UniProtKB - Q8BJQ4 (TCAM2_MOUSE)
TIR domain-containing adapter molecule 2
Ticam2
Functioni
GO - Molecular functioni
- interleukin-1 receptor binding Source: MGI
- phospholipid binding Source: UniProtKB
- protein heterodimerization activity Source: MGI
GO - Biological processi
- cellular response to lipopolysaccharide Source: UniProtKB
- defense response to virus Source: MGI
- endoplasmic reticulum to Golgi vesicle-mediated transport Source: GO_Central
- Golgi organization Source: GO_Central
- inflammatory response Source: UniProtKB-KW
- innate immune response Source: UniProtKB-KW
- intracellular protein transport Source: GO_Central
- negative regulation of chemokine (C-C motif) ligand 5 production Source: MGI
- negative regulation of toll-like receptor 4 signaling pathway Source: MGI
- phagocytosis Source: UniProtKB
- positive regulation of chemokine (C-C motif) ligand 5 production Source: UniProtKB
- positive regulation of interferon-gamma production Source: UniProtKB
- positive regulation of interleukin-18-mediated signaling pathway Source: UniProtKB
- positive regulation of interleukin-6 production Source: UniProtKB
- positive regulation of toll-like receptor 4 signaling pathway Source: UniProtKB
- regulation of cytokine production Source: MGI
- regulation of I-kappaB kinase/NF-kappaB signaling Source: MGI
- response to interleukin-12 Source: UniProtKB
- TRAM-dependent toll-like receptor 4 signaling pathway Source: UniProtKB
Keywordsi
Biological process | Immunity, Inflammatory response, Innate immunity |
Enzyme and pathway databases
Reactomei | R-MMU-140534, Caspase activation via Death Receptors in the presence of ligand R-MMU-166016, Toll Like Receptor 4 (TLR4) Cascade R-MMU-166166, MyD88-independent TLR4 cascade R-MMU-2562578, TRIF-mediated programmed cell death R-MMU-6798695, Neutrophil degranulation R-MMU-936964, Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon R-MMU-937041, IKK complex recruitment mediated by RIP1 R-MMU-937072, TRAF6-mediated induction of TAK1 complex within TLR4 complex R-MMU-975163, IRAK2 mediated activation of TAK1 complex upon TLR7/8 or 9 stimulation |
Names & Taxonomyi
Protein namesi | Recommended name: TIR domain-containing adapter molecule 2Short name: TICAM-2 Alternative name(s): TRIF-related adapter molecule Toll/interleukin-1 receptor domain-containing protein |
Gene namesi | Name:Ticam2 Synonyms:Tirp, Tram |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:3040056, Ticam2 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Plasma membrane
- Cell membrane 1 Publication
Endoplasmic reticulum
- Endoplasmic reticulum By similarity
Endosome
- Early endosome 1 Publication
- Late endosome By similarity
Golgi apparatus
- Golgi apparatus By similarity
Other locations
- phagocytic cup By similarity
Note: Localized to the plasma membrane as a result of myristoylation.By similarity
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB
Endosome
- early endosome Source: UniProtKB
- endosome Source: UniProtKB
- late endosome Source: UniProtKB
Golgi apparatus
- Golgi apparatus Source: GO_Central
Plasma Membrane
- interleukin-1 receptor complex Source: MGI
- phagocytic cup Source: MGI
- plasma membrane Source: UniProtKB
Other locations
- cell projection Source: UniProtKB-KW
- COPII-coated ER to Golgi transport vesicle Source: GO_Central
- endoplasmic reticulum-Golgi intermediate compartment Source: GO_Central
Keywords - Cellular componenti
Cell membrane, Cell projection, Cytoplasm, Endoplasmic reticulum, Endosome, Golgi apparatus, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 2 | G → A: Results in relocalization from membrane to cytosol; impairs ability to promote LPS-induced IL6 and CCL5 production. 1 Publication | 1 | |
Mutagenesisi | 7 | K → E: Results in exclusive localization to early endosomes and no effect on ability to promote LPS-induced IL6 and CCL5 production; when associated with E-15 and E-17. 1 Publication | 1 | |
Mutagenesisi | 15 | W → E: Results in exclusive localization to early endosomes and no effect on ability to promote LPS-induced IL6 and CCL5 production; when associated with E-7 and E-17. 1 Publication | 1 | |
Mutagenesisi | 17 | K → E: Results in exclusive localization to early endosomes and no effect on ability to promote LPS-induced IL6 and CCL5 production; when associated with E-7 and E-15. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000317690 | 2 – 232 | TIR domain-containing adapter molecule 2Add BLAST | 231 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycineBy similarity | 1 | |
Modified residuei | 164 | PhosphotyrosineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Lipoprotein, Myristate, PhosphoproteinProteomic databases
MaxQBi | Q8BJQ4 |
PaxDbi | Q8BJQ4 |
PRIDEi | Q8BJQ4 |
ProteomicsDBi | 263087 |
PTM databases
iPTMneti | Q8BJQ4 |
PhosphoSitePlusi | Q8BJQ4 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000056130, Expressed in bone marrow macrophage and 46 other tissues |
Interactioni
Subunit structurei
Homodimer.
Interacts with TLR4, TICAM1, IRF3 and IRF7 in response to LPS.
Interacts with IL1R1, IL1RAP, IRAK2, IRAK3 and TRAF6.
Interacts with protein kinase-inactive mutants of IRAK1 and IRAK4.
Isoform 1 interacts with isoform 2; the interaction occurs in late endosomes and disrupts the interaction between isoform 1 and TICAM1.
Interacts with MYD88; the interaction decreases after IL-18 stimulation in a time-dependent manner.
Interacts with IL18R1 and IL18RAP.
Interacts with TLR2.
Interacts with RAB11FIP2.
By similarityGO - Molecular functioni
- interleukin-1 receptor binding Source: MGI
- protein heterodimerization activity Source: MGI
Protein-protein interaction databases
BioGRIDi | 230399, 7 interactors |
IntActi | Q8BJQ4, 1 interactor |
STRINGi | 10090.ENSMUSP00000066239 |
Miscellaneous databases
RNActi | Q8BJQ4, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 74 – 226 | TIRPROSITE-ProRule annotationAdd BLAST | 153 |
Domaini
Phylogenomic databases
eggNOGi | ENOG502S2I6, Eukaryota |
GeneTreei | ENSGT00940000164712 |
HOGENOMi | CLU_094608_0_0_1 |
InParanoidi | Q8BJQ4 |
OMAi | SAWTIIL |
OrthoDBi | 1351613at2759 |
PhylomeDBi | Q8BJQ4 |
TreeFami | TF336953 |
Family and domain databases
Gene3Di | 3.40.50.10140, 1 hit |
InterProi | View protein in InterPro IPR015720, Emp24-like IPR000157, TIR_dom IPR035897, Toll_tir_struct_dom_sf |
PANTHERi | PTHR22811, PTHR22811, 1 hit |
Pfami | View protein in Pfam PF13676, TIR_2, 1 hit |
SUPFAMi | SSF52200, SSF52200, 1 hit |
PROSITEi | View protein in PROSITE PS50104, TIR, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MGVGKSKLDK CPLSWHKKDS VDADQDGHES DSKNSEEACL RGFVEQSSGS
60 70 80 90 100
EPPTGEQDQP EAKGAGPEEQ DEEEFLKFVI LHAEDDTDEA LRVQDLLQND
110 120 130 140 150
FGIRPGIVFA EMPCGRLHLQ NLDDAVNGSA WTILLLTENF LRDTWCNFQF
160 170 180 190 200
YTSLMNSVSR QHKYNSVIPM RPLNSPLPRE RTPLALQTIN ALEEESQGFS
210 220 230
TQVERIFRES VFERQQSIWK ETRSVSQKQF IA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 185 | A → T in BAE30771 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB100441 mRNA Translation: BAC98398.1 AY275837 mRNA Translation: AAP81749.1 AY268050 mRNA Translation: AAP29979.1 AK080873 mRNA Translation: BAC38055.1 AK151885 mRNA Translation: BAE30771.1 AK134103 mRNA Translation: BAE22014.1 BC099933 mRNA Translation: AAH99933.1 |
CCDSi | CCDS29232.1 |
RefSeqi | NP_775570.1, NM_173394.3 |
Genome annotation databases
Ensembli | ENSMUST00000070084; ENSMUSP00000066239; ENSMUSG00000056130 |
GeneIDi | 225471 |
KEGGi | mmu:225471 |
UCSCi | uc008evp.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB100441 mRNA Translation: BAC98398.1 AY275837 mRNA Translation: AAP81749.1 AY268050 mRNA Translation: AAP29979.1 AK080873 mRNA Translation: BAC38055.1 AK151885 mRNA Translation: BAE30771.1 AK134103 mRNA Translation: BAE22014.1 BC099933 mRNA Translation: AAH99933.1 |
CCDSi | CCDS29232.1 |
RefSeqi | NP_775570.1, NM_173394.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 230399, 7 interactors |
IntActi | Q8BJQ4, 1 interactor |
STRINGi | 10090.ENSMUSP00000066239 |
PTM databases
iPTMneti | Q8BJQ4 |
PhosphoSitePlusi | Q8BJQ4 |
Proteomic databases
MaxQBi | Q8BJQ4 |
PaxDbi | Q8BJQ4 |
PRIDEi | Q8BJQ4 |
ProteomicsDBi | 263087 |
Genome annotation databases
Ensembli | ENSMUST00000070084; ENSMUSP00000066239; ENSMUSG00000056130 |
GeneIDi | 225471 |
KEGGi | mmu:225471 |
UCSCi | uc008evp.2, mouse |
Organism-specific databases
CTDi | 353376 |
MGIi | MGI:3040056, Ticam2 |
Phylogenomic databases
eggNOGi | ENOG502S2I6, Eukaryota |
GeneTreei | ENSGT00940000164712 |
HOGENOMi | CLU_094608_0_0_1 |
InParanoidi | Q8BJQ4 |
OMAi | SAWTIIL |
OrthoDBi | 1351613at2759 |
PhylomeDBi | Q8BJQ4 |
TreeFami | TF336953 |
Enzyme and pathway databases
Reactomei | R-MMU-140534, Caspase activation via Death Receptors in the presence of ligand R-MMU-166016, Toll Like Receptor 4 (TLR4) Cascade R-MMU-166166, MyD88-independent TLR4 cascade R-MMU-2562578, TRIF-mediated programmed cell death R-MMU-6798695, Neutrophil degranulation R-MMU-936964, Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon R-MMU-937041, IKK complex recruitment mediated by RIP1 R-MMU-937072, TRAF6-mediated induction of TAK1 complex within TLR4 complex R-MMU-975163, IRAK2 mediated activation of TAK1 complex upon TLR7/8 or 9 stimulation |
Miscellaneous databases
BioGRID-ORCSi | 225471, 0 hits in 52 CRISPR screens |
ChiTaRSi | Ticam2, mouse |
PROi | PR:Q8BJQ4 |
RNActi | Q8BJQ4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000056130, Expressed in bone marrow macrophage and 46 other tissues |
Family and domain databases
Gene3Di | 3.40.50.10140, 1 hit |
InterProi | View protein in InterPro IPR015720, Emp24-like IPR000157, TIR_dom IPR035897, Toll_tir_struct_dom_sf |
PANTHERi | PTHR22811, PTHR22811, 1 hit |
Pfami | View protein in Pfam PF13676, TIR_2, 1 hit |
SUPFAMi | SSF52200, SSF52200, 1 hit |
PROSITEi | View protein in PROSITE PS50104, TIR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TCAM2_MOUSE | |
Accessioni | Q8BJQ4Primary (citable) accession number: Q8BJQ4 Secondary accession number(s): Q3U996 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 5, 2008 |
Last sequence update: | March 1, 2003 | |
Last modified: | April 7, 2021 | |
This is version 140 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot