UniProtKB - Q8BM75 (ARI5B_MOUSE)
Protein
AT-rich interactive domain-containing protein 5B
Gene
Arid5b
Organism
Mus musculus (Mouse)
Status
Functioni
Transcription coactivator that binds to the 5'-AATA[CT]-3' core sequence and plays a key role in adipogenesis and liver development. Acts by forming a complex with phosphorylated PHF2, which mediates demethylation at Lys-337, leading to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver (By similarity). Required for adipogenesis: regulates triglyceride metabolism in adipocytes by regulating expression of adipogenic genes. Overexpression leads to induction of smooth muscle marker genes, suggesting that it may also act as a regulator of smooth muscle cell differentiation and proliferation.By similarity5 Publications
GO - Molecular functioni
- DNA binding Source: MGI
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: MGI
- RNA polymerase II regulatory region sequence-specific DNA binding Source: MGI
- transcription coactivator activity Source: UniProtKB
- transcription regulatory region DNA binding Source: UniProtKB
GO - Biological processi
- adipose tissue development Source: UniProtKB
- adrenal gland development Source: MGI
- cell development Source: MGI
- cellular response to leukemia inhibitory factor Source: MGI
- face morphogenesis Source: MGI
- fat cell differentiation Source: MGI
- fat pad development Source: MGI
- female gonad development Source: MGI
- fibroblast migration Source: MGI
- kidney development Source: MGI
- male gonad development Source: MGI
- multicellular organism growth Source: MGI
- muscle organ morphogenesis Source: MGI
- negative regulation of transcription by RNA polymerase II Source: MGI
- nitrogen compound metabolic process Source: MGI
- platelet-derived growth factor receptor signaling pathway Source: MGI
- positive regulation of DNA-binding transcription factor activity Source: MGI
- post-embryonic development Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
- roof of mouth development Source: MGI
- skeletal system morphogenesis Source: MGI
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-3214842 HDMs demethylate histones |
Names & Taxonomyi
Protein namesi | Recommended name: AT-rich interactive domain-containing protein 5BShort name: ARID domain-containing protein 5B Alternative name(s): Developmentally and sexually retarded with transient immune abnormalities protein Short name: Desrt MRF1-like Modulator recognition factor protein 2 Short name: MRF-2 |
Gene namesi | Name:Arid5b Synonyms:Desrt, Mrf2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2175912 Arid5b |
Subcellular locationi
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
High rate of neonatal mortality. Embryonic growth or birth weight are not effected, while lipid accumulation is severely reduced in brown adipose neonates at 24 hours of age. Mice weigh significantly less than controls from postnatal day 5 onward. Adult mice are lean, with significant reductions in brown and white adipose tissues, and in the percentage of body fat. Mice are also resistant to weight gains and obesity when maintained on high-fat diets.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000200582 | 1 – 1188 | AT-rich interactive domain-containing protein 5BAdd BLAST | 1188 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 130 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 264 | PhosphoserineBy similarity | 1 | |
Modified residuei | 337 | N6,N6-dimethyllysineBy similarity | 1 | |
Cross-linki | 446 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 494 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 496 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 767 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 774 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 803 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 810 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 893 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 916 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 920 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 935 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 988 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 1000 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 1013 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 1032 | PhosphoserineBy similarity | 1 | |
Cross-linki | 1055 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 1070 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 1133 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Methylation at Lys-337 prevents DNA-binding. Demethylation by PHF2 promotes recruitment of the PHF2-ARID5B complex to promoters (By similarity).By similarity
Keywords - PTMi
Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q8BM75 |
PaxDbi | Q8BM75 |
PeptideAtlasi | Q8BM75 |
PRIDEi | Q8BM75 |
PTM databases
iPTMneti | Q8BM75 |
PhosphoSitePlusi | Q8BM75 |
Expressioni
Tissue specificityi
Widely expressed. Expressed in lung, heart, small intestine, kidney, muscle and brain. Also expressed in spleen, thymus, endocrine organs and in uterus and testis.2 Publications
Developmental stagei
First detected in the intermediate plate mesoderm, and subsequently in the nephrogenic cords of the urogenital ridges. Expressed in the developing limb. Also expressed in the myotome of the somites from 9.5 dpc, the oro-nasopharyngeal ectoderm and underlying mesenchyme, otic vesicles, the gut and its derivatives, and transiently in the liver at 11.5 dpc.1 Publication
Inductioni
During smooth muscle cell differentiation in vitro. Upon adipogenesis.2 Publications
Gene expression databases
Bgeei | ENSMUSG00000019947 Expressed in 253 organ(s), highest expression level in head |
ExpressionAtlasi | Q8BM75 baseline and differential |
Genevisiblei | Q8BM75 MM |
Interactioni
Protein-protein interaction databases
BioGridi | 214670, 1 interactor |
IntActi | Q8BM75, 1 interactor |
MINTi | Q8BM75 |
STRINGi | 10090.ENSMUSP00000020106 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 319 – 411 | ARIDPROSITE-ProRule annotationAdd BLAST | 93 |
Domaini
The ARID domain mediates the interaction with DNA.
Sequence similaritiesi
Belongs to the ARID5B family.Curated
Phylogenomic databases
eggNOGi | ENOG410IQ7B Eukaryota ENOG4110NWK LUCA |
GeneTreei | ENSGT00940000161078 |
InParanoidi | Q8BM75 |
OMAi | DSHPKAC |
OrthoDBi | 368297at2759 |
TreeFami | TF324725 |
Family and domain databases
Gene3Di | 1.10.150.60, 1 hit |
InterProi | View protein in InterPro IPR030408 ARID5B IPR001606 ARID_dom IPR036431 ARID_dom_sf |
PANTHERi | PTHR13964:SF37 PTHR13964:SF37, 1 hit |
Pfami | View protein in Pfam PF01388 ARID, 1 hit |
SMARTi | View protein in SMART SM00501 BRIGHT, 1 hit |
SUPFAMi | SSF46774 SSF46774, 1 hit |
PROSITEi | View protein in PROSITE PS51011 ARID, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8BM75-1) [UniParc]FASTAAdd to basket
Also known as: Mrf2alpha, Alpha
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEPNSLQWVG SPCGLHGPYI FYKAFQFHLE GKPRILSLGD FFFVRCTPKD
60 70 80 90 100
PICIAELQLL WEERTSRQLL SSSKLYFLPE DTPQGRNSDH GEDEVIAVSE
110 120 130 140 150
KVIVKLEDLV KWAHSDFSKW RCGLRATPVK TEAFGRNGQK EALLRYRQST
160 170 180 190 200
LNSGLNFKDV LKEKADLGED EEETNVIVLS YPQYCRYRSM LKRIQDKPSS
210 220 230 240 250
ILTDQFALAL GGIAVVSRNP QILYCRDTFD HPTLIENESV CDEFAPNLKG
260 270 280 290 300
RPRKKKTCPQ RRDSFSGSKD PNNNCDGKVI SKVKGEARSA LTKPKNNHNN
310 320 330 340 350
CKKTSNEEKP KLSIGEECRA DEQAFLVALY KYMKERKTPI ERIPYLGFKQ
360 370 380 390 400
INLWTMFQAA QKLGGYETIT ARRQWKHIYD ELGGNPGSTS AATCTRRHYE
410 420 430 440 450
RLILPYERFI KGEEDKPLPP IKPRKQENNT QENENKTKVS GNKRIKQEMA
460 470 480 490 500
KNKKEKENTP KPQDTSEVSS EQRKEEETLN HKSAPEPLPA PEVKGKPEGH
510 520 530 540 550
KDLGARAPVS RADPEKANET DQGSNSEKEA EEMGDKGLAP LLPSPPLPPE
560 570 580 590 600
KDSAPTPGAG KQPLASPSTQ MDSKQEAKPC CFTESPEKDL QGAPFSSFSA
610 620 630 640 650
TKPPLTSQNE AEEEQLPATA NYIANCTVKV DQLGSDDIHT ALKQTPKVLV
660 670 680 690 700
VQSFDMFKDK DLTGPMNENH GLNYTPLLYS RGNPGIMSPL AKKKLLSQVS
710 720 730 740 750
GASLSSSYPY GSPPPLISKK KLIAREDLCS GLSQGHHSQS SDHTAVSRPS
760 770 780 790 800
VIQHVQSFKN KASEDRKSIN DIFKHDKLSR SDAHRCGFSK HQLGSLADSY
810 820 830 840 850
ILKQETQEGK DKLLEKRAVS HAHVPSFLAD FYSSPHLHSL YRHTEHHLHN
860 870 880 890 900
EQSSKYAARD AYQESENGAF LSHKHPEKIH VNYLASLHLQ DKKVAAAEAS
910 920 930 940 950
TDDQPTDLSL PKNPHKLTSK VLGLAHSTSG SQEIKGASQF QVVSNQSRDC
960 970 980 990 1000
HPKACRVSPM TMSGPKKYPE SLARSGKPHQ VRLENFRKME GMVHPILHRK
1010 1020 1030 1040 1050
MSPQNIGAAR PIKRSLEDLD LVIAGKKARA VSPLDPAKEA SGKEKASEQE
1060 1070 1080 1090 1100
SEGNKGAYGG HSGAASEGHK LPLSTPIFPG LYSGSLCNSG LNSRLPAGYS
1110 1120 1130 1140 1150
HSLQYLKNQT VLSPLMQPLA FHSLVMQRGI FTSPTNSQQL YRHLAAATPV
1160 1170 1180
GSSYGDLLHN SIYPLAGINP QAAFPSSQLS SVHPSTKL
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1X7SB62 | A0A1X7SB62_MOUSE | AT-rich interactive domain-containi... | Arid5b | 254 | Annotation score: |
Sequence cautioni
The sequence AAF89682 differs from that shown. Reason: Frameshift.Curated
The sequence AAF89682 differs from that shown. Reason: Erroneous termination. Truncated C-terminus.Curated
The sequence BAC28778 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 111 | K → T in AAF89682 (PubMed:19468303).Curated | 1 | |
Sequence conflicti | 287 | A → G in AAF89682 (PubMed:19468303).Curated | 1 | |
Sequence conflicti | 474 | K → E in AAM93282 (PubMed:12215486).Curated | 1 | |
Sequence conflicti | 474 | K → E in AAM93269 (PubMed:12215486).Curated | 1 | |
Sequence conflicti | 474 | K → E in AAF89682 (PubMed:19468303).Curated | 1 | |
Sequence conflicti | 480 | N → S in AAM93282 (PubMed:12215486).Curated | 1 | |
Sequence conflicti | 480 | N → S in AAM93269 (PubMed:12215486).Curated | 1 | |
Sequence conflicti | 480 | N → S in AAF89682 (PubMed:19468303).Curated | 1 | |
Sequence conflicti | 928 | T → A in AAM93282 (PubMed:12215486).Curated | 1 | |
Sequence conflicti | 928 | T → A in AAM93269 (PubMed:12215486).Curated | 1 | |
Sequence conflicti | 1056 | G → S in AAM93282 (PubMed:12215486).Curated | 1 | |
Sequence conflicti | 1056 | G → S in AAM93269 (PubMed:12215486).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_009357 | 1 – 243 | Missing in isoform 2. 1 PublicationAdd BLAST | 243 | |
Alternative sequenceiVSP_009358 | 244 | F → M in isoform 2. 1 Publication | 1 | |
Alternative sequenceiVSP_009359 | 245 – 276 | APNLK…NNNCD → GECFSFTLFETRPNESLFSI HGVSSAGEQGLV in isoform 3. 1 PublicationAdd BLAST | 32 | |
Alternative sequenceiVSP_009360 | 277 – 1188 | Missing in isoform 3. 1 PublicationAdd BLAST | 912 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF280065 mRNA Translation: AAM93269.1 AF401550 mRNA Translation: AAM93282.1 AK017396 mRNA Translation: BAB30727.1 AK031596 mRNA Translation: BAC27467.1 AK034633 mRNA Translation: BAC28778.1 Different initiation. AC122293 Genomic DNA No translation available. AC132588 Genomic DNA No translation available. AF169968 mRNA Translation: AAF89682.1 Sequence problems. |
CCDSi | CCDS35929.1 [Q8BM75-1] |
RefSeqi | NP_076087.2, NM_023598.2 [Q8BM75-1] |
Genome annotation databases
Ensembli | ENSMUST00000218532; ENSMUSP00000151665; ENSMUSG00000019947 [Q8BM75-2] ENSMUST00000219238; ENSMUSP00000151227; ENSMUSG00000019947 [Q8BM75-1] |
GeneIDi | 71371 |
KEGGi | mmu:71371 |
UCSCi | uc007fmf.1 mouse [Q8BM75-2] uc007fmg.1 mouse [Q8BM75-1] uc007fmj.1 mouse [Q8BM75-3] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF280065 mRNA Translation: AAM93269.1 AF401550 mRNA Translation: AAM93282.1 AK017396 mRNA Translation: BAB30727.1 AK031596 mRNA Translation: BAC27467.1 AK034633 mRNA Translation: BAC28778.1 Different initiation. AC122293 Genomic DNA No translation available. AC132588 Genomic DNA No translation available. AF169968 mRNA Translation: AAF89682.1 Sequence problems. |
CCDSi | CCDS35929.1 [Q8BM75-1] |
RefSeqi | NP_076087.2, NM_023598.2 [Q8BM75-1] |
3D structure databases
SMRi | Q8BM75 |
ModBasei | Search... |
Protein-protein interaction databases
BioGridi | 214670, 1 interactor |
IntActi | Q8BM75, 1 interactor |
MINTi | Q8BM75 |
STRINGi | 10090.ENSMUSP00000020106 |
PTM databases
iPTMneti | Q8BM75 |
PhosphoSitePlusi | Q8BM75 |
Proteomic databases
EPDi | Q8BM75 |
PaxDbi | Q8BM75 |
PeptideAtlasi | Q8BM75 |
PRIDEi | Q8BM75 |
Protocols and materials databases
DNASUi | 71371 |
Genome annotation databases
Ensembli | ENSMUST00000218532; ENSMUSP00000151665; ENSMUSG00000019947 [Q8BM75-2] ENSMUST00000219238; ENSMUSP00000151227; ENSMUSG00000019947 [Q8BM75-1] |
GeneIDi | 71371 |
KEGGi | mmu:71371 |
UCSCi | uc007fmf.1 mouse [Q8BM75-2] uc007fmg.1 mouse [Q8BM75-1] uc007fmj.1 mouse [Q8BM75-3] |
Organism-specific databases
CTDi | 84159 |
MGIi | MGI:2175912 Arid5b |
Phylogenomic databases
eggNOGi | ENOG410IQ7B Eukaryota ENOG4110NWK LUCA |
GeneTreei | ENSGT00940000161078 |
InParanoidi | Q8BM75 |
OMAi | DSHPKAC |
OrthoDBi | 368297at2759 |
TreeFami | TF324725 |
Enzyme and pathway databases
Reactomei | R-MMU-3214842 HDMs demethylate histones |
Miscellaneous databases
ChiTaRSi | Arid5b mouse |
PROi | PR:Q8BM75 |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000019947 Expressed in 253 organ(s), highest expression level in head |
ExpressionAtlasi | Q8BM75 baseline and differential |
Genevisiblei | Q8BM75 MM |
Family and domain databases
Gene3Di | 1.10.150.60, 1 hit |
InterProi | View protein in InterPro IPR030408 ARID5B IPR001606 ARID_dom IPR036431 ARID_dom_sf |
PANTHERi | PTHR13964:SF37 PTHR13964:SF37, 1 hit |
Pfami | View protein in Pfam PF01388 ARID, 1 hit |
SMARTi | View protein in SMART SM00501 BRIGHT, 1 hit |
SUPFAMi | SSF46774 SSF46774, 1 hit |
PROSITEi | View protein in PROSITE PS51011 ARID, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ARI5B_MOUSE | |
Accessioni | Q8BM75Primary (citable) accession number: Q8BM75 Secondary accession number(s): E9Q523 Q9JIX4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 2, 2004 |
Last sequence update: | June 28, 2011 | |
Last modified: | October 16, 2019 | |
This is version 145 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot