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Protein

Ribosome biogenesis protein NOP53

Gene

Nop53

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Nucleolar protein which is involved in the integration of the 5S RNP into the ribosomal large subunit during ribosome biogenesis. In ribosome biogenesis, may also play a role in rRNA transcription (By similarity). Also functions as a nucleolar sensor that regulates the activation of p53/TP53 in response to ribosome biogenesis perturbation, DNA damage and other stress conditions. DNA damage or perturbation of ribosome biogenesis disrupt the interaction between NOP53 and RPL11 allowing RPL11 transport to the nucleoplasm where it can inhibit MDM2 and allow p53/TP53 activation (PubMed:21804542). It may also positively regulate the function of p53/TP53 in cell cycle arrest and apoptosis through direct interaction, preventing its MDM2-dependent ubiquitin-mediated proteasomal degradation. Originally identified as a tumor suppressor, it may also play a role in cell proliferation and apoptosis by positively regulating the stability of PTEN, thereby antagonizing the PI3K-AKT/PKB signaling pathway. May also inhibit cell proliferation and increase apoptosis through its interaction with NF2. May negatively regulate NPM1 by regulating its nucleoplasmic localization, oligomerization and ubiquitin-mediated proteasomal degradation. Thereby, may prevent NPM1 interaction with MYC and negatively regulate transcription mediated by the MYC-NPM1 complex. May also regulate cellular aerobic respiration. In the cellular response to viral infection, may play a role in the attenuation of interferon-beta through the inhibition of DDX58/RIG-1 (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processRibosome biogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosome biogenesis protein NOP53Curated
Alternative name(s):
Glioma tumor suppressor candidate region gene 2 proteinImported
PreS1-binding proteinImported
Gene namesi
Name:Nop53Imported
Synonyms:Gltscr2Imported, Pict11 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2154441 Nop53

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Knockout embryos are not able to form viable blastocysts. Transgenic mice lacking the expression of Nop53 in the thymus display a dramatic reduction of the number of thymic cells and of the size of the thymus.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00004409682 – 484Ribosome biogenesis protein NOP53Add BLAST483

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei29PhosphoserineBy similarity1
Modified residuei305PhosphoserineCombined sources1

Post-translational modificationi

Ubiquitin-mediated proteasomal degradation is regulated by c-JUN. It is associated with relocalization to the nucleoplasm and decreased homooligomerization.By similarity
Phosphorylated upon DNA damage probably by ATM and DNA-PK; may regulate NOP53 degradation.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ8BK35
PaxDbiQ8BK35
PeptideAtlasiQ8BK35
PRIDEiQ8BK35

PTM databases

iPTMnetiQ8BK35
PhosphoSitePlusiQ8BK35

Expressioni

Gene expression databases

BgeeiENSMUSG00000041560 Expressed in 264 organ(s), highest expression level in blood

Interactioni

Subunit structurei

Homooligomer. Interacts with PTEN; regulates PTEN phosphorylation and increases its stability (By similarity). Interacts with RPL11; retains RPL11 into the nucleolus (PubMed:21804542). Interacts with CDKN2A/isoform tumor suppressor ARF; the interaction is direct and promotes ARF nucleoplasmic relocalization and ubiquitin-mediated proteasomal degradation. Interacts with NPM1; the interaction is direct and competitive with MYC. Interacts with NF2 (via FERM domain); the interaction is direct. Interacts with p53/TP53 (via the oligomerization region); the interaction is direct and may prevent the MDM2-mediated proteasomal degradation of p53/TP53. Interacts with DDX58; may regulate DDX58 through USP15-mediated 'Lys-63'-linked deubiquitination. Interacts with UBTF (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ8BK35, 2 interactors
MINTiQ8BK35
STRINGi10090.ENSMUSP00000043981

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni148 – 437Mediates interaction with CDKN2A/isoform tumor suppressor ARFBy similarityAdd BLAST290
Regioni348 – 392Mediates interaction with human herpesvirus 8 protein ORF16By similarityAdd BLAST45
Regioni353 – 401Nucleolar localization signalBy similarityAdd BLAST49
Regioni402 – 484Nucleolar localization signalBy similarityAdd BLAST83

Sequence similaritiesi

Belongs to the NOP53 family.Curated

Phylogenomic databases

eggNOGiKOG2823 Eukaryota
ENOG4111GSN LUCA
GeneTreeiENSGT00390000017267
HOGENOMiHOG000070127
HOVERGENiHBG051849
InParanoidiQ8BK35
KOiK14840
OMAiKSAWRKT
OrthoDBiEOG091G0BKI
PhylomeDBiQ8BK35
TreeFamiTF313004

Family and domain databases

InterProiView protein in InterPro
IPR011687 Nop53/GLTSCR2
PANTHERiPTHR14211 PTHR14211, 1 hit
PfamiView protein in Pfam
PF07767 Nop53, 1 hit
PIRSFiPIRSF017302 Gltscr2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8BK35-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAAGGNRDGE KRGSRSQADS GFLGLRPTSV DPALRRRRRG PRNKKRGWRR
60 70 80 90 100
LAEEPLGLEV DQFLEDVRLQ ERTTGGLLAE APNEKLFFVD TGFKRKEPRK
110 120 130 140 150
KRTLVQKKSQ RLQKPLRVDL ALENHSKIPA PKDILAHQVP NAKKLRRKEE
160 170 180 190 200
LWEKLAKQGE LPRDVRKAQA RLLSPPTPKA KPGPQDIIER PFYDLWNPDN
210 220 230 240 250
PLDTPLIGQD AFFLEQTKKK GVRRPQRLHI KPSQVPAVEV IPAGASYNPT
260 270 280 290 300
FEDHQALLRE AHEVELQREK EAEKLERQLA LPTSEQAATQ ESVFREMCEG
310 320 330 340 350
LLEESDGEDE HEAGRAGQPE AGDGTTEISP TGAAGPEKRM EKKTEQQRRR
360 370 380 390 400
EKAARKLRVQ QAALRAARLQ HQELFRLRGI KAQVARRLAE LARRREQRRI
410 420 430 440 450
RRLAEADKPR RLGRLKYQAP DIDVQLSSEL SGSLRTLKPE GHILRDRFKS
460 470 480
FQKRNMIEPR ERAKFKRKYK VKLVEKRAYR EIQL
Length:484
Mass (Da):55,793
Last modified:March 1, 2003 - v1
Checksum:iEB67949BCBE92D44
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti317G → A in AAH17637 (PubMed:15489334).Curated1
Sequence conflicti385A → V in AAH17637 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY535001 mRNA Translation: AAS46029.1
AK077341 mRNA Translation: BAC36760.1
AK146916 mRNA Translation: BAE27528.1
AK151836 mRNA Translation: BAE30729.1
AK160070 mRNA Translation: BAE35605.1
AK169290 mRNA Translation: BAE41047.1
AC148990 Genomic DNA No translation available.
CH466654 Genomic DNA Translation: EDL42118.1
BC017637 mRNA Translation: AAH17637.1
CCDSiCCDS39779.1
RefSeqiNP_598592.2, NM_133831.3
UniGeneiMm.277634

Genome annotation databases

EnsembliENSMUST00000044158; ENSMUSP00000043981; ENSMUSG00000041560
GeneIDi68077
KEGGimmu:68077
UCSCiuc009fgr.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY535001 mRNA Translation: AAS46029.1
AK077341 mRNA Translation: BAC36760.1
AK146916 mRNA Translation: BAE27528.1
AK151836 mRNA Translation: BAE30729.1
AK160070 mRNA Translation: BAE35605.1
AK169290 mRNA Translation: BAE41047.1
AC148990 Genomic DNA No translation available.
CH466654 Genomic DNA Translation: EDL42118.1
BC017637 mRNA Translation: AAH17637.1
CCDSiCCDS39779.1
RefSeqiNP_598592.2, NM_133831.3
UniGeneiMm.277634

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BK35, 2 interactors
MINTiQ8BK35
STRINGi10090.ENSMUSP00000043981

PTM databases

iPTMnetiQ8BK35
PhosphoSitePlusiQ8BK35

Proteomic databases

MaxQBiQ8BK35
PaxDbiQ8BK35
PeptideAtlasiQ8BK35
PRIDEiQ8BK35

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000044158; ENSMUSP00000043981; ENSMUSG00000041560
GeneIDi68077
KEGGimmu:68077
UCSCiuc009fgr.2 mouse

Organism-specific databases

CTDi29997
MGIiMGI:2154441 Nop53

Phylogenomic databases

eggNOGiKOG2823 Eukaryota
ENOG4111GSN LUCA
GeneTreeiENSGT00390000017267
HOGENOMiHOG000070127
HOVERGENiHBG051849
InParanoidiQ8BK35
KOiK14840
OMAiKSAWRKT
OrthoDBiEOG091G0BKI
PhylomeDBiQ8BK35
TreeFamiTF313004

Miscellaneous databases

PROiPR:Q8BK35
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000041560 Expressed in 264 organ(s), highest expression level in blood

Family and domain databases

InterProiView protein in InterPro
IPR011687 Nop53/GLTSCR2
PANTHERiPTHR14211 PTHR14211, 1 hit
PfamiView protein in Pfam
PF07767 Nop53, 1 hit
PIRSFiPIRSF017302 Gltscr2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNOP53_MOUSE
AccessioniPrimary (citable) accession number: Q8BK35
Secondary accession number(s): Q8VD18
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2017
Last sequence update: March 1, 2003
Last modified: November 7, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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