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Protein

Casein kinase I isoform alpha

Gene

Csnk1a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis. May play a role in keratin cytoskeleton disassembly and thereby, it may regulate epithelial cell migration (By similarity).By similarity1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei46ATPPROSITE-ProRule annotation1
Active sitei136Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi23 – 31ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processCell cycle, Cell division, Mitosis, Wnt signaling pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-195253 Degradation of beta-catenin by the destruction complex
R-MMU-196299 Beta-catenin phosphorylation cascade
R-MMU-4641262 Disassembly of the destruction complex and recruitment of AXIN to the membrane
R-MMU-5610785 GLI3 is processed to GLI3R by the proteasome
R-MMU-5635838 Activation of SMO

Names & Taxonomyi

Protein namesi
Recommended name:
Casein kinase I isoform alpha (EC:2.7.11.1)
Short name:
CKI-alpha
Alternative name(s):
CK1
Gene namesi
Name:Csnk1a1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1934950 Csnk1a1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Nucleus

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5148

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001928232 – 337Casein kinase I isoform alphaAdd BLAST336

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei4PhosphoserineBy similarity1
Modified residuei8N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ8BK63
PRIDEiQ8BK63

PTM databases

iPTMnetiQ8BK63
PhosphoSitePlusiQ8BK63

Expressioni

Gene expression databases

BgeeiENSMUSG00000024576 Expressed in 308 organ(s), highest expression level in pineal body
CleanExiMM_CSNK1A1
ExpressionAtlasiQ8BK63 baseline and differential
GenevisibleiQ8BK63 MM

Interactioni

Subunit structurei

Monomer. Interacts with the Axin complex. Interacts with TUT1, leading to TUT1 phosphorylation. Interacts with FAM83H; recruits CSNK1A1 to keratin filaments.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi220237, 7 interactors
ComplexPortaliCPX-103 Beta-catenin destruction core complex, variant 1
CPX-448 Beta-catenin destruction core complex, variant 2
CPX-449 Beta-catenin destruction core complex, variant 3
CPX-452 Beta-catenin destruction core complex, variant 4
CPX-453 Beta-catenin destruction core complex, variant 5
CPX-456 Beta-catenin destruction core complex, variant 6
CPX-457 Beta-catenin destruction core complex, variant 7
CPX-458 Beta-catenin destruction core complex, variant 8
DIPiDIP-32408N
IntActiQ8BK63, 8 interactors
STRINGi10090.ENSMUSP00000128871

Structurei

3D structure databases

ProteinModelPortaliQ8BK63
SMRiQ8BK63
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini17 – 285Protein kinasePROSITE-ProRule annotationAdd BLAST269

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1164 Eukaryota
ENOG410XPGP LUCA
GeneTreeiENSGT00920000148982
HOGENOMiHOG000182055
HOVERGENiHBG000176
InParanoidiQ8BK63
TreeFamiTF354246

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8BK63-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MASSSGSKAE FIVGGKYKLV RKIGSGSFGD IYLAINITNG EEVAVKLESQ
60 70 80 90 100
KARHPQLLYE SKLYKILQGG VGIPHIRWYG QEKDYNVLVM DLLGPSLEDL
110 120 130 140 150
FNFCSRRFTM KTVLMLADQM ISRIEYVHTK NFIHRDIKPD NFLMGIGRHC
160 170 180 190 200
NKLFLIDFGL AKKYRDNRTR QHIPYREDKN LTGTARYASI NAHLGIEQSR
210 220 230 240 250
RDDMESLGYV LMYFNRTSLP WQGLKAATKK QKYEKISEKK MSTPVEVLCK
260 270 280 290 300
GFPAEFAMYL NYCRGLRFEE APDYMYLRQL FRILFRTLNH QYDYTFDWTM
310 320 330
LKQKAAQQAA SSSGQGQQAQ TPTGKQTDKT KSNMKGF
Length:337
Mass (Da):38,915
Last modified:May 10, 2004 - v2
Checksum:i1B2085AE33CAFAC2
GO
Isoform 2 (identifier: Q8BK63-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     325-337: KQTDKTKSNMKGF → F

