UniProtKB - Q8BMK4 (CKAP4_MOUSE)
Protein
Cytoskeleton-associated protein 4
Gene
Ckap4
Organism
Mus musculus (Mouse)
Status
Functioni
High-affinity epithelial cell surface receptor for APF.By similarity
Mediates the anchoring of the endoplasmic reticulum to microtubules.By similarity
Enzyme and pathway databases
Reactomei | R-MMU-381426, Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) R-MMU-5683826, Surfactant metabolism R-MMU-6798695, Neutrophil degranulation R-MMU-8957275, Post-translational protein phosphorylation |
Names & Taxonomyi
Protein namesi | Recommended name: Cytoskeleton-associated protein 4Alternative name(s): 63-kDa cytoskeleton-linking membrane protein Short name: Climp-63 Short name: p63 |
Gene namesi | Name:Ckap4 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2444926, Ckap4 |
Subcellular locationi
Plasma membrane
Cytoskeleton
Endoplasmic reticulum
Other locations
Note: Translocates to the perinuclear region upon APF-stimulation.By similarity
Cytoskeleton
- cytoskeleton Source: UniProtKB-SubCell
Cytosol
- cytosol Source: MGI
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
- rough endoplasmic reticulum Source: MGI
Nucleus
- nuclear speck Source: MGI
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- cell surface Source: MGI
- cytoplasmic ribonucleoprotein granule Source: MGI
- integral component of membrane Source: UniProtKB-KW
- lipid droplet Source: MGI
- perinuclear region of cytoplasm Source: UniProtKB-SubCell
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 85 | CytoplasmicSequence analysisAdd BLAST | 85 | |
Transmembranei | 86 – 108 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 109 – 575 | ExtracellularSequence analysisAdd BLAST | 467 |
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Cytoskeleton, Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000252418 | 1 – 575 | Cytoskeleton-associated protein 4Add BLAST | 575 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 3 | PhosphoserineBy similarity | 1 | |
Modified residuei | 17 | PhosphoserineBy similarity | 1 | |
Modified residuei | 19 | PhosphoserineBy similarity | 1 | |
Modified residuei | 21 | N6-acetyllysineCombined sources | 1 | |
Lipidationi | 79 | S-palmitoyl cysteine; by ZDHHC2By similarity | 1 | |
Modified residuei | 211 | PhosphoserineBy similarity | 1 | |
Modified residuei | 292 | PhosphoserineBy similarity | 1 | |
Modified residuei | 367 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Reversibly palmitoylated. Palmitoylation at Cys-79 by DHHC2 is required for its trafficking from the ER to the plasma membrane and for its perinuclear localization (By similarity).By similarity
Increased phosphorylation during mitosis prevents binding to microtubules.By similarity
Keywords - PTMi
Acetylation, Lipoprotein, Palmitate, PhosphoproteinProteomic databases
EPDi | Q8BMK4 |
jPOSTi | Q8BMK4 |
MaxQBi | Q8BMK4 |
PaxDbi | Q8BMK4 |
PeptideAtlasi | Q8BMK4 |
PRIDEi | Q8BMK4 |
PTM databases
iPTMneti | Q8BMK4 |
PhosphoSitePlusi | Q8BMK4 |
SwissPalmi | Q8BMK4 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000046841, Expressed in urinary bladder and 289 other tissues |
Genevisiblei | Q8BMK4, MM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 229721, 6 interactors |
IntActi | Q8BMK4, 6 interactors |
MINTi | Q8BMK4 |
STRINGi | 10090.ENSMUSP00000050336 |
Miscellaneous databases
RNActi | Q8BMK4, protein |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 125 – 193 | Sequence analysisAdd BLAST | 69 | |
Coiled coili | 236 – 438 | Sequence analysisAdd BLAST | 203 | |
Coiled coili | 507 – 575 | Sequence analysisAdd BLAST | 69 |
Keywords - Domaini
Coiled coil, Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QVCP, Eukaryota |
GeneTreei | ENSGT00390000015968 |
HOGENOMi | CLU_032481_0_0_1 |
InParanoidi | Q8BMK4 |
OMAi | SEGYKQD |
OrthoDBi | 1255457at2759 |
PhylomeDBi | Q8BMK4 |
TreeFami | TF332395 |
Family and domain databases
InterProi | View protein in InterPro IPR028428, Ckap4 |
PANTHERi | PTHR45161, PTHR45161, 1 hit |
i Sequence
Sequence statusi: Complete.
Q8BMK4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPSAKQRGSK GGHGAASPSD KGAHPSGGAD DVAKKPPAAP QQPQPPAPHP
60 70 80 90 100
PQHPQNQAHR GGHRGRSSAA TANASSASCS RRLGRVLNFL FYLSLVAAAA
110 120 130 140 150
FSGWYVHHVL EEVQQVRRGH QDFSRQRDEL GQGLQGVEQK VQSLQATFGT
160 170 180 190 200
FESLLRNSQH KQDLTEKAVK EGESELNRIS EVLQKLQNEI LKDLSDGIHV
210 220 230 240 250
VKDARERDFT SLENTVEERL TELTKSINDN IAIFTDVQKR SQKEINEVKM
260 270 280 290 300
KVASLEESKG DRSQDVKTLK DAVKEVQASM MSRERDIEAL KSSLQTMESD
310 320 330 340 350
VYTEVRELVS LKQEQQAFKQ AADSERLALQ ALTEKLLRSE ESSSRLPEDI
360 370 380 390 400
RRLEEELQQL KVGAHGSEEG AVFKDSKALE ELQRQIEGLG ARLQYVEDGV
410 420 430 440 450
YSMQVASARH TESLESLLSK SQEYEQRLAM LQEHVGNLGS SSDLASTVRS
460 470 480 490 500
LGETQLALSS DLKELKQSLG ELPGTVESLQ EQVLSLLSQD QAQAEGLPPQ
510 520 530 540 550
DFLDRLSSLD NLKSSVSQVE SDLKMLRTAV DSLVAYSVKI ETNENNLESA
560 570
KGLLDDLRND LDRLFLKVEK IHEKI
Sequence cautioni
The sequence AAH25522 differs from that shown. Contaminating sequence. Vector contamination at the N-terminus.Curated
The sequence AAH25522 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence BAC41005 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 243 | K → M in AAH25522 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 281 | M → I in BAC41005 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC140333 Genomic DNA No translation available. AC150314 Genomic DNA No translation available. BC025522 mRNA Translation: AAH25522.1 Different initiation. BC138318 mRNA Translation: AAI38319.1 BC138320 mRNA Translation: AAI38321.1 AK030708 mRNA Translation: BAC27092.1 AK089935 mRNA Translation: BAC41005.1 Different initiation. |
CCDSi | CCDS24080.1 |
RefSeqi | NP_780660.1, NM_175451.1 |
Genome annotation databases
Ensembli | ENSMUST00000053871; ENSMUSP00000050336; ENSMUSG00000046841 ENSMUST00000167671; ENSMUSP00000130304; ENSMUSG00000046841 |
GeneIDi | 216197 |
KEGGi | mmu:216197 |
UCSCi | uc007gkq.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC140333 Genomic DNA No translation available. AC150314 Genomic DNA No translation available. BC025522 mRNA Translation: AAH25522.1 Different initiation. BC138318 mRNA Translation: AAI38319.1 BC138320 mRNA Translation: AAI38321.1 AK030708 mRNA Translation: BAC27092.1 AK089935 mRNA Translation: BAC41005.1 Different initiation. |
CCDSi | CCDS24080.1 |
RefSeqi | NP_780660.1, NM_175451.1 |
3D structure databases
SMRi | Q8BMK4 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 229721, 6 interactors |
IntActi | Q8BMK4, 6 interactors |
MINTi | Q8BMK4 |
STRINGi | 10090.ENSMUSP00000050336 |
PTM databases
iPTMneti | Q8BMK4 |
PhosphoSitePlusi | Q8BMK4 |
SwissPalmi | Q8BMK4 |
Proteomic databases
EPDi | Q8BMK4 |
jPOSTi | Q8BMK4 |
MaxQBi | Q8BMK4 |
PaxDbi | Q8BMK4 |
PeptideAtlasi | Q8BMK4 |
PRIDEi | Q8BMK4 |
Protocols and materials databases
Antibodypediai | 613, 274 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000053871; ENSMUSP00000050336; ENSMUSG00000046841 ENSMUST00000167671; ENSMUSP00000130304; ENSMUSG00000046841 |
GeneIDi | 216197 |
KEGGi | mmu:216197 |
UCSCi | uc007gkq.1, mouse |
Organism-specific databases
CTDi | 10970 |
MGIi | MGI:2444926, Ckap4 |
Phylogenomic databases
eggNOGi | ENOG502QVCP, Eukaryota |
GeneTreei | ENSGT00390000015968 |
HOGENOMi | CLU_032481_0_0_1 |
InParanoidi | Q8BMK4 |
OMAi | SEGYKQD |
OrthoDBi | 1255457at2759 |
PhylomeDBi | Q8BMK4 |
TreeFami | TF332395 |
Enzyme and pathway databases
Reactomei | R-MMU-381426, Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) R-MMU-5683826, Surfactant metabolism R-MMU-6798695, Neutrophil degranulation R-MMU-8957275, Post-translational protein phosphorylation |
Miscellaneous databases
BioGRID-ORCSi | 216197, 0 hits in 17 CRISPR screens |
ChiTaRSi | Ckap4, mouse |
PROi | PR:Q8BMK4 |
RNActi | Q8BMK4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000046841, Expressed in urinary bladder and 289 other tissues |
Genevisiblei | Q8BMK4, MM |
Family and domain databases
InterProi | View protein in InterPro IPR028428, Ckap4 |
PANTHERi | PTHR45161, PTHR45161, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CKAP4_MOUSE | |
Accessioni | Q8BMK4Primary (citable) accession number: Q8BMK4 Secondary accession number(s): B2RRB4, Q8BTK8, Q8R3F2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 17, 2006 |
Last sequence update: | October 17, 2006 | |
Last modified: | December 2, 2020 | |
This is version 131 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot