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Protein

[F-actin]-monooxygenase MICAL2

Gene

Mical2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Nuclear monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin to form methionine-sulfoxide, resulting in actin filament disassembly and preventing repolymerization (PubMed:23911929, PubMed:23927065). In the absence of actin, it also functions as a NADPH oxidase producing H2O2 (By similarity). Acts as a key regulator of the SRF signaling pathway elicited by nerve growth factor and serum: mediates oxidation and subsequent depolymerization of nuclear actin, leading to increase MKL1/MRTF-A presence in the nucleus and promote SRF:MKL1/MRTF-A-dependent gene transcription. Does not activate SRF:MKL1/MRTF-A through RhoA (By similarity).By similarity2 Publications

Catalytic activityi

[F-actin]-L-methionine + NADPH + O2 + H+ = [F-actin]-L-methionine-(R)-S-oxide + NADP+ + H2O.2 Publications

Cofactori

FADBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei97FADBy similarity1
Binding sitei183FAD; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei298FADBy similarity1
Binding sitei398FADBy similarity1
Metal bindingi753Zinc 1By similarity1
Metal bindingi756Zinc 1By similarity1
Metal bindingi774Zinc 1; via pros nitrogenBy similarity1
Metal bindingi777Zinc 1By similarity1
Metal bindingi780Zinc 2By similarity1
Metal bindingi783Zinc 2By similarity1
Metal bindingi803Zinc 2By similarity1
Metal bindingi806Zinc 2; via pros nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi116 – 118FADBy similarity3
Nucleotide bindingi123 – 125FADBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActin-binding, Monooxygenase, Oxidoreductase
LigandFAD, Flavoprotein, Metal-binding, NADP, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
[F-actin]-monooxygenase MICAL2 (EC:1.14.13.2252 Publications)
Alternative name(s):
Molecule interacting with CasL protein 2
Short name:
MICAL-2
Short name:
mMical2
Gene namesi
Name:Mical2
Synonyms:Kiaa0750
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2444947 Mical2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000758451 – 960[F-actin]-monooxygenase MICAL2Add BLAST960

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei631PhosphoserineBy similarity1
Isoform 2 (identifier: Q8BML1-2)
Modified residuei812PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8BML1
PeptideAtlasiQ8BML1
PRIDEiQ8BML1

PTM databases

iPTMnetiQ8BML1
PhosphoSitePlusiQ8BML1

Expressioni

Gene expression databases

BgeeiENSMUSG00000038244 Expressed in 158 organ(s), highest expression level in brain
ExpressionAtlasiQ8BML1 baseline and differential
GenevisibleiQ8BML1 MM

Interactioni

Subunit structurei

Interacts with PLXNA4 (PubMed:12110185). Interacts with RAB1B (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000047639

Structurei

3D structure databases

ProteinModelPortaliQ8BML1
SMRiQ8BML1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini516 – 619Calponin-homology (CH)PROSITE-ProRule annotationAdd BLAST104
Domaini751 – 813LIM zinc-bindingPROSITE-ProRule annotationAdd BLAST63

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 494Monooxygenase domainBy similarityAdd BLAST493

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi660 – 681Nuclear localization signalBy similarityAdd BLAST22

Sequence similaritiesi

Belongs to the Mical family.Curated

Keywords - Domaini

LIM domain

Phylogenomic databases

eggNOGiKOG1700 Eukaryota
COG5069 LUCA
GeneTreeiENSGT00760000118856
HOGENOMiHOG000047263
HOVERGENiHBG052474
InParanoidiQ8BML1
KOiK19947
OMAiCSVCATT
OrthoDBiEOG091G009H
TreeFamiTF324129

Family and domain databases

CDDicd00014 CH, 1 hit
Gene3Di1.10.418.10, 1 hit
InterProiView protein in InterPro
IPR001715 CH-domain
IPR036872 CH_dom_sf
IPR002938 FAD-bd
IPR036188 FAD/NAD-bd_sf
IPR029939 MICAL2
IPR001781 Znf_LIM
PANTHERiPTHR44265:SF3 PTHR44265:SF3, 2 hits
PfamiView protein in Pfam
PF00307 CH, 1 hit
PF01494 FAD_binding_3, 1 hit
PF00412 LIM, 1 hit
SMARTiView protein in SMART
SM00033 CH, 1 hit
SM00132 LIM, 1 hit
SUPFAMiSSF47576 SSF47576, 1 hit
SSF51905 SSF51905, 1 hit
PROSITEiView protein in PROSITE
PS50021 CH, 1 hit
PS00478 LIM_DOMAIN_1, 1 hit
PS50023 LIM_DOMAIN_2, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8BML1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGENEDEKQA QASQVFENFV QATTCKGTLQ AFNILTCLLD LDPLDHRNFY
60 70 80 90 100
SQLKSKVNTW KAKALWHKLD KRGSHKEYKR GKACSNTKCL IVGGGPCGLR
110 120 130 140 150
TAIELAYLGA KVVVVEKRDT FSRNNVLHLW PFTIHDLRGL GAKKFYGKFC
160 170 180 190 200
AGSIDHISIR QLQLILFKVA LMLGVEVHVN VEFVRVLEPP EDQENQKVGW
210 220 230 240 250
RAEFLPADHA LSDFEFDVII GADGHRNTLE GFRRKEFRGK LAIAITANFI
260 270 280 290 300
NRNSTAEAKV EEISGVAFIF NQKFFQDLKE ETGIDLENIV YYKDSTHYFV
310 320 330 340 350
MTAKKQSLLD KGVILNDYID TEMLLCSENV NQDNLLSYAR EAADFATNYQ
360 370 380 390 400
LPSLDFAINH NGQPDVAMFD FTSMYASENA ALMRERQAHQ LLVALVGDSL
410 420 430 440 450
LEPFWPMGTG CARGFLAAFD TAWMVKSWDQ GTPPLEVLAE RESLYRLLPQ
460 470 480 490 500
TTPENINKNF EQYTLDPATR YPNLNLHCVR PHQVKHLYIT KEMDRFPLER
510 520 530 540 550
WGSVRRSVSL SRRESDIRPN KLLTWCQQQT KGYQHVRVTD LTTSWRSGLA
560 570 580 590 600
LCAIIHSFRP ELINFDSLNE DDAVENNQLA FDVAKREFGI LPVTTGKEMA
610 620 630 640 650
STQEPDKLSM VMYLSKFYEL FRGTPLRPMD SWRKNYGENA DFGLGKTFIQ
660 670 680 690 700
NNYLNLTLPR KRTPRVDTQT EENDMNKRRR QGFNHLEELP SFSSRSLGSS
710 720 730 740 750
QEYAKESGSQ NKVKHMANQL LAKFEENTRN PSVVKQESPR KAFPLSLGGR
760 770 780 790 800
DTCYFCKKRV YMIERLSAEG HFFHQECFRC SVCSATLRLA AYAFDCDEGK
810 820 830 840 850
FYCKPHFVHC KTSSKQRKRR AELNQQREEE GTWQEQEAPR RDVPTESSCA
860 870 880 890 900
VAAISTPEGS PPGTSTSFFR KALSWPLRLT RGLLNLPQSL LRWMQGLQEA
910 920 930 940 950
AGHHVRDNAH NYCFMFELLS LGLLLLWAFS KVLAAMYRES EESLENIRSW
960
LLRFIPVKLQ
Length:960
Mass (Da):110,072
Last modified:March 1, 2003 - v1
Checksum:i0D70BBA2144730E2
GO
Isoform 2 (identifier: Q8BML1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     736-736: Q → QDCRRVSGIG...PKTQATSPDL
     863-960: GTSTSFFRKA...LLRFIPVKLQ → VRFSLPVLHPLLG

Note: No experimental confirmation available.Combined sources
Show »
Length:1,102
Mass (Da):124,257
Checksum:iF87D341BF3BA9E46
GO
Isoform 3 (identifier: Q8BML1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     799-960: GKFYCKPHFV...LLRFIPVKLQ → GNPRGQESTSLWPWSQM

Note: No experimental confirmation available.
Show »
Length:815
Mass (Da):93,331
Checksum:iAD9FB09B5ED58458
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6WY65F6WY65_MOUSE
[F-actin]-monooxygenase MICAL2
Mical2
137Annotation score:

Sequence cautioni

The sequence BAD90416 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_042597736Q → QDCRRVSGIGKPVLCSASRP PGTSCCPKLEESTPRLPPPL KRQFSSTVATGQVLRELNQV PASGECPSRPWRARAKSDLQ LGGVENLATLPRTCQGALAL SGVLRRLQQVEEKVLQKRAQ NLANREFHTKNIKEKAAHLA SMFGHGDLPQDKLLSKRVPH AHPPSPPSCLPSPHPAAASS PPAADSVSPARKLTVGKVSS GIGAAAEVLVNLYLNDHRPK TQATSPDL in isoform 2. 1 Publication1
Alternative sequenceiVSP_042598799 – 960GKFYC…PVKLQ → GNPRGQESTSLWPWSQM in isoform 3. 1 PublicationAdd BLAST162
Alternative sequenceiVSP_042599863 – 960GTSTS…PVKLQ → VRFSLPVLHPLLG in isoform 2. 1 PublicationAdd BLAST98

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK220353 mRNA Translation: BAD90416.1 Different initiation.
AK030608 mRNA Translation: BAC27044.1
AK138935 mRNA Translation: BAE23824.1
AK143191 mRNA Translation: BAE25298.1
AK163078 mRNA Translation: BAE37182.1
AC100153 Genomic DNA No translation available.
AC132392 Genomic DNA No translation available.
AC166834 Genomic DNA No translation available.
BC111895 mRNA Translation: AAI11896.1
BC112415 mRNA Translation: AAI12416.1
CCDSiCCDS21753.1 [Q8BML1-1]
CCDS57581.1 [Q8BML1-2]
RefSeqiNP_001180234.1, NM_001193305.1 [Q8BML1-2]
NP_796256.1, NM_177282.5 [Q8BML1-1]
XP_006508017.1, XM_006507954.1 [Q8BML1-2]
UniGeneiMm.488195
Mm.52312

Genome annotation databases

EnsembliENSMUST00000037991; ENSMUSP00000047639; ENSMUSG00000038244 [Q8BML1-2]
ENSMUST00000050149; ENSMUSP00000051163; ENSMUSG00000038244 [Q8BML1-1]
GeneIDi320878
KEGGimmu:320878
UCSCiuc009jgm.2 mouse [Q8BML1-3]
uc009jgn.2 mouse [Q8BML1-1]
uc009jgo.2 mouse [Q8BML1-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK220353 mRNA Translation: BAD90416.1 Different initiation.
AK030608 mRNA Translation: BAC27044.1
AK138935 mRNA Translation: BAE23824.1
AK143191 mRNA Translation: BAE25298.1
AK163078 mRNA Translation: BAE37182.1
AC100153 Genomic DNA No translation available.
AC132392 Genomic DNA No translation available.
AC166834 Genomic DNA No translation available.
BC111895 mRNA Translation: AAI11896.1
BC112415 mRNA Translation: AAI12416.1
CCDSiCCDS21753.1 [Q8BML1-1]
CCDS57581.1 [Q8BML1-2]
RefSeqiNP_001180234.1, NM_001193305.1 [Q8BML1-2]
NP_796256.1, NM_177282.5 [Q8BML1-1]
XP_006508017.1, XM_006507954.1 [Q8BML1-2]
UniGeneiMm.488195
Mm.52312

3D structure databases

ProteinModelPortaliQ8BML1
SMRiQ8BML1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000047639

PTM databases

iPTMnetiQ8BML1
PhosphoSitePlusiQ8BML1

Proteomic databases

PaxDbiQ8BML1
PeptideAtlasiQ8BML1
PRIDEiQ8BML1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000037991; ENSMUSP00000047639; ENSMUSG00000038244 [Q8BML1-2]
ENSMUST00000050149; ENSMUSP00000051163; ENSMUSG00000038244 [Q8BML1-1]
GeneIDi320878
KEGGimmu:320878
UCSCiuc009jgm.2 mouse [Q8BML1-3]
uc009jgn.2 mouse [Q8BML1-1]
uc009jgo.2 mouse [Q8BML1-2]

Organism-specific databases

CTDi9645
MGIiMGI:2444947 Mical2
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1700 Eukaryota
COG5069 LUCA
GeneTreeiENSGT00760000118856
HOGENOMiHOG000047263
HOVERGENiHBG052474
InParanoidiQ8BML1
KOiK19947
OMAiCSVCATT
OrthoDBiEOG091G009H
TreeFamiTF324129

Miscellaneous databases

ChiTaRSiMical2 mouse
PROiPR:Q8BML1
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038244 Expressed in 158 organ(s), highest expression level in brain
ExpressionAtlasiQ8BML1 baseline and differential
GenevisibleiQ8BML1 MM

Family and domain databases

CDDicd00014 CH, 1 hit
Gene3Di1.10.418.10, 1 hit
InterProiView protein in InterPro
IPR001715 CH-domain
IPR036872 CH_dom_sf
IPR002938 FAD-bd
IPR036188 FAD/NAD-bd_sf
IPR029939 MICAL2
IPR001781 Znf_LIM
PANTHERiPTHR44265:SF3 PTHR44265:SF3, 2 hits
PfamiView protein in Pfam
PF00307 CH, 1 hit
PF01494 FAD_binding_3, 1 hit
PF00412 LIM, 1 hit
SMARTiView protein in SMART
SM00033 CH, 1 hit
SM00132 LIM, 1 hit
SUPFAMiSSF47576 SSF47576, 1 hit
SSF51905 SSF51905, 1 hit
PROSITEiView protein in PROSITE
PS50021 CH, 1 hit
PS00478 LIM_DOMAIN_1, 1 hit
PS50023 LIM_DOMAIN_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMICA2_MOUSE
AccessioniPrimary (citable) accession number: Q8BML1
Secondary accession number(s): F6ZD54
, F8WJF1, Q14DP1, Q3TR48, Q3UPU6, Q5DU17
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: March 1, 2003
Last modified: November 7, 2018
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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