UniProtKB - Q8BN78 (KAISO_MOUSE)
Transcriptional regulator Kaiso
Zbtb33
Functioni
Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3' also known as the consensus kaiso binding site (KBS). May recruit the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. Contributes to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2. Represses expression of MMP7 in conjunction with transcriptional corepressors CBFA2T3, CBFA2T2 and RUNX1T1 (By similarity).
By similarity4 PublicationsRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 492 – 514 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 520 – 542 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 548 – 571 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 24 |
GO - Molecular functioni
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- methyl-CpG binding Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
GO - Biological processi
- negative regulation of transcription, DNA-templated Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: GO_Central
- transcription, DNA-templated Source: MGI
- Wnt signaling pathway Source: UniProtKB-KW
Keywordsi
Molecular function | Activator, DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation, Wnt signaling pathway |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Transcriptional regulator KaisoAlternative name(s): Zinc finger and BTB domain-containing protein 33 |
Gene namesi | Name:Zbtb33 Synonyms:Kaiso |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1927290, Zbtb33 |
VEuPathDBi | HostDB:ENSMUSG00000048047 |
Subcellular locationi
Nucleus
- Nucleus 3 Publications
Cytosol
- cytosol Source: MGI
Nucleus
- nucleolus Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: MGI
Plasma Membrane
- plasma membrane Source: MGI
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 472 | K → A: Abrogates nuclear localization. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000046989 | 1 – 671 | Transcriptional regulator KaisoAdd BLAST | 671 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 151 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 153 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 251 | PhosphothreonineBy similarity | 1 | |
Cross-linki | 388 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 405 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 412 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 447 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 463 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 472 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 477 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 537 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 568 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 580 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 609 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 616 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q8BN78 |
MaxQBi | Q8BN78 |
PaxDbi | Q8BN78 |
PRIDEi | Q8BN78 |
ProteomicsDBi | 263572 |
PTM databases
iPTMneti | Q8BN78 |
PhosphoSitePlusi | Q8BN78 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000048047, Expressed in pineal body and 265 other tissues |
ExpressionAtlasi | Q8BN78, baseline and differential |
Genevisiblei | Q8BN78, MM |
Interactioni
Subunit structurei
Interacts with NCOR1 (By similarity). Self-associates.
Interacts with CTNND1, and this interaction inhibits binding to both methylated and non-methylated DNA.
Interacts with CTNND2.
Interacts with KPNA2/RCH1, which may mediate nuclear import of this protein.
Interacts with CBFA2T3 (By similarity).
By similarity4 PublicationsBinary interactionsi
Q8BN78
With | #Exp. | IntAct |
---|---|---|
Ctnnd1 [P30999] | 3 | EBI-1216314,EBI-529924 |
Protein-protein interaction databases
BioGRIDi | 208181, 7 interactors |
IntActi | Q8BN78, 3 interactors |
MINTi | Q8BN78 |
STRINGi | 10090.ENSMUSP00000110795 |
Miscellaneous databases
RNActi | Q8BN78, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 32 – 94 | BTBPROSITE-ProRule annotationAdd BLAST | 63 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 136 | Self-associationAdd BLAST | 136 | |
Regioni | 1 – 103 | Interaction with NCOR1By similarityAdd BLAST | 103 | |
Regioni | 127 – 161 | DisorderedSequence analysisAdd BLAST | 35 | |
Regioni | 298 – 571 | Interaction with CBFA2T3By similarityAdd BLAST | 274 | |
Regioni | 332 – 365 | DisorderedSequence analysisAdd BLAST | 34 | |
Regioni | 451 – 474 | DisorderedSequence analysisAdd BLAST | 24 | |
Regioni | 452 – 671 | Interaction with CTNND1Add BLAST | 220 | |
Regioni | 512 – 637 | Required for DNA-bindingAdd BLAST | 126 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 469 – 478 | Nuclear localization signal1 Publication | 10 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 127 – 145 | Polar residuesSequence analysisAdd BLAST | 19 | |
Compositional biasi | 342 – 365 | Polar residuesSequence analysisAdd BLAST | 24 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 492 – 514 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 520 – 542 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 548 – 571 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 24 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000157481 |
InParanoidi | Q8BN78 |
OMAi | TELEDHY |
OrthoDBi | 567120at2759 |
PhylomeDBi | Q8BN78 |
TreeFami | TF333100 |
Family and domain databases
Gene3Di | 3.30.710.10, 1 hit |
InterProi | View protein in InterPro IPR000210, BTB/POZ_dom IPR011333, SKP1/BTB/POZ_sf IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00651, BTB, 1 hit |
SMARTi | View protein in SMART SM00225, BTB, 1 hit SM00355, ZnF_C2H2, 3 hits |
SUPFAMi | SSF54695, SSF54695, 1 hit SSF57667, SSF57667, 2 hits |
PROSITEi | View protein in PROSITE PS50097, BTB, 1 hit PS00028, ZINC_FINGER_C2H2_1, 3 hits PS50157, ZINC_FINGER_C2H2_2, 3 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MESRKLISAT DIQYSASLLN SLNEQRGHGL FCDVTVIVED RKFRAHRNIL
60 70 80 90 100
SASSTYFHQL FSVAGQVVEL SFIRAEIFAE ILNYIYSSKV VRVRADLLDE
110 120 130 140 150
LIKSGQLLGV KFIAELGVPL SQVKSISGTE QDGTAETLPS SSSDKSLDME
160 170 180 190 200
KSKDEAQDNG ATVMPIITES FSLSAEDNEM KKIIVTDSDD DDDDDVIFCS
210 220 230 240 250
EILPAKEDLP SNNTATQVQP NPASVAISEV TPCASNNSPP VTNITPTQLP
260 270 280 290 300
TPVNQATLSQ TQGSEELLVS SASTHLTPNI ILLNQAPLTA PPSASSSLPN
310 320 330 340 350
HMSSSVNVLV QNQQTPNSAV LTGNKAEEEE EIIDDDDDII SSSPDSAVSN
360 370 380 390 400
TSLVPQADNS KSTTLDGSLT QKMQIPVLPQ EPPSNSLKIS DVITRNTNDP
410 420 430 440 450
GLRSKHVMEG QKIITLDTAT EIEGLSTGCK VYANIGEDTY DIVIPVKDDP
460 470 480 490 500
DGGEAKLDNE LPKTSGSEPP NKRMKVKHDD HYELIVDGRV YYICIVCKRS
510 520 530 540 550
YVCLTSLRRH FNIHSWEKKY QCRYCDKVFP LAEYRTKHEI HHTGERRYQC
560 570 580 590 600
LTCGKSFINY QFMSSHIKSV HSQDPSGDSK LYRLHPCKSL QIRQYAYLSN
610 620 630 640 650
KSSAMPVMKD DAVGYKVDAG KEPPVGTTST PPQNKSTFWE DIFIQQENDS
660 670
IFKQNVTDGS TEFEFIIPES Y
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA2A3Z2 | A2A3Z2_MOUSE | Transcriptional regulator Kaiso | Zbtb33 | 617 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 441 | D → N in AAD20989 (PubMed:10207085).Curated | 1 | |
Sequence conflicti | 451 | D → H in AAD20989 (PubMed:10207085).Curated | 1 | |
Sequence conflicti | 640 | E → K in AAD20989 (PubMed:10207085).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF097416 mRNA Translation: AAD20989.1 AK087417 mRNA Translation: BAC39866.1 AK089423 mRNA Translation: BAC40875.1 |
CCDSi | CCDS30088.1 |
RefSeqi | NP_001072981.1, NM_001079513.1 NP_064652.2, NM_020256.2 |
Genome annotation databases
Ensembli | ENSMUST00000049740; ENSMUSP00000049983; ENSMUSG00000048047 ENSMUST00000115142; ENSMUSP00000110795; ENSMUSG00000048047 |
GeneIDi | 56805 |
KEGGi | mmu:56805 |
UCSCi | uc009szp.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF097416 mRNA Translation: AAD20989.1 AK087417 mRNA Translation: BAC39866.1 AK089423 mRNA Translation: BAC40875.1 |
CCDSi | CCDS30088.1 |
RefSeqi | NP_001072981.1, NM_001079513.1 NP_064652.2, NM_020256.2 |
3D structure databases
AlphaFoldDBi | Q8BN78 |
SMRi | Q8BN78 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 208181, 7 interactors |
IntActi | Q8BN78, 3 interactors |
MINTi | Q8BN78 |
STRINGi | 10090.ENSMUSP00000110795 |
PTM databases
iPTMneti | Q8BN78 |
PhosphoSitePlusi | Q8BN78 |
Proteomic databases
EPDi | Q8BN78 |
MaxQBi | Q8BN78 |
PaxDbi | Q8BN78 |
PRIDEi | Q8BN78 |
ProteomicsDBi | 263572 |
Protocols and materials databases
Antibodypediai | 401, 209 antibodies from 31 providers |
DNASUi | 56805 |
Genome annotation databases
Ensembli | ENSMUST00000049740; ENSMUSP00000049983; ENSMUSG00000048047 ENSMUST00000115142; ENSMUSP00000110795; ENSMUSG00000048047 |
GeneIDi | 56805 |
KEGGi | mmu:56805 |
UCSCi | uc009szp.1, mouse |
Organism-specific databases
CTDi | 10009 |
MGIi | MGI:1927290, Zbtb33 |
VEuPathDBi | HostDB:ENSMUSG00000048047 |
Phylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000157481 |
InParanoidi | Q8BN78 |
OMAi | TELEDHY |
OrthoDBi | 567120at2759 |
PhylomeDBi | Q8BN78 |
TreeFami | TF333100 |
Miscellaneous databases
BioGRID-ORCSi | 56805, 0 hits in 74 CRISPR screens |
ChiTaRSi | Zbtb33, mouse |
PROi | PR:Q8BN78 |
RNActi | Q8BN78, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000048047, Expressed in pineal body and 265 other tissues |
ExpressionAtlasi | Q8BN78, baseline and differential |
Genevisiblei | Q8BN78, MM |
Family and domain databases
Gene3Di | 3.30.710.10, 1 hit |
InterProi | View protein in InterPro IPR000210, BTB/POZ_dom IPR011333, SKP1/BTB/POZ_sf IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00651, BTB, 1 hit |
SMARTi | View protein in SMART SM00225, BTB, 1 hit SM00355, ZnF_C2H2, 3 hits |
SUPFAMi | SSF54695, SSF54695, 1 hit SSF57667, SSF57667, 2 hits |
PROSITEi | View protein in PROSITE PS50097, BTB, 1 hit PS00028, ZINC_FINGER_C2H2_1, 3 hits PS50157, ZINC_FINGER_C2H2_2, 3 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | KAISO_MOUSE | |
Accessioni | Q8BN78Primary (citable) accession number: Q8BN78 Secondary accession number(s): Q8C226, Q9WTZ7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | March 1, 2003 | |
Last modified: | May 25, 2022 | |
This is version 148 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot