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Protein

AT-rich interactive domain-containing protein 5B

Gene

Arid5b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription coactivator that binds to the 5'-AATA[CT]-3' core sequence and plays a key role in adipogenesis and liver development. Acts by forming a complex with phosphorylated PHF2, which mediates demethylation at Lys-337, leading to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver (By similarity). Required for adipogenesis: regulates triglyceride metabolism in adipocytes by regulating expression of adipogenic genes. Overexpression leads to induction of smooth muscle marker genes, suggesting that it may also act as a regulator of smooth muscle cell differentiation and proliferation.By similarity5 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-3214842 HDMs demethylate histones

Names & Taxonomyi

Protein namesi
Recommended name:
AT-rich interactive domain-containing protein 5B
Short name:
ARID domain-containing protein 5B
Alternative name(s):
Developmentally and sexually retarded with transient immune abnormalities protein
Short name:
Desrt
MRF1-like
Modulator recognition factor protein 2
Short name:
MRF-2
Gene namesi
Name:Arid5b
Synonyms:Desrt, Mrf2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:2175912 Arid5b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

High rate of neonatal mortality. Embryonic growth or birth weight are not effected, while lipid accumulation is severely reduced in brown adipose neonates at 24 hours of age. Mice weigh significantly less than controls from postnatal day 5 onward. Adult mice are lean, with significant reductions in brown and white adipose tissues, and in the percentage of body fat. Mice are also resistant to weight gains and obesity when maintained on high-fat diets.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002005821 – 1188AT-rich interactive domain-containing protein 5BAdd BLAST1188

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki130Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei264PhosphoserineBy similarity1
Modified residuei337N6,N6-dimethyllysineBy similarity1
Cross-linki446Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki494Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki496Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki767Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki774Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki803Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki810Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki893Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki916Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki920Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki935Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki988Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki1000Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki1013Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei1032PhosphoserineBy similarity1
Cross-linki1055Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki1070Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei1133PhosphoserineBy similarity1

Post-translational modificationi

Methylation at Lys-337 prevents DNA-binding. Demethylation by PHF2 promotes recruitment of the PHF2-ARID5B complex to promoters (By similarity).By similarity

Keywords - PTMi

Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ8BM75
PeptideAtlasiQ8BM75
PRIDEiQ8BM75

PTM databases

iPTMnetiQ8BM75
PhosphoSitePlusiQ8BM75

Expressioni

Tissue specificityi

Widely expressed. Expressed in lung, heart, small intestine, kidney, muscle and brain. Also expressed in spleen, thymus, endocrine organs and in uterus and testis.2 Publications

Developmental stagei

First detected in the intermediate plate mesoderm, and subsequently in the nephrogenic cords of the urogenital ridges. Expressed in the developing limb. Also expressed in the myotome of the somites from 9.5 dpc, the oro-nasopharyngeal ectoderm and underlying mesenchyme, otic vesicles, the gut and its derivatives, and transiently in the liver at 11.5 dpc.1 Publication

Inductioni

During smooth muscle cell differentiation in vitro. Upon adipogenesis.2 Publications

Gene expression databases

BgeeiENSMUSG00000019947 Expressed in 253 organ(s), highest expression level in head
CleanExiMM_ARID5B
ExpressionAtlasiQ8BM75 baseline and differential
GenevisibleiQ8BM75 MM

Interactioni

Protein-protein interaction databases

BioGridi214670, 1 interactor
IntActiQ8BM75, 1 interactor
MINTiQ8BM75

Structurei

3D structure databases

ProteinModelPortaliQ8BM75
SMRiQ8BM75
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini319 – 411ARIDPROSITE-ProRule annotationAdd BLAST93

Domaini

The ARID domain mediates the interaction with DNA.

Sequence similaritiesi

Belongs to the ARID5B family.Curated

Phylogenomic databases

eggNOGiENOG410IQ7B Eukaryota
ENOG4110NWK LUCA
GeneTreeiENSGT00900000140901
HOVERGENiHBG050575
InParanoidiQ8BM75
OMAiMTMSAPK
OrthoDBiEOG091G0E6N
TreeFamiTF324725

Family and domain databases

Gene3Di1.10.150.60, 1 hit
InterProiView protein in InterPro
IPR030408 ARID5B
IPR001606 ARID_dom
IPR036431 ARID_dom_sf
PANTHERiPTHR13964:SF37 PTHR13964:SF37, 1 hit
PfamiView protein in Pfam
PF01388 ARID, 1 hit
SMARTiView protein in SMART
SM00501 BRIGHT, 1 hit
SUPFAMiSSF46774 SSF46774, 1 hit
PROSITEiView protein in PROSITE
PS51011 ARID, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8BM75-1) [UniParc]FASTAAdd to basket
Also known as: Mrf2alpha, Alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEPNSLQWVG SPCGLHGPYI FYKAFQFHLE GKPRILSLGD FFFVRCTPKD
60 70 80 90 100
PICIAELQLL WEERTSRQLL SSSKLYFLPE DTPQGRNSDH GEDEVIAVSE
110 120 130 140 150
KVIVKLEDLV KWAHSDFSKW RCGLRATPVK TEAFGRNGQK EALLRYRQST
160 170 180 190 200
LNSGLNFKDV LKEKADLGED EEETNVIVLS YPQYCRYRSM LKRIQDKPSS
210 220 230 240 250
ILTDQFALAL GGIAVVSRNP QILYCRDTFD HPTLIENESV CDEFAPNLKG
260 270 280 290 300
RPRKKKTCPQ RRDSFSGSKD PNNNCDGKVI SKVKGEARSA LTKPKNNHNN
310 320 330 340 350
CKKTSNEEKP KLSIGEECRA DEQAFLVALY KYMKERKTPI ERIPYLGFKQ
360 370 380 390 400
INLWTMFQAA QKLGGYETIT ARRQWKHIYD ELGGNPGSTS AATCTRRHYE
410 420 430 440 450
RLILPYERFI KGEEDKPLPP IKPRKQENNT QENENKTKVS GNKRIKQEMA
460 470 480 490 500
KNKKEKENTP KPQDTSEVSS EQRKEEETLN HKSAPEPLPA PEVKGKPEGH
510 520 530 540 550
KDLGARAPVS RADPEKANET DQGSNSEKEA EEMGDKGLAP LLPSPPLPPE
560 570 580 590 600
KDSAPTPGAG KQPLASPSTQ MDSKQEAKPC CFTESPEKDL QGAPFSSFSA
610 620 630 640 650
TKPPLTSQNE AEEEQLPATA NYIANCTVKV DQLGSDDIHT ALKQTPKVLV
660 670 680 690 700
VQSFDMFKDK DLTGPMNENH GLNYTPLLYS RGNPGIMSPL AKKKLLSQVS
710 720 730 740 750
GASLSSSYPY GSPPPLISKK KLIAREDLCS GLSQGHHSQS SDHTAVSRPS
760 770 780 790 800
VIQHVQSFKN KASEDRKSIN DIFKHDKLSR SDAHRCGFSK HQLGSLADSY
810 820 830 840 850
ILKQETQEGK DKLLEKRAVS HAHVPSFLAD FYSSPHLHSL YRHTEHHLHN
860 870 880 890 900
EQSSKYAARD AYQESENGAF LSHKHPEKIH VNYLASLHLQ DKKVAAAEAS
910 920 930 940 950
TDDQPTDLSL PKNPHKLTSK VLGLAHSTSG SQEIKGASQF QVVSNQSRDC
960 970 980 990 1000
HPKACRVSPM TMSGPKKYPE SLARSGKPHQ VRLENFRKME GMVHPILHRK
1010 1020 1030 1040 1050
MSPQNIGAAR PIKRSLEDLD LVIAGKKARA VSPLDPAKEA SGKEKASEQE
1060 1070 1080 1090 1100
SEGNKGAYGG HSGAASEGHK LPLSTPIFPG LYSGSLCNSG LNSRLPAGYS
1110 1120 1130 1140 1150
HSLQYLKNQT VLSPLMQPLA FHSLVMQRGI FTSPTNSQQL YRHLAAATPV
1160 1170 1180
GSSYGDLLHN SIYPLAGINP QAAFPSSQLS SVHPSTKL
Length:1,188
Mass (Da):131,837
Last modified:June 28, 2011 - v3
Checksum:iF0B1A99D3E88D71F
GO
Isoform 2 (identifier: Q8BM75-2) [UniParc]FASTAAdd to basket
Also known as: Mrf2beta, Beta

The sequence of this isoform differs from the canonical sequence as follows:
     1-243: Missing.
     244-244: F → M

Show »
Length:945
Mass (Da):104,015
Checksum:iE43DE82C50BB7C30
GO
Isoform 3 (identifier: Q8BM75-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     245-276: APNLKGRPRKKKTCPQRRDSFSGSKDPNNNCD → GECFSFTLFETRPNESLFSIHGVSSAGEQGLV
     277-1188: Missing.

Note: No experimental confirmation available.
Show »
Length:276
Mass (Da):31,400
Checksum:i04D25CE35FE728DC
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1X7SB62A0A1X7SB62_MOUSE
AT-rich interactive domain-containi...
Arid5b
254Annotation score:

Sequence cautioni

The sequence AAF89682 differs from that shown. Reason: Frameshift at several positions.Curated
The sequence AAF89682 differs from that shown. Reason: Erroneous termination at position 805. Translated as Glu.Curated
The sequence BAC28778 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti111K → T in AAF89682 (PubMed:19468303).Curated1
Sequence conflicti287A → G in AAF89682 (PubMed:19468303).Curated1
Sequence conflicti474K → E in AAM93282 (PubMed:12215486).Curated1
Sequence conflicti474K → E in AAM93269 (PubMed:12215486).Curated1
Sequence conflicti474K → E in AAF89682 (PubMed:19468303).Curated1
Sequence conflicti480N → S in AAM93282 (PubMed:12215486).Curated1
Sequence conflicti480N → S in AAM93269 (PubMed:12215486).Curated1
Sequence conflicti480N → S in AAF89682 (PubMed:19468303).Curated1
Sequence conflicti928T → A in AAM93282 (PubMed:12215486).Curated1
Sequence conflicti928T → A in AAM93269 (PubMed:12215486).Curated1
Sequence conflicti1056G → S in AAM93282 (PubMed:12215486).Curated1
Sequence conflicti1056G → S in AAM93269 (PubMed:12215486).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0093571 – 243Missing in isoform 2. 1 PublicationAdd BLAST243
Alternative sequenceiVSP_009358244F → M in isoform 2. 1 Publication1
Alternative sequenceiVSP_009359245 – 276APNLK…NNNCD → GECFSFTLFETRPNESLFSI HGVSSAGEQGLV in isoform 3. 1 PublicationAdd BLAST32
Alternative sequenceiVSP_009360277 – 1188Missing in isoform 3. 1 PublicationAdd BLAST912

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF280065 mRNA Translation: AAM93269.1
AF401550 mRNA Translation: AAM93282.1
AK017396 mRNA Translation: BAB30727.1
AK031596 mRNA Translation: BAC27467.1
AK034633 mRNA Translation: BAC28778.1 Different initiation.
AC122293 Genomic DNA No translation available.
AC132588 Genomic DNA No translation available.
AF169968 mRNA Translation: AAF89682.1 Sequence problems.
CCDSiCCDS35929.1 [Q8BM75-1]
RefSeqiNP_076087.2, NM_023598.2 [Q8BM75-1]
UniGeneiMm.440357

Genome annotation databases

EnsembliENSMUST00000218532; ENSMUSP00000151665; ENSMUSG00000019947 [Q8BM75-2]
ENSMUST00000219238; ENSMUSP00000151227; ENSMUSG00000019947 [Q8BM75-1]
GeneIDi71371
KEGGimmu:71371
UCSCiuc007fmf.1 mouse [Q8BM75-2]
uc007fmg.1 mouse [Q8BM75-1]
uc007fmj.1 mouse [Q8BM75-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF280065 mRNA Translation: AAM93269.1
AF401550 mRNA Translation: AAM93282.1
AK017396 mRNA Translation: BAB30727.1
AK031596 mRNA Translation: BAC27467.1
AK034633 mRNA Translation: BAC28778.1 Different initiation.
AC122293 Genomic DNA No translation available.
AC132588 Genomic DNA No translation available.
AF169968 mRNA Translation: AAF89682.1 Sequence problems.
CCDSiCCDS35929.1 [Q8BM75-1]
RefSeqiNP_076087.2, NM_023598.2 [Q8BM75-1]
UniGeneiMm.440357

3D structure databases

ProteinModelPortaliQ8BM75
SMRiQ8BM75
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi214670, 1 interactor
IntActiQ8BM75, 1 interactor
MINTiQ8BM75

PTM databases

iPTMnetiQ8BM75
PhosphoSitePlusiQ8BM75

Proteomic databases

PaxDbiQ8BM75
PeptideAtlasiQ8BM75
PRIDEiQ8BM75

Protocols and materials databases

DNASUi71371
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000218532; ENSMUSP00000151665; ENSMUSG00000019947 [Q8BM75-2]
ENSMUST00000219238; ENSMUSP00000151227; ENSMUSG00000019947 [Q8BM75-1]
GeneIDi71371
KEGGimmu:71371
UCSCiuc007fmf.1 mouse [Q8BM75-2]
uc007fmg.1 mouse [Q8BM75-1]
uc007fmj.1 mouse [Q8BM75-3]

Organism-specific databases

CTDi84159
MGIiMGI:2175912 Arid5b

Phylogenomic databases

eggNOGiENOG410IQ7B Eukaryota
ENOG4110NWK LUCA
GeneTreeiENSGT00900000140901
HOVERGENiHBG050575
InParanoidiQ8BM75
OMAiMTMSAPK
OrthoDBiEOG091G0E6N
TreeFamiTF324725

Enzyme and pathway databases

ReactomeiR-MMU-3214842 HDMs demethylate histones

Miscellaneous databases

ChiTaRSiArid5b mouse
PROiPR:Q8BM75
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000019947 Expressed in 253 organ(s), highest expression level in head
CleanExiMM_ARID5B
ExpressionAtlasiQ8BM75 baseline and differential
GenevisibleiQ8BM75 MM

Family and domain databases

Gene3Di1.10.150.60, 1 hit
InterProiView protein in InterPro
IPR030408 ARID5B
IPR001606 ARID_dom
IPR036431 ARID_dom_sf
PANTHERiPTHR13964:SF37 PTHR13964:SF37, 1 hit
PfamiView protein in Pfam
PF01388 ARID, 1 hit
SMARTiView protein in SMART
SM00501 BRIGHT, 1 hit
SUPFAMiSSF46774 SSF46774, 1 hit
PROSITEiView protein in PROSITE
PS51011 ARID, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiARI5B_MOUSE
AccessioniPrimary (citable) accession number: Q8BM75
Secondary accession number(s): E9Q523
, Q8C0E0, Q8K4G8, Q8K4L9, Q9CU78, Q9JIX4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: June 28, 2011
Last modified: November 7, 2018
This is version 138 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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