UniProtKB - Q8BJQ9 (CGAT1_MOUSE)
Protein
Chondroitin sulfate N-acetylgalactosaminyltransferase 1
Gene
Csgalnact1
Organism
Mus musculus (Mouse)
Status
Functioni
Transfers 1,4-N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of glucuronic acid (GlcUA). Required for addition of the first GalNAc to the core tetrasaccharide linker and for elongation of chondroitin chains. Important role in chondroitin chain biosynthesis in cartilage formation, and subsequent endochondral ossification (PubMed:17145758, PubMed:21148564). Moreover, is involved in the metabolism of aggrecan (PubMed:21148564).2 Publications
Catalytic activityi
- 3-O-(β-D-GlcA-(1→3)-β-D-Gal-(1→3)-β-D-Gal-(1→4)-β-D-Xyl)-L-seryl-[protein] + UDP-N-acetyl-α-D-galactosamine = 3-O-(β-D-GalNAc-(1→4)-β-D-GlcA-(1→3)-β-D-Gal-(1→3)-β-D-Gal-(1→4)-β-D-Xyl)-L-seryl-[protein] + H+ + UDPBy similarityEC:2.4.1.174By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 358 | Divalent metal cationSequence analysis | 1 | |
Metal bindingi | 475 | Divalent metal cationSequence analysis | 1 |
GO - Molecular functioni
- acetylgalactosaminyltransferase activity Source: UniProtKB
- glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase activity Source: UniProtKB
- glucuronosyltransferase activity Source: UniProtKB
- glucuronylgalactosylproteoglycan 4-beta-N-acetylgalactosaminyltransferase activity Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
- peptidoglycan glycosyltransferase activity Source: UniProtKB
GO - Biological processi
- cartilage development Source: MGI
- chondroitin sulfate biosynthetic process Source: UniProtKB
- chondroitin sulfate metabolic process Source: MGI
- chondroitin sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process Source: UniProtKB
- endochondral ossification Source: MGI
- extracellular matrix organization Source: MGI
- UDP-glucuronate metabolic process Source: UniProtKB
- UDP-N-acetylgalactosamine metabolic process Source: UniProtKB
Keywordsi
Molecular function | Transferase |
Ligand | Metal-binding |
Enzyme and pathway databases
Reactomei | R-MMU-2022870, Chondroitin sulfate biosynthesis |
Protein family/group databases
CAZyi | GT7, Glycosyltransferase Family 7 |
Names & Taxonomyi
Protein namesi | Recommended name: Chondroitin sulfate N-acetylgalactosaminyltransferase 1Curated (EC:2.4.1.174By similarity)Short name: CsGalNAcT-1Curated Alternative name(s): Chondroitin beta-1,4-N-acetylgalactosaminyltransferase 1 Short name: Beta4GalNAcT-1 |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2442354, Csgalnact1 |
Subcellular locationi
Golgi apparatus
- Golgi stack membrane Curated; Single-pass type II membrane protein Curated
Golgi apparatus
- Golgi cisterna membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 12 | CytoplasmicSequence analysisAdd BLAST | 12 | |
Transmembranei | 13 – 33 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 21 | |
Topological domaini | 34 – 530 | LumenalSequence analysisAdd BLAST | 497 |
Keywords - Cellular componenti
Golgi apparatus, MembranePathology & Biotechi
Disruption phenotypei
Deficient mice are viable and fertile, but decreased body weight and length and abnormal cartilage (PubMed:21148564).1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000189565 | 1 – 530 | Chondroitin sulfate N-acetylgalactosaminyltransferase 1Add BLAST | 530 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 313 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 322 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
MaxQBi | Q8BJQ9 |
PaxDbi | Q8BJQ9 |
PRIDEi | Q8BJQ9 |
PTM databases
GlyGeni | Q8BJQ9, 2 sites |
iPTMneti | Q8BJQ9 |
PhosphoSitePlusi | Q8BJQ9 |
Expressioni
Developmental stagei
High expression in developing cartilage and during chondrocyte differentiation.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000036356, Expressed in blood and 273 other tissues |
ExpressionAtlasi | Q8BJQ9, baseline and differential |
Genevisiblei | Q8BJQ9, MM |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000077459 |
Miscellaneous databases
RNActi | Q8BJQ9, protein |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 57 – 93 | Sequence analysisAdd BLAST | 37 |
Sequence similaritiesi
Belongs to the chondroitin N-acetylgalactosaminyltransferase family.Curated
Keywords - Domaini
Coiled coil, Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3588, Eukaryota |
GeneTreei | ENSGT01010000222280 |
HOGENOMi | CLU_025958_0_1_1 |
InParanoidi | Q8BJQ9 |
OMAi | GIFHRWH |
OrthoDBi | 389974at2759 |
PhylomeDBi | Q8BJQ9 |
TreeFami | TF318303 |
Family and domain databases
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR008428, Chond_GalNAc IPR029044, Nucleotide-diphossugar_trans |
Pfami | View protein in Pfam PF05679, CHGN, 1 hit |
SUPFAMi | SSF53448, SSF53448, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q8BJQ9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVRRGLLGWI SRVVILLVLL CCAISVLYML ACTPKGDQEQ LGLPRANGPT
60 70 80 90 100
GKDGYQAVLQ EREEQHRNYV NSLKRQIAQL KDELQARSEQ FRSGQDQASD
110 120 130 140 150
ATSLRSGWDR EPKAQADLLA FLRGQVDKAE VHAGVKLATE YAAVPFDSFT
160 170 180 190 200
LQKVYQLETG LTRHPEEKPV RKDKRDELVE AIESALESLN SPVESSPHQR
210 220 230 240 250
PYTAADFIEG IYRTERDKGT LYELTFKGDH KHEFQRLVLF RPFGPIMKVK
260 270 280 290 300
KEKLNLANTL INVIVPLARR VDKFRHFMQN FREMCIQQDG RVHLTVVYFG
310 320 330 340 350
KEEMNEVKGI LENTSKAANF RNFTFIQLNG EFSRGKGLDV GARFWKGSNV
360 370 380 390 400
LLFFCDVDIY FTSEFLNTCR LNTQPGKKVF YPVLFSQYNP GVIYGHHDAV
410 420 430 440 450
PPLGQQLVIK KETGFWRDFG FGMTCQYRSD FINIGGFDLD IKGWGGEDVH
460 470 480 490 500
LYRKYLHSNL IVVRTPVRGL FHLWHEKHCM DELTPEQYKM CMQSKAMNEA
510 520 530
SHGQLGMLVF RHEIEAHLRK QKQKASSKKT
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0R4J1S7 | A0A0R4J1S7_MOUSE | Hexosyltransferase | Csgalnact1 | 300 | Annotation score: |
Sequence cautioni
The sequence BAE25692 differs from that shown. Reason: Erroneous termination. Truncated C-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 163 | R → P in BAE25692 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 186 | L → M in BAC26066 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 189 | L → V in BAC28340 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 393 | I → V in AAH89162 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK028688 mRNA Translation: BAC26066.1 AK033522 mRNA Translation: BAC28340.1 AK049770 mRNA Translation: BAC33912.1 AK080687 mRNA Translation: BAC37982.1 AK133567 mRNA Translation: BAE21729.1 AK144089 mRNA Translation: BAE25692.1 Sequence problems. BC026599 mRNA Translation: AAH26599.1 BC089162 mRNA Translation: AAH89162.1 |
