UniProtKB - Q8BJL0 (SMAL1_MOUSE)
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1
Smarcal1
Functioni
ATP-dependent annealing helicase that binds selectively to fork DNA relative to ssDNA or dsDNA and catalyzes the rewinding of the stably unwound DNA. Rewinds single-stranded DNA bubbles that are stably bound by replication protein A (RPA). Acts throughout the genome to reanneal stably unwound DNA, performing the opposite reaction of many enzymes, such as helicases and polymerases, that unwind DNA. May play an important role in DNA damage response by acting at stalled replication forks (By similarity).
By similarityRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 417 – 424 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ATP-dependent activity, acting on DNA Source: MGI
- ATP-dependent chromatin remodeler activity Source: InterPro
- ATP-dependent DNA/DNA annealing activity Source: UniProtKB
- helicase activity Source: UniProtKB-KW
- hydrolase activity Source: UniProtKB-KW
GO - Biological processi
- cellular response to DNA damage stimulus Source: UniProtKB
- DNA repair Source: GO_Central
- double-strand break repair via nonhomologous end joining Source: MGI
- regulation of transcription by RNA polymerase II Source: UniProtKB
- replication fork processing Source: UniProtKB
- replication fork protection Source: GO_Central
Keywordsi
Molecular function | Helicase, Hydrolase |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1 (EC:3.6.4.-)Alternative name(s): HepA-related protein Short name: mharp Sucrose nonfermenting protein 2-like 1 |
Gene namesi | Name:Smarcal1 Synonyms:Harp |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1859183, Smarcal1 |
VEuPathDBi | HostDB:ENSMUSG00000039354 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Note: Recruited to damaged DNA regions.By similarity
Nucleus
- DNA replication factor A complex Source: Ensembl
- nuclear replication fork Source: GO_Central
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
Other locations
- site of double-strand break Source: UniProtKB
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000074349 | 2 – 910 | SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1Add BLAST | 909 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineBy similarity | 1 | |
Modified residuei | 118 | PhosphoserineBy similarity | 1 | |
Modified residuei | 165 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q8BJL0 |
MaxQBi | Q8BJL0 |
PaxDbi | Q8BJL0 |
PeptideAtlasi | Q8BJL0 |
PRIDEi | Q8BJL0 |
ProteomicsDBi | 261515 [Q8BJL0-1] 261516 [Q8BJL0-2] 261517 [Q8BJL0-3] 261518 [Q8BJL0-4] |
PTM databases
iPTMneti | Q8BJL0 |
PhosphoSitePlusi | Q8BJL0 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000039354, Expressed in oocyte and 289 other tissues |
Genevisiblei | Q8BJL0, MM |
Interactioni
Subunit structurei
Interacts with RPA2; the interaction is direct and mediates the recruitment by the RPA complex of SMARCAL1 to sites of DNA damage.
By similarityProtein-protein interaction databases
BioGRIDi | 207633, 7 interactors |
STRINGi | 10090.ENSMUSP00000047589 |
Miscellaneous databases
RNActi | Q8BJL0, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q8BJL0 |
SMRi | Q8BJL0 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 199 – 269 | HARP 1PROSITE-ProRule annotationAdd BLAST | 71 | |
Domaini | 286 – 357 | HARP 2PROSITE-ProRule annotationAdd BLAST | 72 | |
Domaini | 404 – 559 | Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 156 | |
Domaini | 674 – 827 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 154 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 198 | DisorderedSequence analysisAdd BLAST | 198 | |
Regioni | 2 – 29 | Mediates interaction with RPA2By similarityAdd BLAST | 28 | |
Regioni | 868 – 896 | DisorderedSequence analysisAdd BLAST | 29 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 3 – 34 | Sequence analysisAdd BLAST | 32 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 508 – 511 | DESH box | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 7 – 26 | Basic and acidic residuesSequence analysisAdd BLAST | 20 | |
Compositional biasi | 30 – 54 | Polar residuesSequence analysisAdd BLAST | 25 | |
Compositional biasi | 66 – 112 | Polar residuesSequence analysisAdd BLAST | 47 | |
Compositional biasi | 123 – 147 | Polar residuesSequence analysisAdd BLAST | 25 |
Sequence similaritiesi
Keywords - Domaini
Coiled coil, RepeatPhylogenomic databases
eggNOGi | KOG1000, Eukaryota |
GeneTreei | ENSGT00940000157608 |
HOGENOMi | CLU_000315_33_1_1 |
InParanoidi | Q8BJL0 |
OMAi | QEEMPTA |
OrthoDBi | 1082831at2759 |
PhylomeDBi | Q8BJL0 |
TreeFami | TF106474 |
Family and domain databases
Gene3Di | 3.40.50.10810, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR030101, HARP(SMARCAL1) IPR010003, HARP_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR038718, SNF2-like_sf IPR000330, SNF2_N |
PANTHERi | PTHR45766:SF3, PTHR45766:SF3, 2 hits |
Pfami | View protein in Pfam PF07443, HARP, 2 hits PF00271, Helicase_C, 1 hit PF00176, SNF2-rel_dom, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 2 hits |
PROSITEi | View protein in PROSITE PS51467, HARP, 2 hits PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSLPLTEEQR KKIEENRQKA LARRAEKLSE QPQSAASGSS AAGPSQSKQG
60 70 80 90 100
SLLNLLAEPS KPVGHASIFK QQNLSNSFPT DQRPHSSRCS QPSPAEETTG
110 120 130 140 150
LWKTQGEMST ACPKPNPSPP GASNQPLLGY KSSEGQPQAT WDTGASSSGP
160 170 180 190 200
FPRDPELEAK AARPSTSRQS ISDSFYVLGG KTPRTEGRPP NILQTTPQNT
210 220 230 240 250
GFLRGACIKT GDRFRVKIGY NQELIAVFKS LPSRHYDSFT KTWDFSMSDY
260 270 280 290 300
RALMKAVERL STVSLKPLDE AGGSVGGQTS LPSAPSLTFV TGKCMLISRV
310 320 330 340 350
RFEVDIGYSE AVIGLFKQME SRSYDIKTRK WSFLLEEHNK LIARSRELKQ
360 370 380 390 400
VQLDPLPKTV TLAFASQLEK TSPKLKADVP EADLSGVDAK LVSSLMPFQR
410 420 430 440 450
EGVSFAISKR GRLLLADDMG LGKTVQAICI AAFYRKEWPL LVVVPSSVRF
460 470 480 490 500
TWEQAFLRWL PSLSPENINV VVTGKGRLTA GLVNIVSFDL LCKLERQLKT
510 520 530 540 550
PFKVVIIDES HFLKNIKTAR CRAAVPILKV AKRVILLSGT PAMSRPAELY
560 570 580 590 600
TQIIAVKPTF FPQFHAFGLR YCDAKRLPWG WDYSGSSNLG ELKLLLEEAI
610 620 630 640 650
MLRRLKSDVL SQLPAKQRKM VVVNPGRISS RAKAALDAAA KEMTKDKTKQ
660 670 680 690 700
QQKEALLVFF NRTAEAKIPC VVEYILDLLD SGREKFLVFA HHKVILDAVA
710 720 730 740 750
KELERKNVQH IRIDGSTPSA DREAQCQRFQ LSKGHTVALL SITAANMGLT
760 770 780 790 800
FSTADLVVFA ELFWNPGVLI QAEDRVHRIG QTNSVSIHYL VAKGTADDYL
810 820 830 840 850
WPLIQEKIKV LGEAGLSETN FSEMTEATDY VHKDPKQKTI YDLFQQSFED
860 870 880 890 900
DGNDMEFLEA AESFELGSTS GTSGNISQDL GDLLDEDEGS PPKKSRFEFF
910
DNWDSFSSPF
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD3YUX5 | D3YUX5_MOUSE | SWI/SNF-related matrix-associated a... | Smarcal1 | 194 | Annotation score: | ||
D3Z0J8 | D3Z0J8_MOUSE | SWI/SNF-related matrix-associated a... | Smarcal1 | 98 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 35 | A → T in AAH29078 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 78 | F → L in AAH29078 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 529 | K → N in BAE32320 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 685 | K → R in BAC31469 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 717 – 725 | Missing in AAG47648 (PubMed:10857751).Curated | 9 | |
Sequence conflicti | 717 | T → R in AAF24985 (PubMed:10857751).Curated | 1 | |
Sequence conflicti | 719 – 721 | SAD → TRA in AAF24985 (PubMed:10857751).Curated | 3 | |
Sequence conflicti | 724 – 725 | AQ → LK in AAF24985 (PubMed:10857751).Curated | 2 | |
Sequence conflicti | 743 | T → P in AAF24985 (PubMed:10857751).Curated | 1 | |
Sequence conflicti | 834 | D → A in AAF24985 (PubMed:10857751).Curated | 1 | |
Sequence conflicti | 834 | Missing in AAG47648 (PubMed:10857751).Curated | 1 | |
Sequence conflicti | 866 – 868 | LGS → IKN in AAF24985 (PubMed:10857751).Curated | 3 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_036216 | 404 | S → R in isoform 4. 1 Publication | 1 | |
Alternative sequenceiVSP_036217 | 405 – 910 | Missing in isoform 4. 1 PublicationAdd BLAST | 506 | |
Alternative sequenceiVSP_036218 | 508 – 574 | DESHF…RYCDA → VSNGIALKYFVCLDTRKGST DLGICVLLGPLWALRREGNR NRCLSFIENDFFIPFLKQPL SLCARLS in isoform 3. 1 PublicationAdd BLAST | 67 | |
Alternative sequenceiVSP_036219 | 575 – 910 | Missing in isoform 3. 1 PublicationAdd BLAST | 336 | |
Alternative sequenceiVSP_012919 | 769 – 788 | LIQAE…SVSIH → GNVARVPLGMPRAEKYKIRK in isoform 2. 1 PublicationAdd BLAST | 20 | |
Alternative sequenceiVSP_012920 | 789 – 910 | Missing in isoform 2. 1 PublicationAdd BLAST | 122 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF088884 mRNA Translation: AAF24985.1 AF209773 Genomic DNA Translation: AAG47648.1 AK042332 mRNA Translation: BAE20630.1 AK043129 mRNA Translation: BAC31469.1 AK077878 mRNA Translation: BAC37047.1 AK083488 mRNA Translation: BAC38933.1 AK154020 mRNA Translation: BAE32320.1 AK169465 mRNA Translation: BAE41189.1 BC029078 mRNA Translation: AAH29078.1 |
CCDSi | CCDS35609.1 [Q8BJL0-1] |
RefSeqi | NP_061287.2, NM_018817.2 [Q8BJL0-1] XP_006496204.1, XM_006496141.3 [Q8BJL0-1] XP_006496205.1, XM_006496142.3 [Q8BJL0-1] XP_006496206.1, XM_006496143.3 [Q8BJL0-1] XP_006496207.1, XM_006496144.1 [Q8BJL0-1] |
Genome annotation databases
Ensembli | ENSMUST00000047615; ENSMUSP00000047589; ENSMUSG00000039354 [Q8BJL0-1] ENSMUST00000152225; ENSMUSP00000137833; ENSMUSG00000039354 [Q8BJL0-1] |
GeneIDi | 54380 |
KEGGi | mmu:54380 |
UCSCi | uc007bko.2, mouse [Q8BJL0-4] uc007bkq.2, mouse [Q8BJL0-3] uc007bkr.2, mouse [Q8BJL0-2] uc007bks.2, mouse [Q8BJL0-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF088884 mRNA Translation: AAF24985.1 AF209773 Genomic DNA Translation: AAG47648.1 AK042332 mRNA Translation: BAE20630.1 AK043129 mRNA Translation: BAC31469.1 AK077878 mRNA Translation: BAC37047.1 AK083488 mRNA Translation: BAC38933.1 AK154020 mRNA Translation: BAE32320.1 AK169465 mRNA Translation: BAE41189.1 BC029078 mRNA Translation: AAH29078.1 |
CCDSi | CCDS35609.1 [Q8BJL0-1] |
RefSeqi | NP_061287.2, NM_018817.2 [Q8BJL0-1] XP_006496204.1, XM_006496141.3 [Q8BJL0-1] XP_006496205.1, XM_006496142.3 [Q8BJL0-1] XP_006496206.1, XM_006496143.3 [Q8BJL0-1] XP_006496207.1, XM_006496144.1 [Q8BJL0-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4O66 | X-ray | 1.90 | A/B/C/D | 197-268 | [»] | |
AlphaFoldDBi | Q8BJL0 | |||||
SMRi | Q8BJL0 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 207633, 7 interactors |
STRINGi | 10090.ENSMUSP00000047589 |
PTM databases
iPTMneti | Q8BJL0 |
PhosphoSitePlusi | Q8BJL0 |
Proteomic databases
EPDi | Q8BJL0 |
MaxQBi | Q8BJL0 |
PaxDbi | Q8BJL0 |
PeptideAtlasi | Q8BJL0 |
PRIDEi | Q8BJL0 |
ProteomicsDBi | 261515 [Q8BJL0-1] 261516 [Q8BJL0-2] 261517 [Q8BJL0-3] 261518 [Q8BJL0-4] |
Protocols and materials databases
Antibodypediai | 34237, 193 antibodies from 31 providers |
DNASUi | 54380 |
Genome annotation databases
Ensembli | ENSMUST00000047615; ENSMUSP00000047589; ENSMUSG00000039354 [Q8BJL0-1] ENSMUST00000152225; ENSMUSP00000137833; ENSMUSG00000039354 [Q8BJL0-1] |
GeneIDi | 54380 |
KEGGi | mmu:54380 |
UCSCi | uc007bko.2, mouse [Q8BJL0-4] uc007bkq.2, mouse [Q8BJL0-3] uc007bkr.2, mouse [Q8BJL0-2] uc007bks.2, mouse [Q8BJL0-1] |
Organism-specific databases
CTDi | 50485 |
MGIi | MGI:1859183, Smarcal1 |
VEuPathDBi | HostDB:ENSMUSG00000039354 |
Phylogenomic databases
eggNOGi | KOG1000, Eukaryota |
GeneTreei | ENSGT00940000157608 |
HOGENOMi | CLU_000315_33_1_1 |
InParanoidi | Q8BJL0 |
OMAi | QEEMPTA |
OrthoDBi | 1082831at2759 |
PhylomeDBi | Q8BJL0 |
TreeFami | TF106474 |
Miscellaneous databases
BioGRID-ORCSi | 54380, 3 hits in 74 CRISPR screens |
ChiTaRSi | Smarcal1, mouse |
PROi | PR:Q8BJL0 |
RNActi | Q8BJL0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000039354, Expressed in oocyte and 289 other tissues |
Genevisiblei | Q8BJL0, MM |
Family and domain databases
Gene3Di | 3.40.50.10810, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR030101, HARP(SMARCAL1) IPR010003, HARP_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR038718, SNF2-like_sf IPR000330, SNF2_N |
PANTHERi | PTHR45766:SF3, PTHR45766:SF3, 2 hits |
Pfami | View protein in Pfam PF07443, HARP, 2 hits PF00271, Helicase_C, 1 hit PF00176, SNF2-rel_dom, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 2 hits |
PROSITEi | View protein in PROSITE PS51467, HARP, 2 hits PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SMAL1_MOUSE | |
Accessioni | Q8BJL0Primary (citable) accession number: Q8BJL0 Secondary accession number(s): Q3TEQ9 Q9QYC4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 2005 |
Last sequence update: | March 1, 2003 | |
Last modified: | May 25, 2022 | |
This is version 147 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families