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Protein

Exopolyphosphatase PRUNE1

Gene

Prune1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Phosphodiesterase (PDE) that has higher activity toward cAMP than cGMP, as substrate. Plays a role in cell proliferation, migration and differentiation, and acts as a negative regulator of NME1. Plays a role in the regulation of neurogenesis. Involved in the regulation of microtubule polymerization.By similarity

Catalytic activityi

Diphosphate + H2O = 2 phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Enzyme regulationi

Activated by magnesium ions and inhibited by manganese ions. Inhibited by dipyridamole, moderately sensitive to IBMX and inhibited by vinpocetine (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi28Manganese 1By similarity1
Metal bindingi30Manganese 2By similarity1
Metal bindingi106Manganese 1By similarity1
Metal bindingi106Manganese 2By similarity1
Metal bindingi179Manganese 2By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandManganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Exopolyphosphatase PRUNE1 (EC:3.6.1.1)
Alternative name(s):
PRUNEM1
Gene namesi
Name:Prune1
Synonyms:Prune
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1925152 Prune1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003379881 – 454Exopolyphosphatase PRUNE1Add BLAST454

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei400PhosphoserineCombined sources1
Modified residuei411PhosphothreonineCombined sources1
Modified residuei415PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8BIW1
MaxQBiQ8BIW1
PaxDbiQ8BIW1
PeptideAtlasiQ8BIW1
PRIDEiQ8BIW1

PTM databases

iPTMnetiQ8BIW1
PhosphoSitePlusiQ8BIW1

Expressioni

Developmental stagei

In the developing embryo, a high level of expression is confined to the nervous system particularly in the dorsal root ganglia, cranial nerves, and neural retina. From E10.5, expressed at low levels in the basal plate along the entire neural tube, while at E12.5 the expression in the nervous system is definitively stronger, especially in the cranial and dorsal root ganglia and in the spinal nerves. In the hypothalamus, the expression is confined to the retro-chiasmatic area (RCH) and is also detectable in the remnant of the Rathke's pouch. In the developing eye, exclusively expressed in the prospective neural retina, equally distributed in both the deep and superficial layers. At E16.5, expression is still detectable in the outer neuroblast layer of the neural retina.1 Publication

Gene expression databases

BgeeiENSMUSG00000015711
GenevisibleiQ8BIW1 MM

Interactioni

Subunit structurei

Homooligomer. Able to homodimerize via its C-terminal domain. Interacts with NME1. Interacts with GSK3; at focal adhesion complexes where paxillin and vinculin are colocalized. Interacts with alpha and beta tubulin.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi230866, 4 interactors
IntActiQ8BIW1, 2 interactors
STRINGi10090.ENSMUSP00000015855

Structurei

3D structure databases

ProteinModelPortaliQ8BIW1
SMRiQ8BIW1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni394 – 421Essential for homodimerizationBy similarityAdd BLAST28

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi106 – 108DHH motifBy similarity3

Sequence similaritiesi

Belongs to the PPase class C family. Prune subfamily.Curated

Phylogenomic databases

eggNOGiKOG4129 Eukaryota
COG1227 LUCA
GeneTreeiENSGT00450000040262
HOGENOMiHOG000115724
HOVERGENiHBG058142
InParanoidiQ8BIW1
KOiK01514
OMAiIYMDLEA
OrthoDBiEOG091G0E88
PhylomeDBiQ8BIW1
TreeFamiTF323914

Family and domain databases

Gene3Di3.10.310.20, 1 hit
InterProiView protein in InterPro
IPR001667 DDH_dom
IPR038763 DHH_sf
IPR004097 DHHA2
IPR038222 DHHA2_dom_sf
PfamiView protein in Pfam
PF01368 DHH, 1 hit
PF02833 DHHA2, 1 hit
SMARTiView protein in SMART
SM01131 DHHA2, 1 hit
SUPFAMiSSF64182 SSF64182, 1 hit

Sequencei

Sequence statusi: Complete.

Q8BIW1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDYLQDCRA ALQDSRPLHV VLGNEACDLD SMVSALALAF YLTKTSEAED
60 70 80 90 100
IFIPVLNIKR SELPLRGDNV FFLQEVKIPE PALIFRDEID LLALHQAGQL
110 120 130 140 150
TLILVDHHIL PKSDAALEEA VAEVLDHRPI EQKYCPPCHV SVELVGSCAT
160 170 180 190 200
LVTERILQGA PETLDRQTAA LLHGTIILDC VNMDTNIGKA TPKDSKYVEE
210 220 230 240 250
LEALFPDLPK RKDIFDSLQK AKFDVSGLTT EQMLRKDQKT VYRQGTKVAI
260 270 280 290 300
SAIYMDLKAF LQRTDLFTDL SSFCHDHSYD ALVAMTIFFN TQNEPVRQLA
310 320 330 340 350
IFCPHEALRM TICGILERST SPPLKLTPIP STSPNLQAYH QGNTQVSRKK
360 370 380 390 400
LLPVLQEALS AYLDSAKMAS GQSEVAVGMS REQVDKDLDK ASNSLISGLS
410 420 430 440 450
QDEEDPPLPP TPMNSLVDEC PLDQGLPKFS AEAVFEKCSQ ISLSQSARAC

TSNK
Length:454
Mass (Da):50,239
Last modified:March 1, 2003 - v1
Checksum:i646FBF6B345028E6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti75E → A in AAC95291 (PubMed:10602478).Curated1
Sequence conflicti397 – 398SG → LW in AAC95291 (PubMed:10602478).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF051908 mRNA Translation: AAC95291.2
AK081637 mRNA Translation: BAC38278.1
AK142160 mRNA Translation: BAE24953.1
BC057546 mRNA Translation: AAH57546.1
BC058635 mRNA Translation: AAH58635.1
CCDSiCCDS17610.1
RefSeqiNP_775482.1, NM_173347.2
UniGeneiMm.14155
Mm.486494

Genome annotation databases

EnsembliENSMUST00000015855; ENSMUSP00000015855; ENSMUSG00000015711
GeneIDi229589
KEGGimmu:229589
UCSCiuc008qiz.1 mouse

Similar proteinsi

Entry informationi

Entry nameiPRUN1_MOUSE
AccessioniPrimary (citable) accession number: Q8BIW1
Secondary accession number(s): Q80VU0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: March 1, 2003
Last modified: May 23, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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