Show »
Length:325
Mass (Da):37,567
Checksum:iB84DC84BDDC17854
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9Q2U6E9Q2U6_MOUSE
Casein kinase I isoform alpha
Csnk1a1
353Annotation score:
E9Q3W1E9Q3W1_MOUSE
Casein kinase I isoform alpha
Csnk1a1
365Annotation score:
E9PWB2E9PWB2_MOUSE
Casein kinase I isoform alpha
Csnk1a1
374Annotation score:
H7BXB1H7BXB1_MOUSE
Casein kinase I isoform alpha
Csnk1a1
334Annotation score:
E9Q4G7E9Q4G7_MOUSE
Casein kinase I isoform alpha
Csnk1a1
336Annotation score:
F6YBC9F6YBC9_MOUSE
Casein kinase I isoform alpha
Csnk1a1
159Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti126Y → N in AAH48081 (PubMed:15489334).Curated1
Sequence conflicti176R → K in BAC36161 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010252325 – 337KQTDK…NMKGF → F in isoform 2. 3 PublicationsAdd BLAST13

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK076070 mRNA Translation: BAC36161.1
AK078398 mRNA Translation: BAC37255.1
AK146873 mRNA Translation: BAE27496.1
BC002171 mRNA Translation: AAH02171.1
BC048081 mRNA Translation: AAH48081.1
X90945 mRNA Translation: CAA62440.1
CCDSiCCDS37838.1 [Q8BK63-2]
RefSeqiXP_006526451.1, XM_006526388.2
UniGeneiMm.26908
Mm.487032

Genome annotation databases

EnsembliENSMUST00000165123; ENSMUSP00000128871; ENSMUSG00000024576 [Q8BK63-2]
ENSMUST00000165721; ENSMUSP00000132083; ENSMUSG00000024576 [Q8BK63-1]
GeneIDi93687
UCSCiuc008fcc.1 mouse [Q8BK63-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK076070 mRNA Translation: BAC36161.1
AK078398 mRNA Translation: BAC37255.1
AK146873 mRNA Translation: BAE27496.1
BC002171 mRNA Translation: AAH02171.1
BC048081 mRNA Translation: AAH48081.1
X90945 mRNA Translation: CAA62440.1
CCDSiCCDS37838.1 [Q8BK63-2]
RefSeqiXP_006526451.1, XM_006526388.2
UniGeneiMm.26908
Mm.487032

3D structure databases

ProteinModelPortaliQ8BK63
SMRiQ8BK63
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi220237, 7 interactors
ComplexPortaliCPX-103 Beta-catenin destruction core complex, variant 1
CPX-448 Beta-catenin destruction core complex, variant 2
CPX-449 Beta-catenin destruction core complex, variant 3
CPX-452 Beta-catenin destruction core complex, variant 4
CPX-453 Beta-catenin destruction core complex, variant 5
CPX-456 Beta-catenin destruction core complex, variant 6
CPX-457 Beta-catenin destruction core complex, variant 7
CPX-458 Beta-catenin destruction core complex, variant 8
DIPiDIP-32408N
IntActiQ8BK63, 8 interactors
STRINGi10090.ENSMUSP00000128871

Chemistry databases

ChEMBLiCHEMBL5148

PTM databases

iPTMnetiQ8BK63
PhosphoSitePlusiQ8BK63

Proteomic databases

PaxDbiQ8BK63
PRIDEiQ8BK63

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000165123; ENSMUSP00000128871; ENSMUSG00000024576 [Q8BK63-2]
ENSMUST00000165721; ENSMUSP00000132083; ENSMUSG00000024576 [Q8BK63-1]
GeneIDi93687
UCSCiuc008fcc.1 mouse [Q8BK63-1]

Organism-specific databases

CTDi1452
MGIiMGI:1934950 Csnk1a1

Phylogenomic databases

eggNOGiKOG1164 Eukaryota
ENOG410XPGP LUCA
GeneTreeiENSGT00920000148982
HOGENOMiHOG000182055
HOVERGENiHBG000176
InParanoidiQ8BK63
TreeFamiTF354246

Enzyme and pathway databases

ReactomeiR-MMU-195253 Degradation of beta-catenin by the destruction complex
R-MMU-196299 Beta-catenin phosphorylation cascade
R-MMU-4641262 Disassembly of the destruction complex and recruitment of AXIN to the membrane
R-MMU-5610785 GLI3 is processed to GLI3R by the proteasome
R-MMU-5635838 Activation of SMO

Miscellaneous databases

ChiTaRSiCsnk1a1 mouse
PROiPR:Q8BK63
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024576 Expressed in 308 organ(s), highest expression level in pineal body
CleanExiMM_CSNK1A1
ExpressionAtlasiQ8BK63 baseline and differential
GenevisibleiQ8BK63 MM

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiKC1A_MOUSE
AccessioniPrimary (citable) accession number: Q8BK63
Secondary accession number(s): Q3UIL0
, Q64506, Q80XI2, Q99LY3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: September 12, 2018
This is version 154 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  2. SIMILARITY comments
    Index of protein domains and families
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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