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Protein

E3 ubiquitin-protein ligase pellino homolog 2

Gene

Peli2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Mediates IL1B-induced IRAK1 'Lys-63'-linked polyubiquitination and possibly 'Lys-48'-linked ubiquitination. May be important for LPS- and IL1B-induced MAP3K7-dependent, but not MAP3K3-dependent, NF-kappa-B activation. Can activate the MAP (mitogen activated protein) kinase pathway leading to activation of ELK1.2 Publications

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • protein binding, bridging Source: UniProtKB
  • ubiquitin protein ligase activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway

Enzyme and pathway databases

UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase pellino homolog 2 (EC:2.3.2.27)
Short name:
Pellino-2
Alternative name(s):
RING-type E3 ubiquitin transferase pellino homolog 2Curated
Gene namesi
Name:Peli2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1891445 Peli2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001941751 – 419E3 ubiquitin-protein ligase pellino homolog 2Add BLAST419

Post-translational modificationi

Phosphorylated by IRAK1 and IRAK4 enhancing its E3 ligase activity.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8BST6
PRIDEiQ8BST6

PTM databases

iPTMnetiQ8BST6
PhosphoSitePlusiQ8BST6

Expressioni

Tissue specificityi

Widely expressed both in embryos and adult. Weakly or not expressed in spleen and thymus.1 Publication

Gene expression databases

CleanExiMM_PELI2

Interactioni

Subunit structurei

Interacts with TRAF6, IRAK4 and MAP3K7 (By similarity). Interacts with IRAK1. Interacts with BCL10; this interaction is impaired by SOCS3.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Irak1Q624062EBI-448554,EBI-448533

GO - Molecular functioni

Protein-protein interaction databases

BioGridi220309, 3 interactors
IntActiQ8BST6, 1 interactor
STRINGi10090.ENSMUSP00000072894

Structurei

3D structure databases

ProteinModelPortaliQ8BST6
SMRiQ8BST6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 202FHA; atypicalAdd BLAST188

Domaini

The atypical FHA domain contains a 'wing' insert and mediates binding to threonine-phosphorylated IRAK1.By similarity

Sequence similaritiesi

Belongs to the pellino family.Curated

Phylogenomic databases

eggNOGiKOG3842 Eukaryota
ENOG410XP3S LUCA
HOGENOMiHOG000234110
HOVERGENiHBG053559
InParanoidiQ8BST6
KOiK11964
PhylomeDBiQ8BST6

Family and domain databases

InterProiView protein in InterPro
IPR006800 Pellino_fam
PANTHERiPTHR12098 PTHR12098, 1 hit
PfamiView protein in Pfam
PF04710 Pellino, 1 hit
PIRSFiPIRSF038886 Pellino, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8BST6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MFSPGQEEPS APNKEPVKYR ELVVLGYNGA LPNGDRGRRK SRFALYKRTY
60 70 80 90 100
ASGVKPSTIH MVSTPQASKA ISSRGHHSIS YTLSRSQTVV VEYTHDKDTD
110 120 130 140 150
MFQVGRSTES PIDFVVTDTV SGGQNEDAQI TQSTISRFAC RIVCDRNEPY
160 170 180 190 200
TARIFAAGFD SSKNIFLGEK AAKWKNPDGH MDGLTTNGVL VMHPQGGFTE
210 220 230 240 250
ESQPGVWREI SVCGDVYTLR ETRSAQQRGK LVESETNVLQ DGSLIDLCGA
260 270 280 290 300
TLLWRTADGL FHAPTQKHIE ALRQEINAAR PQCPVGLNTL AFPSINRKEV
310 320 330 340 350
VEEKQPWAYL SCGHVHGYHS WGHRSDTEAN ERECPMCRTV GPYVPLWLGC
360 370 380 390 400
EAGFYVDAGP PTHAFTPCGH VCSEKSAKYW SQIPLPHGTH AFHAACPFCA
410
TQLVGEQNCI KLIFQGPVD
Length:419
Mass (Da):46,272
Last modified:October 24, 2003 - v2
Checksum:i786C92C28C38D0CB
GO
Isoform 2 (identifier: Q8BST6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-100: Missing.

Note: No experimental confirmation available.
Show »
Length:319
Mass (Da):35,165
Checksum:i7FFA0991F1FA0950
GO
Isoform 3 (identifier: Q8BST6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     70-155: AISSRGHHSI...RNEPYTARIF → LPAAKHYYNE...KKKIFFSCWS
     156-419: Missing.

Note: No experimental confirmation available.
Show »
Length:155
Mass (Da):17,403
Checksum:iE443ACF3246FA349
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9QPQ6E9QPQ6_MOUSE
E3 ubiquitin-protein ligase pellino...
Peli2
419Annotation score:
A0A2I3BQT4A0A2I3BQT4_MOUSE
E3 ubiquitin-protein ligase pellino...
Peli2
155Annotation score:
A0A2I3BRM8A0A2I3BRM8_MOUSE
E3 ubiquitin-protein ligase pellino...
Peli2
225Annotation score:
A0A2I3BRM2A0A2I3BRM2_MOUSE
E3 ubiquitin-protein ligase pellino...
Peli2
45Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7E → K in BAC27024 (PubMed:16141072).Curated1
Sequence conflicti11A → T in BAC27024 (PubMed:16141072).Curated1
Sequence conflicti20R → G in BAC38472 (PubMed:16141072).Curated1
Sequence conflicti38 – 39RR → KK in AAG15392 (PubMed:11306823).Curated2
Sequence conflicti44A → T in AAG15392 (PubMed:11306823).Curated1
Sequence conflicti320S → H in AAG15392 (PubMed:11306823).Curated1
Sequence conflicti327T → A in AAH27062 (PubMed:15489334).Curated1
Sequence conflicti338R → M in AAG15392 (PubMed:11306823).Curated1
Sequence conflicti364A → V in AAG15392 (PubMed:11306823).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0086361 – 100Missing in isoform 2. 1 PublicationAdd BLAST100
Alternative sequenceiVSP_00863770 – 155AISSR…TARIF → LPAAKHYYNEADSESLSALT LKVRDFLTGECSQRREYRDP AFSREGASGSAQLVAQAFLI CPLSYTIVKQEQIRCLKKKI FFSCWS in isoform 3. 1 PublicationAdd BLAST86
Alternative sequenceiVSP_008638156 – 419Missing in isoform 3. 1 PublicationAdd BLAST264

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302504 mRNA Translation: AAG15392.1
AK030564 mRNA Translation: BAC27024.1
AK033815 mRNA Translation: BAC28485.1
AK082342 mRNA Translation: BAC38472.1
BC027062 mRNA Translation: AAH27062.1
CCDSiCCDS36901.1 [Q8BST6-1]
RefSeqiNP_291080.2, NM_033602.2
XP_006519804.1, XM_006519741.2 [Q8BST6-2]
UniGeneiMm.296457

Genome annotation databases

GeneIDi93834
KEGGimmu:93834
UCSCiuc007tji.1 mouse [Q8BST6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302504 mRNA Translation: AAG15392.1
AK030564 mRNA Translation: BAC27024.1
AK033815 mRNA Translation: BAC28485.1
AK082342 mRNA Translation: BAC38472.1
BC027062 mRNA Translation: AAH27062.1
CCDSiCCDS36901.1 [Q8BST6-1]
RefSeqiNP_291080.2, NM_033602.2
XP_006519804.1, XM_006519741.2 [Q8BST6-2]
UniGeneiMm.296457

3D structure databases

ProteinModelPortaliQ8BST6
SMRiQ8BST6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi220309, 3 interactors
IntActiQ8BST6, 1 interactor
STRINGi10090.ENSMUSP00000072894

PTM databases

iPTMnetiQ8BST6
PhosphoSitePlusiQ8BST6

Proteomic databases

PaxDbiQ8BST6
PRIDEiQ8BST6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi93834
KEGGimmu:93834
UCSCiuc007tji.1 mouse [Q8BST6-1]

Organism-specific databases

CTDi57161
MGIiMGI:1891445 Peli2

Phylogenomic databases

eggNOGiKOG3842 Eukaryota
ENOG410XP3S LUCA
HOGENOMiHOG000234110
HOVERGENiHBG053559
InParanoidiQ8BST6
KOiK11964
PhylomeDBiQ8BST6

Enzyme and pathway databases

UniPathwayi
UPA00143

Miscellaneous databases

PROiPR:Q8BST6
SOURCEiSearch...

Gene expression databases

CleanExiMM_PELI2

Family and domain databases

InterProiView protein in InterPro
IPR006800 Pellino_fam
PANTHERiPTHR12098 PTHR12098, 1 hit
PfamiView protein in Pfam
PF04710 Pellino, 1 hit
PIRSFiPIRSF038886 Pellino, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPELI2_MOUSE
AccessioniPrimary (citable) accession number: Q8BST6
Secondary accession number(s): Q8C4F2
, Q8CC65, Q8R2X4, Q9ERJ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 24, 2003
Last sequence update: October 24, 2003
Last modified: November 7, 2018
This is version 111 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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