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Protein

F-box only protein 9

Gene

Fbxo9

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of TTI1 and TELO2 in a CK2-dependent manner, thereby directly regulating mTOR signaling. SCF(FBXO9) recognizes and binds mTORC1-bound TTI1 and TELO2 when they are phosphorylated by CK2 following growth factor deprivation, leading to their degradation. In contrast, the SCF(FBXO9) does not mediate ubiquitination of TTI1 and TELO2 when they are part of the mTORC2 complex. As a consequence, mTORC1 is inactivated to restrain cell growth and protein translation, while mTORC2 is activated due to the relief of feedback inhibition by mTORC1 (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-8951664 Neddylation
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
F-box only protein 9
Gene namesi
Name:Fbxo9
ORF Names:MNCb-2471
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1918788 Fbxo9

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001198871 – 437F-box only protein 9Add BLAST437
Isoform 2 (identifier: Q8BK06-2)
Initiator methionineiRemovedCombined sources
Isoform 3 (identifier: Q8BK06-3)
Initiator methionineiRemovedCombined sources

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei126PhosphoserineCombined sources1
Isoform 2 (identifier: Q8BK06-2)
Modified residuei2N-acetylalanineCombined sources1
Modified residuei10PhosphoserineCombined sources1
Isoform 3 (identifier: Q8BK06-3)
Modified residuei2N-acetylalanineCombined sources1
Modified residuei10PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ8BK06
PRIDEiQ8BK06

PTM databases

iPTMnetiQ8BK06
PhosphoSitePlusiQ8BK06

Expressioni

Gene expression databases

BgeeiENSMUSG00000001366 Expressed in 290 organ(s), highest expression level in blood
CleanExiMM_FBXO9
ExpressionAtlasiQ8BK06 baseline and differential
GenevisibleiQ8BK06 MM

Interactioni

Subunit structurei

Part of the SCF (SKP1-CUL1-F-box) E3 ubiquitin-protein ligase complex SCF(FBXO9) composed of CUL1, SKP1, RBX1 and FBXO9. Interacts with TTI1 and TELO2; when TTI1 and TELO2 are phosphorylated by CK2 (By similarity).By similarity

Protein-protein interaction databases

BioGridi214770, 1 interactor
STRINGi10090.ENSMUSP00000001402

Structurei

3D structure databases

ProteinModelPortaliQ8BK06
SMRiQ8BK06
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati84 – 117TPRAdd BLAST34
Domaini175 – 226F-boxPROSITE-ProRule annotationAdd BLAST52

Keywords - Domaini

TPR repeat

Phylogenomic databases

eggNOGiKOG2997 Eukaryota
ENOG410XPVI LUCA
GeneTreeiENSGT00390000014256
HOGENOMiHOG000047162
HOVERGENiHBG001041
InParanoidiQ8BK06
KOiK10295
OMAiSKTTYLR
OrthoDBiEOG091G0BDF
PhylomeDBiQ8BK06
TreeFamiTF324797

Family and domain databases

InterProiView protein in InterPro
IPR036047 F-box-like_dom_sf
IPR001810 F-box_dom
IPR036181 MIT_dom_sf
PfamiView protein in Pfam
PF12937 F-box-like, 1 hit
SUPFAMiSSF116846 SSF116846, 1 hit
SSF81383 SSF81383, 1 hit
PROSITEiView protein in PROSITE
PS50181 FBOX, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8BK06-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSAEAEEDCH SDADRVGDEG NESPAERDLQ AQLQMFRAQW MFELTPGVGS
60 70 80 90 100
SHGETRPCRA GRSSMLKAAA DTKGRQELAK EEKARELFLQ AVEEEQNGAL
110 120 130 140 150
YEAIKFYRRA MQLVPDIEFK ITYTRSPDGD GVGSGYIEEN EDASKMADLL
160 170 180 190 200
SYFQQQLTLQ ESVLKLCQPE LETSQTHISV LPMEVLMYIF RWVVSSDLDL
210 220 230 240 250
RSLEQLSLVC RGFYICARDP EIWRLACLKV WGRSCMKLVP YASWREMFLE
260 270 280 290 300
RPRVRFDGVY ISKTTYIRQG EQSLDGFYRA WHQVEYYRYM RFFPDGHVMM
310 320 330 340 350
LTTPEEPPSI VPRLRTRNTR TDAILLGHYR LSQDADNQTK VFAVITKKKE
360 370 380 390 400
EKPLDHKYRY FRRVPVQEAD HSFHVGLQLC SSGHQRFNKL IWIHHSCHIT
410 420 430
YKATGETAVS AFEIDKMYTP LLFARVRSYT AFSERPL
Note: No experimental confirmation available.
Length:437
Mass (Da):50,760
Last modified:March 1, 2003 - v1
Checksum:i8950C6F658F61FCB
GO
Isoform 2 (identifier: Q8BK06-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-2: MS → M

Show »
Length:436
Mass (Da):50,673
Checksum:i3479C6C144A26805
GO
Isoform 3 (identifier: Q8BK06-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-2: MS → M
     180-195: VLPMEVLMYIFRWVVS → GVAPWLLSLRNPWTSG
     196-437: Missing.

Note: No experimental confirmation available.Combined sources
Show »
Length:194
Mass (Da):21,721
Checksum:i64D572335E85D415
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E0CYX8E0CYX8_MOUSE
F-box only protein 9
Fbxo9
68Annotation score:

Sequence cautioni

The sequence BAA95068 differs from that shown. Reason: Frameshift at position 228.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti42F → C in BAB31231 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0129291 – 2MS → M in isoform 2 and isoform 3. 3 Publications2
Alternative sequenceiVSP_012930180 – 195VLPME…RWVVS → GVAPWLLSLRNPWTSG in isoform 3. 1 PublicationAdd BLAST16
Alternative sequenceiVSP_012931196 – 437Missing in isoform 3. 1 PublicationAdd BLAST242

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB041585 mRNA Translation: BAA95068.1 Frameshift.
AK077607 mRNA Translation: BAC36897.1
AK018482 mRNA Translation: BAB31231.1
BC020074 mRNA Translation: AAH20074.1
CCDSiCCDS52863.1 [Q8BK06-2]
CCDS52864.1 [Q8BK06-1]
RefSeqiNP_001074959.1, NM_001081490.2 [Q8BK06-1]
NP_076094.2, NM_023605.2 [Q8BK06-2]
UniGeneiMm.28584

Genome annotation databases

EnsembliENSMUST00000001402; ENSMUSP00000001402; ENSMUSG00000001366 [Q8BK06-1]
ENSMUST00000085311; ENSMUSP00000082419; ENSMUSG00000001366 [Q8BK06-2]
ENSMUST00000159099; ENSMUSP00000125381; ENSMUSG00000001366 [Q8BK06-3]
GeneIDi71538
KEGGimmu:71538
UCSCiuc033jlp.1 mouse [Q8BK06-2]
uc033jlr.1 mouse [Q8BK06-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB041585 mRNA Translation: BAA95068.1 Frameshift.
AK077607 mRNA Translation: BAC36897.1
AK018482 mRNA Translation: BAB31231.1
BC020074 mRNA Translation: AAH20074.1
CCDSiCCDS52863.1 [Q8BK06-2]
CCDS52864.1 [Q8BK06-1]
RefSeqiNP_001074959.1, NM_001081490.2 [Q8BK06-1]
NP_076094.2, NM_023605.2 [Q8BK06-2]
UniGeneiMm.28584

3D structure databases

ProteinModelPortaliQ8BK06
SMRiQ8BK06
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi214770, 1 interactor
STRINGi10090.ENSMUSP00000001402

PTM databases

iPTMnetiQ8BK06
PhosphoSitePlusiQ8BK06

Proteomic databases

PaxDbiQ8BK06
PRIDEiQ8BK06

Protocols and materials databases

DNASUi71538
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000001402; ENSMUSP00000001402; ENSMUSG00000001366 [Q8BK06-1]
ENSMUST00000085311; ENSMUSP00000082419; ENSMUSG00000001366 [Q8BK06-2]
ENSMUST00000159099; ENSMUSP00000125381; ENSMUSG00000001366 [Q8BK06-3]
GeneIDi71538
KEGGimmu:71538
UCSCiuc033jlp.1 mouse [Q8BK06-2]
uc033jlr.1 mouse [Q8BK06-1]

Organism-specific databases

CTDi26268
MGIiMGI:1918788 Fbxo9

Phylogenomic databases

eggNOGiKOG2997 Eukaryota
ENOG410XPVI LUCA
GeneTreeiENSGT00390000014256
HOGENOMiHOG000047162
HOVERGENiHBG001041
InParanoidiQ8BK06
KOiK10295
OMAiSKTTYLR
OrthoDBiEOG091G0BDF
PhylomeDBiQ8BK06
TreeFamiTF324797

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-MMU-8951664 Neddylation
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

ChiTaRSiFbxo9 mouse
PROiPR:Q8BK06
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000001366 Expressed in 290 organ(s), highest expression level in blood
CleanExiMM_FBXO9
ExpressionAtlasiQ8BK06 baseline and differential
GenevisibleiQ8BK06 MM

Family and domain databases

InterProiView protein in InterPro
IPR036047 F-box-like_dom_sf
IPR001810 F-box_dom
IPR036181 MIT_dom_sf
PfamiView protein in Pfam
PF12937 F-box-like, 1 hit
SUPFAMiSSF116846 SSF116846, 1 hit
SSF81383 SSF81383, 1 hit
PROSITEiView protein in PROSITE
PS50181 FBOX, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiFBX9_MOUSE
AccessioniPrimary (citable) accession number: Q8BK06
Secondary accession number(s): Q8VDY6, Q9D349, Q9JJC5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2003
Last modified: September 12, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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