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UniProtKB - Q8BL66 (EEA1_MOUSE)
Protein
Early endosome antigen 1
Gene
Eea1
Organism
Mus musculus (Mouse)
Status
Functioni
Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
By similaritySites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 1358 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 1361 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 1374 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 1377 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 1382 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 1385 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 1402 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 1405 | Zinc 2PROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 41 – 64 | C2H2-typePROSITE-ProRule annotationAdd BLAST | 24 | |
Zinc fingeri | 1352 – 1410 | FYVE-typePROSITE-ProRule annotationAdd BLAST | 59 |
GO - Molecular functioni
- 1-phosphatidylinositol binding Source: UniProtKB
- GTP-dependent protein binding Source: MGI
- metal ion binding Source: UniProtKB-KW
- protein homodimerization activity Source: MGI
GO - Biological processi
- endocytosis Source: UniProtKB
- vesicle fusion Source: MGI
- viral RNA genome replication Source: MGI
Keywordsi
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-168138, Toll Like Receptor 9 (TLR9) Cascade |
Names & Taxonomyi
Protein namesi | Recommended name: Early endosome antigen 1 |
Gene namesi | Name:Eea1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2442192, Eea1 |
VEuPathDBi | HostDB:ENSMUSG00000036499 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Endosome
- Early endosome membrane By similarity; Peripheral membrane protein By similarity
Cytosol
- cytosol Source: UniProtKB
Endosome
- early endosome Source: MGI
- early endosome membrane Source: UniProtKB-SubCell
- endosome Source: MGI
- recycling endosome Source: MGI
Plasma Membrane
- extrinsic component of plasma membrane Source: UniProtKB
Other locations
- axonal spine Source: MGI
- cytoplasmic vesicle Source: MGI
- serine-pyruvate aminotransferase complex Source: MGI
Keywords - Cellular componenti
Cytoplasm, Endosome, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000098707 | 1 – 1411 | Early endosome antigen 1Add BLAST | 1411 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 52 | PhosphoserineBy similarity | 1 | |
Modified residuei | 70 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q8BL66 |
jPOSTi | Q8BL66 |
MaxQBi | Q8BL66 |
PaxDbi | Q8BL66 |
PeptideAtlasi | Q8BL66 |
PRIDEi | Q8BL66 |
ProteomicsDBi | 275903 |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00453776 |
PTM databases
iPTMneti | Q8BL66 |
PhosphoSitePlusi | Q8BL66 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000036499, Expressed in secondary oocyte and 307 other tissues |
Genevisiblei | Q8BL66, MM |
Interactioni
Subunit structurei
Homodimer. Binds STX6. Binds RAB5A, RAB5B, RAB5C and RAB22A that have been activated by GTP-binding.
Interacts with ERBB2 (By similarity).
Interacts with RAB31.
Interacts with SAMD9 AND SAMD9L (By similarity). May interact with PLEKHF2 (By similarity).
By similarityGO - Molecular functioni
- GTP-dependent protein binding Source: MGI
- protein homodimerization activity Source: MGI
Protein-protein interaction databases
BioGRIDi | 229728, 36 interactors |
IntActi | Q8BL66, 29 interactors |
MINTi | Q8BL66 |
STRINGi | 10090.ENSMUSP00000061493 |
Miscellaneous databases
RNActi | Q8BL66, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 27 | DisorderedSequence analysisAdd BLAST | 27 | |
Regioni | 476 – 501 | DisorderedSequence analysisAdd BLAST | 26 | |
Regioni | 1189 – 1217 | DisorderedSequence analysisAdd BLAST | 29 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 78 – 1348 | Sequence analysisAdd BLAST | 1271 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 11 – 27 | Polar residuesSequence analysisAdd BLAST | 17 |
Domaini
The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns3P-enriched membranes is substantially increased in acidic conditions (By similarity).By similarity
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 41 – 64 | C2H2-typePROSITE-ProRule annotationAdd BLAST | 24 | |
Zinc fingeri | 1352 – 1410 | FYVE-typePROSITE-ProRule annotationAdd BLAST | 59 |
Keywords - Domaini
Coiled coil, Zinc-fingerPhylogenomic databases
eggNOGi | ENOG502QWB5, Eukaryota |
GeneTreei | ENSGT00940000156910 |
HOGENOMi | CLU_256550_0_0_1 |
InParanoidi | Q8BL66 |
OMAi | FIAVYQH |
OrthoDBi | 753155at2759 |
PhylomeDBi | Q8BL66 |
TreeFami | TF329698 |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR013087, Znf_C2H2_type IPR000306, Znf_FYVE IPR017455, Znf_FYVE-rel IPR011011, Znf_FYVE_PHD IPR013083, Znf_RING/FYVE/PHD |
Pfami | View protein in Pfam PF01363, FYVE, 1 hit |
SMARTi | View protein in SMART SM00064, FYVE, 1 hit |
SUPFAMi | SSF57903, SSF57903, 1 hit |
PROSITEi | View protein in PROSITE PS50178, ZF_FYVE, 1 hit PS00028, ZINC_FINGER_C2H2_1, 1 hit PS50157, ZINC_FINGER_C2H2_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q8BL66-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFRRILQRTP GRVGSQGSDL DSSATPINTV DVNNESSSEG FICPQCMKSL
60 70 80 90 100
GSADELFKHY QAVHDAGNDS GHGGEAGLAL TRDDITLLRQ EVQDLQASLK
110 120 130 140 150
EEKWYSEELK KELEKYQGLQ QQEAKSDGLV TDSSAELQAL EQQLEEAQTE
160 170 180 190 200
NFNIKQMKDL FEQKAAQLAT EIADIKSKYD EEKSLRAAAE QKVTHLTEDL
210 220 230 240 250
NKQTTVIQDL KTELLQRPGI EDVAVLKKEL VQVQTLMDNM TLERERESEK
260 270 280 290 300
LKDECKKLQS EHAHLEATIN QLRSELAKGP QEVAVYVQEI QKLKGSINEL
310 320 330 340 350
TQKNQNLTEK LQKKDLDYTH LEEKHNEESA SRKTLQASLH QRDLDCQQLQ
360 370 380 390 400
ARLTASESSL QRAQGELSEK AEAAQKLREE LREVESTRQH LKVEVKQLQQ
410 420 430 440 450
QREEKEQHGL QLQGEVSQLH CKLLETERQL GEAHGRLKEQ RQLSSEKLME
460 470 480 490 500
KEQQVADLQL KLSRLEEQLK EKVTNSTELQ HQLEKSKQQH QEQQALQQSA
510 520 530 540 550
TAKLREAQND LEQVLRQIGD KDQKIQNLEA LLQKGKESVS LLEKEREDLY
560 570 580 590 600
AKIQAGEGET AVLNQLQEKN HALQQQLTQL TEKLKNQSES HKQAEENLHD
610 620 630 640 650
QVQEQKAHLR AAQDRVLSLE TSVSELSSQL NESKEKVSQL DIQIKAKTEL
660 670 680 690 700
LLSAEAAKAA QRADLQNHLD TAQHALQDKQ QELNKVSVQL DQLTAKFQEK
710 720 730 740 750
QEHCIQLESH LKDHKEKHLS LEQKVEDLEG HIKKLEADAL EVKASKEQAL
760 770 780 790 800
QSLQQQRQLS TDLELRNAEL SRELQEQEEV VSCTKLDLQN KSEILENIKQ
810 820 830 840 850
TLTKKEEENV VLKQEFEKLS QDSKTQHKEL GDRMQAAVTE LTAVKAQKDA
860 870 880 890 900
LLAELSTTKE KLSKVSDSLK NSKSEFEKEN QKGKAAVLDL EKACKELKHQ
910 920 930 940 950
LQVQAESALK EQEDLKKSLE KEKETSQQLK IELNSVKGEV SQAQNTLKQK
960 970 980 990 1000
EKDEQQLQGT INQLKQSAEQ KKKQIEALQG EVKNAVSQKT VLENKLQQQS
1010 1020 1030 1040 1050
SQAAQELAAE KGKLSALQSN YEKCQADLKQ LQSDLYGKES ELLATRQDLK
1060 1070 1080 1090 1100
SVEEKLTLAQ EDLISNRNQI GNQNKSIQEL QAAKASLEQD SAKKEALLKE
1110 1120 1130 1140 1150
QSKALEDAQR EKSVKEKELV AEKSKLAEME EIKCRQEKEI TKLNEELKSH
1160 1170 1180 1190 1200
KQESIKEITN LKDAKQLLIQ QKLELQGRVD SLKAALEQEK ESQQLMREQV
1210 1220 1230 1240 1250
KKEEEKRKEE FSEKEAKLHS EIKEKEAGMK KHEENEAKLT MQVTTLNENL
1260 1270 1280 1290 1300
GTVKKEWQSS QRRVSELEKQ TDDLRGEIAV LEATVQNNQD ERRALLERCL
