSG00000024989 [Q8BT07-2] ENSMUST00000169673; ENSMUSP00000127961; ENSMUSG00000024989 [Q8BT07-1] ENSMUST00000236044; ENSMUSP00000157898; ENSMUSG00000024989 [Q8BT07-1] ENSMUST00000237408; ENSMUSP00000157871; ENSMUSG00000024989 [Q8BT07-2] |
GeneIDi | 74107 |
KEGGi | mmu:74107 |
UCSCi | uc008hiy.2, mouse [Q8BT07-1] uc008hja.2, mouse [Q8BT07-2] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK004655 mRNA Translation: BAB23446.1 AK028216 mRNA Translation: BAC25819.1 AK088548 mRNA Translation: BAC40417.1 BC026966 mRNA Translation: AAH26966.1 BC031396 mRNA Translation: AAH31396.1 |
CCDSi | CCDS29782.1 [Q8BT07-2] CCDS50429.1 [Q8BT07-1] |
RefSeqi | NP_001157834.1, NM_001164362.1 [Q8BT07-1] NP_082569.1, NM_028293.1 [Q8BT07-1] NP_083036.2, NM_028760.2 [Q8BT07-2] XP_006527459.1, XM_006527396.2 [Q8BT07-1] XP_006527460.1, XM_006527397.3 [Q8BT07-1] XP_006527461.1, XM_006527398.3 [Q8BT07-1] XP_006527462.1, XM_006527399.2 [Q8BT07-1] XP_006527463.1, XM_006527400.2 XP_006527464.1, XM_006527401.2 [Q8BT07-1] XP_011245683.1, XM_011247381.2 [Q8BT07-1] |
3D structure databases
SMRi | Q8BT07 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 216498, 42 interactors |
IntActi | Q8BT07, 49 interactors |
STRINGi | 10090.ENSMUSP00000127961 |
PTM databases
iPTMneti | Q8BT07 |
PhosphoSitePlusi | Q8BT07 |
Proteomic databases
EPDi | Q8BT07 |
jPOSTi | Q8BT07 |
PaxDbi | Q8BT07 |
PeptideAtlasi | Q8BT07 |
PRIDEi | Q8BT07 |
Protocols and materials databases
Antibodypediai | 30468, 299 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000096096; ENSMUSP00000093802; ENSMUSG00000024989 [Q8BT07-1] ENSMUST00000116506; ENSMUSP00000112205; ENSMUSG00000024989 [Q8BT07-2] ENSMUST00000169673; ENSMUSP00000127961; ENSMUSG00000024989 [Q8BT07-1] ENSMUST00000236044; ENSMUSP00000157898; ENSMUSG00000024989 [Q8BT07-1] ENSMUST00000237408; ENSMUSP00000157871; ENSMUSG00000024989 [Q8BT07-2] |
GeneIDi | 74107 |
KEGGi | mmu:74107 |
UCSCi | uc008hiy.2, mouse [Q8BT07-1] uc008hja.2, mouse [Q8BT07-2] |
Organism-specific databases
CTDi | 55165 |
MGIi | MGI:1921357, Cep55 |
Phylogenomic databases
eggNOGi | ENOG502QTDI, Eukaryota |
GeneTreei | ENSGT00510000047961 |
HOGENOMi | CLU_047132_0_0_1 |
InParanoidi | Q8BT07 |
OMAi | SNIHEME |
OrthoDBi | 850483at2759 |
PhylomeDBi | Q8BT07 |
TreeFami | TF331107 |
Miscellaneous databases
BioGRID-ORCSi | 74107, 2 hits in 17 CRISPR screens |
ChiTaRSi | Cep55, mouse |
PROi | PR:Q8BT07 |
RNActi | Q8BT07, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000024989, Expressed in embryo and 212 other tissues |
Genevisiblei | Q8BT07, MM |
Family and domain databases
InterProi | View protein in InterPro IPR038926, CEP55 IPR022008, EABR |
PANTHERi | PTHR31838, PTHR31838, 1 hit |
Pfami | View protein in Pfam PF12180, EABR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CEP55_MOUSE | |
Accessioni | Q8BT07Primary (citable) accession number: Q8BT07 Secondary accession number(s): Q8C2J0 Q9DBZ8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2006 |
Last sequence update: | May 30, 2006 | |
Last modified: | December 2, 2020 | |
This is version 131 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot