Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

L-seryl-tRNA(Sec) kinase

Gene

Pstk

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Specifically phosphorylates seryl-tRNA(Sec) to O-phosphoseryl-tRNA(Sec), an activated intermediate for selenocysteine biosynthesis. No activity with other tRNAs has been detected.

Catalytic activityi

ATP + L-seryl-tRNA(Sec) = ADP + O-phospho-L-seryl-tRNA(Sec).

Cofactori

Pathwayi: selenocysteinyl-tRNA(Sec) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes selenocysteinyl-tRNA(Sec) from L-seryl-tRNA(Sec) (archaeal/eukaryal route).
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. L-seryl-tRNA(Sec) kinase (Pstk)
  2. O-phosphoseryl-tRNA(Sec) selenium transferase (Sepsecs), O-phosphoseryl-tRNA(Sec) selenium transferase (Sepsecs)
This subpathway is part of the pathway selenocysteinyl-tRNA(Sec) biosynthesis, which is itself part of Aminoacyl-tRNA biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes selenocysteinyl-tRNA(Sec) from L-seryl-tRNA(Sec) (archaeal/eukaryal route), the pathway selenocysteinyl-tRNA(Sec) biosynthesis and in Aminoacyl-tRNA biosynthesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi25 – 32ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • kinase activity Source: MGI
  • seryl-selenocysteinyl-tRNA kinase activity Source: Reactome
  • tRNA binding Source: MGI

GO - Biological processi

  • selenocysteine incorporation Source: MGI
  • selenocysteinyl-tRNA(Sec) biosynthetic process Source: UniProtKB-UniPathway

Keywordsi

Molecular functionKinase, Transferase
Biological processProtein biosynthesis
LigandATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14957
BRENDAi2.7.1.164 3474
UniPathwayiUPA00906; UER00897

Names & Taxonomyi

Protein namesi
Recommended name:
L-seryl-tRNA(Sec) kinase (EC:2.7.1.164)
Alternative name(s):
O-phosphoseryl-tRNA(Sec) kinase
Gene namesi
Name:Pstk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2685945 Pstk

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000970811 – 359L-seryl-tRNA(Sec) kinaseAdd BLAST359

Proteomic databases

PaxDbiQ8BP74
PeptideAtlasiQ8BP74
PRIDEiQ8BP74

PTM databases

PhosphoSitePlusiQ8BP74

Expressioni

Gene expression databases

BgeeiENSMUSG00000063179
CleanExiMM_PSTK
ExpressionAtlasiQ8BP74 baseline and differential
GenevisibleiQ8BP74 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000075037

Structurei

3D structure databases

ProteinModelPortaliQ8BP74
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the L-seryl-tRNA(Sec) kinase family.Curated

Phylogenomic databases

eggNOGiKOG4622 Eukaryota
ENOG4111HI0 LUCA
GeneTreeiENSGT00390000017554
HOGENOMiHOG000115749
HOVERGENiHBG064780
InParanoidiQ8BP74
KOiK10837
OMAiISFFHYE
OrthoDBiEOG091G12WZ
PhylomeDBiQ8BP74
TreeFamiTF321264

Family and domain databases

InterProiView protein in InterPro
IPR013641 KTI12/PSTK
IPR020028 L-seryl-tRNA_Sec_kinase_euk
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF08433 KTI12, 1 hit
SUPFAMiSSF52540 SSF52540, 4 hits
TIGRFAMsiTIGR03575 selen_PSTK_euk, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BP74-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKTAAARGAT RRDGQPKLGL CVLCGLPAAG KSTFARALAL RLRRERGWAV
60 70 80 90 100
GVLSYDDVLP LALPDCDGTQ PRPSQWKMFR QELLKHLECF LVAVISGAQM
110 120 130 140 150
SAPPNRTEAV WEDFITCLKS QDLMIFPTAL EAQPCHLLAK PAVSRPLFLV
160 170 180 190 200
LDDNFYYQSM RYEVYQLARK YSLGFCQLFL DCPLETCLKR NGERSQPLPD
210 220 230 240 250
ETIQLMGRKI EKPNPEKNAW EHNSLIIQSS ACSLEASLEV TGLLLTALEN
260 270 280 290 300
PIKCVEDNTE QKETDRIICS TNILHKADET LRRTVSQTMR EAKDEQIPLN
310 320 330 340 350
NLKHLAEELN KLKADVLEDL RQGNRKYLCF QQTTDLSDII SSFCKERDTI

VQKYFSKQH
Note: No experimental confirmation available.
Length:359
Mass (Da):40,763
Last modified:May 1, 2007 - v2
Checksum:iE43BED6554DFFB2E
GO
Isoform 2 (identifier: Q8BP74-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     263-359: ETDRIICSTN...IVQKYFSKQH → NYLFY

Note: No experimental confirmation available.
Show »
Length:267
Mass (Da):30,072
Checksum:iF195D5CFBB13EBD9
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_024860263 – 359ETDRI…FSKQH → NYLFY in isoform 2. 1 PublicationAdd BLAST97

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK077585 mRNA Translation: BAC36878.1
BC110381 mRNA Translation: AAI10382.1
BC115503 mRNA Translation: AAI15504.2
CCDSiCCDS21914.1 [Q8BP74-1]
RefSeqiNP_001034623.1, NM_001039534.1 [Q8BP74-1]
UniGeneiMm.272948

Genome annotation databases

EnsembliENSMUST00000075610; ENSMUSP00000075037; ENSMUSG00000063179 [Q8BP74-1]
ENSMUST00000145114; ENSMUSP00000118636; ENSMUSG00000063179 [Q8BP74-2]
GeneIDi214580
KEGGimmu:214580
UCSCiuc009kbh.1 mouse [Q8BP74-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPSTK_MOUSE
AccessioniPrimary (citable) accession number: Q8BP74
Secondary accession number(s): Q0D2I7, Q14C09
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: May 1, 2007
Last modified: May 23, 2018
This is version 111 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health