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Protein

Trinucleotide repeat-containing gene 6B protein

Gene

Tnrc6b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent translational repression and siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins As scaffoldng protein associates with argonaute proteins bound to partially complementary mRNAs and simultaneously can recruit CCR4-NOT and PAN deadenylase complexes (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processRNA-mediated gene silencing, Translation regulation

Enzyme and pathway databases

ReactomeiR-MMU-426496 Post-transcriptional silencing by small RNAs

Names & Taxonomyi

Protein namesi
Recommended name:
Trinucleotide repeat-containing gene 6B protein
Gene namesi
Name:Tnrc6b
Synonyms:Kiaa1093
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:2443730 Tnrc6b

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003739811 – 1810Trinucleotide repeat-containing gene 6B proteinAdd BLAST1810

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei913PhosphoserineBy similarity1
Modified residuei1409PhosphoserineCombined sources1
Modified residuei1426PhosphothreonineCombined sources1
Modified residuei1438PhosphoserineCombined sources1
Modified residuei1441PhosphothreonineCombined sources1
Modified residuei1793PhosphoserineBy similarity1
Modified residuei1809PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8BKI2
PaxDbiQ8BKI2
PeptideAtlasiQ8BKI2
PRIDEiQ8BKI2

PTM databases

iPTMnetiQ8BKI2
PhosphoSitePlusiQ8BKI2

Expressioni

Gene expression databases

BgeeiENSMUSG00000047888 Expressed in 265 organ(s), highest expression level in retina
GenevisibleiQ8BKI2 MM

Interactioni

Subunit structurei

Interacts with AGO1, AGO2, AGO3 and AGO4. Interacts with CNOT1; the interaction mediates the association with the CCR4-NOT complex. Interacts with PAN3; the interaction mediates the association with the PAN complex (By similarity).By similarity

Protein-protein interaction databases

BioGridi229484, 3 interactors
DIPiDIP-48568N
IntActiQ8BKI2, 3 interactors
STRINGi10090.ENSMUSP00000064336

Structurei

3D structure databases

ProteinModelPortaliQ8BKI2
SMRiQ8BKI2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1625 – 1697RRMAdd BLAST73

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni37 – 1028Interaction with argonaute proteinsBy similarityAdd BLAST992
Regioni1191 – 1700Silencing domain; interaction with CNOT1 and PAN3By similarityAdd BLAST510
Regioni1449 – 1467PABPC1-interacting motif-2 (PAM2)By similarityAdd BLAST19

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili33 – 75Sequence analysisAdd BLAST43

Sequence similaritiesi

Belongs to the GW182 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IET2 Eukaryota
ENOG410YDKH LUCA
GeneTreeiENSGT00410000025966
HOGENOMiHOG000049171
InParanoidiQ8BKI2
KOiK18412
OMAiDIEEMPR
OrthoDBiEOG091G00G6
PhylomeDBiQ8BKI2
TreeFamiTF329702

Family and domain databases

CDDicd12712 RRM_TNRC6B, 1 hit
Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR019486 Argonaute_hook_dom
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR032226 TNRC6_PABC-bd
IPR033500 TNRC6B
IPR034925 TNRC6B_RRM
PANTHERiPTHR13020:SF32 PTHR13020:SF32, 1 hit
PfamiView protein in Pfam
PF10427 Ago_hook, 1 hit
PF16608 TNRC6-PABC_bdg, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8BKI2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MQTNEGEVEE ESSSQVEQED FVMEGHGKTP PPGEESKQEK EQEREEQLME
60 70 80 90 100
DKKRKKEDKK KKEATQKVTE QKTKVPEVTK PSLSQPTAAS PIGSSPSPPV
110 120 130 140 150
NGGNNAKRVA VPNGQPPSAA RYMPREVPPR FRCQQDHKVL LKRGQPPPPS
160 170 180 190 200
CMLLGGGAGP PPCTAPGANP NNNAQVTGAL LQSESGTAPE STLGGAAASN
210 220 230 240 250
YANSTWGPGA SSNSGASPNP IHIWDKVIVD GSDMEEWPCI ASKDTESSSE
260 270 280 290 300
NTTDNNSASN PGSEKSSLPG STTSNKGKGS QCQAASSGNE CNLGVWKSDP
310 320 330 340 350
KAKSVQPPNS TSDSNNGLGN WRSTSGQDRI GPGSGFSNFN PNSNPSAWPA
360 370 380 390 400
LVQEGTSRKG ALETESSSSS AQVSTVGQAS REQQSKMENA GVNFVVSGRE
410 420 430 440 450
QAQIHNTDGP KNGNTNSLNL SSPNPMENKG MPFGMGLGNT SRSTDAPSQS
460 470 480 490 500
TGDRKTGSVG SWGAARGPSG TDTVSGQSNS GNNGNNGKDR EDSWKGASVP
510 520 530 540 550
KPTGSKSDSW DNNNRSTGGS WNFGPQDNND NKWGEGNKMT SGVSQGEWKQ
560 570 580 590 600
PTGSDELKIG EWSGPNQPNS STGAWDNQKG HPLPENQGNA QAPCWGRSSS
610 620 630 640 650
SAGSEVGGQS TGSNHKAGSS DSHNSGRRSY RPTHPDCQAV LQTLLSRTDL
660 670 680 690 700
DPRVLSNTGW GQTQIKQDTV WDIEEVPRPE GKSDKGTEGW ESAATQTKNS
710 720 730 740 750
GGWGDAPSQS NQMKSGWGEL SASTEWKDPK STGGWNDYKN NNSSNWGGGR
760 770 780 790 800
ADEKTPSSWN ESSCKDQGWG GGRQPNQGWT SGKNGWGEEV DQVKNNNWES
810 820 830 840 850
SANKPVSGWG EGGQNEIGTW GNGGNTNLAS KGGWEDCKRS PAWNETGRQP
860 870 880 890 900
NSWNKQHQQQ QQPPPPQPEA SGSWGGPPPP PQGNVRPSNS NWSSGPQPTT
910 920 930 940 950
PKDDEPSGWE EPSPQSISRK MDIDDGTSAW GDPNSYNYKN VNLWDKNSQG
960 970 980 990 1000
GPAPREPNLP TPMTGKSASV WSKSTPPAPD NGTSAWGEPN ESSPGWGEMD
1010 1020 1030 1040 1050
DAGASTTGWG NTPANAPNAM KPNSKSMQDG WGESDGPVTG ARHPSWEEED
1060 1070 1080 1090 1100
DGGVWNTAGS QGSTSSHNSA SWGQGGKKQM KCSLKGGNND SWMNPLAKQF
1110 1120 1130 1140 1150
SNMGLLSQTE DNPSSKMDLS VDKKFDVDKR TMNLGDFNDI MRKDRPGFRP
1160 1170 1180 1190 1200
PNSKDLGTTD SGPYFEKGGS HGLFGNSTAQ SRGLHTPVQP LSSSPGLRAQ
1210 1220 1230 1240 1250
VPPQFISPQV SASMLKQFPN SGLNPGLFNV GPQLSPQQIA MLSQLPQIPQ
1260 1270 1280 1290 1300
FQLACQLLLQ QQQQQQQLLQ NQRKISQAVR QQQEQQLARM VSALQQQQQQ
1310 1320 1330 1340 1350
QQQQQRQPSM KHSPSHPVGP KPHLDNMVPN ALNVGLPDLP TKGPIPGYGS
1360 1370 1380 1390 1400
GFSSGGMDYG MVGGKEAGTE SRFKQWTSMM EGLPSVATQE ATMHKNGAIV
1410 1420 1430 1440 1450
APGKTRGGSP YNQFDIIPGD TLGGHTGPAG DSWLPAKSPP TNKIGSKSSN
1460 1470 1480 1490 1500
ASWPPEFQPG VPWKGIQNID PESDPYVTPG SVLGGTTTSP IVDTDHQLLR
1510 1520 1530 1540 1550
DNTTGSNSSL NTSLPSPGAW PYSASDNSFT NVHSTSAKFP DYKSTWSPDP
1560 1570 1580 1590 1600
IGHNPTHLSN KMWKNHISSR NTTPLTRPPP GLTNPKPASP WSSTAPRSVR
1610 1620 1630 1640 1650
GWGTQDSRIA SASTWSDGGS VRPSYWLVLH NLTPQIDGST LRTICMQHGP
1660 1670 1680 1690 1700
LLTFHLNLTQ GTALIRYSTK QEAAKAQTAL HMCVLGNTTI LAEFATEDEV
1710 1720 1730 1740 1750
SRFLAQAQPP TPAATPSAPA TGWQSLETSQ NQADPVGPAL NLFGGSTGLG
1760 1770 1780 1790 1800
QWSSSAGGSS GADLAGTSLW GPPNYSSSLW GVPTVEDPHR MGSPAPLLPG
1810
DLLGGGSDSI
Length:1,810
Mass (Da):191,963
Last modified:May 5, 2009 - v2
Checksum:i70F0A3A88CE7C7C5
GO
Isoform 2 (identifier: Q8BKI2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-36: Missing.
     37-38: KQ → MR
     970-979: VWSKSTPPAP → GRYQLFSHRS
     980-1810: Missing.

Show »
Length:943
Mass (Da):99,891
Checksum:iB11D333CE3B24E15
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A2I3BRG1A0A2I3BRG1_MOUSE
Trinucleotide repeat-containing gen...
Tnrc6b
958Annotation score:
A0A2I3BPE5A0A2I3BPE5_MOUSE
Trinucleotide repeat-containing gen...
Tnrc6b
61Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti904D → Y in BAC34813 (PubMed:16141072).Curated1
Sequence conflicti1576T → P in BAC65722 (PubMed:12693553).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0372941 – 36Missing in isoform 2. 1 PublicationAdd BLAST36
Alternative sequenceiVSP_03729537 – 38KQ → MR in isoform 2. 1 Publication2
Alternative sequenceiVSP_037296970 – 979VWSKSTPPAP → GRYQLFSHRS in isoform 2. 1 Publication10
Alternative sequenceiVSP_037297980 – 1810Missing in isoform 2. 1 PublicationAdd BLAST831

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC125540 Genomic DNA No translation available.
AC126682 Genomic DNA No translation available.
AK051922 mRNA Translation: BAC34813.1
AK122440 mRNA Translation: BAC65722.1
CCDSiCCDS37146.1 [Q8BKI2-1]
RefSeqiNP_659061.2, NM_144812.2 [Q8BKI2-1]
UniGeneiMm.131328
Mm.412092

Genome annotation databases

EnsembliENSMUST00000067689; ENSMUSP00000064336; ENSMUSG00000047888 [Q8BKI2-1]
GeneIDi213988
KEGGimmu:213988
UCSCiuc007wvt.1 mouse [Q8BKI2-1]
uc007wvu.2 mouse [Q8BKI2-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC125540 Genomic DNA No translation available.
AC126682 Genomic DNA No translation available.
AK051922 mRNA Translation: BAC34813.1
AK122440 mRNA Translation: BAC65722.1
CCDSiCCDS37146.1 [Q8BKI2-1]
RefSeqiNP_659061.2, NM_144812.2 [Q8BKI2-1]
UniGeneiMm.131328
Mm.412092

3D structure databases

ProteinModelPortaliQ8BKI2
SMRiQ8BKI2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi229484, 3 interactors
DIPiDIP-48568N
IntActiQ8BKI2, 3 interactors
STRINGi10090.ENSMUSP00000064336

PTM databases

iPTMnetiQ8BKI2
PhosphoSitePlusiQ8BKI2

Proteomic databases

EPDiQ8BKI2
PaxDbiQ8BKI2
PeptideAtlasiQ8BKI2
PRIDEiQ8BKI2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000067689; ENSMUSP00000064336; ENSMUSG00000047888 [Q8BKI2-1]
GeneIDi213988
KEGGimmu:213988
UCSCiuc007wvt.1 mouse [Q8BKI2-1]
uc007wvu.2 mouse [Q8BKI2-2]

Organism-specific databases

CTDi23112
MGIiMGI:2443730 Tnrc6b
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410IET2 Eukaryota
ENOG410YDKH LUCA
GeneTreeiENSGT00410000025966
HOGENOMiHOG000049171
InParanoidiQ8BKI2
KOiK18412
OMAiDIEEMPR
OrthoDBiEOG091G00G6
PhylomeDBiQ8BKI2
TreeFamiTF329702

Enzyme and pathway databases

ReactomeiR-MMU-426496 Post-transcriptional silencing by small RNAs

Miscellaneous databases

ChiTaRSiTnrc6b mouse
PROiPR:Q8BKI2
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000047888 Expressed in 265 organ(s), highest expression level in retina
GenevisibleiQ8BKI2 MM

Family and domain databases

CDDicd12712 RRM_TNRC6B, 1 hit
Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR019486 Argonaute_hook_dom
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR032226 TNRC6_PABC-bd
IPR033500 TNRC6B
IPR034925 TNRC6B_RRM
PANTHERiPTHR13020:SF32 PTHR13020:SF32, 1 hit
PfamiView protein in Pfam
PF10427 Ago_hook, 1 hit
PF16608 TNRC6-PABC_bdg, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTNR6B_MOUSE
AccessioniPrimary (citable) accession number: Q8BKI2
Secondary accession number(s): Q80TK4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 5, 2009
Last modified: September 12, 2018
This is version 113 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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