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Protein

Transient receptor potential cation channel subfamily A member 1

Gene

Trpa1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Receptor-activated non-selective cation channel involved in detection of pain and possibly also in cold perception and inner ear function. Has a central role in the pain response to endogenous inflammatory mediators and to a diverse array of volatile irritants, such as mustard oil, cinnamaldehyde, garlic and acrolein, an irritant from tears gas and vehicule exhaust fumes. Acts also as a ionotropic cannabinoid receptor by being activated by delta9-tetrahydrocannabinol (THC), the psychoactive component of marijuana. Is also activated by menthol (in vitro) (By similarity). May be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds. Probably operated by a phosphatidylinositol second messenger system.By similarity5 Publications

Activity regulationi

Inhibited by ruthenium red, a potent blocker of TRPV channels.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel
Biological processIon transport, Sensory transduction, Transport

Enzyme and pathway databases

ReactomeiR-MMU-3295583 TRP channels

Protein family/group databases

TCDBi1.A.4.6.1 the transient receptor potential ca(2+) channel (trp-cc) family

Names & Taxonomyi

Protein namesi
Recommended name:
Transient receptor potential cation channel subfamily A member 1
Alternative name(s):
Ankyrin-like with transmembrane domains protein 1
Gene namesi
Name:Trpa1
Synonyms:Anktm11 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:3522699 Trpa1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 721CytoplasmicBy similarityAdd BLAST721
Transmembranei722 – 742Helical; Name=1By similarityAdd BLAST21
Topological domaini743 – 767ExtracellularBy similarityAdd BLAST25
Transmembranei768 – 788Helical; Name=2By similarityAdd BLAST21
Topological domaini789 – 806CytoplasmicBy similarityAdd BLAST18
Transmembranei807 – 827Helical; Name=3By similarityAdd BLAST21
Topological domaini828 – 832ExtracellularBy similarity5
Transmembranei833 – 853Helical; Name=4By similarityAdd BLAST21
Topological domaini854 – 876CytoplasmicBy similarityAdd BLAST23
Transmembranei877 – 897Helical; Name=5Sequence analysisBy similarityAdd BLAST21
Topological domaini898 – 904ExtracellularBy similarity7
Intramembranei905 – 925Pore-formingBy similarityAdd BLAST21
Topological domaini926 – 937ExtracellularBy similarityAdd BLAST12
Transmembranei938 – 959Helical; Name=6By similarityAdd BLAST22
Topological domaini960 – 1125CytoplasmicBy similarityAdd BLAST166

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice display normal cold sensitivity and unimpaired auditory function, suggesting that this channel is not required for the initial detection of noxious cold or sound. However, they exhibit pronounced deficits in bradykinin-evoked nociceptor excitation and pain hypersensitivity.1 Publication

Chemistry databases

ChEMBLiCHEMBL1075310
GuidetoPHARMACOLOGYi485

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002153701 – 1125Transient receptor potential cation channel subfamily A member 1Add BLAST1125

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi193 ↔ 666Alternate1 Publication
Disulfide bondi463 ↔ 666Alternate1 Publication
Disulfide bondi609 ↔ 622Alternate1 Publication
Disulfide bondi622 ↔ 666Alternate1 Publication
Glycosylationi749N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi755N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

TRPA1 activation by electrophiles occurs though covalent modification of specific cysteine residues in the N-terminal cytoplasmic domain.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8BLA8
PRIDEiQ8BLA8

PTM databases

PhosphoSitePlusiQ8BLA8

Expressioni

Tissue specificityi

Expressed in inner ear (at protein level). Specifically expressed in a subset of nociceptive neurons. Expressed in the same neurons that TRPV1. In contrast, it is not expressed in neurons expressing TRPM8. Expressed in the superior cervical ganglion.4 Publications

Developmental stagei

During utricle development, it is first expressed at E15 and E16 and peaks at E17. It drops at E18 but increases again at E19, possibly corresponding to a second wave of hair cells that are generated at E15.1 Publication

Gene expression databases

BgeeiENSMUSG00000032769 Expressed in 29 organ(s), highest expression level in embryo
GenevisibleiQ8BLA8 MM

Interactioni

Subunit structurei

Homotetramer (PubMed:21908607). Interacts with TMEM100 (PubMed:25640077).2 Publications

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-61521N
STRINGi10090.ENSMUSP00000043594

Chemistry databases

BindingDBiQ8BLA8

Structurei

3D structure databases

ProteinModelPortaliQ8BLA8
SMRiQ8BLA8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati63 – 94ANK 1Add BLAST32
Repeati98 – 127ANK 2Add BLAST30
Repeati131 – 161ANK 3Add BLAST31
Repeati165 – 194ANK 4Add BLAST30
Repeati198 – 227ANK 5Add BLAST30
Repeati239 – 268ANK 6Add BLAST30
Repeati272 – 301ANK 7Add BLAST30
Repeati309 – 338ANK 8Add BLAST30
Repeati342 – 371ANK 9Add BLAST30
Repeati413 – 442ANK 10Add BLAST30
Repeati446 – 475ANK 11Add BLAST30
Repeati482 – 511ANK 12Add BLAST30
Repeati514 – 543ANK 13Add BLAST30
Repeati548 – 577ANK 14Add BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1048 – 1054Inositolphosphate bindingBy similarity7

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1044 – 1073By similarityAdd BLAST30

Domaini

C-terminal helices from the four subunits associate to form atypical coiled coil structure; this region is probably involved in binding the inositol polyphosphates that are required for optimal channel activity (in vitro).By similarity
The ANK repeat domain consists of a convex stem structure followed by a crescent-shaped structure that surrounds the protein core.By similarity

Sequence similaritiesi

Keywords - Domaini

ANK repeat, Coiled coil, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0510 Eukaryota
COG0666 LUCA
GeneTreeiENSGT00920000149074
HOGENOMiHOG000044486
HOVERGENiHBG059027
InParanoidiQ8BLA8
KOiK04984
OMAiYYIEYNF
OrthoDBiEOG091G01BG
PhylomeDBiQ8BLA8
TreeFamiTF317264

Family and domain databases

CDDicd00204 ANK, 4 hits
Gene3Di1.25.40.20, 3 hits
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR005821 Ion_trans_dom
IPR030288 TRPA1/painless
PANTHERiPTHR24190 PTHR24190, 1 hit
PfamiView protein in Pfam
PF12796 Ank_2, 5 hits
PF00520 Ion_trans, 1 hit
PRINTSiPR01415 ANKYRIN
SMARTiView protein in SMART
SM00248 ANK, 14 hits
SUPFAMiSSF48403 SSF48403, 2 hits
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 9 hits

Sequencei

Sequence statusi: Complete.

Q8BLA8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKRGLRRILL PEERKEVQGV VYRGVGEDMD CSKESFKVDI EGDMCRLEDF
60 70 80 90 100
IKNRRKLSKY EDENLCPLHH AAAEGQVELM ELIINGSSCE VLNIMDGYGN
110 120 130 140 150
TPLHCAAEKN QVESVKFLLS QGANPNLRNR NMMSPLHIAV HGMYNEVIKV
160 170 180 190 200
LTEHKATNIN LEGENGNTAL MSTCAKDNSE ALQILLEKGA KLCKSNKWGD
210 220 230 240 250
YPVHQAAFSG AKKCMELILA YGEKNGYSRE THINFVNHKK ASPLHLAVQS
260 270 280 290 300
GDLDMIKMCL DNGAHIDMME NAKCMALHFA ATQGATDIVK LMISSYTGSS
310 320 330 340 350
DIVNAVDGNQ ETLLHRASLF DHHDLAEYLI SVGADINSTD SEGRSPLILA
360 370 380 390 400
TASASWNIVN LLLCKGAKVD IKDHLGRNFL HLTVQQPYGL RNLRPEFMQM
410 420 430 440 450
QHIKELVMDE DNDGCTPLHY ACRQGVPVSV NNLLGFNVSI HSKSKDKKSP
460 470 480 490 500
LHFAASYGRI NTCQRLLQDI SDTRLLNEGD LHGMTPLHLA AKNGHDKVVQ
510 520 530 540 550
LLLKKGALFL SDHNGWTALH HASMGGYTQT MKVILDTNLK CTDRLDEEGN
560 570 580 590 600
TALHFAAREG HAKAVAMLLS YNADILLNKK QASFLHIALH NKRKEVVLTT
610 620 630 640 650
IRNKRWDECL QVFTHNSPSN RCPIMEMVEY LPECMKVLLD FCMIPSTEDK
660 670 680 690 700
SCQDYHIEYN FKYLQCPLSM TKKVAPTQDV VYEPLTILNV MVQHNRIELL
710 720 730 740 750
NHPVCREYLL MKWCAYGFRA HMMNLGSYCL GLIPMTLLVV KIQPGMAFNS
760 770 780 790 800
TGIINGTSST HEERIDTLNS FPIKICMILV FLSSIFGYCK EVIQIFQQKR
810 820 830 840 850
NYFLDYNNAL EWVIYTTSII FVLPLFLNIP AYMQWQCGAI AIFFYWMNFL
860 870 880 890 900
LYLQRFENCG IFIVMLEVIF KTLLRSTGVF IFLLLAFGLS FYVLLNFQDA
910 920 930 940 950
FSTPLLSLIQ TFSMMLGDIN YRDAFLEPLF RNELAYPVLT FGQLIAFTMF
960 970 980 990 1000
VPIVLMNLLI GLAVGDIAEV QKHASLKRIA MQVELHTNLE KKLPLWYLRK
1010 1020 1030 1040 1050
VDQRSTIVYP NRPRHGRMLR FFHYFLNMQE TRQEVPNIDT CLEMEILKQK
1060 1070 1080 1090 1100
YRLKDLTSLL EKQHELIKLI IQKMEIISET EDEDNHCSFQ DRFKKERLEQ
1110 1120
MHSKWNFVLN AVKTKTHCSI SHPDF
Length:1,125
Mass (Da):128,467
Last modified:March 1, 2003 - v1
Checksum:iCD718BF3ED4AC291
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY231177 mRNA Translation: AAO43183.1
AK045771 mRNA Translation: BAC32487.1
BC120563 mRNA Translation: AAI20564.1
BC131963 mRNA Translation: AAI31964.1
CCDSiCCDS35516.1
RefSeqiNP_001335217.1, NM_001348288.1
NP_808449.1, NM_177781.5
UniGeneiMm.186329

Genome annotation databases

EnsembliENSMUST00000041447; ENSMUSP00000043594; ENSMUSG00000032769
GeneIDi277328
KEGGimmu:277328
UCSCiuc007ajc.1 mouse

Similar proteinsi

Cross-referencesi

Web resourcesi

Protein Spotlight

The power behind pain - Issue 82 of May 2007

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY231177 mRNA Translation: AAO43183.1
AK045771 mRNA Translation: BAC32487.1
BC120563 mRNA Translation: AAI20564.1
BC131963 mRNA Translation: AAI31964.1
CCDSiCCDS35516.1
RefSeqiNP_001335217.1, NM_001348288.1
NP_808449.1, NM_177781.5
UniGeneiMm.186329

3D structure databases

ProteinModelPortaliQ8BLA8
SMRiQ8BLA8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-61521N
STRINGi10090.ENSMUSP00000043594

Chemistry databases

BindingDBiQ8BLA8
ChEMBLiCHEMBL1075310
GuidetoPHARMACOLOGYi485

Protein family/group databases

TCDBi1.A.4.6.1 the transient receptor potential ca(2+) channel (trp-cc) family

PTM databases

PhosphoSitePlusiQ8BLA8

Proteomic databases

PaxDbiQ8BLA8
PRIDEiQ8BLA8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000041447; ENSMUSP00000043594; ENSMUSG00000032769
GeneIDi277328
KEGGimmu:277328
UCSCiuc007ajc.1 mouse

Organism-specific databases

CTDi8989
MGIiMGI:3522699 Trpa1

Phylogenomic databases

eggNOGiKOG0510 Eukaryota
COG0666 LUCA
GeneTreeiENSGT00920000149074
HOGENOMiHOG000044486
HOVERGENiHBG059027
InParanoidiQ8BLA8
KOiK04984
OMAiYYIEYNF
OrthoDBiEOG091G01BG
PhylomeDBiQ8BLA8
TreeFamiTF317264

Enzyme and pathway databases

ReactomeiR-MMU-3295583 TRP channels

Miscellaneous databases

PROiPR:Q8BLA8
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032769 Expressed in 29 organ(s), highest expression level in embryo
GenevisibleiQ8BLA8 MM

Family and domain databases

CDDicd00204 ANK, 4 hits
Gene3Di1.25.40.20, 3 hits
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR005821 Ion_trans_dom
IPR030288 TRPA1/painless
PANTHERiPTHR24190 PTHR24190, 1 hit
PfamiView protein in Pfam
PF12796 Ank_2, 5 hits
PF00520 Ion_trans, 1 hit
PRINTSiPR01415 ANKYRIN
SMARTiView protein in SMART
SM00248 ANK, 14 hits
SUPFAMiSSF48403 SSF48403, 2 hits
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 9 hits
ProtoNetiSearch...

Entry informationi

Entry nameiTRPA1_MOUSE
AccessioniPrimary (citable) accession number: Q8BLA8
Secondary accession number(s): Q0VBN5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: March 1, 2003
Last modified: September 12, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
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Main funding by: National Institutes of Health

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