UniProtKB - Q8BP47 (SYNC_MOUSE)
Protein
Asparagine--tRNA ligase, cytoplasmic
Gene
NARS1
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the attachment of asparagine to tRNA(Asn) in a two-step reaction: asparagine is first activated by ATP to form Asn-AMP and then transferred to the acceptor end of tRNA(Asn). In addition to its essential role in protein synthesis, acts as a signaling molecule that induces immune response in a CCR3-dependent manner.By similarity
Catalytic activityi
- EC:6.1.1.22By similarity
GO - Molecular functioni
- asparagine-tRNA ligase activity Source: UniProtKB
- ATP binding Source: UniProtKB-KW
- CCR3 chemokine receptor binding Source: UniProtKB
- nucleic acid binding Source: InterPro
- protein dimerization activity Source: UniProtKB
GO - Biological processi
- asparaginyl-tRNA aminoacylation Source: UniProtKB
- cell migration Source: UniProtKB
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Asparagine--tRNA ligase, cytoplasmic (EC:6.1.1.22By similarity)Alternative name(s): Asparaginyl-tRNA synthetase Short name: AsnRS Asparaginyl-tRNA synthetase 1 |
Gene namesi | Name:NARS1 Synonyms:Nars |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1917473, Nars |
Subcellular locationi
Other locations
- Cytoplasm By similarity
Cytosol
- cytosol Source: MGI
Mitochondrion
- mitochondrion Source: MGI
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000176497 | 1 – 559 | Asparagine--tRNA ligase, cytoplasmicAdd BLAST | 559 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 72 | PhosphoserineBy similarity | 1 | |
Modified residuei | 255 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 493 | PhosphoserineCombined sources | 1 | |
Modified residuei | 501 | N6-acetyllysineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q8BP47 |
jPOSTi | Q8BP47 |
MaxQBi | Q8BP47 |
PaxDbi | Q8BP47 |
PeptideAtlasi | Q8BP47 |
PRIDEi | Q8BP47 |
PTM databases
iPTMneti | Q8BP47 |
PhosphoSitePlusi | Q8BP47 |
SwissPalmi | Q8BP47 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000024587, Expressed in blood and 332 other tissues |
ExpressionAtlasi | Q8BP47, baseline and differential |
Genevisiblei | Q8BP47, MM |
Interactioni
Subunit structurei
Homodimer.
By similarityGO - Molecular functioni
- CCR3 chemokine receptor binding Source: UniProtKB
- protein dimerization activity Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 213922, 14 interactors |
IntActi | Q8BP47, 5 interactors |
MINTi | Q8BP47 |
STRINGi | 10090.ENSMUSP00000025483 |
Miscellaneous databases
RNActi | Q8BP47, protein |
Family & Domainsi
Domaini
The N-terminal domain (1-77) recruits and activates specific immune cells by interacting with CCR3-expressing cells.By similarity
Sequence similaritiesi
Belongs to the class-II aminoacyl-tRNA synthetase family.Curated
Phylogenomic databases
eggNOGi | KOG0555, Eukaryota |
GeneTreei | ENSGT01010000222368 |
HOGENOMi | CLU_004553_2_10_1 |
InParanoidi | Q8BP47 |
OMAi | HLAEYSH |
OrthoDBi | 1056670at2759 |
PhylomeDBi | Q8BP47 |
TreeFami | TF105664 |
Family and domain databases
InterProi | View protein in InterPro IPR004364, Aa-tRNA-synt_II IPR006195, aa-tRNA-synth_II IPR004522, Asn-tRNA-ligase IPR002312, Asp/Asn-tRNA-synth_IIb IPR012340, NA-bd_OB-fold IPR004365, NA-bd_OB_tRNA |
Pfami | View protein in Pfam PF00152, tRNA-synt_2, 1 hit PF01336, tRNA_anti-codon, 1 hit |
PRINTSi | PR01042, TRNASYNTHASP |
SUPFAMi | SSF50249, SSF50249, 1 hit |
TIGRFAMsi | TIGR00457, asnS, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 10 potential isoforms that are computationally mapped.Show allAlign All
Q8BP47-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSSEVIRGTA EMVLAELYVS DREGNDATGD GTKEKPFKTG LKALMTVGKE
60 70 80 90 100
PFPTIYVDSQ KENERWDVIS KSQMKNIKKM WHREQMKNDS REKKEAEDNL
110 120 130 140 150
RREKNLEEAK KIIIKNDPSL PEPACVKISA LEGYRGQRVK VFGWVHRLRR
160 170 180 190 200
QGKNLMFLVL RDGTGYLQCV LSDDLCQCYN GVVLSTESSV AVYGTLNLTP
210 220 230 240 250
KGKQAPGGHE LSCDFWELVG LAPAGGADNL INEESDVDVQ LNNRHMMIRG
260 270 280 290 300
ENMSKILKAR SMITRCFRDH FFDRGYCEVT TPTLVQTQVE GGATLFKLDY
310 320 330 340 350
FGEEAFLTQS SQLYLETCLP ALGDVFCIAQ SYRAEQSRTR RHLAEFTHVE
360 370 380 390 400
AECPFLTFED LLNRLEDLVC DVVDRVLKSP VASIVYELNP NFKPPKRPFR
410 420 430 440 450
RMNYSDAIEW LKEHDVKKED GTFYEFGDDI PEAPERLMTD TINEPILLCR
460 470 480 490 500
FPVEIKSFYM QRCPEDPRLT ESVDVLMPNV GEIVGGSMRS WDSEEILEGY
510 520 530 540 550
KREGIDPAPY YWYTDQRKYG TCPHGGYGLG LERFLSWILN RYHIRDVCLY
PRFLQRCRP
Computationally mapped potential isoform sequencesi
There are 10 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A498WGK2 | A0A498WGK2_MOUSE | Asparagine--tRNA ligase | Nars | 558 | Annotation score: | ||
A0A494BAX5 | A0A494BAX5_MOUSE | Asparagine--tRNA ligase | Nars | 497 | Annotation score: | ||
A0A494BB89 | A0A494BB89_MOUSE | Asparaginyl-tRNA synthetase | Nars | 517 | Annotation score: | ||
A0A494B996 | A0A494B996_MOUSE | Asparaginyl-tRNA synthetase | Nars | 127 | Annotation score: | ||
A0A494B927 | A0A494B927_MOUSE | Asparaginyl-tRNA synthetase | Nars | 210 | Annotation score: | ||
A0A494BAW6 | A0A494BAW6_MOUSE | Asparaginyl-tRNA synthetase | Nars | 164 | Annotation score: | ||
A0A494B949 | A0A494B949_MOUSE | Asparaginyl-tRNA synthetase | Nars | 70 | Annotation score: | ||
A0A494B9G0 | A0A494B9G0_MOUSE | Asparaginyl-tRNA synthetase | Nars | 69 | Annotation score: | ||
A0A494BB92 | A0A494BB92_MOUSE | Asparaginyl-tRNA synthetase | Nars | 43 | Annotation score: | ||
A0A494BB81 | A0A494BB81_MOUSE | Asparaginyl-tRNA synthetase | Nars | 35 | Annotation score: |
Sequence cautioni
The sequence AAH52849 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB29032 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 15 | Missing in BAB29032 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 82 | H → R in BAB29032 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 309 | Q → E in BAB29032 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 469 | L → P in BAE43117 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 526 | G → E in AAH52849 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK013880 mRNA Translation: BAB29032.1 Different initiation. AK077699 mRNA Translation: BAC36965.1 AK172657 mRNA Translation: BAE43117.1 BC052849 mRNA Translation: AAH52849.1 Different initiation. |
RefSeqi | NP_001136422.1, NM_001142950.1 NP_081626.2, NM_027350.3 |
Genome annotation databases
Ensembli | ENSMUST00000237400; ENSMUSP00000157402; ENSMUSG00000024587 |
GeneIDi | 70223 |
KEGGi | mmu:70223 |
UCSCi | uc008fek.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK013880 mRNA Translation: BAB29032.1 Different initiation. AK077699 mRNA Translation: BAC36965.1 AK172657 mRNA Translation: BAE43117.1 BC052849 mRNA Translation: AAH52849.1 Different initiation. |
RefSeqi | NP_001136422.1, NM_001142950.1 NP_081626.2, NM_027350.3 |
3D structure databases
SMRi | Q8BP47 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 213922, 14 interactors |
IntActi | Q8BP47, 5 interactors |
MINTi | Q8BP47 |
STRINGi | 10090.ENSMUSP00000025483 |
PTM databases
iPTMneti | Q8BP47 |
PhosphoSitePlusi | Q8BP47 |
SwissPalmi | Q8BP47 |
Proteomic databases
EPDi | Q8BP47 |
jPOSTi | Q8BP47 |
MaxQBi | Q8BP47 |
PaxDbi | Q8BP47 |
PeptideAtlasi | Q8BP47 |
PRIDEi | Q8BP47 |
Protocols and materials databases
Antibodypediai | 9706, 179 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000237400; ENSMUSP00000157402; ENSMUSG00000024587 |
GeneIDi | 70223 |
KEGGi | mmu:70223 |
UCSCi | uc008fek.2, mouse |
Organism-specific databases
CTDi | 70223 |
MGIi | MGI:1917473, Nars |
Phylogenomic databases
eggNOGi | KOG0555, Eukaryota |
GeneTreei | ENSGT01010000222368 |
HOGENOMi | CLU_004553_2_10_1 |
InParanoidi | Q8BP47 |
OMAi | HLAEYSH |
OrthoDBi | 1056670at2759 |
PhylomeDBi | Q8BP47 |
TreeFami | TF105664 |
Miscellaneous databases
BioGRID-ORCSi | 70223, 9 hits in 18 CRISPR screens |
ChiTaRSi | Nars, mouse |
PROi | PR:Q8BP47 |
RNActi | Q8BP47, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000024587, Expressed in blood and 332 other tissues |
ExpressionAtlasi | Q8BP47, baseline and differential |
Genevisiblei | Q8BP47, MM |
Family and domain databases
InterProi | View protein in InterPro IPR004364, Aa-tRNA-synt_II IPR006195, aa-tRNA-synth_II IPR004522, Asn-tRNA-ligase IPR002312, Asp/Asn-tRNA-synth_IIb IPR012340, NA-bd_OB-fold IPR004365, NA-bd_OB_tRNA |
Pfami | View protein in Pfam PF00152, tRNA-synt_2, 1 hit PF01336, tRNA_anti-codon, 1 hit |
PRINTSi | PR01042, TRNASYNTHASP |
SUPFAMi | SSF50249, SSF50249, 1 hit |
TIGRFAMsi | TIGR00457, asnS, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SYNC_MOUSE | |
Accessioni | Q8BP47Primary (citable) accession number: Q8BP47 Secondary accession number(s): Q3T9A7, Q7TPX0, Q9CRY5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 25, 2003 |
Last sequence update: | July 1, 2008 | |
Last modified: | December 2, 2020 | |
This is version 140 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries