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UniProtKB - Q8BJL0 (SMAL1_MOUSE)

Protein

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1

Gene

Smarcal1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATP-dependent annealing helicase that binds selectively to fork DNA relative to ssDNA or dsDNA and catalyzes the rewinding of the stably unwound DNA. Rewinds single-stranded DNA bubbles that are stably bound by replication protein A (RPA). Acts throughout the genome to reanneal stably unwound DNA, performing the opposite reaction of many enzymes, such as helicases and polymerases, that unwind DNA. May play an important role in DNA damage response by acting at stalled replication forks (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi417 – 424ATPPROSITE-ProRule annotation8

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionHelicase, Hydrolase
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1 (EC:3.6.4.-)
Alternative name(s):
HepA-related protein
Short name:
mharp
Sucrose nonfermenting protein 2-like 1
Gene namesi
Name:Smarcal1
Synonyms:Harp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1859183 Smarcal1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000743492 – 910SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1Add BLAST909

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei118PhosphoserineBy similarity1
Modified residuei165PhosphoserineBy similarity1

Post-translational modificationi

DNA damage-regulated phosphorylation by kinases that may include ATM, ATR and PRKDC.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ8BJL0
PeptideAtlasiQ8BJL0
PRIDEiQ8BJL0

PTM databases

iPTMnetiQ8BJL0
PhosphoSitePlusiQ8BJL0

Expressioni

Tissue specificityi

Ubiquitously expressed, with high levels in brain, heart, kidney, liver and testis.1 Publication

Gene expression databases

BgeeiENSMUSG00000039354 Expressed in 263 organ(s), highest expression level in oocyte
ExpressionAtlasiQ8BJL0 baseline and differential
GenevisibleiQ8BJL0 MM

Interactioni

Subunit structurei

Interacts with RPA2; the interaction is direct and mediates the recruitment by the RPA complex of SMARCAL1 to sites of DNA damage.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000047589

Structurei

Secondary structure

1910
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ8BJL0
SMRiQ8BJL0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini199 – 269HARP 1PROSITE-ProRule annotationAdd BLAST71
Domaini286 – 357HARP 2PROSITE-ProRule annotationAdd BLAST72
Domaini404 – 559Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST156
Domaini674 – 827Helicase C-terminalPROSITE-ProRule annotationAdd BLAST154

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 29Mediates interaction with RPA2By similarityAdd BLAST28

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili3 – 34Sequence analysisAdd BLAST32

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi508 – 511DESH box4

Sequence similaritiesi

Belongs to the SNF2/RAD54 helicase family. SMARCAL1 subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG1000 Eukaryota
COG0553 LUCA
GeneTreeiENSGT00910000144109
HOVERGENiHBG054110
InParanoidiQ8BJL0
KOiK14440
OMAiKTRKWNF
OrthoDBiEOG091G0A1B
PhylomeDBiQ8BJL0
TreeFamiTF106474

Family and domain databases

CDDicd00079 HELICc, 1 hit
Gene3Di3.40.50.10810, 1 hit
InterProiView protein in InterPro
IPR030101 HARP(SMARCAL1)
IPR010003 HARP_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR038718 SNF2-like_sf
IPR000330 SNF2_N
PANTHERiPTHR10799:SF731 PTHR10799:SF731, 2 hits
PfamiView protein in Pfam
PF07443 HARP, 2 hits
PF00271 Helicase_C, 1 hit
PF00176 SNF2_N, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS51467 HARP, 2 hits
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8BJL0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSLPLTEEQR KKIEENRQKA LARRAEKLSE QPQSAASGSS AAGPSQSKQG 
        60         70         80         90        100
SLLNLLAEPS KPVGHASIFK QQNLSNSFPT DQRPHSSRCS QPSPAEETTG 
       110        120        130        140        150
LWKTQGEMST ACPKPNPSPP GASNQPLLGY KSSEGQPQAT WDTGASSSGP 
       160        170        180        190        200
FPRDPELEAK AARPSTSRQS ISDSFYVLGG KTPRTEGRPP NILQTTPQNT 
       210        220        230        240        250
GFLRGACIKT GDRFRVKIGY NQELIAVFKS LPSRHYDSFT KTWDFSMSDY 
       260        270        280        290        300
RALMKAVERL STVSLKPLDE AGGSVGGQTS LPSAPSLTFV TGKCMLISRV 
       310        320        330        340        350
RFEVDIGYSE AVIGLFKQME SRSYDIKTRK WSFLLEEHNK LIARSRELKQ 
       360        370        380        390        400
VQLDPLPKTV TLAFASQLEK TSPKLKADVP EADLSGVDAK LVSSLMPFQR 
       410        420        430        440        450
EGVSFAISKR GRLLLADDMG LGKTVQAICI AAFYRKEWPL LVVVPSSVRF 
       460        470        480        490        500
TWEQAFLRWL PSLSPENINV VVTGKGRLTA GLVNIVSFDL LCKLERQLKT 
       510        520        530        540        550
PFKVVIIDES HFLKNIKTAR CRAAVPILKV AKRVILLSGT PAMSRPAELY 
       560        570        580        590        600
TQIIAVKPTF FPQFHAFGLR YCDAKRLPWG WDYSGSSNLG ELKLLLEEAI 
       610        620        630        640        650
MLRRLKSDVL SQLPAKQRKM VVVNPGRISS RAKAALDAAA KEMTKDKTKQ 
       660        670        680        690        700
QQKEALLVFF NRTAEAKIPC VVEYILDLLD SGREKFLVFA HHKVILDAVA 
       710        720        730        740        750
KELERKNVQH IRIDGSTPSA DREAQCQRFQ LSKGHTVALL SITAANMGLT 
       760        770        780        790        800
FSTADLVVFA ELFWNPGVLI QAEDRVHRIG QTNSVSIHYL VAKGTADDYL 
       810        820        830        840        850
WPLIQEKIKV LGEAGLSETN FSEMTEATDY VHKDPKQKTI YDLFQQSFED 
       860        870        880        890        900
DGNDMEFLEA AESFELGSTS GTSGNISQDL GDLLDEDEGS PPKKSRFEFF 
       910
DNWDSFSSPF
Length:910
Mass (Da):100,840
Last modified:March 1, 2003 - v1
Checksum:iF6B28F847BD6FC18
GO
Isoform 2 (identifier: Q8BJL0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     769-788: LIQAEDRVHRIGQTNSVSIH → GNVARVPLGMPRAEKYKIRK
     789-910: Missing.

Note: May be due to an intron retention.
Show »
Length:788
Mass (Da):87,126
Checksum:iAE70BAB0A799A3F8
GO
Isoform 3 (identifier: Q8BJL0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     508-574: DESHFLKNIK...HAFGLRYCDA → VSNGIALKYF...QPLSLCARLS
     575-910: Missing.

Note: No experimental confirmation available.
Show »
Length:574
Mass (Da):63,218
Checksum:i0E8930A229E115E8
GO
Isoform 4 (identifier: Q8BJL0-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     404-404: S → R
     405-910: Missing.

Note: No experimental confirmation available.
Show »
Length:404
Mass (Da):44,190
Checksum:i92E5249A242D75A6
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3YUX5D3YUX5_MOUSE
SWI/SNF-related matrix-associated a...
Smarcal1
194Annotation score:
D3Z0J8D3Z0J8_MOUSE
SWI/SNF-related matrix-associated a...
Smarcal1
98Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti35A → T in AAH29078 (PubMed:15489334).Curated1
Sequence conflicti78F → L in AAH29078 (PubMed:15489334).Curated1
Sequence conflicti529K → N in BAE32320 (PubMed:16141072).Curated1
Sequence conflicti685K → R in BAC31469 (PubMed:16141072).Curated1
Sequence conflicti717 – 725Missing in AAG47648 (PubMed:10857751).Curated9
Sequence conflicti717T → R in AAF24985 (PubMed:10857751).Curated1
Sequence conflicti719 – 721SAD → TRA in AAF24985 (PubMed:10857751).Curated3
Sequence conflicti724 – 725AQ → LK in AAF24985 (PubMed:10857751).Curated2
Sequence conflicti743T → P in AAF24985 (PubMed:10857751).Curated1
Sequence conflicti834D → A in AAF24985 (PubMed:10857751).Curated1
Sequence conflicti834Missing in AAG47648 (PubMed:10857751).Curated1
Sequence conflicti866 – 868LGS → IKN in AAF24985 (PubMed:10857751).Curated3

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_036216404S → R in isoform 4. 1 Publication1
Alternative sequenceiVSP_036217405 – 910Missing in isoform 4. 1 PublicationAdd BLAST506
Alternative sequenceiVSP_036218508 – 574DESHF…RYCDA → VSNGIALKYFVCLDTRKGST DLGICVLLGPLWALRREGNR NRCLSFIENDFFIPFLKQPL SLCARLS in isoform 3. 1 PublicationAdd BLAST67
Alternative sequenceiVSP_036219575 – 910Missing in isoform 3. 1 PublicationAdd BLAST336
Alternative sequenceiVSP_012919769 – 788LIQAE…SVSIH → GNVARVPLGMPRAEKYKIRK in isoform 2. 1 PublicationAdd BLAST20
Alternative sequenceiVSP_012920789 – 910Missing in isoform 2. 1 PublicationAdd BLAST122

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF088884 mRNA Translation: AAF24985.1
AF209773 Genomic DNA Translation: AAG47648.1
AK042332 mRNA Translation: BAE20630.1
AK043129 mRNA Translation: BAC31469.1
AK077878 mRNA Translation: BAC37047.1
AK083488 mRNA Translation: BAC38933.1
AK154020 mRNA Translation: BAE32320.1
AK169465 mRNA Translation: BAE41189.1
BC029078 mRNA Translation: AAH29078.1
CCDSiCCDS35609.1 [Q8BJL0-1]
RefSeqiNP_061287.2, NM_018817.2 [Q8BJL0-1]
XP_006496204.1, XM_006496141.3 [Q8BJL0-1]
XP_006496205.1, XM_006496142.3 [Q8BJL0-1]
XP_006496206.1, XM_006496143.3 [Q8BJL0-1]
XP_006496207.1, XM_006496144.1 [Q8BJL0-1]
UniGeneiMm.274232

Genome annotation databases

EnsembliENSMUST00000047615; ENSMUSP00000047589; ENSMUSG00000039354 [Q8BJL0-1]
ENSMUST00000152225; ENSMUSP00000137833; ENSMUSG00000039354 [Q8BJL0-1]
GeneIDi54380
KEGGimmu:54380
UCSCiuc007bko.2 mouse [Q8BJL0-4]
uc007bkq.2 mouse [Q8BJL0-3]
uc007bkr.2 mouse [Q8BJL0-2]
uc007bks.2 mouse [Q8BJL0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF088884 mRNA Translation: AAF24985.1
AF209773 Genomic DNA Translation: AAG47648.1
AK042332 mRNA Translation: BAE20630.1
AK043129 mRNA Translation: BAC31469.1
AK077878 mRNA Translation: BAC37047.1
AK083488 mRNA Translation: BAC38933.1
AK154020 mRNA Translation: BAE32320.1
AK169465 mRNA Translation: BAE41189.1
BC029078 mRNA Translation: AAH29078.1
CCDSiCCDS35609.1 [Q8BJL0-1]
RefSeqiNP_061287.2, NM_018817.2 [Q8BJL0-1]
XP_006496204.1, XM_006496141.3 [Q8BJL0-1]
XP_006496205.1, XM_006496142.3 [Q8BJL0-1]
XP_006496206.1, XM_006496143.3 [Q8BJL0-1]
XP_006496207.1, XM_006496144.1 [Q8BJL0-1]
UniGeneiMm.274232

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4O66X-ray1.90A/B/C/D197-268[»]
ProteinModelPortaliQ8BJL0
SMRiQ8BJL0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000047589

PTM databases

iPTMnetiQ8BJL0
PhosphoSitePlusiQ8BJL0

Proteomic databases

PaxDbiQ8BJL0
PeptideAtlasiQ8BJL0
PRIDEiQ8BJL0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047615; ENSMUSP00000047589; ENSMUSG00000039354 [Q8BJL0-1]
ENSMUST00000152225; ENSMUSP00000137833; ENSMUSG00000039354 [Q8BJL0-1]
GeneIDi54380
KEGGimmu:54380
UCSCiuc007bko.2 mouse [Q8BJL0-4]
uc007bkq.2 mouse [Q8BJL0-3]
uc007bkr.2 mouse [Q8BJL0-2]
uc007bks.2 mouse [Q8BJL0-1]

Organism-specific databases

CTDi50485
MGIiMGI:1859183 Smarcal1

Phylogenomic databases

eggNOGiKOG1000 Eukaryota
COG0553 LUCA
GeneTreeiENSGT00910000144109
HOVERGENiHBG054110
InParanoidiQ8BJL0
KOiK14440
OMAiKTRKWNF
OrthoDBiEOG091G0A1B
PhylomeDBiQ8BJL0
TreeFamiTF106474

Miscellaneous databases

ChiTaRSiSmarcal1 mouse
PROiPR:Q8BJL0
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000039354 Expressed in 263 organ(s), highest expression level in oocyte
ExpressionAtlasiQ8BJL0 baseline and differential
GenevisibleiQ8BJL0 MM

Family and domain databases

CDDicd00079 HELICc, 1 hit
Gene3Di3.40.50.10810, 1 hit
InterProiView protein in InterPro
IPR030101 HARP(SMARCAL1)
IPR010003 HARP_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR038718 SNF2-like_sf
IPR000330 SNF2_N
PANTHERiPTHR10799:SF731 PTHR10799:SF731, 2 hits
PfamiView protein in Pfam
PF07443 HARP, 2 hits
PF00271 Helicase_C, 1 hit
PF00176 SNF2_N, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS51467 HARP, 2 hits
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSMAL1_MOUSE
AccessioniPrimary (citable) accession number: Q8BJL0
Secondary accession number(s): Q3TEQ9
, Q3U4W0, Q3V3A8, Q8BVK7, Q8BXW4, Q8K309, Q9EQK8, Q9QYC4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2003
Last modified: November 7, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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