Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein Hook homolog 1

Gene

Hook1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for spermatid differentiation. Probably involved in the positioning of the microtubules of the manchette and the flagellum in relation to the membrane skeleton (PubMed:12075009). Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex) (By similarity).By similarity1 Publication

GO - Molecular functioni

  • actin binding Source: MGI
  • dynein light intermediate chain binding Source: GO_Central
  • identical protein binding Source: MGI
  • microtubule binding Source: MGI

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein
Biological processDifferentiation, Protein transport, Spermatogenesis, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Hook homolog 1
Short name:
mHK1
Gene namesi
Name:Hook1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1925213 Hook1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Involvement in diseasei

Defects in Hook1 are the cause of the azh (abnormal spermatozoon head shape) mutant phenotype, which induces spermatozoa with highly abnormal head morphology that differs drastically from the compact and hook-shaped head of the normal sperm, leading to a strong decrease of fertility.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002191931 – 728Protein Hook homolog 1Add BLAST728

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei235PhosphoserineBy similarity1
Modified residuei719PhosphoserineBy similarity1
Modified residuei727PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8BIL5
PaxDbiQ8BIL5
PeptideAtlasiQ8BIL5
PRIDEiQ8BIL5

PTM databases

iPTMnetiQ8BIL5
PhosphoSitePlusiQ8BIL5

Expressioni

Tissue specificityi

Mainly expressed in testis.1 Publication

Gene expression databases

BgeeiENSMUSG00000028572 Expressed in 232 organ(s), highest expression level in testis
CleanExiMM_HOOK1
ExpressionAtlasiQ8BIL5 baseline and differential
GenevisibleiQ8BIL5 MM

Interactioni

Subunit structurei

Self-associates (By similarity). Component of the FTS/Hook/FHIP complex (FHF complex), composed of AKTIP/FTS, FAM160A2, and one or more members of the Hook family of proteins HOOK1, HOOK2, and HOOK3 (By similarity). May interact directly with AKTIP/FTS, HOOK2 and HOOK3 (By similarity). Associates with several subunits of the homotypic vesicular sorting complex (the HOPS complex) including VPS16, VPS18, VPS39 and VPS41; these interactions may be indirect (By similarity). Interacts with CCDC181 (PubMed:28283191). Interacts (via coiled-coil region) with RIMBP3 (via C-terminus) (PubMed:19091768). Interacts with LRGUK (via guanylate kinase-like domain) (PubMed:28003339). Interacts with microtubules (By similarity).By similarity3 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi219052, 4 interactors
IntActiQ8BIL5, 2 interactors
STRINGi10090.ENSMUSP00000030306

Structurei

Secondary structure

1728
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ8BIL5
SMRiQ8BIL5
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8BIL5

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 128Calponin-homology (CH)PROSITE-ProRule annotationAdd BLAST117

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 555Sufficient for interaction with microtubulesBy similarityAdd BLAST555
Regioni657 – 728Sufficient for interaction with AKTIP and VPS18By similarityAdd BLAST72

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili168 – 443Sequence analysisAdd BLAST276
Coiled coili477 – 658Sequence analysisAdd BLAST182

Sequence similaritiesi

Belongs to the hook family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IG1M Eukaryota
ENOG410XQ3G LUCA
GeneTreeiENSGT00690000101702
HOGENOMiHOG000294112
HOVERGENiHBG051920
InParanoidiQ8BIL5
KOiK16612
OMAiNTMVAKK
OrthoDBiEOG091G06O9
PhylomeDBiQ8BIL5
TreeFamiTF320231

Family and domain databases

Gene3Di1.10.418.10, 1 hit
InterProiView protein in InterPro
IPR001715 CH-domain
IPR036872 CH_dom_sf
IPR008636 Hook-like_fam
PfamiView protein in Pfam
PF05622 HOOK, 1 hit
PROSITEiView protein in PROSITE
PS50021 CH, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8BIL5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEDPQPLPQS ELPLCDSLII WLQTFKTASP CQDVKQLTNG VTMAQVLHQI
60 70 80 90 100
DVAWFSESWL SRIKDDVGDN WRIKASNLKK VLHGITSYYH EFLGQQISEE
110 120 130 140 150
LIPDLNQITE CADPVELGRL LQLILGCAVN CEKKQEHIKN IMTLEESVQH
160 170 180 190 200
VVMTAIQELM SKEIVISPAS DTVGELEQQL KRALEELQEA IAEKEELKQR
210 220 230 240 250
CQELDMQVTT LQDEKNSLVS ENEMMNEKLD QLDGSFDDPN TMVAKKYFHV
260 270 280 290 300
QLQLEQLQEE NYRLEAAKDD YRVHCEELEK QLIEFQHRND ELTSLAEETR
310 320 330 340 350
ALKDEIDVLR ATSDKANKLE STVEVYRQKL QDLNDLRKQV KSLQETNMMY
360 370 380 390 400
MHNTVSLEEE LKKANAARAQ LETYKRQVQD LHTKLSSESK RADTLAFEMK
410 420 430 440 450
RLEEKHETLL KEKERLIEQR DTLKETNEEL RCSKAQQDHL NQADASATKS
460 470 480 490 500
YENLAAEIMP VEYREVFIRL QHENKMLRLQ QEGTENERIE QLQEQLEQKH
510 520 530 540 550
RKMNELETEQ RLSKERIGEL QQQIEDLQKS LQEQGSKSEG ESSSKLKQKL
560 570 580 590 600
EAHMEKLTEV HEELQKKQEL IEDLQPDISQ NAQKISELEA ALQKKDEDMK
610 620 630 640 650
AMEERYKMYL EKARNVIKTL DPKLNPASAE IMLLRKQLAE KERRIEILES
660 670 680 690 700
ECKVAKLRDY EEKLIVSAWY NKSLAFQKLG MESRLVSGAS ACKDSVAAAP
710 720
ARSFLAQQRH ITNTRRNLSV KVPAAASD
Length:728
Mass (Da):84,439
Last modified:February 2, 2004 - v2
Checksum:i5AC9A80D5D61581C
GO
Isoform 2 (identifier: Q8BIL5-2) [UniParc]FASTAAdd to basket
Also known as: sv

The sequence of this isoform differs from the canonical sequence as follows:
     543-549: SSKLKQK → VSNRVHN
     550-728: Missing.

Show »
Length:549
Mass (Da):64,011
Checksum:i506AE75949F9B521
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q8BJ23Q8BJ23_MOUSE
Protein Hook homolog 1
Hook1
221Annotation score:

Sequence cautioni

The sequence AAH30877 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti185E → Q in BAC31686 (PubMed:16141072).Curated1
Sequence conflicti334N → T in BAC33977 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_009340543 – 549SSKLKQK → VSNRVHN in isoform 2. 2 Publications7
Alternative sequenceiVSP_009341550 – 728Missing in isoform 2. 2 PublicationsAdd BLAST179

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF487912 mRNA Translation: AAM74055.1
AK020924 mRNA Translation: BAB32257.1
AK043870 mRNA Translation: BAC31686.1
AK049897 mRNA Translation: BAC33977.1
BC030877 mRNA Translation: AAH30877.1 Different initiation.
BC061688 mRNA Translation: AAH61688.1
CCDSiCCDS18367.1 [Q8BIL5-1]
RefSeqiNP_084290.1, NM_030014.2 [Q8BIL5-1]
UniGeneiMm.49994

Genome annotation databases

EnsembliENSMUST00000030306; ENSMUSP00000030306; ENSMUSG00000028572 [Q8BIL5-1]
GeneIDi77963
KEGGimmu:77963
UCSCiuc008ttb.1 mouse [Q8BIL5-2]
uc008ttc.1 mouse [Q8BIL5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF487912 mRNA Translation: AAM74055.1
AK020924 mRNA Translation: BAB32257.1
AK043870 mRNA Translation: BAC31686.1
AK049897 mRNA Translation: BAC33977.1
BC030877 mRNA Translation: AAH30877.1 Different initiation.
BC061688 mRNA Translation: AAH61688.1
CCDSiCCDS18367.1 [Q8BIL5-1]
RefSeqiNP_084290.1, NM_030014.2 [Q8BIL5-1]
UniGeneiMm.49994

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WIXNMR-A12-162[»]
ProteinModelPortaliQ8BIL5
SMRiQ8BIL5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi219052, 4 interactors
IntActiQ8BIL5, 2 interactors
STRINGi10090.ENSMUSP00000030306

PTM databases

iPTMnetiQ8BIL5
PhosphoSitePlusiQ8BIL5

Proteomic databases

EPDiQ8BIL5
PaxDbiQ8BIL5
PeptideAtlasiQ8BIL5
PRIDEiQ8BIL5

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030306; ENSMUSP00000030306; ENSMUSG00000028572 [Q8BIL5-1]
GeneIDi77963
KEGGimmu:77963
UCSCiuc008ttb.1 mouse [Q8BIL5-2]
uc008ttc.1 mouse [Q8BIL5-1]

Organism-specific databases

CTDi51361
MGIiMGI:1925213 Hook1

Phylogenomic databases

eggNOGiENOG410IG1M Eukaryota
ENOG410XQ3G LUCA
GeneTreeiENSGT00690000101702
HOGENOMiHOG000294112
HOVERGENiHBG051920
InParanoidiQ8BIL5
KOiK16612
OMAiNTMVAKK
OrthoDBiEOG091G06O9
PhylomeDBiQ8BIL5
TreeFamiTF320231

Miscellaneous databases

EvolutionaryTraceiQ8BIL5
PROiPR:Q8BIL5
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028572 Expressed in 232 organ(s), highest expression level in testis
CleanExiMM_HOOK1
ExpressionAtlasiQ8BIL5 baseline and differential
GenevisibleiQ8BIL5 MM

Family and domain databases

Gene3Di1.10.418.10, 1 hit
InterProiView protein in InterPro
IPR001715 CH-domain
IPR036872 CH_dom_sf
IPR008636 Hook-like_fam
PfamiView protein in Pfam
PF05622 HOOK, 1 hit
PROSITEiView protein in PROSITE
PS50021 CH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHOOK1_MOUSE
AccessioniPrimary (citable) accession number: Q8BIL5
Secondary accession number(s): Q8BIZ2
, Q8K0P1, Q8K454, Q9CTN6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: February 2, 2004
Last modified: September 12, 2018
This is version 137 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again