UniProtKB - Q8BQ48 (CE295_MOUSE)
Protein
Centrosomal protein of 295 kDa
Gene
Cep295
Organism
Mus musculus (Mouse)
Status
Functioni
Centriole-enriched microtubule-binding protein involved in centriole biogenesis. Essential for the generation of the distal portion of new-born centrioles in a CENPJ- and CEP120-mediated elongation dependent manner during the cell cycle S/G2 phase after formation of the initiating cartwheel structure. Required for the recruitment of centriolar proteins, such as POC1B, POC5 and CEP135, into the distal portion of centrioles. Also required for centriole-to-centrosome conversion during mitotic progression, but is dispensable for cartwheel removal or centriole disengagement. Binds to and stabilizes centriolar microtubule.By similarity
GO - Molecular functioni
- microtubule binding Source: UniProtKB
GO - Biological processi
- centriole replication Source: UniProtKB
- positive regulation of centriole elongation Source: UniProtKB
- positive regulation of centrosome duplication Source: UniProtKB
- positive regulation of establishment of protein localization Source: UniProtKB
- positive regulation of protein acetylation Source: UniProtKB
- regulation of centriole replication Source: GO_Central
Names & Taxonomyi
Protein namesi | Recommended name: Centrosomal protein of 295 kDaCurated |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2442521, Cep295 |
Subcellular locationi
Cytoskeleton
- centriole By similarity
- centrosome By similarity
- spindle By similarity
- cytoskeleton By similarity
Note: Associates with both of the converted centrioles during G1 but becomes more enriched at the newly formed daughter (or unconverted) centrioles during S, G2, and early M phases. In early S phase, localized at the procentriolar microtubule wall and enriched at the proximal ends of the centrioles in CENPJ- and CEP135-dependent manner. Colocalizes with SASS6 and CEP250 proteins. Colocalizes with CEP135 and CEP192 at the centrosomes. Associates with interphase microtubules and mitotic spindles. Colocalizes with centriolar acetylated tubulin.By similarity
Cytoskeleton
- centriole Source: UniProtKB
- centrosome Source: UniProtKB
- cytoskeleton Source: MGI
- mitotic spindle microtubule Source: UniProtKB
Cytosol
- cytosol Source: MGI
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- cytoplasm Source: MGI
Keywords - Cellular componenti
Cytoplasm, CytoskeletonPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000324596 | 1 – 2412 | Centrosomal protein of 295 kDaAdd BLAST | 2412 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 13 | PhosphoserineBy similarity | 1 | |
Modified residuei | 634 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1573 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q8BQ48 |
PRIDEi | Q8BQ48 |
PTM databases
iPTMneti | Q8BQ48 |
PhosphoSitePlusi | Q8BQ48 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000046111, Expressed in forelimb bud and 226 other tissues |
Interactioni
Subunit structurei
Interacts (via ALMS motif) with microtubules; this interaction is direct.
By similarityGO - Molecular functioni
- microtubule binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 235442, 6 interactors |
STRINGi | 10090.ENSMUSP00000123788 |
Miscellaneous databases
RNActi | Q8BQ48, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 540 | Necessary for centriole targeting and microtubule associationBy similarityAdd BLAST | 540 | |
Regioni | 2367 – 2412 | ALMS motifAdd BLAST | 46 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 63 – 84 | Sequence analysisAdd BLAST | 22 | |
Coiled coili | 114 – 134 | Sequence analysisAdd BLAST | 21 | |
Coiled coili | 209 – 273 | Sequence analysisAdd BLAST | 65 | |
Coiled coili | 489 – 535 | Sequence analysisAdd BLAST | 47 | |
Coiled coili | 563 – 592 | Sequence analysisAdd BLAST | 30 | |
Coiled coili | 811 – 841 | Sequence analysisAdd BLAST | 31 | |
Coiled coili | 1300 – 1327 | Sequence analysisAdd BLAST | 28 | |
Coiled coili | 1448 – 1493 | Sequence analysisAdd BLAST | 46 |
Domaini
The N-terminal and the ALMS motif-containing C-terminal regions are essential for CEP295-mediated centriole elongation.By similarity
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | ENOG502QSZR, Eukaryota |
HOGENOMi | CLU_001093_0_0_1 |
InParanoidi | Q8BQ48 |
OrthoDBi | 97863at2759 |
PhylomeDBi | Q8BQ48 |
TreeFami | TF331536 |
Family and domain databases
InterProi | View protein in InterPro IPR029560, CEP295 |
PANTHERi | PTHR21553:SF25, PTHR21553:SF25, 1 hit |
s (6+)i Sequence
Sequence statusi: Complete.
This entry describes 6 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 6 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8BQ48-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MKRKGMNTKL RLSPSEEAFI LKEDYERRRK LRLLQVREQE RGIAFQIREG
60 70 80 90 100
IKQRRSQQVS HLAEELRAKW EEAQSQKIQN LEKLYLASLR HMGDGHRQAK
110 120 130 140 150
ENEPDLDALS RRAAERKRKA EVRHKEALKV QKNQKEMLMK QKTRHIKARK
160 170 180 190 200
EAVLVEKQRS AKMARLPPPV PSPFENIDVN RIPSLKTNSS TYHHISTFVS
210 220 230 240 250
RQMGTKQPDA HLAAEEEARR VERLRKQAAQ ERVKQFERAH VRGSQAMKKI
260 270 280 290 300
HLAQNQERLM EELKQLQKED LARRRQTVAQ MPPQMLELPY RRSEMKEDRQ
310 320 330 340 350
RELEFAFEDM YNADRKVKGN LILHLKPEPL PTISDQLQDE ELDLSMEQEN
360 370 380 390 400
QVPLAAKIQQ IPSRILFKRL LNKIRSQKSL WTIKSVSEDE GEVTSSIIEI
410 420 430 440 450
ESKVPSVDSG AIITEERTAA SFEQEQVTDS DRLTIESGPL SSEDKPLYYK
460 470 480 490 500
AGTGREQAMA VSPPATAVAQ SSVLLHPQEE AVRIRMSLRR KQIMEIEEQK
510 520 530 540 550
QKQLELLEQI EQQKLRLETD CFRAQLEEQR KQADQPEVCC APMSHAMISD
560 570 580 590 600
EDSHRQMIRN YQHQLLQQNR LHKETVETAR KRLLEYQTVL KERSPSLSAS
610 620 630 640 650
ALVPDSVVSG PPQQSYKPAA ASDSWDPSQR LKLSPSKYQP VQPSQIPALE
660 670 680 690 700
QSHIQVPRHG HITQRQGKMA VSEMLGKQPV ESQERQWQFS QVETHQGDYE
710 720 730 740 750
FVLKDSHSLS RTLSYVRPQT LQDAREVSKP PRVIICQSLD SQQISSEDSE
760 770 780 790 800
NISSKPSEPS PFLPLVPERP FTSLPVKFHS GTIHKPFTTI NQSVISQMHD
810 820 830 840 850
QPLSSSETIT AQQGDLRFLQ EQLELQKKVL QARQEAREKL LLCTQKELGQ
860 870 880 890 900
QTGLPVFLPS PAGNIFSSLP SASAESGNFQ TSSTKSDATV SSDNMDRLWD
910 920 930 940 950
SSQPISSQQT HLEFLQEQSS VETDNLQARR EAQEVLFAHT QNTLEKIVRS
960 970 980 990 1000
EQAGSSLPHQ VAQQSFSSLT LADTQSKKIQ KQPLPANKKG LLPSQSEVSK
1010 1020 1030 1040 1050
AQDGSSGFLQ QTLPLQNTLK LLQEQLTRQR SMIPPRRDGQ ETLLLYKESC
1060 1070 1080 1090 1100
SEDSEAGPVE SLSSVVVQHA DASRAVSEVP KRLQDVYSSE EENRVLSSHL
1110 1120 1130 1140 1150
ITHGFPQHSL QRQEHFTPLQ EETHIQRLIL GARKNNEEFA PKQNELEKGL
1160 1170 1180 1190 1200
CSQQTDALSS PSQVTDWGTS RGSVSVRSDR TDPLRHFKIP AFRERLVRVS
1210 1220 1230 1240 1250
QHTFPLQDNL QEHQEWVDTE KESFQSSPLT PENPSSQQTG FSSFKASLRL
1260 1270 1280 1290 1300
PSCVSLPSAD SGITQHPLST ESDSKVKSSH LQIPELQHRL SKISQLIPPQ
1310 1320 1330 1340 1350
QDSLKALQEQ LATQREAIIH SRQEAHEETL REWKEKIFPE QVGPFSPLIP
1360 1370 1380 1390 1400
QHSLASFPVS DTERAQELCS TNSDTISSGY PEMLELPDRT LGLSHTALPQ
1410 1420 1430 1440 1450
QNNLTAHPEH LHAQTNFFHS TEKAQEGLVF PRPCQFEEMS AEHFIQPQHD
1460 1470 1480 1490 1500
DLKALQQQLD MQREAIRSGQ EMQEKMLLQR LNKLEQRISS KQISSSLFSS
1510 1520 1530 1540 1550
QVALPIANSD GTLQSFPTKS NETELLGSQD EYLSFSQPRL PLQNNMTEQL
1560 1570 1580 1590 1600
DLEKVFHKEL LLHKQKSQNK SESSEHSLPP LFLSKEIEHP FISLPFAESK
1610 1620 1630 1640 1650
SKSICELYLS DKKHAAPNDA VIPRLQDRLL SCSQPVLTQQ DNMSLQKQLN
1660 1670 1680 1690 1700
LQRETLHSRQ KAQEELLVQR QTSLQQQIQR HRETLKNFFN VSQARNPTDE
