Zbtb33

Functionⁱ

Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3' also known as the consensus kaiso binding site (KBS). May recruit the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. Contributes to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2. Represses expression of MMP7 in conjunction with transcriptional corepressors CBFA2T3, CBFA2T2 and RUNX1T1 (By similarity).By similarity4 Publications

Regions

Feature key	Position(s)	DescriptionActions	Length
Zinc fingerⁱ	492 – 514	C2H2-type 1PROSITE-ProRule annotationAdd BLAST	23
Zinc fingerⁱ	520 – 542	C2H2-type 2PROSITE-ProRule annotationAdd BLAST	23
Zinc fingerⁱ	548 – 571	C2H2-type 3PROSITE-ProRule annotationAdd BLAST	24

GO - Molecular functionⁱ

metal ion binding Source: UniProtKB-KW
methyl-CpG binding Source: MGI
sequence-specific DNA binding Source: MGI

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

negative regulation of transcription, DNA-templated Source: UniProtKB
transcription, DNA-templated
Source: MGI
Inferred from sequence alignmentⁱ
- 10207085
Wnt signaling pathway Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Activator, DNA-binding, Repressor
Biological process	Transcription, Transcription regulation, Wnt signaling pathway
Ligand	Metal-binding, Zinc

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Transcriptional regulator Kaiso Alternative name(s): Zinc finger and BTB domain-containing protein 33
Gene namesⁱ	Name:Zbtb33 Synonyms:Kaiso
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome X

Organism-specific databases

MGIⁱ	MGI:1927290 Zbtb33

Keywords - Cellular componentⁱ

Nucleus

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	472	K → A: Abrogates nuclear localization. 1 Publication		1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000046989	1 – 671	Transcriptional regulator KaisoAdd BLAST		671

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Cross-linkⁱ	151	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	153	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residueⁱ	251	PhosphothreonineBy similarity	1
Cross-linkⁱ	388	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	405	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	412	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	447	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	463	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	472	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	477	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	537	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	568	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	580	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	609	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	616	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMⁱ

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDⁱ	Q8BN78
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q8BN78
PaxDbⁱ	Q8BN78
PRIDEⁱ	Q8BN78

PTM databases

iPTMnetⁱ	Q8BN78
PhosphoSitePlusⁱ	Q8BN78

Expressionⁱ

Tissue specificityⁱ

Expressed in brain, heart, kidney, liver, lung, neuromuscular junctions, skeletal muscle, spleen and testis.2 Publications

Gene expression databases

Bgeeⁱ	ENSMUSG00000048047 Expressed in 240 organ(s), highest expression level in pineal body
CleanExⁱ	MM_ZBTB33
ExpressionAtlasⁱ	Q8BN78 baseline and differential
Genevisibleⁱ	Q8BN78 MM

Interactionⁱ

Subunit structureⁱ

Interacts with NCOR1 (By similarity). Self-associates. Interacts with CTNND1, and this interaction inhibits binding to both methylated and non-methylated DNA. Interacts with CTNND2. Interacts with KPNA2/RCH1, which may mediate nuclear import of this protein. Interacts with CBFA2T3 (By similarity).By similarity4 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
Ctnnd1	P30999	3	EBI-1216314,EBI-529924

With

Entry

#Exp.

IntAct

Notes

Ctnnd1

P30999

3

EBI-1216314,EBI-529924

Protein-protein interaction databases

BioGridⁱ	208181, 7 interactors
IntActⁱ	Q8BN78, 2 interactors
STRINGⁱ	10090.ENSMUSP00000049983

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q8BN78
SMRⁱ	Q8BN78
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	32 – 94	BTBPROSITE-ProRule annotationAdd BLAST		63

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	1 – 136	Self-associationAdd BLAST	136
Regionⁱ	1 – 103	Interaction with NCOR1By similarityAdd BLAST	103
Regionⁱ	298 – 571	Interaction with CBFA2T3By similarityAdd BLAST	274
Regionⁱ	452 – 671	Interaction with CTNND1Add BLAST	220
Regionⁱ	512 – 637	Required for DNA-bindingAdd BLAST	126

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	469 – 478	Nuclear localization signal1 Publication		10

Zinc finger

Feature key	Position(s)	DescriptionActions	Length
Zinc fingerⁱ	492 – 514	C2H2-type 1PROSITE-ProRule annotationAdd BLAST	23
Zinc fingerⁱ	520 – 542	C2H2-type 2PROSITE-ProRule annotationAdd BLAST	23
Zinc fingerⁱ	548 – 571	C2H2-type 3PROSITE-ProRule annotationAdd BLAST	24

