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Protein

Target of rapamycin complex 2 subunit MAPKAP1

Gene

Mapkap1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Isoform 1 is involved in ciliogenesis, regulates cilia length through its interaction with CCDC28B independently of mTORC2 complex.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processStress response

Enzyme and pathway databases

ReactomeiR-MMU-1257604 PIP3 activates AKT signaling
R-MMU-389357 CD28 dependent PI3K/Akt signaling
R-MMU-5218920 VEGFR2 mediated vascular permeability
R-MMU-6804757 Regulation of TP53 Degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Target of rapamycin complex 2 subunit MAPKAP1
Short name:
TORC2 subunit MAPKAP1
Alternative name(s):
Mitogen-activated protein kinase 2-associated protein 1
Stress-activated map kinase-interacting protein 1
Short name:
SAPK-interacting protein 1
Gene namesi
Name:Mapkap1
Synonyms:Mip1, Sin1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2444554 Mapkap1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane, Nucleus

Pathology & Biotechi

Disruption phenotypei

Death during early embryonic stages.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00003280332 – 522Target of rapamycin complex 2 subunit MAPKAP1Add BLAST521

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei186PhosphoserineBy similarity1
Modified residuei510PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ8BKH7
PeptideAtlasiQ8BKH7
PRIDEiQ8BKH7

PTM databases

iPTMnetiQ8BKH7
PhosphoSitePlusiQ8BKH7

Expressioni

Tissue specificityi

Uniquitously expresseed, with highest levels in testis, kidney and liver. Present in renal tubule cells (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000038696 Expressed in 270 organ(s), highest expression level in liver
ExpressionAtlasiQ8BKH7 baseline and differential
GenevisibleiQ8BKH7 MM

Interactioni

Subunit structurei

All isoforms except isoform 4 can be incorporated into the mammalian target of rapamycin complex 2 (mTORC2) which contains MTOR, MLST8, PRR5, RICTOR, MAPKAP1 and DEPTOR. Contrary to mTORC1, mTORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Interacts with ATF2, MAP3K2 and MAPK8. Interacts with GTP-bound HRAS and KRAS. Interacts with IFNAR2 and SGK1. Isoform 2 interacts with NBN. Isoform 1 interacts with CCDC28B.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi230681, 3 interactors
DIPiDIP-57240N
IntActiQ8BKH7, 7 interactors
MINTiQ8BKH7
STRINGi10090.ENSMUSP00000108751

Structurei

3D structure databases

ProteinModelPortaliQ8BKH7
SMRiQ8BKH7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 267Interaction with NBNBy similarityAdd BLAST266
Regioni2 – 184Interaction with MAP3K2By similarityAdd BLAST183
Regioni468 – 522Interaction with ATF2By similarityAdd BLAST55

Sequence similaritiesi

Belongs to the SIN1 family.Curated

Phylogenomic databases

eggNOGiKOG3739 Eukaryota
ENOG410XR57 LUCA
GeneTreeiENSGT00390000000642
HOVERGENiHBG023148
InParanoidiQ8BKH7
KOiK20410
OMAiNAKFWPQ
OrthoDBiEOG091G0AVW
PhylomeDBiQ8BKH7
TreeFamiTF315174

Family and domain databases

Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR031567 CRIM_dom
IPR011993 PH-like_dom_sf
IPR032679 Sin1_N
IPR031313 Sin1_PH_dom
PfamiView protein in Pfam
PF16978 CRIM, 1 hit
PF05422 SIN1, 1 hit
PF16979 SIN1_PH, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8BKH7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAFLDNPTII LAHIRQSHVT SDDTGMCEMV LIDHDVDLEK THPPSVPGDS
60 70 80 90 100
GSEVQGSSGE TQGYIYAQSV DITSSWDFGI RRRSNTAQRL ERLRKERQNQ
110 120 130 140 150
IKCKNIQWKE RNSKQSAQEL KSLFEKKSLK EKPPSSGKQS ILSVRLEQCP
160 170 180 190 200
LQLNNPFNEY SKFDGKGHVG TTATKKIDVY LPLHSSQDRL LPMTVVTMAS
210 220 230 240 250
ARVQDLIGLI CWQYTSEGRE PKLNDNVSAY CLHIAEDDGE VDTDFPPLDS
260 270 280 290 300
NEPIHKFGFS TLALVEKYSS PGLTSKESLF VRINAAHGFS LIQVDNTKVT
310 320 330 340 350
MKEILLKAVK RRKGSQKISG PQYRLEKQSE PNIAVDLEST LESQNAWEFC
360 370 380 390 400
LVRENSSRAD GVFEEDSQID IATVQDMLSS HHYKSFKVSM IHRLRFTTDV
410 420 430 440 450
QLGISGDKVE IDPVTNQKAS TKFWIKQKPI SIDCDLLCAC DLAEEKSPSH
460 470 480 490 500
AVFKLTYLSS HDYKHLYFES DAATVSEIVL KVNYILESRA STARADYLAQ
510 520
KQRKLNRRTS FSFQKEKKSG QQ
Length:522
Mass (Da):59,009
Last modified:March 1, 2003 - v1
Checksum:i994C669D04065926
GO
Isoform 2 (identifier: Q8BKH7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     321-356: Missing.

