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UniProtKB - Q8BMJ8 (SP8_MOUSE)
Protein
Transcription factor Sp8
Gene
Sp8
Organism
Mus musculus (Mouse)
Status
Functioni
Transcription factor which plays a key role in limb development. Positively regulates FGF8 expression in the apical ectodermal ridge (AER) and contributes to limb outgrowth in embryos.
1 PublicationRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 356 – 380 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 386 – 410 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 416 – 438 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 |
GO - Molecular functioni
- DNA binding Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- sequence-specific double-stranded DNA binding Source: MGI
GO - Biological processi
- dorsal/ventral pattern formation Source: MGI
- embryonic limb morphogenesis Source: UniProtKB
- proximal/distal pattern formation Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | DNA-binding |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription factor Sp8 |
Gene namesi | Name:Sp8 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2443471, Sp8 |
VEuPathDBi | HostDB:ENSMUSG00000048562 |
Subcellular locationi
Nucleus
- Nucleus Curated
Nucleus
- nucleus Source: UniProtKB-SubCell
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000047153 | 1 – 486 | Transcription factor Sp8Add BLAST | 486 |
Proteomic databases
MaxQBi | Q8BMJ8 |
PaxDbi | Q8BMJ8 |
PRIDEi | Q8BMJ8 |
ProteomicsDBi | 257332 |
PTM databases
iPTMneti | Q8BMJ8 |
PhosphoSitePlusi | Q8BMJ8 |
Expressioni
Developmental stagei
Expression is detected in the forebrain, midbrain/hindbrain boundary and neural tube. At the stage of limb bud outgrowth, expressed in a scattered manner in the ventral ectoderm, and is later confined to the apical ectodermal ridge (AER).1 Publication
Gene expression databases
Bgeei | ENSMUSG00000048562, Expressed in rostral migratory stream and 100 other tissues |
ExpressionAtlasi | Q8BMJ8, baseline and differential |
Genevisiblei | Q8BMJ8, MM |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000065746 |
Miscellaneous databases
RNActi | Q8BMJ8, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 46 | DisorderedSequence analysisAdd BLAST | 46 | |
Regioni | 136 – 157 | DisorderedSequence analysisAdd BLAST | 22 | |
Regioni | 294 – 324 | DisorderedSequence analysisAdd BLAST | 31 | |
Regioni | 432 – 486 | DisorderedSequence analysisAdd BLAST | 55 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 208 – 216 | 9aaTADBy similarity | 9 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 8 – 46 | Polar residuesSequence analysisAdd BLAST | 39 |
Domaini
The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.By similarity
Sequence similaritiesi
Belongs to the Sp1 C2H2-type zinc-finger protein family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 356 – 380 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 386 – 410 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 416 – 438 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000162033 |
HOGENOMi | CLU_019484_4_1_1 |
InParanoidi | Q8BMJ8 |
OMAi | AGMHAQM |
OrthoDBi | 664813at2759 |
PhylomeDBi | Q8BMJ8 |
TreeFami | TF350150 |
Family and domain databases
InterProi | View protein in InterPro IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00096, zf-C2H2, 2 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 3 hits |
SUPFAMi | SSF57667, SSF57667, 2 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 3 hits PS50157, ZINC_FINGER_C2H2_2, 3 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q8BMJ8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLAATCNKIG SPSPSPSSLS DSSSSFGKGF HPWKRSSSSS SGSCNVVGSS