Keywords - Domainⁱ

Repeat, Zinc-finger

Phylogenomic databases

eggNOGⁱ	KOG1721 Eukaryota COG5048 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00810000125416
HOGENOMⁱ	HOG000230932
HOVERGENⁱ	HBG079575
InParanoidⁱ	Q8BN78
KEGG Orthology (KO) More...KOⁱ	K10507
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G02SP
PhylomeDBⁱ	Q8BN78
TreeFamⁱ	TF333100

Family and domain databases

InterProⁱ	View protein in InterPro IPR000210 BTB/POZ_dom IPR011333 SKP1/BTB/POZ_sf IPR036236 Znf_C2H2_sf IPR013087 Znf_C2H2_type
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00651 BTB, 1 hit
SMARTⁱ	View protein in SMART SM00225 BTB, 1 hit SM00355 ZnF_C2H2, 3 hits
SUPFAMⁱ	SSF54695 SSF54695, 1 hit SSF57667 SSF57667, 2 hits
PROSITEⁱ	View protein in PROSITE PS50097 BTB, 1 hit PS00028 ZINC_FINGER_C2H2_1, 3 hits PS50157 ZINC_FINGER_C2H2_2, 3 hits

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show all Align All

Q8BN78-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MESRKLISAT DIQYSASLLN SLNEQRGHGL FCDVTVIVED RKFRAHRNIL 
        60         70         80         90        100
SASSTYFHQL FSVAGQVVEL SFIRAEIFAE ILNYIYSSKV VRVRADLLDE 
       110        120        130        140        150
LIKSGQLLGV KFIAELGVPL SQVKSISGTE QDGTAETLPS SSSDKSLDME 
       160        170        180        190        200
KSKDEAQDNG ATVMPIITES FSLSAEDNEM KKIIVTDSDD DDDDDVIFCS 
       210        220        230        240        250
EILPAKEDLP SNNTATQVQP NPASVAISEV TPCASNNSPP VTNITPTQLP 
       260        270        280        290        300
TPVNQATLSQ TQGSEELLVS SASTHLTPNI ILLNQAPLTA PPSASSSLPN 
       310        320        330        340        350
HMSSSVNVLV QNQQTPNSAV LTGNKAEEEE EIIDDDDDII SSSPDSAVSN 
       360        370        380        390        400
TSLVPQADNS KSTTLDGSLT QKMQIPVLPQ EPPSNSLKIS DVITRNTNDP 
       410        420        430        440        450
GLRSKHVMEG QKIITLDTAT EIEGLSTGCK VYANIGEDTY DIVIPVKDDP 
       460        470        480        490        500
DGGEAKLDNE LPKTSGSEPP NKRMKVKHDD HYELIVDGRV YYICIVCKRS 
       510        520        530        540        550
YVCLTSLRRH FNIHSWEKKY QCRYCDKVFP LAEYRTKHEI HHTGERRYQC 
       560        570        580        590        600
LTCGKSFINY QFMSSHIKSV HSQDPSGDSK LYRLHPCKSL QIRQYAYLSN 
       610        620        630        640        650
KSSAMPVMKD DAVGYKVDAG KEPPVGTTST PPQNKSTFWE DIFIQQENDS 
       660        670 
IFKQNVTDGS TEFEFIIPES Y

Length:671

Mass (Da):74,050

Last modified:March 1, 2003 - v1

Checksum:ⁱCFD23E0C15B491D1

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	A2A3Z2	A2A3Z2_MOUSE		Transcriptional regulator Kaiso Transcriptional regulator Kaiso	Zbtb33	617	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	441	D → N in AAD20989 (PubMed:10207085).Curated	1
Sequence conflictⁱ	451	D → H in AAD20989 (PubMed:10207085).Curated	1
Sequence conflictⁱ	640	E → K in AAD20989 (PubMed:10207085).Curated	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF097416 mRNA Translation: AAD20989.1 AK087417 mRNA Translation: BAC39866.1 AK089423 mRNA Translation: BAC40875.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS30088.1
NCBI Reference Sequences More...RefSeqⁱ	NP_001072981.1, NM_001079513.1 NP_064652.2, NM_020256.2
UniGeneⁱ	Mm.391248