1710 1720 1730 1740 1750
NDLEMQKREQ LGGWFPHTQG LTWGDAGQGS ANGEQPRADV HAEHNGESLA
1760 1770 1780 1790 1800
KELSGRASKP PVSKVKCVLD LNQHELSTIQ EVESPASGRI SMPGKAEFYQ
1810 1820 1830 1840 1850
DRDPLRVSVS REQSFLESPL AHDPFGCHQP PAQENSKSHD DNAEAVKVKK
1860 1870 1880 1890 1900
SDVEDHAVLS HAVSKEEACT NLGPLGKPDD EAETQEISQE PLSSVTVSTG
1910 1920 1930 1940 1950
SFLSYEITDL SLTDPESFSE QTEHLEQEST NKQEETDPLS IAVPSVIYQQ
1960 1970 1980 1990 2000
QHSLGAHNSL LPMEEESTSD HTHVQQIMDN DVNEANLIPD KRDFQVPAVD
2010 2020 2030 2040 2050
LDFRELEHIF PHLHRQLFKP LEPHLDFDLS SPGTSQEDSD FYQSSESSSE
2060 2070 2080 2090 2100
KHVKALSTGT ICFPALTAKS HSPNPRLNQL DINLAHATTE GSEQSFQQLR
2110 2120 2130 2140 2150
PEFSSQESQH ADLPSIYSIE ARGPSQRMEN QNYSEMLQNK KKSLSLQPST
2160 2170 2180 2190 2200
EDLTPACSSS DTALFDQLHL QHSTPCASVS SECSVKLLES REEVLGFEEL
2210 2220 2230 2240 2250
SRRAVTMSQR LTEDENVVLP INPHVGRVEK EASVQGSNPL SIQNEKPIQN
2260 2270 2280 2290 2300
FIETDTTEAV GNVCQLAQAE HILKSCPFRS PIPIWETDTG YGIMEEPDLT
2310 2320 2330 2340 2350
LVSNSDISIT ETDLANLTLE DREDNEAQFF QAGVVLPTSS METSVCGAVS
2360 2370 2380 2390 2400
EPYVDQPTVA PSATSGSLQE AFMTRQTLTE RSYQRQREIW NKTRLPQTKV
2410
SKEKLPTGCT GS
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9QPW4 | E9QPW4_MOUSE | Centrosomal protein of 295 kDa | Cep295 5830418K08Rik | 2,380 | Annotation score: | ||
F7BWD7 | F7BWD7_MOUSE | Centrosomal protein of 295 kDa | Cep295 5830418K08Rik | 1,202 | Annotation score: | ||
A0A1N9NPH8 | A0A1N9NPH8_MOUSE | Centrosomal protein of 295 kDa | Cep295 | 2,460 | Annotation score: | ||
F6V9M5 | F6V9M5_MOUSE | Centrosomal protein of 295 kDa | Cep295 5830418K08Rik | 257 | Annotation score: | ||
F6URK1 | F6URK1_MOUSE | Centrosomal protein of 295 kDa | Cep295 5830418K08Rik | 111 | Annotation score: | ||
A0A1L1STC4 | A0A1L1STC4_MOUSE | Centrosomal protein of 295 kDa | Cep295 | 70 | Annotation score: |
Sequence cautioni
The sequence AAI18969 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence BAC27153 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence BAD90195 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 1344 | P → T in BAC27977 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 1872 | L → M in BAC27977 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 1924 | H → R in BAC27153 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_032292 | 655 – 1237 | Missing in isoform 4. 1 PublicationAdd BLAST | 583 | |
Alternative sequenceiVSP_032293 | 1341 – 1692 | Missing in isoform 4. 1 PublicationAdd BLAST | 352 | |
Alternative sequenceiVSP_032294 | 1916 – 1995 | Missing in isoform 3, isoform 4 and isoform 6. 3 PublicationsAdd BLAST | 80 | |
Alternative sequenceiVSP_032295 | 2042 | Y → YQ in isoform 5. 1 Publication | 1 | |
Alternative sequenceiVSP_032296 | 2286 – 2287 | ET → VS in isoform 5 and isoform 6. 1 Publication | 2 | |
