UniProtKB - Q8BKF1 (RPOM_MOUSE)
DNA-directed RNA polymerase, mitochondrial
Polrmt
Functioni
Catalytic activityi
- EC:2.7.7.6PROSITE-ProRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 893 | By similarity | 1 | |
Active sitei | 962 | By similarity | 1 | |
Active sitei | 1121 | By similarity | 1 |
GO - Molecular functioni
- DNA-directed 5'-3' RNA polymerase activity Source: MGI
- mitochondrial promoter sequence-specific DNA binding Source: GO_Central
- sequence-specific DNA binding Source: UniProtKB
GO - Biological processi
- mitochondrial transcription Source: MGI
Keywordsi
Molecular function | Nucleotidyltransferase, Transferase |
Biological process | Transcription |
Enzyme and pathway databases
Reactomei | R-MMU-163282, Mitochondrial transcription initiation |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:PolrmtImported |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1915843, Polrmt |
Subcellular locationi
Mitochondrion
- Mitochondrion 1 Publication
Mitochondrion
- mitochondrial DNA-directed RNA polymerase complex Source: GO_Central
- mitochondrial matrix Source: MGI
- mitochondrial nucleoid Source: MGI
- mitochondrion Source: MGI
Other locations
- protein-containing complex Source: UniProtKB
Keywords - Cellular componenti
DNA-directed RNA polymerase, MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 41 | MitochondrionSequence analysisAdd BLAST | 41 | |
ChainiPRO_0000031069 | 42 – 1207 | DNA-directed RNA polymerase, mitochondrialAdd BLAST | 1166 |
Proteomic databases
EPDi | Q8BKF1 |
MaxQBi | Q8BKF1 |
PaxDbi | Q8BKF1 |
PRIDEi | Q8BKF1 |
PTM databases
iPTMneti | Q8BKF1 |
PhosphoSitePlusi | Q8BKF1 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000020329, Expressed in adult mammalian kidney and 289 other tissues |
Genevisiblei | Q8BKF1, MM |
Interactioni
Subunit structurei
Homodimer.
Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT. In this complex TFAM recruits POLRMT to the promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand (By similarity). Upon metabolic stress, forms a complex composed of FOXO3, SIRT3 and mitochondrial RNA polymerase POLRMT; the complex is recruited to mtDNA in a SIRT3-dependent manner (PubMed:23283301). Also forms a complex composed of FOXO3, SIRT3, TFAM and POLRMT.
Interacts with TFB1M and TFB2M, leading to the stimulation of transcription.
Interacts with TEFM.
Interacts with MTRES1 (By similarity).
By similarity1 PublicationProtein-protein interaction databases
BioGRIDi | 229707, 1 interactor |
STRINGi | 10090.ENSMUSP00000020580 |
Miscellaneous databases
RNActi | Q8BKF1, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 232 – 266 | PPR 1Add BLAST | 35 | |
Repeati | 267 – 302 | PPR 2Add BLAST | 36 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 773 – 1207 | Mediates interaction with TEFMBy similarityAdd BLAST | 435 |
Sequence similaritiesi
Keywords - Domaini
Repeat, Transit peptidePhylogenomic databases
eggNOGi | KOG1038, Eukaryota |
GeneTreei | ENSGT00390000008060 |
InParanoidi | Q8BKF1 |
OMAi | YAEEIMP |
OrthoDBi | 135329at2759 |
PhylomeDBi | Q8BKF1 |
TreeFami | TF105700 |
Family and domain databases
Gene3Di | 1.10.1320.10, 1 hit 1.25.40.10, 1 hit |
InterProi | View protein in InterPro IPR002092, DNA-dir_Rpol_phage-type IPR043502, DNA/RNA_pol_sf IPR002885, Pentatricopeptide_repeat IPR037159, RNA_POL_N_sf IPR029262, RPOL_N IPR011990, TPR-like_helical_dom_sf |
PANTHERi | PTHR10102, PTHR10102, 1 hit |
Pfami | View protein in Pfam PF00940, RNA_pol, 1 hit PF14700, RPOL_N, 1 hit |
SMARTi | View protein in SMART SM01311, RPOL_N, 1 hit |
SUPFAMi | SSF56672, SSF56672, 1 hit |
TIGRFAMsi | TIGR00756, PPR, 1 hit |
PROSITEi | View protein in PROSITE PS51375, PPR, 2 hits PS00900, RNA_POL_PHAGE_1, 1 hit PS00489, RNA_POL_PHAGE_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MSALRWTRSA AGLGRVLRSP GPHRPPSEEG TFGGFCSSRR SSAASPREQH
60 70 80 90 100
VLREWGHAEL LEVLEARVRQ LRAEGTPEMR VKKVQVDRPP QGHSSRWAQK
110 120 130 140 150
LEAEKRVKQR RQKEVDQQKQ ALTQEFWTLH KEPKIWNKKL AGYLQPSKKG
160 170 180 190 200
TPTNSEEKQL AQALQAALGR LSSREAEALA RKKAKAVEAQ ILVLQQKFLA
210 220 230 240 250
FFECCVCTGQ VPLAHHVLVT HHNNGDRQQV LTLHMYNTVM LGWARKGSFR
260 270 280 290 300
ELVYVFLMLK DAGLSPDLCS YAAALQCMGR RDQDVRTIQR CLKQMMEEGF
310 320 330 340 350
QPQLLFTDLV LEEEDRAALL RAVVKAEPAF RPPPQAPSPV NTSTLLKDIY
360 370 380 390 400
SKEGPVSYPK LHLPLDTLQD LFYQQLHVEL SSSVCVQSVE KAPVMSKEVI
410 420 430 440 450
EARKTLQALR EQWEVELLRV LRETKATMGR QAYEGQPTLY PFLCLLSEGE
460 470 480 490 500
FVSILMQVLK VLPAQGEPLI QLAHNLGLRV LNRHLVKQKQ VTNHVQKLGQ
510 520 530 540 550
RYSQYLQLLA SDTQVAPCLP REYWESLGPL EAPAQQPWSV PVLLQLGKQL
560 570 580 590 600
AELLVQAVQM PRSLAARQGA QRSIPVLYHV YSFRSYRQVG ILKPHPAFTH
610 620 630 640 650
LLETAAEPTL TFETTEVPML CPPLPWTSLH SGAYLLSSTK LMRATEGTTQ
660 670 680 690 700
HQRLLEQCPP AQLHGPLDAL TQLGNCAWRV NGHLLDLVLQ IFRDKGCMPL
710 720 730 740 750
GVPPPRSEAP RPARYQLPPG STPVHKSELR KELARCLKVA REMHSLRSEA
760 770 780 790 800
LYRLSLAQHL RHRVFWLPHN MDFRGRTYPC PPHFNHLGSD LARALLEFAE
810 820 830 840 850
GRPLGPRGLD WLKIHLINLT GLKKGDSLRM RLAFADEVME EILDSADNPL
860 870 880 890 900
TGRKWWMEAD EPWQTLACCM EVAHAVRSPD PAAYISHLPV HQDGSCNGLQ
910 920 930 940 950
HYAALGRDSV GAASVNLTPS DLPQDVYREV ATQVEEFRQQ DAKEGLRVAQ
960 970 980 990 1000
VLEGFISRKV VKQTVMTVVY GVTRYGGRLQ IEKRLRELSD FPQEFVWEAS
1010 1020 1030 1040 1050
HYLVRQVFKS LQEMFTSTRA IQHWLTESAN LISHAGWPVE WVTPLGIPII
1060 1070 1080 1090 1100
QPYHRESKVQ VKGGLQSITL TSSVDESQKP NTLKQKNGFP PNFIHSLDSS
1110 1120 1130 1140 1150
HMMLTALHCY RKGLIFVSVH DCFWTHAADI PTMNEVCREQ FVRLHSQPIL
1160 1170 1180 1190 1200
EDLAKFLKKR FCSVSSIKSL KSSERALVTK LQETLQSLPK TGTFDLGQVI
RSTYFFS
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PWD9 | E9PWD9_MOUSE | DNA-directed RNA polymerase | Polrmt | 1,133 | Annotation score: | ||
E0CY15 | E0CY15_MOUSE | DNA-directed RNA polymerase | Polrmt | 903 | Annotation score: | ||
F6TLB0 | F6TLB0_MOUSE | DNA-directed RNA polymerase | Polrmt | 393 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 23 | H → Q in BAC39500 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK053356 mRNA Translation: BAC35360.1 AK085666 mRNA Translation: BAC39500.1 |
CCDSi | CCDS23987.1 |
RefSeqi | NP_766139.2, NM_172551.3 |
Genome annotation databases
Ensembli | ENSMUST00000020580; ENSMUSP00000020580; ENSMUSG00000020329 |
GeneIDi | 216151 |
KEGGi | mmu:216151 |
UCSCi | uc007fzo.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK053356 mRNA Translation: BAC35360.1 AK085666 mRNA Translation: BAC39500.1 |
CCDSi | CCDS23987.1 |
RefSeqi | NP_766139.2, NM_172551.3 |
3D structure databases
SMRi | Q8BKF1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 229707, 1 interactor |
STRINGi | 10090.ENSMUSP00000020580 |
PTM databases
iPTMneti | Q8BKF1 |
PhosphoSitePlusi | Q8BKF1 |
Proteomic databases
EPDi | Q8BKF1 |
MaxQBi | Q8BKF1 |
PaxDbi | Q8BKF1 |
PRIDEi | Q8BKF1 |
Protocols and materials databases
Antibodypediai | 1270, 99 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000020580; ENSMUSP00000020580; ENSMUSG00000020329 |
GeneIDi | 216151 |
KEGGi | mmu:216151 |
UCSCi | uc007fzo.2, mouse |
Organism-specific databases
CTDi | 5442 |
MGIi | MGI:1915843, Polrmt |
Phylogenomic databases
eggNOGi | KOG1038, Eukaryota |
GeneTreei | ENSGT00390000008060 |
InParanoidi | Q8BKF1 |
OMAi | YAEEIMP |
OrthoDBi | 135329at2759 |
PhylomeDBi | Q8BKF1 |
TreeFami | TF105700 |
Enzyme and pathway databases
Reactomei | R-MMU-163282, Mitochondrial transcription initiation |
Miscellaneous databases
BioGRID-ORCSi | 216151, 7 hits in 19 CRISPR screens |
ChiTaRSi | Polrmt, mouse |
PROi | PR:Q8BKF1 |
RNActi | Q8BKF1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000020329, Expressed in adult mammalian kidney and 289 other tissues |
Genevisiblei | Q8BKF1, MM |
Family and domain databases
Gene3Di | 1.10.1320.10, 1 hit 1.25.40.10, 1 hit |
InterProi | View protein in InterPro IPR002092, DNA-dir_Rpol_phage-type IPR043502, DNA/RNA_pol_sf IPR002885, Pentatricopeptide_repeat IPR037159, RNA_POL_N_sf IPR029262, RPOL_N IPR011990, TPR-like_helical_dom_sf |
PANTHERi | PTHR10102, PTHR10102, 1 hit |
Pfami | View protein in Pfam PF00940, RNA_pol, 1 hit PF14700, RPOL_N, 1 hit |
SMARTi | View protein in SMART SM01311, RPOL_N, 1 hit |
SUPFAMi | SSF56672, SSF56672, 1 hit |
TIGRFAMsi | TIGR00756, PPR, 1 hit |
PROSITEi | View protein in PROSITE PS51375, PPR, 2 hits PS00900, RNA_POL_PHAGE_1, 1 hit PS00489, RNA_POL_PHAGE_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RPOM_MOUSE | |
Accessioni | Q8BKF1Primary (citable) accession number: Q8BKF1 Secondary accession number(s): Q8BJE0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2004 |
Last sequence update: | March 1, 2003 | |
Last modified: | December 2, 2020 | |
This is version 141 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families