UniProtKB - Q8BIF2 (RFOX3_MOUSE)
Protein
RNA binding protein fox-1 homolog 3
Gene
Rbfox3
Organism
Mus musculus (Mouse)
Status
Functioni
Pre-mRNA alternative splicing regulator. Regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD).2 Publications
Caution
Initial characterization was derived from usage of a monoclonal antibody (A60) directed to an unknown protein called NeuN (PubMed:15605376 and PubMed:1483388), but later identified as RBFOX3.1 Publication
GO - Molecular functioni
- DNA binding Source: MGI
- mRNA binding Source: GO_Central
GO - Biological processi
- mRNA processing Source: UniProtKB-KW
- nervous system development Source: GO_Central
- regulation of alternative mRNA splicing, via spliceosome Source: MGI
- RNA splicing Source: UniProtKB-KW
Keywordsi
Molecular function | RNA-binding |
Biological process | mRNA processing, mRNA splicing |
Names & Taxonomyi
Protein namesi | Recommended name: RNA binding protein fox-1 homolog 3Alternative name(s): Fox-1 homolog C Hexaribonucleotide-binding protein 3 Short name: Fox-3 Neuronal nuclei antigen Short name: NeuN antigen |
Gene namesi | Name:Rbfox3 Synonyms:D11Bwg0517e, Hrnbp3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:106368, Rbfox3 |
Subcellular locationi
Nucleus
Other locations
Nucleus
Nucleus
- nucleus Source: MGI
Other locations
- cytoplasm Source: MGI
- neuronal cell body Source: MGI
- perikaryon Source: MGI
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
Knockout mice have significantly reduced brain weight, impaired neurofilament expression and decreased white matter volume, but normal total body mass. They show increased susceptibility to seizures and reduced anxiety-related behaviors compared with wild type littermates, as well as defective hippocampal gene expression and deficits in synaptic transmission and plasticity in the dentate gyrus.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000349208 | 1 – 374 | RNA binding protein fox-1 homolog 3Add BLAST | 374 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 223 | Asymmetric dimethylarginine; alternateCombined sources | 1 | |
Modified residuei | 223 | Omega-N-methylarginine; alternateCombined sources | 1 | |
Modified residuei | 319 | Asymmetric dimethylarginineCombined sources | 1 |
Post-translational modificationi
Phosphorylated.1 Publication
Keywords - PTMi
MethylationProteomic databases
PaxDbi | Q8BIF2 |
PeptideAtlasi | Q8BIF2 |
PRIDEi | Q8BIF2 |
PTM databases
iPTMneti | Q8BIF2 |
PhosphoSitePlusi | Q8BIF2 |
Expressioni
Tissue specificityi
Widely expressed in brain, including in cerebral cortex, hippocampus, thalamus, caudate/putamen, cerebellum, as well as in the spinal cord (at protein level). Not expressed in all neuronal cells within a region, in cerebellum, expression is absent in Purkinje cells (at protein level). Expressed in the retina in the ganglion cells and some cells in the inner nuclear layer, but absent from the photoreceptor cells and most cells in the inner nuclear layer (at protein level).4 Publications
Developmental stagei
In the neural tube, expressed as early as 9.5 dpc and expression is confined to the nervous system. By 12.5 dpc, can be found in the developing ventral horns and is also detected in the developing dorsal horns as well as in the dorsal root ganglion. Not detected in the ventricular zone, roof plate, floor plate or marginal zone (developing white matter). It is expressed from embryonic stage to adulthood.1 Publication
Inductioni
By retinoic acid. Expression is up-regulated in P19 cells during neural differentiation upon retinoic acid treatment (at the protein level).2 Publications
Gene expression databases
Bgeei | ENSMUSG00000025576, Expressed in lumbar subsegment of spinal cord and 157 other tissues |
ExpressionAtlasi | Q8BIF2, baseline and differential |
Genevisiblei | Q8BIF2, MM |
Interactioni
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 100 | Interaction with RNABy similarity | 1 | |
Sitei | 108 | Interaction with RNABy similarity | 1 | |
Sitei | 109 | Interaction with RNABy similarity | 1 | |
Sitei | 133 | Interaction with RNABy similarity | 1 | |
Sitei | 138 | Interaction with RNABy similarity | 1 | |
Sitei | 142 | Interaction with RNABy similarity | 1 | |
Sitei | 166 | Interaction with RNABy similarity | 1 | |
Sitei | 176 | Interaction with RNABy similarity | 1 |
Binary interactionsi
Hide detailsQ8BIF2
With | #Exp. | IntAct |
---|---|---|
Hif1a [Q61221] | 2 | EBI-4567146,EBI-298954 |
HIF1A [Q16665] from Homo sapiens. | 2 | EBI-4567146,EBI-447269 |
Protein-protein interaction databases
BioGRIDi | 206871, 1 interactor |
IntActi | Q8BIF2, 4 interactors |
MINTi | Q8BIF2 |
STRINGi | 10090.ENSMUSP00000017576 |
Miscellaneous databases
RNActi | Q8BIF2, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 99 – 175 | RRMPROSITE-ProRule annotationAdd BLAST | 77 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 4 – 58 | Pro-richAdd BLAST | 55 | |
Compositional biasi | 242 – 295 | Pro-richAdd BLAST | 54 |
Phylogenomic databases
eggNOGi | KOG0125, Eukaryota |
GeneTreei | ENSGT00940000159924 |
HOGENOMi | CLU_048440_0_0_1 |
InParanoidi | Q8BIF2 |
OMAi | MLAWHPA |
OrthoDBi | 871288at2759 |
PhylomeDBi | Q8BIF2 |
TreeFami | TF315942 |
Family and domain databases
CDDi | cd12407, RRM_FOX1_like, 1 hit |
Gene3Di | 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR025670, Fox-1_C_dom IPR034237, FOX1_RRM IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR017325, RNA-bd_Fox-1 IPR000504, RRM_dom |
Pfami | View protein in Pfam PF12414, Fox-1_C, 1 hit PF00076, RRM_1, 1 hit |
PIRSFi | PIRSF037932, Ataxin_2_bd_A2BP, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit |
s (5+)i Sequence
Sequence statusi: Complete.
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 5 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8BIF2-1) [UniParc]FASTAAdd to basket
Also known as: Fox3v1, Fox-3-L
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAQPYPPAQY PPPPQNGIPA EYAPPPPHPT QDYSGQTPVP PEHGMTLYTP
60 70 80 90 100
AQTHPEQPGT EASTQPIAGT QTVPQADEAA QTDNQQLHPS DPTEKQQPKR
110 120 130 140 150
LHVSNIPFRF RDPDLRQMFG QFGKILDVEI IFNERGSKGF GFVTFETSSD
160 170 180 190 200
ADRAREKLNG TIVEGRKIEV NNATARVMTN KKPGNPYANG WKLNPVVGTV
210 220 230 240 250
YGPEFYAVTS FPYPTTGTAV AYRGAHLRGR GRAVYNTFRA APPPPPIPTY
260 270 280 290 300
GAALEQTLVK MPVPWAGLAP CPLPPQQTPE PAYPTSPAFP PLSCPFASRV
310 320 330 340 350
VYQDGFYGAE IYGGYAAYRY AQPAAATAAA YSDSYGRVYA AADPYHHTIG
360 370
PTATYSIGTM ASLCRGGYSR FTPY
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA2A4W9 | A2A4W9_MOUSE | RNA-binding protein fox-1 homolog 3 | Rbfox3 | 169 | Annotation score: | ||
B7ZC11 | B7ZC11_MOUSE | RNA-binding protein fox-1 homolog 3 | Rbfox3 | 145 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 266 | A → V in BAC37531 (PubMed:19713214).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_035219 | 139 – 169 | Missing in isoform 3. 1 PublicationAdd BLAST | 31 | |
Alternative sequenceiVSP_043949 | 253 – 299 | Missing in isoform 4 and isoform 5. 1 PublicationAdd BLAST | 47 | |
Alternative sequenceiVSP_035220 | 361 – 374 | Missing in isoform 2, isoform 3 and isoform 4. 2 PublicationsAdd BLAST | 14 |
Sequence databases
Genome annotation databases
Ensembli | ENSMUST00000017576; ENSMUSP00000017576; ENSMUSG00000025576 [Q8BIF2-1] ENSMUST00000069343; ENSMUSP00000069598; ENSMUSG00000025576 [Q8BIF2-3] ENSMUST00000103023; ENSMUSP00000099312; ENSMUSG00000025576 [Q8BIF2-4] ENSMUST00000106278; ENSMUSP00000101885; ENSMUSG00000025576 [Q8BIF2-4] ENSMUST00000117731; ENSMUSP00000113636; ENSMUSG00000025576 [Q8BIF2-2] ENSMUST00000120061; ENSMUSP00000113987; ENSMUSG00000025576 [Q8BIF2-5] |
GeneIDi | 52897 |
KEGGi | mmu:52897 |
UCSCi | uc007mpl.1, mouse [Q8BIF2-4] uc007mpm.1, mouse [Q8BIF2-5] uc007mpn.1, mouse [Q8BIF2-1] uc007mpp.1, mouse [Q8BIF2-3] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
3D structure databases
SMRi | Q8BIF2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 206871, 1 interactor |
IntActi | Q8BIF2, 4 interactors |
MINTi | Q8BIF2 |
STRINGi | 10090.ENSMUSP00000017576 |
PTM databases
iPTMneti | Q8BIF2 |
PhosphoSitePlusi | Q8BIF2 |
Proteomic databases
PaxDbi | Q8BIF2 |
PeptideAtlasi | Q8BIF2 |
PRIDEi | Q8BIF2 |
Protocols and materials databases
Antibodypediai | 32645, 418 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000017576; ENSMUSP00000017576; ENSMUSG00000025576 [Q8BIF2-1] ENSMUST00000069343; ENSMUSP00000069598; ENSMUSG00000025576 [Q8BIF2-3] ENSMUST00000103023; ENSMUSP00000099312; ENSMUSG00000025576 [Q8BIF2-4] ENSMUST00000106278; ENSMUSP00000101885; ENSMUSG00000025576 [Q8BIF2-4] ENSMUST00000117731; ENSMUSP00000113636; ENSMUSG00000025576 [Q8BIF2-2] ENSMUST00000120061; ENSMUSP00000113987; ENSMUSG00000025576 [Q8BIF2-5] |
GeneIDi | 52897 |
KEGGi | mmu:52897 |
UCSCi | uc007mpl.1, mouse [Q8BIF2-4] uc007mpm.1, mouse [Q8BIF2-5] uc007mpn.1, mouse [Q8BIF2-1] uc007mpp.1, mouse [Q8BIF2-3] |
Organism-specific databases
CTDi | 146713 |
MGIi | MGI:106368, Rbfox3 |
Phylogenomic databases
eggNOGi | KOG0125, Eukaryota |
GeneTreei | ENSGT00940000159924 |
HOGENOMi | CLU_048440_0_0_1 |
InParanoidi | Q8BIF2 |
OMAi | MLAWHPA |
OrthoDBi | 871288at2759 |
PhylomeDBi | Q8BIF2 |
TreeFami | TF315942 |
Miscellaneous databases
BioGRID-ORCSi | 52897, 1 hit in 17 CRISPR screens |
ChiTaRSi | Rbfox3, mouse |
PROi | PR:Q8BIF2 |
RNActi | Q8BIF2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000025576, Expressed in lumbar subsegment of spinal cord and 157 other tissues |
ExpressionAtlasi | Q8BIF2, baseline and differential |
Genevisiblei | Q8BIF2, MM |
Family and domain databases
CDDi | cd12407, RRM_FOX1_like, 1 hit |
Gene3Di | 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR025670, Fox-1_C_dom IPR034237, FOX1_RRM IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR017325, RNA-bd_Fox-1 IPR000504, RRM_dom |
Pfami | View protein in Pfam PF12414, Fox-1_C, 1 hit PF00076, RRM_1, 1 hit |
PIRSFi | PIRSF037932, Ataxin_2_bd_A2BP, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RFOX3_MOUSE | |
Accessioni | Q8BIF2Primary (citable) accession number: Q8BIF2 Secondary accession number(s): A2A4W7 Q7TQI4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 2, 2008 |
Last sequence update: | September 2, 2008 | |
Last modified: | December 2, 2020 | |
This is version 133 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot