Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Tyrosyl-DNA phosphodiesterase 1

Gene

Tdp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. Hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. Acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. Has low 3'exonuclease activity and can remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. Has no exonuclease activity towards DNA or RNA with a 3'phosphate (By similarity).By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei264NucleophileBy similarity1
Binding sitei266SubstrateBy similarity1
Active sitei494Proton donor/acceptorBy similarity1
Binding sitei496SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • DNA repair Source: MGI
  • double-strand break repair Source: MGI
  • single strand break repair Source: MGI

Keywordsi

Molecular functionExonuclease, Hydrolase, Nuclease
Biological processDNA damage, DNA repair

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosyl-DNA phosphodiesterase 1 (EC:3.1.4.-By similarity)
Short name:
Tyr-DNA phosphodiesterase 1
Alternative name(s):
Protein expressed in male leptotene and zygotene spermatocytes 501
Short name:
MLZ-501
Gene namesi
Name:Tdp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1920036 Tdp1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice display an inability to rapidly repair single-strand DNA breaks due to covalent bonds between TOP1 and DNA. They are hypersentitive to camptothecin, topotecan and bleomycin.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002124871 – 609Tyrosyl-DNA phosphodiesterase 1Add BLAST609

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei61PhosphoserineBy similarity1
Modified residuei119PhosphoserineCombined sources1
Modified residuei132PhosphoserineBy similarity1
Modified residuei148PhosphothreonineBy similarity1
Modified residuei149PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8BJ37
MaxQBiQ8BJ37
PaxDbiQ8BJ37
PRIDEiQ8BJ37

PTM databases

iPTMnetiQ8BJ37
PhosphoSitePlusiQ8BJ37

Expressioni

Tissue specificityi

Ubiquitous.2 Publications

Gene expression databases

CleanExiMM_TDP1

Interactioni

Subunit structurei

Monomer.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei519Interaction with DNABy similarity1

Protein-protein interaction databases

IntActiQ8BJ37, 1 interactor
STRINGi10090.ENSMUSP00000021594

Structurei

3D structure databases

ProteinModelPortaliQ8BJ37
SMRiQ8BJ37
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni401 – 404Interaction with DNABy similarity4

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2031 Eukaryota
ENOG410XQPZ LUCA
HOGENOMiHOG000067775
HOVERGENiHBG061242
InParanoidiQ8BJ37

Family and domain databases

Gene3Di3.30.870.20, 1 hit
InterProiView protein in InterPro
IPR010347 Tdp1
IPR027415 TDP_C
PANTHERiPTHR12415 PTHR12415, 1 hit
PfamiView protein in Pfam
PF06087 Tyr-DNA_phospho, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q8BJ37-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSQESSYGKW TISSSDESED EKPKPDKPSA SSHPQAGQGV SKELIYTCSE
60 70 80 90 100
ARKVAHKRQI SPVKFNDADS VLPHKKQKSD SPEGLGWCLS SSDDDQQPDV
110 120 130 140 150
TQQEQPKRVL PQEKKHVSSP DVTTAQKVVD RSPPASLRPQ RADDEYETSG
160 170 180 190 200
EGQDIWDMLD KGNPFQFYLT RVSGIKAKYN SKALHIKDIL SPLFGTLVSS
210 220 230 240 250
AQFNYCFDVD WLIKQYPPEF RKNPILLVHG DKREAKADLH AQAKPYANIS
260 270 280 290 300
LCQAKLDIAF GTHHTKMMLL LYEEGLRVVI HTSNLIREDW HQKTQGIWLS
310 320 330 340 350
PLYPRIDQGS HTAGESSTRF KADLTSYLTA YNAPPLQEWI DIIQEHDLSE
360 370 380 390 400
TNVYLIGSTP GRFQGSHRDN WGHFRLRKLL QAHAPSTPKG ECWPIVGQFS
410 420 430 440 450
SIGSLGPDES KWLCSEFKDS LLALREEGRP PGKSAVPLHL IYPSVENVRT
460 470 480 490 500
SLEGYPAGGS LPYSIQTAEK QRWLHSYFHK WSAETSGRSN AMPHIKTYMR
510 520 530 540 550
PSPDFSKLAW FLVTSANLSK AAWGALEKNG TQLMIRSYEL GVLFLPSAFG
560 570 580 590 600
LDTFKVKQKF FSSSCEPTAS FPVPYDLPPE LYRSKDRPWI WNIPYVKAPD

THGNMWVPS
Length:609
Mass (Da):68,689
Last modified:October 24, 2003 - v2
Checksum:iE23C49C9993E1FC9
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B8JJC1B8JJC1_MOUSE
Tyrosyl-DNA phosphodiesterase 1
Tdp1 mCG_14730
609Annotation score:
D3Z075D3Z075_MOUSE
Tyrosyl-DNA phosphodiesterase 1
Tdp1
51Annotation score:

Sequence cautioni

The sequence AAH19804 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti223N → K (PubMed:15489334).Curated1
Sequence conflicti312T → A in AAH19804 (PubMed:15489334).Curated1
Sequence conflicti325T → I in AAH19804 (PubMed:15489334).Curated1
Sequence conflicti550 – 609GLDTF…MWVPS → VSNIVPARSLTCVVNGTLFS SSWLSVGLAW in BAC28500 (PubMed:16141072).CuratedAdd BLAST60

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK033868 mRNA Translation: BAC28500.1
BC019804 mRNA Translation: AAH19804.1 Different initiation.
CCDSiCCDS26105.2
UniGeneiMm.312323
Mm.487464
Mm.488658

Genome annotation databases

UCSCiuc007osf.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK033868 mRNA Translation: BAC28500.1
BC019804 mRNA Translation: AAH19804.1 Different initiation.
CCDSiCCDS26105.2
UniGeneiMm.312323
Mm.487464
Mm.488658

3D structure databases

ProteinModelPortaliQ8BJ37
SMRiQ8BJ37
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BJ37, 1 interactor
STRINGi10090.ENSMUSP00000021594

PTM databases

iPTMnetiQ8BJ37
PhosphoSitePlusiQ8BJ37

Proteomic databases

EPDiQ8BJ37
MaxQBiQ8BJ37
PaxDbiQ8BJ37
PRIDEiQ8BJ37

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiuc007osf.2 mouse

Organism-specific databases

MGIiMGI:1920036 Tdp1

Phylogenomic databases

eggNOGiKOG2031 Eukaryota
ENOG410XQPZ LUCA
HOGENOMiHOG000067775
HOVERGENiHBG061242
InParanoidiQ8BJ37

Miscellaneous databases

ChiTaRSiTdp1 mouse
PROiPR:Q8BJ37
SOURCEiSearch...

Gene expression databases

CleanExiMM_TDP1

Family and domain databases

Gene3Di3.30.870.20, 1 hit
InterProiView protein in InterPro
IPR010347 Tdp1
IPR027415 TDP_C
PANTHERiPTHR12415 PTHR12415, 1 hit
PfamiView protein in Pfam
PF06087 Tyr-DNA_phospho, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTYDP1_MOUSE
AccessioniPrimary (citable) accession number: Q8BJ37
Secondary accession number(s): Q8VE39
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 24, 2003
Last sequence update: October 24, 2003
Last modified: May 10, 2017
This is version 112 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again