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Protein

Neuronal acetylcholine receptor subunit beta-3

Gene

Chrnb3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel, Ligand-gated ion channel, Receptor
Biological processIon transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-629594 Highly calcium permeable postsynaptic nicotinic acetylcholine receptors
R-MMU-629597 Highly calcium permeable nicotinic acetylcholine receptors

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal acetylcholine receptor subunit beta-3
Gene namesi
Name:Chrnb3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:106212 Chrnb3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini31 – 238ExtracellularSequence analysisAdd BLAST208
Transmembranei239 – 263HelicalSequence analysisAdd BLAST25
Transmembranei271 – 288HelicalSequence analysisAdd BLAST18
Transmembranei305 – 326HelicalSequence analysisAdd BLAST22
Topological domaini327 – 434CytoplasmicSequence analysisAdd BLAST108
Transmembranei435 – 453HelicalSequence analysisAdd BLAST19

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3885610

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30Sequence analysisAdd BLAST30
ChainiPRO_000000038431 – 464Neuronal acetylcholine receptor subunit beta-3Add BLAST434

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi55N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi159 ↔ 173By similarity
Glycosylationi172N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8BMN3
PRIDEiQ8BMN3

PTM databases

iPTMnetiQ8BMN3
PhosphoSitePlusiQ8BMN3

Expressioni

Gene expression databases

BgeeiENSMUSG00000031492 Expressed in 34 organ(s), highest expression level in Ammon's horn
ExpressionAtlasiQ8BMN3 baseline and differential
GenevisibleiQ8BMN3 MM

Interactioni

Subunit structurei

Neuronal AChR seems to be composed of two different types of subunits: alpha and beta.By similarity

Protein-protein interaction databases

ComplexPortaliCPX-202 Neuronal nicotinic acetylcholine receptor complex, alpha3-alpha6-beta2-beta3
STRINGi10090.ENSMUSP00000052297

Structurei

3D structure databases

ProteinModelPortaliQ8BMN3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3645 Eukaryota
ENOG410XQGR LUCA
GeneTreeiENSGT00760000118930
HOGENOMiHOG000006756
HOVERGENiHBG003756
InParanoidiQ8BMN3
KOiK04814
OMAiLLCMKGH
OrthoDBiEOG091G0R20
PhylomeDBiQ8BMN3
TreeFamiTF315605

Family and domain databases

Gene3Di2.70.170.10, 1 hit
InterProiView protein in InterPro
IPR006202 Neur_chan_lig-bd
IPR036734 Neur_chan_lig-bd_sf
IPR006201 Neur_channel
IPR036719 Neuro-gated_channel_TM_sf
IPR006029 Neurotrans-gated_channel_TM
IPR018000 Neurotransmitter_ion_chnl_CS
IPR002394 Nicotinic_acetylcholine_rcpt
PANTHERiPTHR18945 PTHR18945, 2 hits
PfamiView protein in Pfam
PF02931 Neur_chan_LBD, 1 hit
PF02932 Neur_chan_memb, 1 hit
PRINTSiPR00254 NICOTINICR
PR00252 NRIONCHANNEL
SUPFAMiSSF63712 SSF63712, 1 hit
SSF90112 SSF90112, 1 hit
TIGRFAMsiTIGR00860 LIC, 1 hit
PROSITEiView protein in PROSITE
PS00236 NEUROTR_ION_CHANNEL, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8BMN3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MTGFLRVFLA LSATLSGSWV TLTATAGLSS VAEHEDALLR HLFQGYQKCV
60 70 80 90 100
RPVLNSSDII KVYFGLKISQ LVDVDEKNQL MTTNVWLKQE WTDQKLRWNP
110 120 130 140 150
EDYGGINSIK VPSESLWLPD IVLFENADGR FEGSLMTKAI VKSSGTVSWT
160 170 180 190 200
PPASYKSSCT MDVTFFPFDK QNCSMKFGSW TYDGTMVDLI LINENVDRKD
210 220 230 240 250
FFDNGEWEIL NAKGMKGNRR EGFYSYPFVT YSFVLRRLPL FYTLFLIIPC
260 270 280 290 300
LGLSFLTVLV FYLPSDEGEK LSLSTSVLVS LTVFLLVIEE IIPSSSKVIP
310 320 330 340 350
LIGEYLLFIM IFVTLSIIVT VFVINVHHRS SSTYHPMAPW VKRLFLEKLP
360 370 380 390 400
RWLCMKDPRD RFSFPDGTES KGTVRGKFPG KKKQTPTSDG ERVLVAFLEK
410 420 430 440 450
ASESIRYISR HVKKEHFISQ VVQDWKFVAQ VLDRIFLWLF LTASVLGSVL
460
IFIPALKMWI HRFH
Length:464
Mass (Da):53,112
Last modified:March 1, 2003 - v1
Checksum:i2ECEA3E0DAF2D5EB
GO
Isoform 2 (identifier: Q8BMN3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     75-89: Missing.

Note: No experimental confirmation available.
Show »
Length:449
Mass (Da):51,282
Checksum:iFF54824C5DAAC8E7
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q3UZS0Q3UZS0_MOUSE
Neuronal acetylcholine receptor sub...
Chrnb3
454Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti170K → R in AAL75573 (Ref. 1) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01377575 – 89Missing in isoform 2. 1 PublicationAdd BLAST15

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF467896 mRNA Translation: AAL75573.1
AY574268 mRNA Translation: AAS90364.1
AK017571 mRNA Translation: BAB30812.1
AK030464 mRNA Translation: BAC26973.1
BC058193 mRNA Translation: AAH58193.1
CCDSiCCDS22216.1 [Q8BMN3-2]
CCDS22217.1 [Q8BMN3-1]
RefSeqiNP_081730.1, NM_027454.4 [Q8BMN3-2]
NP_775304.1, NM_173212.4 [Q8BMN3-1]
UniGeneiMm.110444

Genome annotation databases

EnsembliENSMUST00000060943; ENSMUSP00000052297; ENSMUSG00000031492 [Q8BMN3-1]
ENSMUST00000079463; ENSMUSP00000078428; ENSMUSG00000031492 [Q8BMN3-2]
GeneIDi108043
KEGGimmu:108043
UCSCiuc009lit.2 mouse [Q8BMN3-1]
uc009liu.2 mouse [Q8BMN3-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF467896 mRNA Translation: AAL75573.1
AY574268 mRNA Translation: AAS90364.1
AK017571 mRNA Translation: BAB30812.1
AK030464 mRNA Translation: BAC26973.1
BC058193 mRNA Translation: AAH58193.1
CCDSiCCDS22216.1 [Q8BMN3-2]
CCDS22217.1 [Q8BMN3-1]
RefSeqiNP_081730.1, NM_027454.4 [Q8BMN3-2]
NP_775304.1, NM_173212.4 [Q8BMN3-1]
UniGeneiMm.110444

3D structure databases

ProteinModelPortaliQ8BMN3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

ComplexPortaliCPX-202 Neuronal nicotinic acetylcholine receptor complex, alpha3-alpha6-beta2-beta3
STRINGi10090.ENSMUSP00000052297

Chemistry databases

ChEMBLiCHEMBL3885610

PTM databases

iPTMnetiQ8BMN3
PhosphoSitePlusiQ8BMN3

Proteomic databases

PaxDbiQ8BMN3
PRIDEiQ8BMN3

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000060943; ENSMUSP00000052297; ENSMUSG00000031492 [Q8BMN3-1]
ENSMUST00000079463; ENSMUSP00000078428; ENSMUSG00000031492 [Q8BMN3-2]
GeneIDi108043
KEGGimmu:108043
UCSCiuc009lit.2 mouse [Q8BMN3-1]
uc009liu.2 mouse [Q8BMN3-2]

Organism-specific databases

CTDi1142
MGIiMGI:106212 Chrnb3

Phylogenomic databases

eggNOGiKOG3645 Eukaryota
ENOG410XQGR LUCA
GeneTreeiENSGT00760000118930
HOGENOMiHOG000006756
HOVERGENiHBG003756
InParanoidiQ8BMN3
KOiK04814
OMAiLLCMKGH
OrthoDBiEOG091G0R20
PhylomeDBiQ8BMN3
TreeFamiTF315605

Enzyme and pathway databases

ReactomeiR-MMU-629594 Highly calcium permeable postsynaptic nicotinic acetylcholine receptors
R-MMU-629597 Highly calcium permeable nicotinic acetylcholine receptors

Miscellaneous databases

PROiPR:Q8BMN3
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031492 Expressed in 34 organ(s), highest expression level in Ammon's horn
ExpressionAtlasiQ8BMN3 baseline and differential
GenevisibleiQ8BMN3 MM

Family and domain databases

Gene3Di2.70.170.10, 1 hit
InterProiView protein in InterPro
IPR006202 Neur_chan_lig-bd
IPR036734 Neur_chan_lig-bd_sf
IPR006201 Neur_channel
IPR036719 Neuro-gated_channel_TM_sf
IPR006029 Neurotrans-gated_channel_TM
IPR018000 Neurotransmitter_ion_chnl_CS
IPR002394 Nicotinic_acetylcholine_rcpt
PANTHERiPTHR18945 PTHR18945, 2 hits
PfamiView protein in Pfam
PF02931 Neur_chan_LBD, 1 hit
PF02932 Neur_chan_memb, 1 hit
PRINTSiPR00254 NICOTINICR
PR00252 NRIONCHANNEL
SUPFAMiSSF63712 SSF63712, 1 hit
SSF90112 SSF90112, 1 hit
TIGRFAMsiTIGR00860 LIC, 1 hit
PROSITEiView protein in PROSITE
PS00236 NEUROTR_ION_CHANNEL, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiACHB3_MOUSE
AccessioniPrimary (citable) accession number: Q8BMN3
Secondary accession number(s): Q8R5H3, Q9CYK8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 24, 2005
Last sequence update: March 1, 2003
Last modified: November 7, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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