UniProtKB - Q8BK75 (ELP6_MOUSE)
Elongator complex protein 6
Elp6
Functioni
Component of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine) (By similarity).
The elongator complex catalyzes formation of carboxymethyluridine in the wobble base at position 34 in tRNAs (By similarity).
Involved in cell migration (PubMed:22854966).
By similarity1 PublicationCaution
: 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis Pathwayi
This protein is involved in the pathway 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis, which is part of tRNA modification.By similarityView all proteins of this organism that are known to be involved in the pathway 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis and in tRNA modification.
GO - Biological processi
- positive regulation of cell migration Source: UniProtKB
- tRNA wobble uridine modification Source: InterPro
Keywordsi
Biological process | tRNA processing |
Enzyme and pathway databases
UniPathwayi | UPA00988 |
Names & Taxonomyi
Protein namesi | Recommended name: Elongator complex protein 6Alternative name(s): Protein TMEM103 |
Gene namesi | Name:Elp6 Synonyms:Tmem103 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1919349, Elp6 |
VEuPathDBi | HostDB:ENSMUSG00000054836 |
Subcellular locationi
Cytosol
- cytosol Source: MGI
Nucleus
- nucleus Source: MGI
Other locations
- elongator holoenzyme complex Source: UniProtKB
Pathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 126 | L → Q in wobbly mutant; destabilization of the elongator complex and reduced tRNA wobble base modification leading to protein misfolding and aggregation in Purkinje neurons (PN), PN degeneration, microgliosis triggered by the NLPR3 inflammasome and an ataxia-like phenotype. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000274361 | 1 – 266 | Elongator complex protein 6Add BLAST | 266 |
Proteomic databases
EPDi | Q8BK75 |
MaxQBi | Q8BK75 |
PaxDbi | Q8BK75 |
PeptideAtlasi | Q8BK75 |
PRIDEi | Q8BK75 |
ProteomicsDBi | 277788 [Q8BK75-1] 277789 [Q8BK75-2] |
PTM databases
PhosphoSitePlusi | Q8BK75 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000054836, Expressed in dentate gyrus of hippocampal formation granule cell and 252 other tissues |
ExpressionAtlasi | Q8BK75, baseline and differential |
Genevisiblei | Q8BK75, MM |
Interactioni
Subunit structurei
Component of the elongator complex which consists of ELP1, ELP2, ELP3, ELP4, ELP5 and ELP6.
By similarityProtein-protein interaction databases
BioGRIDi | 215321, 2 interactors |
STRINGi | 10090.ENSMUSP00000069017 |
Miscellaneous databases
RNActi | Q8BK75, protein |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG4723, Eukaryota |
GeneTreei | ENSGT00390000011734 |
InParanoidi | Q8BK75 |
OMAi | MFTELNS |
OrthoDBi | 1272502at2759 |
PhylomeDBi | Q8BK75 |
TreeFami | TF331346 |
Family and domain databases
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR018627, ELP6 IPR027417, P-loop_NTPase |
PANTHERi | PTHR16184, PTHR16184, 1 hit |
Pfami | View protein in Pfam PF09807, ELP6, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MFPELNNLLS TTPDKTEQGT LTLLCDAKTD GSFLVHHFLS FYLKANCKVC
60 70 80 90 100
FVALVQSFSH YNIVGQKLGV SLTAARDRGQ LVFLEGLKSS VEVLFHSQDE
110 120 130 140 150
PHPLQFLREA GTGNLQSLYT FIQDTLKPAD SEESPWKYPV LLVDNLSVLL
160 170 180 190 200
SLGVGAVAVL DFMQYCRATV CCELKGNVVA LVHDTEGATD EGNDTLLNGL
210 220 230 240 250
SHQSHLILRA EGLATGFCKD VHGQLSILWR RPSRSTAQRA QSLTYQYKIQ
260
DKNVSFFAKG MSPAVL
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2JGK0 | A0A0G2JGK0_MOUSE | Elongator complex protein 6 | Elp6 | 110 | Annotation score: | ||
A0A0G2JGM4 | A0A0G2JGM4_MOUSE | Elongator complex protein 6 | Elp6 | 166 | Annotation score: | ||
A0A0H2UH13 | A0A0H2UH13_MOUSE | Elongator complex protein 6 | Elp6 | 263 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_022723 | 1 – 18 | MFPEL…DKTEQ → MAGEVTQALGGA in isoform 2. 1 PublicationAdd BLAST | 18 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK011270 mRNA Translation: BAB27508.1 AK075986 mRNA Translation: BAC36098.1 BC048732 mRNA Translation: AAH48732.1 |
CCDSi | CCDS52937.1 [Q8BK75-1] |
RefSeqi | NP_001074850.1, NM_001081381.1 [Q8BK75-1] |
Genome annotation databases
Ensembli | ENSMUST00000199592; ENSMUSP00000142823; ENSMUSG00000054836 [Q8BK75-1] |
GeneIDi | 72341 |
KEGGi | mmu:72341 |
UCSCi | uc009rtv.1, mouse [Q8BK75-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK011270 mRNA Translation: BAB27508.1 AK075986 mRNA Translation: BAC36098.1 BC048732 mRNA Translation: AAH48732.1 |
CCDSi | CCDS52937.1 [Q8BK75-1] |
RefSeqi | NP_001074850.1, NM_001081381.1 [Q8BK75-1] |
3D structure databases
AlphaFoldDBi | Q8BK75 |
SMRi | Q8BK75 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 215321, 2 interactors |
STRINGi | 10090.ENSMUSP00000069017 |
PTM databases
PhosphoSitePlusi | Q8BK75 |
Proteomic databases
EPDi | Q8BK75 |
MaxQBi | Q8BK75 |
PaxDbi | Q8BK75 |
PeptideAtlasi | Q8BK75 |
PRIDEi | Q8BK75 |
ProteomicsDBi | 277788 [Q8BK75-1] 277789 [Q8BK75-2] |
Protocols and materials databases
Antibodypediai | 48948, 94 antibodies from 14 providers |
Genome annotation databases
Ensembli | ENSMUST00000199592; ENSMUSP00000142823; ENSMUSG00000054836 [Q8BK75-1] |
GeneIDi | 72341 |
KEGGi | mmu:72341 |
UCSCi | uc009rtv.1, mouse [Q8BK75-1] |
Organism-specific databases
CTDi | 54859 |
MGIi | MGI:1919349, Elp6 |
VEuPathDBi | HostDB:ENSMUSG00000054836 |
Phylogenomic databases
eggNOGi | KOG4723, Eukaryota |
GeneTreei | ENSGT00390000011734 |
InParanoidi | Q8BK75 |
OMAi | MFTELNS |
OrthoDBi | 1272502at2759 |
PhylomeDBi | Q8BK75 |
TreeFami | TF331346 |
Enzyme and pathway databases
UniPathwayi | UPA00988 |
Miscellaneous databases
BioGRID-ORCSi | 72341, 20 hits in 72 CRISPR screens |
PROi | PR:Q8BK75 |
RNActi | Q8BK75, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000054836, Expressed in dentate gyrus of hippocampal formation granule cell and 252 other tissues |
ExpressionAtlasi | Q8BK75, baseline and differential |
Genevisiblei | Q8BK75, MM |
Family and domain databases
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR018627, ELP6 IPR027417, P-loop_NTPase |
PANTHERi | PTHR16184, PTHR16184, 1 hit |
Pfami | View protein in Pfam PF09807, ELP6, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ELP6_MOUSE | |
Accessioni | Q8BK75Primary (citable) accession number: Q8BK75 Secondary accession number(s): Q9D0M9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 6, 2007 |
Last sequence update: | March 1, 2003 | |
Last modified: | May 25, 2022 | |
This is version 121 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families