1310 1320 1330 1340 1350
KGEGEIEKLQ TKALELQRKL DNTTAAVQEL GRENQSLQIK HTQALNRKWA
1360 1370 1380 1390 1400
EDNEVQNCMS CGKCFSVTVR RHHCRQCGNI FCAECSTKNA LTPSSKKPVR
1410
VCDACFNDLQ G
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A1W2P7A6 | A0A1W2P7A6_MOUSE | Early endosome antigen 1 | Eea1 | 131 | Annotation score: | ||
A0A1W2P7K1 | A0A1W2P7K1_MOUSE | Early endosome antigen 1 | Eea1 | 40 | Annotation score: |
Sequence cautioni
The sequence BAC32647 differs from that shown. Reason: Erroneous termination. Truncated C-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 225 | V → I in BAC32647 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC075637 mRNA Translation: AAH75637.1 AK046231 mRNA Translation: BAC32647.1 Sequence problems. |
CCDSi | CCDS36042.1 |
RefSeqi | NP_001001932.1, NM_001001932.3 |
Genome annotation databases
Ensembli | ENSMUST00000053484; ENSMUSP00000061493; ENSMUSG00000036499 |
GeneIDi | 216238 |
KEGGi | mmu:216238 |
UCSCi | uc007gwu.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC075637 mRNA Translation: AAH75637.1 AK046231 mRNA Translation: BAC32647.1 Sequence problems. |
CCDSi | CCDS36042.1 |
RefSeqi | NP_001001932.1, NM_001001932.3 |
3D structure databases
AlphaFoldDBi | Q8BL66 |
SMRi | Q8BL66 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 229728, 36 interactors |
IntActi | Q8BL66, 29 interactors |
MINTi | Q8BL66 |
STRINGi | 10090.ENSMUSP00000061493 |
PTM databases
iPTMneti | Q8BL66 |
PhosphoSitePlusi | Q8BL66 |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00453776 |
Proteomic databases
EPDi | Q8BL66 |
jPOSTi | Q8BL66 |
MaxQBi | Q8BL66 |
PaxDbi | Q8BL66 |
PeptideAtlasi | Q8BL66 |
PRIDEi | Q8BL66 |
ProteomicsDBi | 275903 |
Protocols and materials databases
Antibodypediai | 3127, 524 antibodies from 44 providers |
DNASUi | 216238 |
Genome annotation databases
Ensembli | ENSMUST00000053484; ENSMUSP00000061493; ENSMUSG00000036499 |
GeneIDi | 216238 |
KEGGi | mmu:216238 |
UCSCi | uc007gwu.1, mouse |
Organism-specific databases
CTDi | 8411 |
MGIi | MGI:2442192, Eea1 |
VEuPathDBi | HostDB:ENSMUSG00000036499 |
Phylogenomic databases
eggNOGi | ENOG502QWB5, Eukaryota |
GeneTreei | ENSGT00940000156910 |
HOGENOMi | CLU_256550_0_0_1 |
InParanoidi | Q8BL66 |
OMAi | FIAVYQH |
OrthoDBi | 753155at2759 |
PhylomeDBi | Q8BL66 |
TreeFami | TF329698 |
Enzyme and pathway databases
Reactomei | R-MMU-168138, Toll Like Receptor 9 (TLR9) Cascade |
Miscellaneous databases
BioGRID-ORCSi | 216238, 4 hits in 73 CRISPR screens |
ChiTaRSi | Eea1, mouse |
PROi | PR:Q8BL66 |
RNActi | Q8BL66, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000036499, Expressed in secondary oocyte and 307 other tissues |
Genevisiblei | Q8BL66, MM |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR013087, Znf_C2H2_type IPR000306, Znf_FYVE IPR017455, Znf_FYVE-rel IPR011011, Znf_FYVE_PHD IPR013083, Znf_RING/FYVE/PHD |
Pfami | View protein in Pfam PF01363, FYVE, 1 hit |
SMARTi | View protein in SMART SM00064, FYVE, 1 hit |
SUPFAMi | SSF57903, SSF57903, 1 hit |
PROSITEi | View protein in PROSITE PS50178, ZF_FYVE, 1 hit PS00028, ZINC_FINGER_C2H2_1, 1 hit PS50157, ZINC_FINGER_C2H2_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | EEA1_MOUSE | |
Accessioni | Q8BL66Primary (citable) accession number: Q8BL66 Secondary accession number(s): Q6DIC2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 22, 2003 |
Last sequence update: | September 27, 2005 | |
Last modified: | May 25, 2022 | |
This is version 156 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot