Cep55

Functionⁱ

Plays a role in mitotic exit and cytokinesis. Recruits PDCD6IP and TSG101 to midbody during cytokinesis. Required for successful completion of cytokinesis. Not required for microtubule nucleation. Plays a role in the development of the brain and kidney.By similarity

GO - Biological processⁱ

cell separation after cytokinesis Source: MGI
cranial skeletal system development Source: UniProtKB
establishment of protein localization Source: MGI
mitotic cytokinesis Source: MGI
regulation of phosphatidylinositol 3-kinase signaling Source: InterPro
renal system development Source: UniProtKB

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Cell cycle, Cell division, Mitosis

Biological process

Cell cycle, Cell division, Mitosis

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Centrosomal protein of 55 kDaImported Short name: Cep55
Gene namesⁱ	Name:Cep55Imported
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 19

Organism-specific databases

MGIⁱ	MGI:1921357 Cep55

Keywords - Cellular componentⁱ

Cytoplasm, Cytoskeleton

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000238665	1 – 462	Centrosomal protein of 55 kDaAdd BLAST		462

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	96	PhosphoserineCombined sources	1
Modified residueⁱ	99	PhosphoserineBy similarity	1
Modified residueⁱ	423	PhosphoserineBy similarity	1
Modified residueⁱ	426	PhosphoserineCombined sources	1
Modified residueⁱ	428	PhosphoserineCombined sources	1
Modified residueⁱ	434	Phosphoserine; by PLK1Combined sources	1

Post-translational modificationⁱ

There is a hierachy of phosphorylation, where both Ser-423 and Ser-426 are phosphorylated at the onset of mitosis, prior to Ser-434. Phosphorylation at Ser-423 and Ser-426 is required for dissociation from the centrosome at the G2/M boundary. Phosphorylation at the 3 sites, Ser-423, Ser-426 and Ser-434, is required for protein function at the final stages of cell division to complete cytokinesis successfully (By similarity).By similarity

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

EPDⁱ	Q8BT07
PaxDbⁱ	Q8BT07
PeptideAtlas More...PeptideAtlasⁱ	Q8BT07
PRIDEⁱ	Q8BT07

PTM databases

iPTMnetⁱ	Q8BT07
PhosphoSitePlusⁱ	Q8BT07

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000024989 Expressed in 193 organ(s), highest expression level in embryo
CleanExⁱ	MM_CEP55
Genevisibleⁱ	Q8BT07 MM

Interactionⁱ

Subunit structureⁱ

Homodimer. Interacts (phosphorylated on Ser-423 and Ser-426) with PLK1. Interacts with AKAP9/CG-NAP; the interaction occurs in interphase and is lost upon mitotic entry. Interacts with PCNT/Kendrin; the interaction occurs in interphase and is lost upon mitotic entry. Directly interacts with PDCD6IP; this interaction is required for PDCD6IP targeting to the midbody; CEP55 binds PDCD6IP in a 2:1 stoichiometry; PDCD6IP competes with TSG101 for the same binding site. Interacts with TSG101; TSG101 competes with PDCD6IP for the same binding site; interaction is required for cytokinesis. Interacts with MVB12A, VPS37B, VPS37C and VPS28 (By similarity).By similarity

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
Tex14	Q7M6U3	11	EBI-2552328,EBI-6674575

With

Entry

#Exp.

IntAct

Notes

Tex14

Q7M6U3

11

EBI-2552328,EBI-6674575

Protein-protein interaction databases

BioGridⁱ	216498, 42 interactors
IntActⁱ	Q8BT07, 49 interactors
STRINGⁱ	10090.ENSMUSP00000093802

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q8BT07
SMRⁱ	Q8BT07
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	157 – 235	Interaction with TSG101By similarityAdd BLAST	79
Regionⁱ	160 – 214	Interaction with PDCD6IPBy similarityAdd BLAST	55
Regionⁱ	354 – 462	Required for localization to the interphase centrosome and to the midbody during cytokinesisBy similarityAdd BLAST	109

Coiled coil

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Coiled coilⁱ	50 – 400	Sequence analysisAdd BLAST		351

Keywords - Domainⁱ

Coiled coil

Phylogenomic databases

eggNOGⁱ	ENOG410IFBZ Eukaryota ENOG41120Y2 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00510000047961
HOGENOMⁱ	HOG000111547
HOVERGENⁱ	HBG081092
InParanoidⁱ	Q8BT07
KEGG Orthology (KO) More...KOⁱ	K16456
OMAⁱ	YDQQREV
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G065Y
PhylomeDBⁱ	Q8BT07
TreeFamⁱ	TF331107

Family and domain databases

InterProⁱ	View protein in InterPro IPR038926 CEP55 IPR022008 EABR
PANTHERⁱ	PTHR31838 PTHR31838, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF12180 EABR, 1 hit

Sequences (2)ⁱ

Sequence statusⁱ: Complete.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket

Isoform 1 (identifier: Q8BT07-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSRSPKDLI KSKWGSRPSS SKSDTALEKF KGEIAAFKTS LDEITSGKGK 
        60         70         80         90        100
MAEKGRSRLL EKIQVLEAER EKNVYYLLEK DKEIQRLKDH LRSRYSSSSL 
       110        120        130        140        150
FEQLEEKTKE CEKKQQLLES LSKETDVLKN QLSATTKRLS ELESKASTLH 
       160        170        180        190        200
LSQSMPANCF NSSMNSIHEK EMQLKDALEK NQQWLVYDQQ REAYVKGLLA 
       210        220        230        240        250
KIFELEKRTE TAAASLTQQM KKIESEGYLQ VEKQKYDHLL ENAKKDLEVE 
       260        270        280        290        300
RQAVTQLRLE LDEFRRKYEE ARKEVEDLNQ LLSSQRKADI QHLEEDKQKT 
       310        320        330        340        350
ERIQKLREES SIFKGKLEEE RKRSEELLSQ VRILYDSLLK HQEEQARVAL 
       360        370        380        390        400
LEQQMQACTL DFENEKLDRQ NMQHQLYVIL KELRKAKSQI TQLESLKQLH 
       410        420        430        440        450
GFTITEQPFP LQREPESRVK ATSPKSPSAA LNDSLVECPK CSVQYPATEH 
       460 
RDLLVHVEYC MK

Length:462

Mass (Da):53,930

Last modified:May 30, 2006 - v2

Checksum:ⁱ5D2308BFCEADA08B

GO

Isoform 2 (identifier: Q8BT07-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
397-397: Missing.

« Hide

        10         20         30         40         50
MSSRSPKDLI KSKWGSRPSS SKSDTALEKF KGEIAAFKTS LDEITSGKGK 
        60         70         80         90        100
MAEKGRSRLL EKIQVLEAER EKNVYYLLEK DKEIQRLKDH LRSRYSSSSL 
       110        120        130        140        150
FEQLEEKTKE CEKKQQLLES LSKETDVLKN QLSATTKRLS ELESKASTLH 
       160        170        180        190        200
LSQSMPANCF NSSMNSIHEK EMQLKDALEK NQQWLVYDQQ REAYVKGLLA 
       210        220        230        240        250
KIFELEKRTE TAAASLTQQM KKIESEGYLQ VEKQKYDHLL ENAKKDLEVE 
       260        270        280        290        300
RQAVTQLRLE LDEFRRKYEE ARKEVEDLNQ LLSSQRKADI QHLEEDKQKT 
       310        320        330        340        350
ERIQKLREES SIFKGKLEEE RKRSEELLSQ VRILYDSLLK HQEEQARVAL 
       360        370        380        390        400
LEQQMQACTL DFENEKLDRQ NMQHQLYVIL KELRKAKSQI TQLESLQLHG 
       410        420        430        440        450
FTITEQPFPL QREPESRVKA TSPKSPSAAL NDSLVECPKC SVQYPATEHR 
       460 
DLLVHVEYCM K

Note: No experimental confirmation available.

Show »

Length:461

Mass (Da):53,802

Checksum:ⁱC0816F19989B4FA1

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	70	R → G in BAC40417 (PubMed:16141072).Curated	1
Sequence conflictⁱ	106	E → K in BAC40417 (PubMed:16141072).Curated	1
Sequence conflictⁱ	157	A → P in BAC25819 (PubMed:16141072).Curated	1
Sequence conflictⁱ	253	A → T in AAH26966 (PubMed:15489334).Curated	1
Sequence conflictⁱ	259	L → V in AAH26966 (PubMed:15489334).Curated	1
Sequence conflictⁱ	300	T → M in AAH26966 (PubMed:15489334).Curated	1
Sequence conflictⁱ	406	E → D in BAC25819 (PubMed:16141072).Curated	1

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱ	55	G → D2 Publications		1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_018630	397	Missing in isoform 2. 1 Publication		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK004655 mRNA Translation: BAB23446.1 AK028216 mRNA Translation: BAC25819.1 AK088548 mRNA Translation: BAC40417.1 BC026966 mRNA Translation: AAH26966.1 BC031396 mRNA Translation: AAH31396.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS29782.1 [Q8BT07-2] CCDS50429.1 [Q8BT07-1]
NCBI Reference Sequences More...RefSeqⁱ	NP_001157834.1, NM_001164362.1 [Q8BT07-1] NP_082569.1, NM_028293.1 [Q8BT07-1] NP_083036.2, NM_028760.2 [Q8BT07-2] XP_006527459.1, XM_006527396.2 [Q8BT07-1] XP_006527460.1, XM_006527397.3 [Q8BT07-1] XP_006527461.1, XM_006527398.3 [Q8BT07-1] XP_006527462.1, XM_006527399.2 [Q8BT07-1] XP_006527463.1, XM_006527400.2 XP_006527464.1, XM_006527401.2 [Q8BT07-1] XP_011245683.1, XM_011247381.2 [Q8BT07-1]
UniGeneⁱ	Mm.9916

Genome annotation databases

Ensemblⁱ	ENSMUST00000096096; ENSMUSP00000093802; ENSMUSG00000024989 [Q8BT07-1] ENSMUST00000116506; ENSMUSP00000112205; ENSMUSG00000024989 [Q8BT07-2] ENSMUST00000169673; ENSMUSP00000127961; ENSMUSG00000024989 [Q8BT07-1]
GeneIDⁱ	74107
KEGGⁱ	mmu:74107
UCSC genome browser More...UCSCⁱ	uc008hiy.2 mouse [Q8BT07-1] uc008hja.2 mouse [Q8BT07-2]

Keywords - Coding sequence diversityⁱ

Alternative splicing, Polymorphism

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q8BT07	Centrosomal protein of 55 kDa		RAT		462	UniRef90_Q8BT07
Isoform 2 of Centrosomal protein of 55 kDa		MOUSE		461
UPI000A310666		MUSPA		462
UPI000A31929B		MUSCR		462
UPI0003D7149C		MOUSE		308
+2

Protein	Similar proteins		Species	Score	Length	Source
Q8BT07	Isoform 2 of Centrosomal protein of 55 kDa		HUMAN		400	UniRef50_Q53EZ4-2
Centrosomal protein of 55 kDa		RAT		462
Centrosomal protein 55		RHIBE		487
Centrosomal protein 55		CERAT		464
Centrosomal protein of 55 kDa		MACMU		464
+109

Entry informationⁱ

Entry nameⁱ	CEP55_MOUSE
Accessionⁱ	Q8BT07Primary (citable) accession number: Q8BT07 Secondary accession number(s): Q8C2J0 , Q8K2I8, Q8R2Y4, Q9DBZ8
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
	Last sequence update:	May 30, 2006
	Last modified:	September 12, 2018
	This is version 116 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q8BT07 (CEP55_MOUSE)

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Centrosomal protein of 55 kDa

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Cytoskeleton

Other locations

Cytoskeleton

Extracellular region or secreted

Plasma Membrane

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Coiled coil

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Natural variant

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Domainⁱ

Keywords - Coding sequence diversityⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