Genome annotation databases

Ensemblⁱ	ENSMUST00000049740; ENSMUSP00000049983; ENSMUSG00000048047 ENSMUST00000115142; ENSMUSP00000110795; ENSMUSG00000048047
GeneIDⁱ	56805
KEGGⁱ	mmu:56805
UCSC genome browser More...UCSCⁱ	uc009szp.1 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q8BN78	Zinc finger and BTB domain containing 33		MOUSE		671	UniRef100_Q8BN78
Transcriptional regulator Kaiso (Fragment)		MOUSE		617

Protein	Similar proteins		Species	Score	Length	Source
Q8BN78	Zinc finger and BTB domain containing 33		MOUSE		671	UniRef90_Q8BN78
transcriptional regulator Kaiso		MESAU		683
Transcriptional regulator Kaiso		CRIGR		682
UPI00038BF94C		MICOH		681
UPI000A3191E6		MUSCR		672
+5

Protein	Similar proteins		Species	Score	Length	Source
Q8BN78	Transcriptional regulator Kaiso		XENLA		701	UniRef50_Q8UVQ4
Uncharacterized protein		LATCH		684
Transcriptional regulator Kaiso (Fragment)		OPHHA		677
Transcriptional regulator Kaiso		EGRGA		678
Transcriptional regulator Kaiso		APAVI		680
+99

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF097416 mRNA Translation: AAD20989.1 AK087417 mRNA Translation: BAC39866.1 AK089423 mRNA Translation: BAC40875.1
CCDSⁱ	CCDS30088.1
RefSeqⁱ	NP_001072981.1, NM_001079513.1 NP_064652.2, NM_020256.2
UniGeneⁱ	Mm.391248

3D structure databases

ProteinModelPortalⁱ	Q8BN78
SMRⁱ	Q8BN78
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	208181, 7 interactors
IntActⁱ	Q8BN78, 2 interactors
STRINGⁱ	10090.ENSMUSP00000049983

PTM databases

iPTMnetⁱ	Q8BN78
PhosphoSitePlusⁱ	Q8BN78

Proteomic databases

EPDⁱ	Q8BN78
MaxQBⁱ	Q8BN78
PaxDbⁱ	Q8BN78
PRIDEⁱ	Q8BN78

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000049740; ENSMUSP00000049983; ENSMUSG00000048047 ENSMUST00000115142; ENSMUSP00000110795; ENSMUSG00000048047
GeneIDⁱ	56805
KEGGⁱ	mmu:56805
UCSCⁱ	uc009szp.1 mouse

Organism-specific databases

CTDⁱ	10009
MGIⁱ	MGI:1927290 Zbtb33

Phylogenomic databases

eggNOGⁱ	KOG1721 Eukaryota COG5048 LUCA
GeneTreeⁱ	ENSGT00810000125416
HOGENOMⁱ	HOG000230932
HOVERGENⁱ	HBG079575
InParanoidⁱ	Q8BN78
KOⁱ	K10507
OrthoDBⁱ	EOG091G02SP
PhylomeDBⁱ	Q8BN78
TreeFamⁱ	TF333100

Miscellaneous databases

ChiTaRSⁱ	Zbtb33 mouse
Protein Ontology More...PROⁱ	PR:Q8BN78
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000048047 Expressed in 240 organ(s), highest expression level in pineal body
CleanExⁱ	MM_ZBTB33
ExpressionAtlasⁱ	Q8BN78 baseline and differential
Genevisibleⁱ	Q8BN78 MM

Family and domain databases

InterProⁱ	View protein in InterPro IPR000210 BTB/POZ_dom IPR011333 SKP1/BTB/POZ_sf IPR036236 Znf_C2H2_sf IPR013087 Znf_C2H2_type
Pfamⁱ	View protein in Pfam PF00651 BTB, 1 hit
SMARTⁱ	View protein in SMART SM00225 BTB, 1 hit SM00355 ZnF_C2H2, 3 hits
SUPFAMⁱ	SSF54695 SSF54695, 1 hit SSF57667 SSF57667, 2 hits
PROSITEⁱ	View protein in PROSITE PS50097 BTB, 1 hit PS00028 ZINC_FINGER_C2H2_1, 3 hits PS50157 ZINC_FINGER_C2H2_2, 3 hits
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	KAISO_MOUSE
Accessionⁱ	Q8BN78Primary (citable) accession number: Q8BN78 Secondary accession number(s): Q8C226, Q9WTZ7
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
	Last sequence update:	March 1, 2003
	Last modified:	November 7, 2018
	This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q8BN78 (KAISO_MOUSE)

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Transcriptional regulator Kaiso

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Cytosol

Nucleus

Plasma Membrane

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Motif

Zinc finger

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