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Protein

F-box/WD repeat-containing protein 8

Gene

Fbxw8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Substrate-recognition component of a Cul7-RING ubiquitin-protein ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. The Cul7-RING(FBXW8) complex mediates ubiquitination and consequent degradation of GORASP1, acting as a component of the ubiquitin ligase pathway that regulates Golgi morphogenesis and dendrite patterning in brain. The Cul7-RING(FBXW8) complex also mediates ubiquitination of MAP4K1/HPK1: recognizes and binds autophosphorylated MAP4K1/HPK1, leading to its degradation, thereby affecting cell proliferation and differentiation. Associated component of the 3M complex, suggesting that it mediates some of 3M complex functions (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-8951664 Neddylation
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
F-box/WD repeat-containing protein 8
Alternative name(s):
F-box and WD-40 domain-containing protein 8
Gene namesi
Name:Fbxw8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1923041 Fbxw8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus

Pathology & Biotechi

Disruption phenotypei

Reduced embryo size and neonatal lethality. Embryos and placentas are smaller and only a third of the expected number of mice survived birth. Mice that survive remain smaller throughout postnatal development.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000509981 – 598F-box/WD repeat-containing protein 8Add BLAST598

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei84PhosphoserineCombined sources1
Modified residuei86PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ8BIA4
PeptideAtlasiQ8BIA4
PRIDEiQ8BIA4

PTM databases

iPTMnetiQ8BIA4
PhosphoSitePlusiQ8BIA4

Expressioni

Tissue specificityi

Widely expressed. Expressed at higher level in skeletal muscle, cartilage and lung.1 Publication

Gene expression databases

BgeeiENSMUSG00000032867 Expressed in 270 organ(s), highest expression level in ear vesicle
CleanExiMM_FBXW8
GenevisibleiQ8BIA4 MM

Interactioni

Subunit structurei

Part of a SCF-like complex consisting of CUL7, RBX1, SKP1, FBXW8 and GLMN. Interacts with OBSL1, CUL1, CUL2, SKP1, CCT6B, PFDN5, CCT2, CCT3, CCT6A, CCT7, VBP1, CCDC8, ARF1, TRIP13, PDCD5 and GORASP1. Interacts with MAP4K1/HPK1 (when autophosphorylated). Associated component of the 3M complex (By similarity). Interacts with CUL7. Interacts with POUF51 (when phosphorylated on 'Ser-347') (PubMed:29153991).By similarity2 Publications

Protein-protein interaction databases

BioGridi231155, 7 interactors
CORUMiQ8BIA4
STRINGi10090.ENSMUSP00000047012

Structurei

3D structure databases

ProteinModelPortaliQ8BIA4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini113 – 159F-boxPROSITE-ProRule annotationAdd BLAST47
Repeati197 – 245WD 1Add BLAST49
Repeati253 – 294WD 2Add BLAST42
Repeati297 – 336WD 3Add BLAST40
Repeati430 – 470WD 4Add BLAST41
Repeati473 – 510WD 5Add BLAST38

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiENOG410IQD1 Eukaryota
ENOG4110ZIH LUCA
GeneTreeiENSGT00390000017221
HOGENOMiHOG000112555
HOVERGENiHBG051597
InParanoidiQ8BIA4
KOiK10264
OMAiFEHDARI
OrthoDBiEOG091G0KZ9
PhylomeDBiQ8BIA4
TreeFamiTF332593

Family and domain databases

Gene3Di2.130.10.10, 2 hits
InterProiView protein in InterPro
IPR036047 F-box-like_dom_sf
IPR001810 F-box_dom
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF12937 F-box-like, 1 hit
SMARTiView protein in SMART
SM00256 FBOX, 1 hit
SM00320 WD40, 5 hits
SUPFAMiSSF50978 SSF50978, 1 hit
SSF81383 SSF81383, 1 hit
PROSITEiView protein in PROSITE
PS50181 FBOX, 1 hit
PS00678 WD_REPEATS_1, 1 hit
PS50082 WD_REPEATS_2, 1 hit
PS50294 WD_REPEATS_REGION, 2 hits

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q8BIA4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDDHNLEEFR RHWQEELAQS QALRRRRRLE AGERRSPRRP EAGARGEPAS
60 70 80 90 100
GYLGLAQGLL EGAGRPPAPR PGRGGDRKDT SSRSRSPPDR DATEPEPLVD
110 120 130 140 150
QLIRDLNELD DVPFFDVRLP YELAINIFQY LNRRELGLCA QVSKTWKVIA
160 170 180 190 200
EDEVLWYRLC RQEGHLPHSR FSDYTCWKLI LQECLAKEHT LRANWKNRKG
210 220 230 240 250
AVSELEHVPD AVLCDVRSHD GVVIAGYTSG DVRVWDTRTW DYVAPFLESE
260 270 280 290 300
SEEEDPGMQP YVSFVRINSS LAVAAYEDGI LNIWDLRTGR FPIFRFEHDA
310 320 330 340 350
RIQALALSQE KPIVATASAF DVVMLYPNEE GHWHVASEFE VQKLVDYLEI
360 370 380 390 400
VPNTGRYPVA IATAGDLVYL LKADDSARTL HYVYGQPATC LDVSASQVAF
410 420 430 440 450
GVKSLGWVYE GNKILVYSLE AERCLSKLGN ALGDFTCVNI RDSPPNLMVS
460 470 480 490 500
GNMDRRVRIH DLRSDKIALS LSAHQLGVSA VQMDDWKVVS GGEEGLVSVW
510 520 530 540 550
DYRMNQKLWE VHSRHPVRYL SFNSHSLITA NVPYEKVLRN SDLDNFACHR
560 570 580 590
RHRGLIHAYE FAVDQLAFQS PLPVCRLPRD IMAGYSYDLA LSFPHDSI
Length:598
Mass (Da):67,956
Last modified:October 3, 2003 - v2
Checksum:i4FEAF1C4384D3CA5
GO
Isoform 2 (identifier: Q8BIA4-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     457-598: Missing.

Show »
Length:456
Mass (Da):51,673
Checksum:i2C90E47D7E946821
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti67P → T in BAC28712 (PubMed:16141072).Curated1
Sequence conflicti171F → S in AAH09095 (PubMed:15489334).Curated1
Sequence conflicti247 – 248LE → WS in BAC33128 (PubMed:16141072).Curated2
Sequence conflicti280I → V in BAC35527 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_008502457 – 598Missing in isoform 2. 1 PublicationAdd BLAST142

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK034447 mRNA Translation: BAC28712.1
AK047695 mRNA Translation: BAC33128.1
AK047756 mRNA Translation: BAC33147.1
AK053794 mRNA Translation: BAC35527.1
BC009095 mRNA Translation: AAH09095.2
BC024091 mRNA Translation: AAH24091.1
CCDSiCCDS19609.1 [Q8BIA4-1]
RefSeqiNP_766309.2, NM_172721.2 [Q8BIA4-1]
UniGeneiMm.221769

Genome annotation databases

EnsembliENSMUST00000049474; ENSMUSP00000047012; ENSMUSG00000032867 [Q8BIA4-1]
GeneIDi231672
KEGGimmu:231672
UCSCiuc008zge.1 mouse [Q8BIA4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK034447 mRNA Translation: BAC28712.1
AK047695 mRNA Translation: BAC33128.1
AK047756 mRNA Translation: BAC33147.1
AK053794 mRNA Translation: BAC35527.1
BC009095 mRNA Translation: AAH09095.2
BC024091 mRNA Translation: AAH24091.1
CCDSiCCDS19609.1 [Q8BIA4-1]
RefSeqiNP_766309.2, NM_172721.2 [Q8BIA4-1]
UniGeneiMm.221769

3D structure databases

ProteinModelPortaliQ8BIA4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi231155, 7 interactors
CORUMiQ8BIA4
STRINGi10090.ENSMUSP00000047012

PTM databases

iPTMnetiQ8BIA4
PhosphoSitePlusiQ8BIA4

Proteomic databases

PaxDbiQ8BIA4
PeptideAtlasiQ8BIA4
PRIDEiQ8BIA4

Protocols and materials databases

DNASUi231672
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000049474; ENSMUSP00000047012; ENSMUSG00000032867 [Q8BIA4-1]
GeneIDi231672
KEGGimmu:231672
UCSCiuc008zge.1 mouse [Q8BIA4-1]

Organism-specific databases

CTDi26259
MGIiMGI:1923041 Fbxw8

Phylogenomic databases

eggNOGiENOG410IQD1 Eukaryota
ENOG4110ZIH LUCA
GeneTreeiENSGT00390000017221
HOGENOMiHOG000112555
HOVERGENiHBG051597
InParanoidiQ8BIA4
KOiK10264
OMAiFEHDARI
OrthoDBiEOG091G0KZ9
PhylomeDBiQ8BIA4
TreeFamiTF332593

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-MMU-8951664 Neddylation
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

PROiPR:Q8BIA4
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032867 Expressed in 270 organ(s), highest expression level in ear vesicle
CleanExiMM_FBXW8
GenevisibleiQ8BIA4 MM

Family and domain databases

Gene3Di2.130.10.10, 2 hits
InterProiView protein in InterPro
IPR036047 F-box-like_dom_sf
IPR001810 F-box_dom
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF12937 F-box-like, 1 hit
SMARTiView protein in SMART
SM00256 FBOX, 1 hit
SM00320 WD40, 5 hits
SUPFAMiSSF50978 SSF50978, 1 hit
SSF81383 SSF81383, 1 hit
PROSITEiView protein in PROSITE
PS50181 FBOX, 1 hit
PS00678 WD_REPEATS_1, 1 hit
PS50082 WD_REPEATS_2, 1 hit
PS50294 WD_REPEATS_REGION, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiFBXW8_MOUSE
AccessioniPrimary (citable) accession number: Q8BIA4
Secondary accession number(s): Q8BI62
, Q8BI75, Q8BI76, Q8CID8, Q921Z1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: October 3, 2003
Last modified: November 7, 2018
This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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