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Protein

Serine/threonine-protein kinase VRK2

Gene

Vrk2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine/threonine kinase that regulates several signal transduction pathways. Isoform 1 modulates the stress response to hypoxia and cytokines, such as interleukin-1 beta (IL1B) and this is dependent on its interaction with MAPK8IP1, which assembles mitogen-activated protein kinase (MAPK) complexes. Inhibition of signal transmission mediated by the assembly of MAPK8IP1-MAPK complexes reduces JNK phosphorylation and JUN-dependent transcription. Phosphorylates histone H3. Phosphorylates 'Thr-18' of p53/TP53, and thereby increases its stability and activity. Phosphorylates BANF1 and disrupts its ability to bind DNA and reduces its binding to LEM domain-containing proteins. Downregulates the transactivation of transcription induced by ERBB2, HRAS, BRAF, and MEK1. Blocks the phosphorylation of ERK in response to ERBB2 and HRAS. May also phosphorylate MAPK8IP1. Can also phosphorylate the following substrates that are commonly used to establish in vitro kinase activity: casein, MBP and histone H2B, but it is not sure that this is physiologically relevant (By similarity).By similarity1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei61ATPPROSITE-ProRule annotation1
Active sitei166Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi35 – 43ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-2995383 Initiation of Nuclear Envelope Reformation

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase VRK2 (EC:2.7.11.1)
Alternative name(s):
Vaccinia-related kinase 2
Gene namesi
Name:Vrk2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1917172 Vrk2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei482 – 502Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000868071 – 503Serine/threonine-protein kinase VRK2Add BLAST503

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei336PhosphothreonineBy similarity1

Post-translational modificationi

Autophosphorylated.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8BN21
PeptideAtlasiQ8BN21
PRIDEiQ8BN21

PTM databases

iPTMnetiQ8BN21
PhosphoSitePlusiQ8BN21

Expressioni

Tissue specificityi

Expressed in liver, kidney and muscle. Weakly expressed in thymus, bone marrow and spleen.1 Publication

Developmental stagei

Weakly expressed in embryo compared to VRK1 and VRK3. Expressed from E10.5 to E14 in developing liver and then decreases. It increases again from E17.5 and remains thereafter. Highly expressed in hematopoietic embryonic tissues from E10.5 to E14.5. Weakly expressed in the yolk-sac.1 Publication

Gene expression databases

BgeeiENSMUSG00000064090 Expressed in 224 organ(s), highest expression level in blood
CleanExiMM_VRK2
ExpressionAtlasiQ8BN21 baseline and differential
GenevisibleiQ8BN21 MM

Interactioni

Subunit structurei

Interacts with MAP3K7, MAP2K7, MAP2K1, KSR1, RAN and MAPK8IP1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi213758, 2 interactors
STRINGi10090.ENSMUSP00000077471

Structurei

3D structure databases

ProteinModelPortaliQ8BN21
SMRiQ8BN21
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 313Protein kinasePROSITE-ProRule annotationAdd BLAST285

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni392 – 503Interaction with MAP3K7By similarityAdd BLAST112

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1164 Eukaryota
ENOG410XPGP LUCA
GeneTreeiENSGT00920000148988
HOVERGENiHBG007532
InParanoidiQ8BN21
KOiK08816
OMAiSSCCEIA
OrthoDBiEOG091G05V5
PhylomeDBiQ8BN21
TreeFamiTF106473

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 1 potential isoform that is computationally mapped.iShow all

Isoform 1 (identifier: Q8BN21-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAPRRKEKYK LPVPLPEGKI LDDMEGNRWA LGKMIGSGGF GLIYLAFPTN
60 70 80 90 100
KPNKDARHVI KLEYQENGPL FSELKFYQRA AKRECIQKWI QQRKLDYLGI
110 120 130 140 150
PVFYGFGLTD FKGRSYRFMV MERLGIDLQK LLDQNGGFKK LTVLQLGIRM
160 170 180 190 200
LDVLEYIHEN EYVHGDIKAA NLLLDFTNPD RVYLADYGLS YRYCPNGNHK
210 220 230 240 250
QYQEDPRKGH NGTIEFTSLD AHKGVAPSRR SDVEILGYCM LHWLFGKLPW
260 270 280 290 300
EAKLDDPVAV QTAKTNLLDE LPESVLKWAP SGSSCSELVK YLMYVHNLAY
310 320 330 340 350
DDKPDYQKLK KILNPDGVPL GPLEFSTKVQ SVHVRTPAQQ KVDSPKATRK
360 370 380 390 400
PANEFPAKFP KKVHRETRAR QREEQEDSQP TMLQSRPAAP ENSRTRKIHE
410 420 430 440 450
YSDIFSEMQS LQQTPSYMSF QGSYCKPYLD CTRRDPIRKP RSLPRYRHTP
460 470 480 490 500
TGNLGVTDLE SSPRFWPAIF QLTLSEETKA DVYYYGITIF CLLIFVFLAL

YFL
Length:503
Mass (Da):58,119
Last modified:October 10, 2003 - v2
Checksum:i6F29E3E412ECB221
GO
Isoform 2 (identifier: Q8BN21-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     342-390: Missing.

Note: No experimental confirmation available.
Show »
Length:454
Mass (Da):52,493
Checksum:i4AAD5406E8214578
GO
Isoform 3 (identifier: Q8BN21-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     151-163: LDVLEYIHENEYV → VSLRDLTGDLLDI
     164-503: Missing.

Note: No experimental confirmation available.
Show »
Length:163
Mass (Da):18,844
Checksum:i43B728DB7DC2FE52
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q5SP86Q5SP86_MOUSE
Serine/threonine-protein kinase VRK...
Vrk2
111Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti33K → R in AAN64922 (PubMed:12417526).Curated1
Sequence conflicti226A → P in BAB28393 (PubMed:16141072).Curated1
Sequence conflicti319P → L in BAE32620 (PubMed:16141072).Curated1
Sequence conflicti319P → L in AAH13520 (PubMed:15489334).Curated1
Sequence conflicti333H → R in BAE32620 (PubMed:16141072).Curated1
Sequence conflicti333H → R in AAH13520 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_008541151 – 163LDVLE…ENEYV → VSLRDLTGDLLDI in isoform 3. 1 PublicationAdd BLAST13
Alternative sequenceiVSP_008542164 – 503Missing in isoform 3. 1 PublicationAdd BLAST340
Alternative sequenceiVSP_008543342 – 390Missing in isoform 2. 1 PublicationAdd BLAST49

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF513620 mRNA Translation: AAN64922.1
AK012664 mRNA Translation: BAB28393.1
AK053297 mRNA Translation: BAC35335.1
AK154485 mRNA Translation: BAE32620.1
AL929272, AC083815 Genomic DNA Translation: CAI52021.1
BC013520 mRNA Translation: AAH13520.1
CCDSiCCDS24486.1 [Q8BN21-1]
RefSeqiNP_001239376.1, NM_001252447.1 [Q8BN21-2]
NP_081536.2, NM_027260.3 [Q8BN21-1]
XP_006514864.1, XM_006514801.3 [Q8BN21-1]
UniGeneiMm.285351

Genome annotation databases

EnsembliENSMUST00000078362; ENSMUSP00000077471; ENSMUSG00000064090 [Q8BN21-1]
ENSMUST00000109504; ENSMUSP00000105130; ENSMUSG00000064090 [Q8BN21-1]
GeneIDi69922
KEGGimmu:69922
UCSCiuc007igf.2 mouse [Q8BN21-1]
uc007igg.2 mouse [Q8BN21-3]
uc011xsk.2 mouse [Q8BN21-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF513620 mRNA Translation: AAN64922.1
AK012664 mRNA Translation: BAB28393.1
AK053297 mRNA Translation: BAC35335.1
AK154485 mRNA Translation: BAE32620.1
AL929272, AC083815 Genomic DNA Translation: CAI52021.1
BC013520 mRNA Translation: AAH13520.1
CCDSiCCDS24486.1 [Q8BN21-1]
RefSeqiNP_001239376.1, NM_001252447.1 [Q8BN21-2]
NP_081536.2, NM_027260.3 [Q8BN21-1]
XP_006514864.1, XM_006514801.3 [Q8BN21-1]
UniGeneiMm.285351

3D structure databases

ProteinModelPortaliQ8BN21
SMRiQ8BN21
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi213758, 2 interactors
STRINGi10090.ENSMUSP00000077471

PTM databases

iPTMnetiQ8BN21
PhosphoSitePlusiQ8BN21

Proteomic databases

PaxDbiQ8BN21
PeptideAtlasiQ8BN21
PRIDEiQ8BN21

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000078362; ENSMUSP00000077471; ENSMUSG00000064090 [Q8BN21-1]
ENSMUST00000109504; ENSMUSP00000105130; ENSMUSG00000064090 [Q8BN21-1]
GeneIDi69922
KEGGimmu:69922
UCSCiuc007igf.2 mouse [Q8BN21-1]
uc007igg.2 mouse [Q8BN21-3]
uc011xsk.2 mouse [Q8BN21-2]

Organism-specific databases

CTDi7444
MGIiMGI:1917172 Vrk2

Phylogenomic databases

eggNOGiKOG1164 Eukaryota
ENOG410XPGP LUCA
GeneTreeiENSGT00920000148988
HOVERGENiHBG007532
InParanoidiQ8BN21
KOiK08816
OMAiSSCCEIA
OrthoDBiEOG091G05V5
PhylomeDBiQ8BN21
TreeFamiTF106473

Enzyme and pathway databases

ReactomeiR-MMU-2995383 Initiation of Nuclear Envelope Reformation

Miscellaneous databases

PROiPR:Q8BN21
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000064090 Expressed in 224 organ(s), highest expression level in blood
CleanExiMM_VRK2
ExpressionAtlasiQ8BN21 baseline and differential
GenevisibleiQ8BN21 MM

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiVRK2_MOUSE
AccessioniPrimary (citable) accession number: Q8BN21
Secondary accession number(s): Q3U415
, Q5F1Z5, Q5SP88, Q8BPU8, Q8CJ46, Q91WS1, Q9CZF9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: October 10, 2003
Last modified: September 12, 2018
This is version 143 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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