Show »
Length:486
Mass (Da):54,802
Checksum:iF42F6C1899858727
GO
Isoform 3 (identifier: Q8BKH7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-192: Missing.

Show »
Length:330
Mass (Da):37,303
Checksum:iFA1ED1D41CF5CEA6
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0A6YW60A0A0A6YW60_MOUSE
Target of rapamycin complex 2 subun...
Mapkap1
171Annotation score:
A2AR15A2AR15_MOUSE
Target of rapamycin complex 2 subun...
Mapkap1
306Annotation score:
A0A0A6YVX6A0A0A6YVX6_MOUSE
Target of rapamycin complex 2 subun...
Mapkap1
20Annotation score:
A0A0A6YY51A0A0A6YY51_MOUSE
Target of rapamycin complex 2 subun...
Mapkap1
40Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti516E → K in AAH43296 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0332081 – 192Missing in isoform 3. CuratedAdd BLAST192
Alternative sequenceiVSP_033209321 – 356Missing in isoform 2. CuratedAdd BLAST36

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK027932 mRNA Translation: BAC25671.1
AK052045 mRNA Translation: BAC34838.1
AK132263 mRNA Translation: BAE21066.1
AL845262, AL808102 Genomic DNA Translation: CAM15450.1
AL845262, AL808102 Genomic DNA Translation: CAM15451.1
AL845262, AL808102 Genomic DNA Translation: CAM15454.1
AL808102, AL845262 Genomic DNA Translation: CAM17090.1
AL808102, AL845262 Genomic DNA Translation: CAM17091.1
AL808102, AL845262 Genomic DNA Translation: CAM17094.1
BC027377 mRNA Translation: AAH27377.1
BC031579 mRNA Translation: AAH31579.1
BC043296 mRNA Translation: AAH43296.1
BC090644 mRNA Translation: AAH90644.1
BC096618 mRNA Translation: AAH96618.1
CCDSiCCDS15948.1 [Q8BKH7-1]
CCDS71035.1 [Q8BKH7-3]
RefSeqiNP_001277554.1, NM_001290625.1 [Q8BKH7-1]
NP_001277555.1, NM_001290626.1 [Q8BKH7-3]
NP_796319.1, NM_177345.4 [Q8BKH7-1]
XP_006498038.1, XM_006497975.2 [Q8BKH7-1]
XP_006498041.1, XM_006497978.3 [Q8BKH7-2]
XP_006498045.1, XM_006497982.2
XP_006498046.1, XM_006497983.2 [Q8BKH7-3]
XP_011237384.1, XM_011239082.2
XP_011237385.1, XM_011239083.2 [Q8BKH7-1]
XP_011237386.1, XM_011239084.2 [Q8BKH7-1]
XP_011237387.1, XM_011239085.2
XP_017172932.1, XM_017317443.1
UniGeneiMm.270866
Mm.487284

Genome annotation databases

EnsembliENSMUST00000113123; ENSMUSP00000108748; ENSMUSG00000038696 [Q8BKH7-3]
ENSMUST00000113124; ENSMUSP00000108749; ENSMUSG00000038696 [Q8BKH7-2]
ENSMUST00000113126; ENSMUSP00000108751; ENSMUSG00000038696 [Q8BKH7-1]
ENSMUST00000147337; ENSMUSP00000116494; ENSMUSG00000038696 [Q8BKH7-1]
GeneIDi227743
KEGGimmu:227743
UCSCiuc008jil.2 mouse [Q8BKH7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK027932 mRNA Translation: BAC25671.1
AK052045 mRNA Translation: BAC34838.1
AK132263 mRNA Translation: BAE21066.1
AL845262, AL808102 Genomic DNA Translation: CAM15450.1
AL845262, AL808102 Genomic DNA Translation: CAM15451.1
AL845262, AL808102 Genomic DNA Translation: CAM15454.1
AL808102, AL845262 Genomic DNA Translation: CAM17090.1
AL808102, AL845262 Genomic DNA Translation: CAM17091.1
AL808102, AL845262 Genomic DNA Translation: CAM17094.1
BC027377 mRNA Translation: AAH27377.1
BC031579 mRNA Translation: AAH31579.1
BC043296 mRNA Translation: AAH43296.1
BC090644 mRNA Translation: AAH90644.1
BC096618 mRNA Translation: AAH96618.1
CCDSiCCDS15948.1 [Q8BKH7-1]
CCDS71035.1 [Q8BKH7-3]
RefSeqiNP_001277554.1, NM_001290625.1 [Q8BKH7-1]
NP_001277555.1, NM_001290626.1 [Q8BKH7-3]
NP_796319.1, NM_177345.4 [Q8BKH7-1]
XP_006498038.1, XM_006497975.2 [Q8BKH7-1]
XP_006498041.1, XM_006497978.3 [Q8BKH7-2]
XP_006498045.1, XM_006497982.2
XP_006498046.1, XM_006497983.2 [Q8BKH7-3]
XP_011237384.1, XM_011239082.2
XP_011237385.1, XM_011239083.2 [Q8BKH7-1]
XP_011237386.1, XM_011239084.2 [Q8BKH7-1]
XP_011237387.1, XM_011239085.2
XP_017172932.1, XM_017317443.1
UniGeneiMm.270866
Mm.487284

3D structure databases

ProteinModelPortaliQ8BKH7
SMRiQ8BKH7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230681, 3 interactors
DIPiDIP-57240N
IntActiQ8BKH7, 7 interactors
MINTiQ8BKH7
STRINGi10090.ENSMUSP00000108751

PTM databases

iPTMnetiQ8BKH7
PhosphoSitePlusiQ8BKH7

Proteomic databases

PaxDbiQ8BKH7
PeptideAtlasiQ8BKH7
PRIDEiQ8BKH7

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000113123; ENSMUSP00000108748; ENSMUSG00000038696 [Q8BKH7-3]
ENSMUST00000113124; ENSMUSP00000108749; ENSMUSG00000038696 [Q8BKH7-2]
ENSMUST00000113126; ENSMUSP00000108751; ENSMUSG00000038696 [Q8BKH7-1]
ENSMUST00000147337; ENSMUSP00000116494; ENSMUSG00000038696 [Q8BKH7-1]
GeneIDi227743
KEGGimmu:227743
UCSCiuc008jil.2 mouse [Q8BKH7-1]

Organism-specific databases

CTDi79109
MGIiMGI:2444554 Mapkap1

Phylogenomic databases

eggNOGiKOG3739 Eukaryota
ENOG410XR57 LUCA
GeneTreeiENSGT00390000000642
HOVERGENiHBG023148
InParanoidiQ8BKH7
KOiK20410
OMAiNAKFWPQ
OrthoDBiEOG091G0AVW
PhylomeDBiQ8BKH7
TreeFamiTF315174

Enzyme and pathway databases

ReactomeiR-MMU-1257604 PIP3 activates AKT signaling
R-MMU-389357 CD28 dependent PI3K/Akt signaling
R-MMU-5218920 VEGFR2 mediated vascular permeability
R-MMU-6804757 Regulation of TP53 Degradation

Miscellaneous databases

ChiTaRSiMapkap1 mouse
PROiPR:Q8BKH7
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038696 Expressed in 270 organ(s), highest expression level in liver
ExpressionAtlasiQ8BKH7 baseline and differential
GenevisibleiQ8BKH7 MM

Family and domain databases

Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR031567 CRIM_dom
IPR011993 PH-like_dom_sf
IPR032679 Sin1_N
IPR031313 Sin1_PH_dom
PfamiView protein in Pfam
PF16978 CRIM, 1 hit
PF05422 SIN1, 1 hit
PF16979 SIN1_PH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSIN1_MOUSE
AccessioniPrimary (citable) accession number: Q8BKH7
Secondary accession number(s): A2AN72
, A2AN74, A2AR13, A2AR16, Q80UY4, Q8BMV5, Q8R2N9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: March 1, 2003
Last modified: November 7, 2018
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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