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UniProtKB - Q8BLM0 (KLF8_MOUSE)
Protein
Krueppel-like factor 8
Gene
Klf8
Organism
Mus musculus (Mouse)
Status
Functioni
Transcriptional repressor and activator. Binds to CACCC-boxes promoter elements. Also binds the GT-box of cyclin D1 promoter and mediates cell cycle progression at G1 phase as a downstream target of focal adhesion kinase (FAK) (By similarity).
By similarityRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 270 – 294 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 300 – 324 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 330 – 352 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 |
GO - Molecular functioni
- DNA binding Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: MGI
- metal ion binding Source: UniProtKB-KW
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: MGI
GO - Biological processi
- negative regulation of transcription by RNA polymerase II Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Krueppel-like factor 8 |
Gene namesi | Name:Klf8 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2442430, Klf8 |
VEuPathDBi | HostDB:ENSMUSG00000041649 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Cytosol
- cytosol Source: MGI
Nucleus
- nucleoplasm Source: MGI
Other locations
- aggresome Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000294168 | 1 – 355 | Krueppel-like factor 8Add BLAST | 355 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 64 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity |
Post-translational modificationi
Sumoylation at Lys-64 represses transcriptional activity and reduces cell cycle progression into the G1 phase. Has no effect on subcellular location.
Keywords - PTMi
Isopeptide bond, Ubl conjugationProteomic databases
PaxDbi | Q8BLM0 |
PeptideAtlasi | Q8BLM0 |
PRIDEi | Q8BLM0 |
ProteomicsDBi | 264998 |
PTM databases
iPTMneti | Q8BLM0 |
PhosphoSitePlusi | Q8BLM0 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000041649, Expressed in telencephalon and 73 other tissues |
ExpressionAtlasi | Q8BLM0, baseline and differential |
Interactioni
Subunit structurei
Interacts with corepressor CtBP2.
Interacts with PIAS1, PIAS2, AND PIAS4; the interaction with each ligase sumoylates KLF8 (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 10090.ENSMUSP00000108193 |
Miscellaneous databases
RNActi | Q8BLM0, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 19 – 52 | DisorderedSequence analysisAdd BLAST | 34 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 82 – 90 | 9aaTAD; inactiveBy similarity | 9 |
Domaini
The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. In KLF8, the motif is inactive.By similarity
Sequence similaritiesi
Belongs to the Sp1 C2H2-type zinc-finger protein family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 270 – 294 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 300 – 324 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 330 – 352 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000161062 |
HOGENOMi | CLU_002678_33_0_1 |
InParanoidi | Q8BLM0 |
OMAi | AMTQMQG |
OrthoDBi | 1318335at2759 |
PhylomeDBi | Q8BLM0 |
TreeFami | TF350556 |
Family and domain databases
InterProi | View protein in InterPro IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00096, zf-C2H2, 3 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 3 hits |
SUPFAMi | SSF57667, SSF57667, 2 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 3 hits PS50157, ZINC_FINGER_C2H2_2, 3 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q8BLM0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDKFIDNMDV RIKSESGSMQ VFKQVTGPVP TRDPSARADR RNMTSPSFLA
60 70 80 90 100
ASPMENPALF NDIKIEPPEE LLESDFNMPQ VEPVDLSFHK PKAPLQPASM
110 120 130 140 150
LQAPIRPPKP PTAPQAIMVP TSADTVTSAA IPTVLTPGSI LASSQGTGGQ
160 170 180 190 200
PILHVIHTIP SVSLPNKMSG LKTIPLVVQS LPMVYTSLPT DGSPAAITVP
210 220 230 240 250
LIGGDGKSAG SVKVDPASMC PLEFPSDSDE SAIESGSSAL QSLQGFHHEP
260 270 280 290 300
ATMVHMQGEE SLDLKRRRIH QCDFAGCSKV YTKSSHLKAH RRIHTGEKPY
310 320 330 340 350
KCTWDGCSWK FARSDELTRH FRKHTGIKPF RCTDCNRSFS RSDHLSLHRR
RHDTM
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA6PWN1 | A6PWN1_MOUSE | Krueppel-like factor 8 | Klf8 | 19 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK044185 mRNA Translation: BAC31807.1 BC070442 mRNA Translation: AAH70442.1 |
CCDSi | CCDS30481.1 |
RefSeqi | NP_776141.1, NM_173780.4 XP_006528916.1, XM_006528853.3 XP_006528921.1, XM_006528858.3 XP_006528922.1, XM_006528859.3 XP_006528923.1, XM_006528860.3 XP_011246123.1, XM_011247821.2 XP_017173992.1, XM_017318503.1 XP_017173993.1, XM_017318504.1 XP_017173994.1, XM_017318505.1 XP_017173995.1, XM_017318506.1 |
Genome annotation databases
Ensembli | ENSMUST00000039545; ENSMUSP00000044317; ENSMUSG00000041649 ENSMUST00000112574; ENSMUSP00000108193; ENSMUSG00000041649 |
GeneIDi | 245671 |
KEGGi | mmu:245671 |
UCSCi | uc009uqu.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK044185 mRNA Translation: BAC31807.1 BC070442 mRNA Translation: AAH70442.1 |
CCDSi | CCDS30481.1 |
RefSeqi | NP_776141.1, NM_173780.4 XP_006528916.1, XM_006528853.3 XP_006528921.1, XM_006528858.3 XP_006528922.1, XM_006528859.3 XP_006528923.1, XM_006528860.3 XP_011246123.1, XM_011247821.2 XP_017173992.1, XM_017318503.1 XP_017173993.1, XM_017318504.1 XP_017173994.1, XM_017318505.1 XP_017173995.1, XM_017318506.1 |
3D structure databases
SMRi | Q8BLM0 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000108193 |
PTM databases
iPTMneti | Q8BLM0 |
PhosphoSitePlusi | Q8BLM0 |
Proteomic databases
PaxDbi | Q8BLM0 |
PeptideAtlasi | Q8BLM0 |
PRIDEi | Q8BLM0 |
ProteomicsDBi | 264998 |
Protocols and materials databases
Antibodypediai | 13002, 224 antibodies from 32 providers |
DNASUi | 245671 |
Genome annotation databases
Ensembli | ENSMUST00000039545; ENSMUSP00000044317; ENSMUSG00000041649 ENSMUST00000112574; ENSMUSP00000108193; ENSMUSG00000041649 |
GeneIDi | 245671 |
KEGGi | mmu:245671 |
UCSCi | uc009uqu.1, mouse |
Organism-specific databases
CTDi | 11279 |
MGIi | MGI:2442430, Klf8 |
VEuPathDBi | HostDB:ENSMUSG00000041649 |
Phylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000161062 |
HOGENOMi | CLU_002678_33_0_1 |
InParanoidi | Q8BLM0 |
OMAi | AMTQMQG |
OrthoDBi | 1318335at2759 |
PhylomeDBi | Q8BLM0 |
TreeFami | TF350556 |
Miscellaneous databases
BioGRID-ORCSi | 245671, 0 hits in 64 CRISPR screens |
ChiTaRSi | Klf8, mouse |
PROi | PR:Q8BLM0 |
RNActi | Q8BLM0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000041649, Expressed in telencephalon and 73 other tissues |
ExpressionAtlasi | Q8BLM0, baseline and differential |
Family and domain databases
InterProi | View protein in InterPro IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00096, zf-C2H2, 3 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 3 hits |
SUPFAMi | SSF57667, SSF57667, 2 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 3 hits PS50157, ZINC_FINGER_C2H2_2, 3 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | KLF8_MOUSE | |
Accessioni | Q8BLM0Primary (citable) accession number: Q8BLM0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 10, 2007 |
Last sequence update: | March 1, 2003 | |
Last modified: | February 23, 2022 | |
This is version 150 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families