60 70 80 90 100
LSSFGVSGAS RNGGSSSAAA AAAAAAAAAA ALVSDSFSCG GSPGSSAFSL
110 120 130 140 150
TSSSAAAAAA AAAAAASSSP FANDYSVFQA PGVSGGSGGG GGGGGGGSGA
160 170 180 190 200
HSQDSSHQPV FISKVHTSVD GLQGIYPRVG MAHPYESWFK PSHPGLGAAA
210 220 230 240 250
DVGSAGASSW WDVGAGWIDV QNPNGAAALP GSLHPAAGGL QTSLHSPLGG
260 270 280 290 300
YNSDYSGLSH SAFSSGASSH LLSPAGQHLM DGFKPVLPGS YPDSAPSPLA
310 320 330 340 350
GAGSSMLSAG PAAQLGGSPR SSARRYSGRA TCDCPNCQEA ERLGPAGASL
360 370 380 390 400
RRKGLHSCHI PGCGKVYGKT SHLKAHLRWH TGERPFVCNW LFCGKRFTRS
410 420 430 440 450
DELQRHLRTH TGEKRFACPV CNKRFMRSDH LSKHVKTHSG GGGSAGSGGG
460 470 480
KKGSDTDSEH SAAGSPPCHS PELLQPPEPG HRNGLE
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1Y7VJL2 | A0A1Y7VJL2_MOUSE | Transcription factor Sp8 | Sp8 | 181 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK030745 mRNA Translation: BAC27113.1 AK053093 mRNA Translation: BAC35264.1 |
CCDSi | CCDS26213.1 |
RefSeqi | NP_796056.2, NM_177082.4 XP_006516046.1, XM_006515983.3 XP_006516047.1, XM_006515984.3 XP_006516048.1, XM_006515985.3 XP_006516049.1, XM_006515986.3 XP_006516050.1, XM_006515987.3 XP_006516051.1, XM_006515988.3 XP_011242426.1, XM_011244124.2 |
Genome annotation databases
Ensembli | ENSMUST00000063918; ENSMUSP00000065746; ENSMUSG00000048562 |
GeneIDi | 320145 |
KEGGi | mmu:320145 |
UCSCi | uc007pig.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK030745 mRNA Translation: BAC27113.1 AK053093 mRNA Translation: BAC35264.1 |
CCDSi | CCDS26213.1 |
RefSeqi | NP_796056.2, NM_177082.4 XP_006516046.1, XM_006515983.3 XP_006516047.1, XM_006515984.3 XP_006516048.1, XM_006515985.3 XP_006516049.1, XM_006515986.3 XP_006516050.1, XM_006515987.3 XP_006516051.1, XM_006515988.3 XP_011242426.1, XM_011244124.2 |
3D structure databases
SMRi | Q8BMJ8 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000065746 |
PTM databases
iPTMneti | Q8BMJ8 |
PhosphoSitePlusi | Q8BMJ8 |
Proteomic databases
MaxQBi | Q8BMJ8 |
PaxDbi | Q8BMJ8 |
PRIDEi | Q8BMJ8 |
ProteomicsDBi | 257332 |
Protocols and materials databases
Antibodypediai | 53277, 68 antibodies from 15 providers |
DNASUi | 320145 |
Genome annotation databases
Ensembli | ENSMUST00000063918; ENSMUSP00000065746; ENSMUSG00000048562 |
GeneIDi | 320145 |
KEGGi | mmu:320145 |
UCSCi | uc007pig.2, mouse |
Organism-specific databases
CTDi | 221833 |
MGIi | MGI:2443471, Sp8 |
VEuPathDBi | HostDB:ENSMUSG00000048562 |
Phylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000162033 |
HOGENOMi | CLU_019484_4_1_1 |
InParanoidi | Q8BMJ8 |
OMAi | AGMHAQM |
OrthoDBi | 664813at2759 |
PhylomeDBi | Q8BMJ8 |
TreeFami | TF350150 |
Miscellaneous databases
BioGRID-ORCSi | 320145, 3 hits in 63 CRISPR screens |
PROi | PR:Q8BMJ8 |
RNActi | Q8BMJ8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000048562, Expressed in rostral migratory stream and 100 other tissues |
ExpressionAtlasi | Q8BMJ8, baseline and differential |
Genevisiblei | Q8BMJ8, MM |
Family and domain databases
InterProi | View protein in InterPro IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00096, zf-C2H2, 2 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 3 hits |
SUPFAMi | SSF57667, SSF57667, 2 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 3 hits PS50157, ZINC_FINGER_C2H2_2, 3 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | SP8_MOUSE | |
Accessioni | Q8BMJ8Primary (citable) accession number: Q8BMJ8 Secondary accession number(s): Q8BWA3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 16, 2003 |
Last sequence update: | March 1, 2003 | |
Last modified: | February 23, 2022 | |
This is version 135 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families