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Protein

Eukaryotic translation initiation factor 4E type 2

Gene

Eif4e2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation (PubMed:15153109). Acts as a repressor of translation initiation (By similarity). In contrast to EIF4E, it is unable to bind eIF4G (EIF4G1, EIF4G2 or EIF4G3), suggesting that it acts by competing with EIF4E and block assembly of eIF4F at the cap (PubMed:15153109).By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei1107-methylguanosine-containing mRNA capBy similarity1

GO - Molecular functioni

GO - Biological processi

  • in utero embryonic development Source: MGI
  • negative regulation of translation Source: MGI

Keywordsi

Molecular functionInitiation factor, RNA-binding
Biological processProtein biosynthesis, Translation regulation

Enzyme and pathway databases

ReactomeiR-MMU-1169408 ISG15 antiviral mechanism

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 4E type 2
Short name:
eIF-4E type 2
Short name:
eIF4E type 2
Short name:
eIF4E-2
Short name:
mRNA cap-binding protein type 2
Alternative name(s):
Eukaryotic translation initiation factor 4E-like 3
eIF4E-like protein 4E-LP
Gene namesi
Name:Eif4e2
Synonyms:Eif4el3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1914440 Eif4e2

Subcellular locationi

Pathology & Biotechi

Disruption phenotypei

Lethality; mice die just before birth.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001936651 – 245Eukaryotic translation initiation factor 4E type 2Add BLAST245

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei13PhosphoserineBy similarity1
Modified residuei134N6-acetyllysine; alternateBy similarity1
Cross-linki134Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ISG15); alternateBy similarity
Cross-linki222Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ISG15)By similarity

Post-translational modificationi

Ubiquitinated by ARIH1. The consequences of ubiquitination are however unclear: according to a report, EIF4E2 ubiquitination leads to promote EIF4E2 cap-binding and protein translation arrest. According to another report ubiquitination leads to its subsequent degradation.By similarity
ISGylation enhances its cap structure-binding activity and translation-inhibition activity.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8BMB3
MaxQBiQ8BMB3
PaxDbiQ8BMB3
PeptideAtlasiQ8BMB3
PRIDEiQ8BMB3

PTM databases

iPTMnetiQ8BMB3
PhosphoSitePlusiQ8BMB3

Expressioni

Tissue specificityi

Widely expressed with highest levels in testis, kidney and liver.1 Publication

Gene expression databases

BgeeiENSMUSG00000026254 Expressed in 287 organ(s), highest expression level in skin of back
ExpressionAtlasiQ8BMB3 baseline and differential
GenevisibleiQ8BMB3 MM

Interactioni

Subunit structurei

Interacts with EIF4EBP1, EIF4EBP2 and EIF4EBP3 (PubMed:15153109). Does not interact with eIF4G (EIF4G1, EIF4G2 or EIF4G3) (PubMed:15153109). Component of the 4EHP-GYF2 complex, at least composed of EIF4E2, GIGYF2 and ZNF598 (By similarity). Interacts with GIGYF2 (via the 4EHP-binding motif); the interaction is direct (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ8BMB3, 3 interactors
MINTiQ8BMB3
STRINGi10090.ENSMUSP00000108859

Structurei

3D structure databases

ProteinModelPortaliQ8BMB3
SMRiQ8BMB3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni54 – 57EIF4EBP1/2/3 bindingBy similarity4
Regioni78 – 797-methylguanosine-containing mRNA cap bindingBy similarity2
Regioni95 – 99EIF4EBP1/2/3 bindingBy similarity5
Regioni124 – 1257-methylguanosine-containing mRNA cap bindingBy similarity2
Regioni150 – 157EIF4EBP1/2/3 bindingBy similarity8
Regioni174 – 1797-methylguanosine-containing mRNA cap bindingBy similarity6
Regioni222 – 2247-methylguanosine-containing mRNA cap bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1669 Eukaryota
COG5053 LUCA
GeneTreeiENSGT00520000055549
HOGENOMiHOG000186751
HOVERGENiHBG107087
InParanoidiQ8BMB3
KOiK03259
TreeFamiTF101529

Family and domain databases

Gene3Di3.30.760.10, 1 hit
InterProiView protein in InterPro
IPR023398 TIF_eIF4e-like
IPR001040 TIF_eIF_4E
IPR019770 TIF_eIF_4E_CS
PANTHERiPTHR11960 PTHR11960, 1 hit
PfamiView protein in Pfam
PF01652 IF4E, 1 hit
SUPFAMiSSF55418 SSF55418, 1 hit
PROSITEiView protein in PROSITE
PS00813 IF4E, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 7 potential isoforms that are computationally mapped.Show allAlign All

Q8BMB3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNNKFDALKD DDSGDHDQNE ENSTQKDGEK EKTDRDKSQS SGKRKAVVPG
60 70 80 90 100
PAEHPLQYNY TFWYSRRTPG RPTSSQSYEQ NIKQIGTFAS VEQFWKFYSH
110 120 130 140 150
MVRPGDLTGH SDFHLFKEGI KPMWEDDANK NGGKWIIRLR KGLASRCWEN
160 170 180 190 200
LILAMLGEQF MVGEEICGAV VSVRFQEDII SIWNKTASDQ ATTARIRDTL
210 220 230 240
RRVLNLPPNT IMEYKTHTDS IKMPGRLGPQ RLLFQNLWKP RLNVP
Length:245
Mass (Da):28,263
Last modified:March 1, 2003 - v1
Checksum:iAA722843721A01CC
GO

Computationally mapped potential isoform sequencesi

There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3YUV9D3YUV9_MOUSE
Eukaryotic translation initiation f...
Eif4e2
259Annotation score:
D3Z729D3Z729_MOUSE
Eukaryotic translation initiation f...
Eif4e2
236Annotation score:
D3Z730D3Z730_MOUSE
Eukaryotic translation initiation f...
Eif4e2
228Annotation score:
Q0P688Q0P688_MOUSE
Eukaryotic translation initiation f...
Eif4e2
240Annotation score:
G3X9H1G3X9H1_MOUSE
Eukaryotic translation initiation f...
Eif4e2 mCG_16205
234Annotation score:
Q3UUT0Q3UUT0_MOUSE
Eukaryotic translation initiation f...
Eif4e2
233Annotation score:
M0QWD1M0QWD1_MOUSE
Eukaryotic translation initiation f...
Eif4e2
128Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti200L → F in AAC19373 (PubMed:15153109).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF068116 mRNA Translation: AAC19373.1
AK032965 mRNA Translation: BAC28102.1
AK150495 mRNA Translation: BAE29610.1
BC045153 mRNA Translation: AAH45153.1
CCDSiCCDS35652.1
RefSeqiNP_001034259.1, NM_001039170.1
NP_075803.2, NM_023314.3
UniGeneiMm.227183

Genome annotation databases

EnsembliENSMUST00000113233; ENSMUSP00000108859; ENSMUSG00000026254
GeneIDi26987
KEGGimmu:26987
UCSCiuc007bwk.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF068116 mRNA Translation: AAC19373.1
AK032965 mRNA Translation: BAC28102.1
AK150495 mRNA Translation: BAE29610.1
BC045153 mRNA Translation: AAH45153.1
CCDSiCCDS35652.1
RefSeqiNP_001034259.1, NM_001039170.1
NP_075803.2, NM_023314.3
UniGeneiMm.227183

3D structure databases

ProteinModelPortaliQ8BMB3
SMRiQ8BMB3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BMB3, 3 interactors
MINTiQ8BMB3
STRINGi10090.ENSMUSP00000108859

PTM databases

iPTMnetiQ8BMB3
PhosphoSitePlusiQ8BMB3

Proteomic databases

EPDiQ8BMB3
MaxQBiQ8BMB3
PaxDbiQ8BMB3
PeptideAtlasiQ8BMB3
PRIDEiQ8BMB3

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000113233; ENSMUSP00000108859; ENSMUSG00000026254
GeneIDi26987
KEGGimmu:26987
UCSCiuc007bwk.1 mouse

Organism-specific databases

CTDi9470
MGIiMGI:1914440 Eif4e2

Phylogenomic databases

eggNOGiKOG1669 Eukaryota
COG5053 LUCA
GeneTreeiENSGT00520000055549
HOGENOMiHOG000186751
HOVERGENiHBG107087
InParanoidiQ8BMB3
KOiK03259
TreeFamiTF101529

Enzyme and pathway databases

ReactomeiR-MMU-1169408 ISG15 antiviral mechanism

Miscellaneous databases

ChiTaRSiEif4e2 mouse
PROiPR:Q8BMB3
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026254 Expressed in 287 organ(s), highest expression level in skin of back
ExpressionAtlasiQ8BMB3 baseline and differential
GenevisibleiQ8BMB3 MM

Family and domain databases

Gene3Di3.30.760.10, 1 hit
InterProiView protein in InterPro
IPR023398 TIF_eIF4e-like
IPR001040 TIF_eIF_4E
IPR019770 TIF_eIF_4E_CS
PANTHERiPTHR11960 PTHR11960, 1 hit
PfamiView protein in Pfam
PF01652 IF4E, 1 hit
SUPFAMiSSF55418 SSF55418, 1 hit
PROSITEiView protein in PROSITE
PS00813 IF4E, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiIF4E2_MOUSE
AccessioniPrimary (citable) accession number: Q8BMB3
Secondary accession number(s): O88503, Q3UCK2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 11, 2003
Last sequence update: March 1, 2003
Last modified: September 12, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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