CCDSi | CCDS22341.1 |
RefSeqi | NP_001239552.1, NM_001252623.1 NP_766341.4, NM_172753.5 XP_006509687.1, XM_006509624.3 XP_006509688.1, XM_006509625.3 XP_006509689.1, XM_006509626.1 XP_006509690.1, XM_006509627.3 XP_006509691.1, XM_006509628.3 XP_006509692.1, XM_006509629.1 XP_006509693.1, XM_006509630.3 XP_011240592.1, XM_011242290.2 |
Genome annotation databases
Ensembli | ENSMUST00000078350; ENSMUSP00000077459; ENSMUSG00000036356 ENSMUST00000130214; ENSMUSP00000119817; ENSMUSG00000036356 |
GeneIDi | 234356 |
KEGGi | mmu:234356 |
UCSCi | uc009lwf.3, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK028688 mRNA Translation: BAC26066.1 AK033522 mRNA Translation: BAC28340.1 AK049770 mRNA Translation: BAC33912.1 AK080687 mRNA Translation: BAC37982.1 AK133567 mRNA Translation: BAE21729.1 AK144089 mRNA Translation: BAE25692.1 Sequence problems. BC026599 mRNA Translation: AAH26599.1 BC089162 mRNA Translation: AAH89162.1 |
CCDSi | CCDS22341.1 |
RefSeqi | NP_001239552.1, NM_001252623.1 NP_766341.4, NM_172753.5 XP_006509687.1, XM_006509624.3 XP_006509688.1, XM_006509625.3 XP_006509689.1, XM_006509626.1 XP_006509690.1, XM_006509627.3 XP_006509691.1, XM_006509628.3 XP_006509692.1, XM_006509629.1 XP_006509693.1, XM_006509630.3 XP_011240592.1, XM_011242290.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000077459 |
Protein family/group databases
CAZyi | GT7, Glycosyltransferase Family 7 |
PTM databases
GlyGeni | Q8BJQ9, 2 sites |
iPTMneti | Q8BJQ9 |
PhosphoSitePlusi | Q8BJQ9 |
Proteomic databases
MaxQBi | Q8BJQ9 |
PaxDbi | Q8BJQ9 |
PRIDEi | Q8BJQ9 |
Protocols and materials databases
Antibodypediai | 22388, 205 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000078350; ENSMUSP00000077459; ENSMUSG00000036356 ENSMUST00000130214; ENSMUSP00000119817; ENSMUSG00000036356 |
GeneIDi | 234356 |
KEGGi | mmu:234356 |
UCSCi | uc009lwf.3, mouse |
Organism-specific databases
CTDi | 55790 |
MGIi | MGI:2442354, Csgalnact1 |
Phylogenomic databases
eggNOGi | KOG3588, Eukaryota |
GeneTreei | ENSGT01010000222280 |
HOGENOMi | CLU_025958_0_1_1 |
InParanoidi | Q8BJQ9 |
OMAi | GIFHRWH |
OrthoDBi | 389974at2759 |
PhylomeDBi | Q8BJQ9 |
TreeFami | TF318303 |
Enzyme and pathway databases
Reactomei | R-MMU-2022870, Chondroitin sulfate biosynthesis |
Miscellaneous databases
BioGRID-ORCSi | 234356, 1 hit in 17 CRISPR screens |
ChiTaRSi | Csgalnact1, mouse |
PROi | PR:Q8BJQ9 |
RNActi | Q8BJQ9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000036356, Expressed in blood and 273 other tissues |
ExpressionAtlasi | Q8BJQ9, baseline and differential |
Genevisiblei | Q8BJQ9, MM |
Family and domain databases
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR008428, Chond_GalNAc IPR029044, Nucleotide-diphossugar_trans |
Pfami | View protein in Pfam PF05679, CHGN, 1 hit |
SUPFAMi | SSF53448, SSF53448, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CGAT1_MOUSE | |
Accessioni | Q8BJQ9Primary (citable) accession number: Q8BJQ9 Secondary accession number(s): Q3UNQ5 Q8R0M6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 2005 |
Last sequence update: | March 1, 2003 | |
Last modified: | December 2, 2020 | |
This is version 132 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families