Alternative sequenceiVSP_032297 | 2288 – 2412 | Missing in isoform 5 and isoform 6. 1 PublicationAdd BLAST | 125 | |
Alternative sequenceiVSP_032298 | 2363 – 2412 | ATSGS…GCTGS → GKPLISLRTLSTHVGMKATH NFSQIL in isoform 2. 1 PublicationAdd BLAST | 50 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC154296 Genomic DNA No translation available. AK030838 mRNA Translation: BAC27153.1 Different initiation. AK032664 mRNA Translation: BAC27977.1 AK051561 mRNA Translation: BAC34674.2 AK134513 mRNA Translation: BAE22166.1 AK220270 mRNA Translation: BAD90195.1 Different initiation. BC118968 mRNA Translation: AAI18969.1 Different initiation. |
RefSeqi | NP_795950.2, NM_176976.4 |
Genome annotation databases
GeneIDi | 319675 |
KEGGi | mmu:319675 |
UCSCi | uc009ofv.2, mouse [Q8BQ48-4] uc009ofw.3, mouse [Q8BQ48-1] uc009ofy.2, mouse [Q8BQ48-6] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC154296 Genomic DNA No translation available. AK030838 mRNA Translation: BAC27153.1 Different initiation. AK032664 mRNA Translation: BAC27977.1 AK051561 mRNA Translation: BAC34674.2 AK134513 mRNA Translation: BAE22166.1 AK220270 mRNA Translation: BAD90195.1 Different initiation. BC118968 mRNA Translation: AAI18969.1 Different initiation. |
RefSeqi | NP_795950.2, NM_176976.4 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 235442, 6 interactors |
STRINGi | 10090.ENSMUSP00000123788 |
PTM databases
iPTMneti | Q8BQ48 |
PhosphoSitePlusi | Q8BQ48 |
Proteomic databases
PaxDbi | Q8BQ48 |
PRIDEi | Q8BQ48 |
Genome annotation databases
GeneIDi | 319675 |
KEGGi | mmu:319675 |
UCSCi | uc009ofv.2, mouse [Q8BQ48-4] uc009ofw.3, mouse [Q8BQ48-1] uc009ofy.2, mouse [Q8BQ48-6] |
Organism-specific databases
CTDi | 85459 |
MGIi | MGI:2442521, Cep295 |
Rougei | Search... |
Phylogenomic databases
eggNOGi | ENOG502QSZR, Eukaryota |
HOGENOMi | CLU_001093_0_0_1 |
InParanoidi | Q8BQ48 |
OrthoDBi | 97863at2759 |
PhylomeDBi | Q8BQ48 |
TreeFami | TF331536 |
Miscellaneous databases
BioGRID-ORCSi | 319675, 4 hits in 17 CRISPR screens |
ChiTaRSi | Cep295, mouse |
PROi | PR:Q8BQ48 |
RNActi | Q8BQ48, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000046111, Expressed in forelimb bud and 226 other tissues |
Family and domain databases
InterProi | View protein in InterPro IPR029560, CEP295 |
PANTHERi | PTHR21553:SF25, PTHR21553:SF25, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CE295_MOUSE | |
Accessioni | Q8BQ48Primary (citable) accession number: Q8BQ48 Secondary accession number(s): Q0VF60 Q8CDA9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 18, 2008 |
Last sequence update: | March 18, 2008 | |
Last modified: | December 2, 2020 | |
This is version 106 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot