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Protein

Cell migration-inducing and hyaluronan-binding protein

Gene

Cemip

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates depolymerization of hyaluronic acid (HA) via the cell membrane-associated clathrin-coated pit endocytic pathway. Binds to hyaluronic acid. Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product, a process that may occur through rapid vesicle endocytosis and recycling without intracytoplasmic accumulation or digestion in lysosomes. Involved in hyaluronan catabolism in the dermis of the skin and arthritic synovium. Positively regulates epithelial-mesenchymal transition (EMT), and hence tumor cell growth, invasion and cancer dissemination. In collaboration with HSPA5/BIP, promotes cancer cell migration in a calcium and PKC-dependent manner. May be involved in hearing.1 Publication

Catalytic activityi

Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.1 Publication

Activity regulationi

Activity is up-regulated by histamine.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
LigandHyaluronic acid

Enzyme and pathway databases

ReactomeiR-MMU-2142850 Hyaluronan biosynthesis and export

Names & Taxonomyi

Protein namesi
Recommended name:
Cell migration-inducing and hyaluronan-binding protein (EC:3.2.1.351 Publication)
Alternative name(s):
Protein 12H19.01.T7
Gene namesi
Name:Cemip
Synonyms:Kiaa11991 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2443629 Cemip

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Coated pit, Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30By similarityAdd BLAST30
ChainiPRO_000002153931 – 1361Cell migration-inducing and hyaluronan-binding proteinAdd BLAST1331

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi119N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi165N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi312N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi420N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi889N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi921N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

N-glycosylated; glycosylation is not necessary for HA-binding.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ8BI06
PRIDEiQ8BI06

PTM databases

iPTMnetiQ8BI06
PhosphoSitePlusiQ8BI06

Expressioni

Tissue specificityi

Expressed in Deiters' cells and various supporting cells in the organ of Corti including inner phalangeal, border, inner and outer pillar cells (at protein level). Weakly expressed in brain and testis. In ear, it is specifically expressed in inner ear. Expressed in Deiters' cells in the organ of Corti at P0 (postnatal day zero) before the onset of hearing, but disappears by day P7. Also expressed in fibrocytes of the spiral ligament and the spiral limbus through to P21, when the cochlea matures.3 Publications

Developmental stagei

Expressed throughout development.1 Publication

Gene expression databases

BgeeiENSMUSG00000052353 Expressed in 130 organ(s), highest expression level in vault of skull
CleanExiMM_9930013L23RIK
GenevisibleiQ8BI06 MM

Interactioni

Subunit structurei

Interacts with EPHA2 and ITPR3. Interacts with HSPA5/BIP; the interaction induces calcium leakage from the endoplasmic reticulum and cell migration. Interacts with clathrin heavy chain/CLTC (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ8BI06, 1 interactor
STRINGi10090.ENSMUSP00000063277

Structurei

3D structure databases

ProteinModelPortaliQ8BI06
SMRiQ8BI06
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini44 – 166G8PROSITE-ProRule annotationAdd BLAST123
Repeati572 – 594PbH1 1Add BLAST23
Repeati595 – 617PbH1 2Add BLAST23
Repeati719 – 741PbH1 3Add BLAST23
Repeati798 – 819PbH1 4Add BLAST22

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni295 – 591Necessary for its endoplasmic reticulum (ER) retention and interaction with HSPA5By similarityAdd BLAST297

Domaini

The signal sequence is essential in mediating its proper translocation, hyaluronic acid (HA) degradation activity and secretion.By similarity

Sequence similaritiesi

Belongs to the CEMIP family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IFCM Eukaryota
ENOG410XQ01 LUCA
GeneTreeiENSGT00780000121902
HOGENOMiHOG000290723
HOVERGENiHBG052198
InParanoidiQ8BI06
KOiK19031
OMAiHFTAYKN
OrthoDBiEOG091G00JT
TreeFamiTF316575

Family and domain databases

CDDicd13938 PANDER_like_TMEM2, 1 hit
InterProiView protein in InterPro
IPR019316 G8_domain
IPR039477 ILEI/PANDER_dom
IPR011050 Pectin_lyase_fold/virulence
IPR039473 TMEM2_PANDER-like
IPR025155 WxxW_domain
PfamiView protein in Pfam
PF10162 G8, 1 hit
PF15711 ILEI, 2 hits
PF13330 Mucin2_WxxW, 1 hit
SMARTiView protein in SMART
SM01225 G8, 1 hit
SUPFAMiSSF51126 SSF51126, 2 hits
PROSITEiView protein in PROSITE
PS51484 G8, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q8BI06-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MRASGRHDVS LKIVLATGCL LLANFSGASS AVATECPDQS PELQPWSPGH
60 70 80 90 100
NRDYQVHIGH GRKLLLTSSA TVHSITISGG GKLVIKDHHE HIVLRTRYIL
110 120 130 140 150
IDDGGELHAG SALCPFEGNF SIVLYGRADE NILPDPYYGL KYIGVDKGGT
160 170 180 190 200
LELHGQKKLS WTFLNKTLHP GGMQEGGYFF ERSWGHRGVI VHVIDAKLGT
210 220 230 240 250
VVHSDRFDTY RSKKESERLV QYLNAVPDGR ILSVAVNDEG SRNLDDTARK
260 270 280 290 300
AMTKLGSKHF LHLGFRHPWS FITVKGNPSS SVEDHIEYHG HKGSAAARVF
310 320 330 340 350
KLFQTEHGEH FNVSSSSEWV QDVEWTEWFD HDKVPQSKGG EKISDLRAAY
360 370 380 390 400
PGKICNRPID IQATTMDGVA LSTEVVYKNG QDYRFACYTR GRACRSYRVR
410 420 430 440 450
FLCGKPVRPK LTVSIDTNVN STILSLVDNV RSWRPGDTLV VASTDYSMYQ
460 470 480 490 500
AEEFRVLPCK ACTSTQVKVA GKPQYLHIGE EIDGVDMRAE VGLLTRNIVV
510 520 530 540 550
MGEMEDRCYP YTNHICDFFD FDTFGGHIKF ALGFKAAHLE GVELKYMGQQ
560 570 580 590 600
LVGQYPIHFH LAGDLDEQGG YDPPTYIRDL SIHHTFSRCI TVHGSNGLLI
610 620 630 640 650
KDVVGYNSLG HCFFTEDGPE ERNTFDHCLG LLVKSGTLLP SDRDSRMCKV
660 670 680 690 700
ITEDSYPGYI PKPRQDCNAV STFWMANPNN NLINCAAAGS EETGFWFIFH
710 720 730 740 750
HVPTGPSVGM YSPGYSEHIP LGKFYNNRAH SNYRAGMIID NGVKTTEASA
760 770 780 790 800
KDKRPFLSII SARYSPHQDA DPLKPREPAI IRHFTAYKNQ DHGAWLRGGD
810 820 830 840 850
VWLDSCRFAD NGIGLTLASG GTFPYDDGSK QEIKNSLFVG ESGNVGTEMM
860 870 880 890 900
DNRIWGPGGL DHSGRTLPIG QNFPIRGIQF YDGPINIQNC TFRKFAALEG
910 920 930 940 950
RHTSALAFRL NNAWQSCPHN NVTNIAFEDV PITSRVFFGE PGPWFNQLDM
960 970 980 990 1000
DGDKTSVFHD LDGSVSEYPG SYLTKDDNWL VRHPDCINVP DWRGAICSGR
1010 1020 1030 1040 1050
YAQMYIQAYK SSNLRMKIIK NDFPSHPLYL EGALTRSTHY QQYQPVITLQ
1060 1070 1080 1090 1100
KGYTIHWDQT APAELAIWLI NFNKGDWIRV GLCYPRGTTF SILSDVHNRL
1110 1120 1130 1140 1150
LKQTSKTGTF VRTLQMDKVE QSYPGRSHYY WDEDSGLLFL KLKAQNEREK
1160 1170 1180 1190 1200
FAFCSMKGCE RIKIKALLPR NAGISDCTAT AYPRFTERAI VDVPMPRKLF
1210 1220 1230 1240 1250
GAQLKTKDHF LEVKMESSRQ HFFHLRNDFA YIEVDGRRYP CSEDGIQIVV
1260 1270 1280 1290 1300
IDGSRGHVVS HGSFRNAILQ GIPWQLFNYV AAIPDNSIVL MASKGRYITR
1310 1320 1330 1340 1350
GPWTRVLEKL GADKGLKLKE KMAFVGFKGS FRPIWVTLET EDHKAKIFQV
1360
VPIPVVRKKK L
Length:1,361
Mass (Da):153,034
Last modified:July 27, 2011 - v4
Checksum:iACAFA439574B9E8C
GO
Isoform 2 (identifier: Q8BI06-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1155: Missing.

Note: No experimental confirmation available.
Show »
Length:206
Mass (Da):23,455
Checksum:iC0681FA02CA871EB
GO
Isoform 3 (identifier: Q8BI06-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1137-1361: Missing.

Note: No experimental confirmation available.
Show »
Length:1,136
Mass (Da):127,329
Checksum:i67B792155F5802D2
GO

Sequence cautioni

The sequence AAH56981 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC29586 differs from that shown. Reason: Frameshift at position 1136.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti197K → E in BAC29586 (PubMed:16141072).Curated1
Sequence conflicti590I → V in BAD02452 (PubMed:14577002).Curated1
Sequence conflicti662K → N in BAC29586 (PubMed:16141072).Curated1
Sequence conflicti880F → L in BAC29586 (PubMed:16141072).Curated1
Sequence conflicti1268 – 1270ILQ → PEF in AAG41062 (PubMed:11247670).Curated3

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0098161 – 1155Missing in isoform 2. 1 PublicationAdd BLAST1155
Alternative sequenceiVSP_0098171137 – 1361Missing in isoform 3. 1 PublicationAdd BLAST225

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY007814 mRNA Translation: AAG41061.1
AY007815 mRNA Translation: AAG41062.1
AB103331 mRNA Translation: BAD02452.1
AK036809 mRNA Translation: BAC29586.1 Sequence problems.
AC101659 Genomic DNA No translation available.
AC154141 Genomic DNA No translation available.
BC056981 mRNA Translation: AAH56981.1 Different initiation.
CCDSiCCDS21416.1 [Q8BI06-1]
RefSeqiNP_109653.3, NM_030728.4 [Q8BI06-1]
XP_006508416.1, XM_006508353.3 [Q8BI06-1]
XP_006508417.1, XM_006508354.3 [Q8BI06-1]
XP_017167893.1, XM_017312404.1 [Q8BI06-1]
UniGeneiMm.160389

Genome annotation databases

EnsembliENSMUST00000064174; ENSMUSP00000063277; ENSMUSG00000052353 [Q8BI06-1]
GeneIDi80982
KEGGimmu:80982
UCSCiuc009idy.3 mouse [Q8BI06-2]
uc009idz.2 mouse [Q8BI06-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY007814 mRNA Translation: AAG41061.1
AY007815 mRNA Translation: AAG41062.1
AB103331 mRNA Translation: BAD02452.1
AK036809 mRNA Translation: BAC29586.1 Sequence problems.
AC101659 Genomic DNA No translation available.
AC154141 Genomic DNA No translation available.
BC056981 mRNA Translation: AAH56981.1 Different initiation.
CCDSiCCDS21416.1 [Q8BI06-1]
RefSeqiNP_109653.3, NM_030728.4 [Q8BI06-1]
XP_006508416.1, XM_006508353.3 [Q8BI06-1]
XP_006508417.1, XM_006508354.3 [Q8BI06-1]
XP_017167893.1, XM_017312404.1 [Q8BI06-1]
UniGeneiMm.160389

3D structure databases

ProteinModelPortaliQ8BI06
SMRiQ8BI06
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BI06, 1 interactor
STRINGi10090.ENSMUSP00000063277

PTM databases

iPTMnetiQ8BI06
PhosphoSitePlusiQ8BI06

Proteomic databases

PaxDbiQ8BI06
PRIDEiQ8BI06

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000064174; ENSMUSP00000063277; ENSMUSG00000052353 [Q8BI06-1]
GeneIDi80982
KEGGimmu:80982
UCSCiuc009idy.3 mouse [Q8BI06-2]
uc009idz.2 mouse [Q8BI06-1]

Organism-specific databases

CTDi57214
MGIiMGI:2443629 Cemip
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410IFCM Eukaryota
ENOG410XQ01 LUCA
GeneTreeiENSGT00780000121902
HOGENOMiHOG000290723
HOVERGENiHBG052198
InParanoidiQ8BI06
KOiK19031
OMAiHFTAYKN
OrthoDBiEOG091G00JT
TreeFamiTF316575

Enzyme and pathway databases

ReactomeiR-MMU-2142850 Hyaluronan biosynthesis and export

Miscellaneous databases

PROiPR:Q8BI06
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000052353 Expressed in 130 organ(s), highest expression level in vault of skull
CleanExiMM_9930013L23RIK
GenevisibleiQ8BI06 MM

Family and domain databases

CDDicd13938 PANDER_like_TMEM2, 1 hit
InterProiView protein in InterPro
IPR019316 G8_domain
IPR039477 ILEI/PANDER_dom
IPR011050 Pectin_lyase_fold/virulence
IPR039473 TMEM2_PANDER-like
IPR025155 WxxW_domain
PfamiView protein in Pfam
PF10162 G8, 1 hit
PF15711 ILEI, 2 hits
PF13330 Mucin2_WxxW, 1 hit
SMARTiView protein in SMART
SM01225 G8, 1 hit
SUPFAMiSSF51126 SSF51126, 2 hits
PROSITEiView protein in PROSITE
PS51484 G8, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCEMIP_MOUSE
AccessioniPrimary (citable) accession number: Q8BI06
Secondary accession number(s): E9QJS6
, Q6L9J4, Q9EPQ3, Q9EPQ4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 120 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
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