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Protein

RUN and FYVE domain-containing protein 1

Gene

Rufy1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in early endosomal trafficking.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri646 – 704FYVE-typePROSITE-ProRule annotationAdd BLAST59

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processEndocytosis, Protein transport, Transport
LigandLipid-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-1660499 Synthesis of PIPs at the plasma membrane

Names & Taxonomyi

Protein namesi
Recommended name:
RUN and FYVE domain-containing protein 1
Alternative name(s):
Rab4-interacting protein
Gene namesi
Name:Rufy1
Synonyms:Rabip4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2429762 Rufy1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002458291 – 712RUN and FYVE domain-containing protein 1Add BLAST712

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei393PhosphotyrosineBy similarity1
Modified residuei404PhosphotyrosineBy similarity1
Modified residuei624PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation on Tyr-393 and/or Tyr-404 is required for interaction with BMX and endosomal targeting.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8BIJ7
MaxQBiQ8BIJ7
PaxDbiQ8BIJ7
PeptideAtlasiQ8BIJ7
PRIDEiQ8BIJ7

PTM databases

iPTMnetiQ8BIJ7
PhosphoSitePlusiQ8BIJ7
SwissPalmiQ8BIJ7

Expressioni

Tissue specificityi

Broadly expressed.1 Publication

Gene expression databases

BgeeiENSMUSG00000020375 Expressed in 280 organ(s), highest expression level in primary oocyte
CleanExiMM_RUFY1
GenevisibleiQ8BIJ7 MM

Interactioni

Subunit structurei

Interacts with BMX. May interact with SSB (By similarity). Interacts with RAB4 and RAB5 that have been activated by GTP-binding.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ8BIJ7, 1 interactor
STRINGi10090.ENSMUSP00000020643

Structurei

3D structure databases

ProteinModelPortaliQ8BIJ7
SMRiQ8BIJ7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini143 – 275RUNPROSITE-ProRule annotationAdd BLAST133

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni619 – 629Interaction with RAB41 PublicationAdd BLAST11

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili325 – 378Sequence analysisAdd BLAST54
Coiled coili409 – 621Sequence analysisAdd BLAST213

Domaini

The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns3P-enriched membranes is substantially increased in acidic conditions (By similarity).By similarity

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri646 – 704FYVE-typePROSITE-ProRule annotationAdd BLAST59

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG1729 Eukaryota
KOG4381 Eukaryota
ENOG410XRAX LUCA
GeneTreeiENSGT00550000074558
HOGENOMiHOG000030913
HOVERGENiHBG057053
InParanoidiQ8BIJ7
KOiK12482
OMAiLDSEYPP
OrthoDBiEOG091G042J
PhylomeDBiQ8BIJ7
TreeFamiTF323904

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR004012 Run_dom
IPR037213 Run_dom_sf
IPR000306 Znf_FYVE
IPR017455 Znf_FYVE-rel
IPR011011 Znf_FYVE_PHD
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF01363 FYVE, 1 hit
PF02759 RUN, 1 hit
SMARTiView protein in SMART
SM00064 FYVE, 1 hit
SM00593 RUN, 1 hit
SUPFAMiSSF140741 SSF140741, 1 hit
SSF57903 SSF57903, 1 hit
PROSITEiView protein in PROSITE
PS50826 RUN, 1 hit
PS50178 ZF_FYVE, 1 hit

Sequencei

Sequence statusi: Complete.

Q8BIJ7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAARGECRRA GQDSEPEPER EPESEPGPEP EPQAGLESGE AFEIVDRSQL
60 70 80 90 100
PSPGELRSAS RPRVADSWSA PILTLARRAT GNLSASCGSA LRAAAGLGDG
110 120 130 140 150
GGGGERAASK GQMMEERANL MHMMKLSIKV LLQSALSLGR SLDADYAPLQ
160 170 180 190 200
QFFVVMEHCL KHGLKVKKSF IGQNKSFFGP LELVEKLCPE ASDIATSVRN
210 220 230 240 250
LPELKTAVGR GRAWLYLALM QKKLADYLKV LIDNKQLLSE FYEPEALMME
260 270 280 290 300
EEGMVIVGLL VGLNVLDANL CLKGEDLDSQ VGVIDFSLCL KDAQDLDSGR
310 320 330 340 350
EHERITDVLD QKNYVEELNR HLSCTVGDLQ TKIDGLEKTN SKLQEELSAA
360 370 380 390 400
TDRICSLQKE QQQLREQNEV IRERSEKSVE ITKQDTKVEL ETYKQTRQGL
410 420 430 440 450
DEMYSDVWKQ LKEEKKVRLE LEKELELQIG MKTEMEIAMK LLEKDTHEKQ
460 470 480 490 500
DTLVALRQQL EEVKAINLQM FHKVQSAESS LQQKNEAIAS FEGKTTQVMS
510 520 530 540 550
SMKQMEERLQ QAERARQAAE ERSHKLQQEL SGRGSALQLQ LSQLRDQCSG
560 570 580 590 600
LEKELKSEKE QRQALQRELQ REKDTSCLLQ TELQQVEGLK KELRELQDEK
610 620 630 640 650
AELRKVCEEQ EQALQEMGLH LSQSKLKMED IKEVNKALKG HTWLKDDEAT
660 670 680 690 700
HCKQCEKDFS ISRRKHHCRN CGHIFCNTCS SNELALPSYP KPVRVCDSCH
710
TLLLQRCSST AS
Length:712
Mass (Da):80,376
Last modified:March 1, 2003 - v1
Checksum:i71416057ADB71222
GO

Sequence cautioni

The sequence CAC17732 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti493G → R in CAC17732 (PubMed:11172003).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK046625 mRNA Translation: BAC32811.2
AK046633 mRNA Translation: BAC32815.1
AK051178 mRNA Translation: BAC34548.1
AL645904 Genomic DNA No translation available.
AJ250024 mRNA Translation: CAC17732.1 Different initiation.
CCDSiCCDS24635.1
RefSeqiNP_766145.1, NM_172557.2
UniGeneiMm.390400

Genome annotation databases

EnsembliENSMUST00000020643; ENSMUSP00000020643; ENSMUSG00000020375
GeneIDi216724
KEGGimmu:216724
UCSCiuc007isn.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK046625 mRNA Translation: BAC32811.2
AK046633 mRNA Translation: BAC32815.1
AK051178 mRNA Translation: BAC34548.1
AL645904 Genomic DNA No translation available.
AJ250024 mRNA Translation: CAC17732.1 Different initiation.
CCDSiCCDS24635.1
RefSeqiNP_766145.1, NM_172557.2
UniGeneiMm.390400

3D structure databases

ProteinModelPortaliQ8BIJ7
SMRiQ8BIJ7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BIJ7, 1 interactor
STRINGi10090.ENSMUSP00000020643

PTM databases

iPTMnetiQ8BIJ7
PhosphoSitePlusiQ8BIJ7
SwissPalmiQ8BIJ7

Proteomic databases

EPDiQ8BIJ7
MaxQBiQ8BIJ7
PaxDbiQ8BIJ7
PeptideAtlasiQ8BIJ7
PRIDEiQ8BIJ7

Protocols and materials databases

DNASUi216724
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020643; ENSMUSP00000020643; ENSMUSG00000020375
GeneIDi216724
KEGGimmu:216724
UCSCiuc007isn.1 mouse

Organism-specific databases

CTDi80230
MGIiMGI:2429762 Rufy1

Phylogenomic databases

eggNOGiKOG1729 Eukaryota
KOG4381 Eukaryota
ENOG410XRAX LUCA
GeneTreeiENSGT00550000074558
HOGENOMiHOG000030913
HOVERGENiHBG057053
InParanoidiQ8BIJ7
KOiK12482
OMAiLDSEYPP
OrthoDBiEOG091G042J
PhylomeDBiQ8BIJ7
TreeFamiTF323904

Enzyme and pathway databases

ReactomeiR-MMU-1660499 Synthesis of PIPs at the plasma membrane

Miscellaneous databases

ChiTaRSiRufy1 mouse
PROiPR:Q8BIJ7
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020375 Expressed in 280 organ(s), highest expression level in primary oocyte
CleanExiMM_RUFY1
GenevisibleiQ8BIJ7 MM

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR004012 Run_dom
IPR037213 Run_dom_sf
IPR000306 Znf_FYVE
IPR017455 Znf_FYVE-rel
IPR011011 Znf_FYVE_PHD
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF01363 FYVE, 1 hit
PF02759 RUN, 1 hit
SMARTiView protein in SMART
SM00064 FYVE, 1 hit
SM00593 RUN, 1 hit
SUPFAMiSSF140741 SSF140741, 1 hit
SSF57903 SSF57903, 1 hit
PROSITEiView protein in PROSITE
PS50826 RUN, 1 hit
PS50178 ZF_FYVE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRUFY1_MOUSE
AccessioniPrimary (citable) accession number: Q8BIJ7
Secondary accession number(s): Q8BKQ4, Q8BL21, Q9EPM6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: March 1, 2003
Last modified: November 7, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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