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Protein

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1

Gene

Smarcal1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATP-dependent annealing helicase that binds selectively to fork DNA relative to ssDNA or dsDNA and catalyzes the rewinding of the stably unwound DNA. Rewinds single-stranded DNA bubbles that are stably bound by replication protein A (RPA). Acts throughout the genome to reanneal stably unwound DNA, performing the opposite reaction of many enzymes, such as helicases and polymerases, that unwind DNA. May play an important role in DNA damage response by acting at stalled replication forks (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi417 – 424ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHelicase, Hydrolase
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1 (EC:3.6.4.-)
Alternative name(s):
HepA-related protein
Short name:
mharp
Sucrose nonfermenting protein 2-like 1
Gene namesi
Name:Smarcal1
Synonyms:Harp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1859183 Smarcal1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000743492 – 910SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1Add BLAST909

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei118PhosphoserineBy similarity1
Modified residuei165PhosphoserineBy similarity1

Post-translational modificationi

DNA damage-regulated phosphorylation by kinases that may include ATM, ATR and PRKDC.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ8BJL0
PeptideAtlasiQ8BJL0
PRIDEiQ8BJL0

PTM databases

iPTMnetiQ8BJL0
PhosphoSitePlusiQ8BJL0

Expressioni

Tissue specificityi

Ubiquitously expressed, with high levels in brain, heart, kidney, liver and testis.1 Publication

Gene expression databases

BgeeiENSMUSG00000039354
ExpressionAtlasiQ8BJL0 baseline and differential
GenevisibleiQ8BJL0 MM

Interactioni

Subunit structurei

Interacts with RPA2; the interaction is direct and mediates the recruitment by the RPA complex of SMARCAL1 to sites of DNA damage.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000047589

Structurei

Secondary structure

1910
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi203 – 210Combined sources8
Beta strandi213 – 218Combined sources6
Helixi222 – 230Combined sources9
Beta strandi235 – 237Combined sources3
Turni238 – 241Combined sources4
Beta strandi242 – 246Combined sources5
Helixi247 – 249Combined sources3
Helixi250 – 257Combined sources8
Beta strandi263 – 266Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4O66X-ray1.90A/B/C/D197-268[»]
ProteinModelPortaliQ8BJL0
SMRiQ8BJL0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini199 – 269HARP 1PROSITE-ProRule annotationAdd BLAST71
Domaini286 – 357HARP 2PROSITE-ProRule annotationAdd BLAST72
Domaini404 – 559Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST156
Domaini674 – 827Helicase C-terminalPROSITE-ProRule annotationAdd BLAST154

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 29Mediates interaction with RPA2By similarityAdd BLAST28

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili3 – 34Sequence analysisAdd BLAST32

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi508 – 511DESH box4

Sequence similaritiesi

Belongs to the SNF2/RAD54 helicase family. SMARCAL1 subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG1000 Eukaryota
COG0553 LUCA
GeneTreeiENSGT00910000144109
HOVERGENiHBG054110
InParanoidiQ8BJL0
KOiK14440
OMAiANMGLTF
OrthoDBiEOG091G0A1B
PhylomeDBiQ8BJL0
TreeFamiTF106474

Family and domain databases

CDDicd00079 HELICc, 1 hit
Gene3Di3.40.50.10810, 1 hit
InterProiView protein in InterPro
IPR030101 HARP(SMARCAL1)
IPR010003 HARP_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR038718 SNF2-like_sf
IPR000330 SNF2_N
PANTHERiPTHR10799:SF731 PTHR10799:SF731, 2 hits
PfamiView protein in Pfam
PF07443 HARP, 2 hits
PF00271 Helicase_C, 1 hit
PF00176 SNF2_N, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 3 hits
PROSITEiView protein in PROSITE
PS51467 HARP, 2 hits
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BJL0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLPLTEEQR KKIEENRQKA LARRAEKLSE QPQSAASGSS AAGPSQSKQG
60 70 80 90 100
SLLNLLAEPS KPVGHASIFK QQNLSNSFPT DQRPHSSRCS QPSPAEETTG
110 120 130 140 150
LWKTQGEMST ACPKPNPSPP GASNQPLLGY KSSEGQPQAT WDTGASSSGP
160 170 180 190 200
FPRDPELEAK AARPSTSRQS ISDSFYVLGG KTPRTEGRPP NILQTTPQNT
210 220 230 240 250
GFLRGACIKT GDRFRVKIGY NQELIAVFKS LPSRHYDSFT KTWDFSMSDY
260 270 280 290 300
RALMKAVERL STVSLKPLDE AGGSVGGQTS LPSAPSLTFV TGKCMLISRV
310 320 330 340 350
RFEVDIGYSE AVIGLFKQME SRSYDIKTRK WSFLLEEHNK LIARSRELKQ
360 370 380 390 400
VQLDPLPKTV TLAFASQLEK TSPKLKADVP EADLSGVDAK LVSSLMPFQR
410 420 430 440 450
EGVSFAISKR GRLLLADDMG LGKTVQAICI AAFYRKEWPL LVVVPSSVRF
460 470 480 490 500
TWEQAFLRWL PSLSPENINV VVTGKGRLTA GLVNIVSFDL LCKLERQLKT
510 520 530 540 550
PFKVVIIDES HFLKNIKTAR CRAAVPILKV AKRVILLSGT PAMSRPAELY
560 570 580 590 600
TQIIAVKPTF FPQFHAFGLR YCDAKRLPWG WDYSGSSNLG ELKLLLEEAI
610 620 630 640 650
MLRRLKSDVL SQLPAKQRKM VVVNPGRISS RAKAALDAAA KEMTKDKTKQ
660 670 680 690 700
QQKEALLVFF NRTAEAKIPC VVEYILDLLD SGREKFLVFA HHKVILDAVA
710 720 730 740 750
KELERKNVQH IRIDGSTPSA DREAQCQRFQ LSKGHTVALL SITAANMGLT
760 770 780 790 800
FSTADLVVFA ELFWNPGVLI QAEDRVHRIG QTNSVSIHYL VAKGTADDYL
810 820 830 840 850
WPLIQEKIKV LGEAGLSETN FSEMTEATDY VHKDPKQKTI YDLFQQSFED
860 870 880 890 900
DGNDMEFLEA AESFELGSTS GTSGNISQDL GDLLDEDEGS PPKKSRFEFF
910
DNWDSFSSPF
Length:910
Mass (Da):100,840
Last modified:March 1, 2003 - v1
Checksum:iF6B28F847BD6FC18
GO
Isoform 2 (identifier: Q8BJL0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     769-788: LIQAEDRVHRIGQTNSVSIH → GNVARVPLGMPRAEKYKIRK
     789-910: Missing.

Note: May be due to an intron retention.
Show »
Length:788
Mass (Da):87,126
Checksum:iAE70BAB0A799A3F8
GO
Isoform 3 (identifier: Q8BJL0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     508-574: DESHFLKNIK...HAFGLRYCDA → VSNGIALKYF...QPLSLCARLS
     575-910: Missing.

Note: No experimental confirmation available.
Show »
Length:574
Mass (Da):63,218
Checksum:i0E8930A229E115E8
GO
Isoform 4 (identifier: Q8BJL0-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     404-404: S → R
     405-910: Missing.

Note: No experimental confirmation available.
Show »
Length:404
Mass (Da):44,190
Checksum:i92E5249A242D75A6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti35A → T in AAH29078 (PubMed:15489334).Curated1
Sequence conflicti78F → L in AAH29078 (PubMed:15489334).Curated1
Sequence conflicti529K → N in BAE32320 (PubMed:16141072).Curated1
Sequence conflicti685K → R in BAC31469 (PubMed:16141072).Curated1
Sequence conflicti717 – 725Missing in AAG47648 (PubMed:10857751).Curated9
Sequence conflicti717T → R in AAF24985 (PubMed:10857751).Curated1
Sequence conflicti719 – 721SAD → TRA in AAF24985 (PubMed:10857751).Curated3
Sequence conflicti724 – 725AQ → LK in AAF24985 (PubMed:10857751).Curated2
Sequence conflicti743T → P in AAF24985 (PubMed:10857751).Curated1
Sequence conflicti834D → A in AAF24985 (PubMed:10857751).Curated1
Sequence conflicti834Missing in AAG47648 (PubMed:10857751).Curated1
Sequence conflicti866 – 868LGS → IKN in AAF24985 (PubMed:10857751).Curated3

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_036216404S → R in isoform 4. 1 Publication1
Alternative sequenceiVSP_036217405 – 910Missing in isoform 4. 1 PublicationAdd BLAST506
Alternative sequenceiVSP_036218508 – 574DESHF…RYCDA → VSNGIALKYFVCLDTRKGST DLGICVLLGPLWALRREGNR NRCLSFIENDFFIPFLKQPL SLCARLS in isoform 3. 1 PublicationAdd BLAST67
Alternative sequenceiVSP_036219575 – 910Missing in isoform 3. 1 PublicationAdd BLAST336
Alternative sequenceiVSP_012919769 – 788LIQAE…SVSIH → GNVARVPLGMPRAEKYKIRK in isoform 2. 1 PublicationAdd BLAST20
Alternative sequenceiVSP_012920789 – 910Missing in isoform 2. 1 PublicationAdd BLAST122

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF088884 mRNA Translation: AAF24985.1
AF209773 Genomic DNA Translation: AAG47648.1
AK042332 mRNA Translation: BAE20630.1
AK043129 mRNA Translation: BAC31469.1
AK077878 mRNA Translation: BAC37047.1
AK083488 mRNA Translation: BAC38933.1
AK154020 mRNA Translation: BAE32320.1
AK169465 mRNA Translation: BAE41189.1
BC029078 mRNA Translation: AAH29078.1
CCDSiCCDS35609.1 [Q8BJL0-1]
RefSeqiNP_061287.2, NM_018817.2 [Q8BJL0-1]
XP_006496204.1, XM_006496141.3 [Q8BJL0-1]
XP_006496205.1, XM_006496142.3 [Q8BJL0-1]
XP_006496206.1, XM_006496143.3 [Q8BJL0-1]
XP_006496207.1, XM_006496144.1 [Q8BJL0-1]
UniGeneiMm.274232

Genome annotation databases

EnsembliENSMUST00000047615; ENSMUSP00000047589; ENSMUSG00000039354 [Q8BJL0-1]
ENSMUST00000152225; ENSMUSP00000137833; ENSMUSG00000039354 [Q8BJL0-1]
GeneIDi54380
KEGGimmu:54380
UCSCiuc007bko.2 mouse [Q8BJL0-4]
uc007bkq.2 mouse [Q8BJL0-3]
uc007bkr.2 mouse [Q8BJL0-2]
uc007bks.2 mouse [Q8BJL0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSMAL1_MOUSE
AccessioniPrimary (citable) accession number: Q8BJL0
Secondary accession number(s): Q3TEQ9
, Q3U4W0, Q3V3A8, Q8BVK7, Q8BXW4, Q8K309, Q9EQK8, Q9QYC4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2003
Last modified: April 25, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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