Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Due to a server migration, the UniProt 'ID mapping', 'Peptide search' and 'community bibliography submission' tools will not be available on the 19th April 2021 during the morning (EST).

UniProtKB - Q8BHY3 (ANO1_MOUSE)

Entry version 139 (07 Apr 2021)
Sequence version 2 (29 May 2007)
Previous versions | rss
Add a publicationFeedback
Protein

Anoctamin-1

Gene

Ano1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Calcium-activated chloride channel (CaCC) which plays an important role in transepithelial anion transport and smooth muscle contraction (PubMed:28561733, PubMed:29236691, PubMed:29236684). Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development.8 Publications

Miscellaneous

The term 'anoctamin' was coined because these channels are anion selective and are predicted to have eight (OCT) transmembrane segments. There is some dissatisfaction in the field with the Ano nomenclature because it is not certain that all the members of this family are anion channels or have the 8-transmembrane topology.Curated

Caution

Contains ten transmembrane regions, not eight as predicted.3 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

ATP and calmodulin are essential for its activation. Channel activity is inhibited by CFTR protein and by chloride inhibitors such as niflumic acid (NFA) and 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS) (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi651Calcium 11 Publication1
Metal bindingi654Calcium 21 Publication1
Metal bindingi702Calcium 21 Publication1
Metal bindingi705Calcium 11 Publication1
Metal bindingi734Calcium 11 Publication1
Metal bindingi738Calcium 21 Publication1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionChloride channel, Developmental protein, Ion channel
Biological processIon transport, Transport
LigandCalcium, Chloride, Metal-binding

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-MMU-2672351, Stimuli-sensing channels

Protein family/group databases

Transport Classification Database

More...TCDBi		1.A.17.1.25, the calcium-dependent chloride channel (ca-clc) family

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Anoctamin-1
Alternative name(s):
Transmembrane protein 16A
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:Ano1
Synonyms:Tmem16a
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMus musculus (Mouse)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri10090 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000589 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 7

Organism-specific databases

Mouse genome database (MGD) from Mouse Genome Informatics (MGI)

More...MGIi		MGI:2142149, Ano1

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the subcellular compartment where each non-membrane region of a membrane-spanning protein is found.<p><a href='/help/topo_dom' target='_top'>More...</a></p>Topological domaini1 – 333Cytoplasmic2 PublicationsAdd BLAST333
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei334 – 354Helical2 PublicationsAdd BLAST21
Topological domaini355 – 406Extracellular2 PublicationsAdd BLAST52
Transmembranei407 – 427Helical2 PublicationsAdd BLAST21
Topological domaini428 – 493Cytoplasmic2 PublicationsAdd BLAST66
Transmembranei494 – 514Helical2 PublicationsAdd BLAST21
Topological domaini515 – 542Extracellular2 PublicationsAdd BLAST28
Transmembranei543 – 563Helical2 PublicationsAdd BLAST21
Topological domaini564 – 581Cytoplasmic2 PublicationsAdd BLAST18
Transmembranei582 – 602Helical2 PublicationsAdd BLAST21
Topological domaini603 – 631Extracellular2 PublicationsAdd BLAST29
Transmembranei632 – 652Helical2 PublicationsAdd BLAST21
Topological domaini653 – 699Cytoplasmic2 PublicationsAdd BLAST47
Transmembranei700 – 720Helical2 PublicationsAdd BLAST21
Transmembranei721 – 741Helical2 PublicationsAdd BLAST21
Topological domaini742 – 758Cytoplasmic2 PublicationsAdd BLAST17
Transmembranei759 – 779Helical2 PublicationsAdd BLAST21
Topological domaini780 – 866Extracellular2 PublicationsAdd BLAST87
Transmembranei867 – 887Helical2 PublicationsAdd BLAST21
Topological domaini888 – 960Cytoplasmic2 PublicationsAdd BLAST73

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Mice display severe tracheomalacia with gaps in the tracheal cartilage rings along the entire length of the trachea. 90% of mutants die within the first nine days of postnatal life and no mutants survive longer than 30 days postpartum. Knockdown of Ano1 reduces calcium-activated chloride currents and saliva production.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi515R → A: Decreased permeability to chloride ions. 1 Publication1
Mutagenesisi535R → A: Decreased permeability to chloride ions. 1 Publication1
Mutagenesisi546N → D: Decreased threshold for activation by calcium. 1 Publication1
Mutagenesisi550I → A: Low constitutive channel activity. Decreased threshold for activation by calcium. 2 Publications1
Mutagenesisi588K → A or Q: Decreased permeability to chloride ions. 2 Publications1
Mutagenesisi591N → A: Increased permeability to chloride ions. 1 Publication1
Mutagenesisi593Y → D: Decreased threshold for activation by calcium. 1 Publication1
Mutagenesisi596I → A: Decreased threshold for activation by calcium. 1 Publication1
Mutagenesisi599V → A: Increased threshold for activation by calcium. Increased permeability to chloride ions. 1 Publication1
Mutagenesisi599V → K, L or R: Increased permeability to chloride ions. 1 Publication1
Mutagenesisi621R → E: Reduced anion permeability and increased cation permeability. 1 Publication1
Mutagenesisi625C → A: No effect of cysteine-modifying agent MTSET on ion permeability in contrast to wild-type where MTSET blocks current. 1 Publication1
Mutagenesisi630C → A: No effect of cysteine-modifying agent MTSET on ion permeability in contrast to wild-type where MTSET blocks current. 1 Publication1
Mutagenesisi635C → A: No effect of cysteine-modifying agent MTSET on ion permeability in contrast to wild-type where MTSET blocks current. 1 Publication1
Mutagenesisi639S → A: Decreased permeability to chloride ions. 1 Publication1
Mutagenesisi643L → A: Increased threshold for activation by calcium. 1 Publication1
Mutagenesisi644G → A: Decreased threshold for activation by calcium. 1 Publication1
Mutagenesisi644G → P: Low constitutive channel activity. Decreased threshold for activation by calcium. 1 Publication1
Mutagenesisi645K → A: Decreased permeability to chloride ions. 1 Publication1
Mutagenesisi645K → E: Reduced anion permeability and increased cation permeability. 1 Publication1
Mutagenesisi651N → A: Strongly increased threshold for activation by calcium. 1 Publication1
Mutagenesisi654E → A: Strongly increased threshold for activation by calcium. 1 Publication1
Mutagenesisi656G → A or P: No effect on threshold for activation by calcium. 1 Publication1
Mutagenesisi658P → A or G: Moderately increased threshold for activation by calcium. 1 Publication1
Mutagenesisi668K → E: Reduced anion permeability and increased cation permeability. 1 Publication1
Mutagenesisi709Q → A: Increased permeability to chloride ions. 1 Publication1
Mutagenesisi712F → A: Decreased threshold for activation by calcium. 1 Publication1
Mutagenesisi716F → A: Increased permeability to chloride ions. 1 Publication1
Mutagenesisi730N → A: Strongly increased threshold for activation by calcium. 1 Publication1
Mutagenesisi784D → A: Increased permeability to chloride ions. 1 Publication1
Mutagenesisi791Y → A: Increased permeability to chloride ions. 1 Publication1
Mutagenesisi807H → A: Increased permeability to chloride ions. 1 Publication1

Chemistry databases

ChEMBL database of bioactive drug-like small molecules

More...ChEMBLi		CHEMBL4105874

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002884361 – 960Anoctamin-1Add BLAST960

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei196PhosphoserineBy similarity1
<p>This subsection of the PTM / Processing":/help/ptm_processing_section section describes the positions of cysteine residues participating in disulfide bonds.<p><a href='/help/disulfid' target='_top'>More...</a></p>Disulfide bondi370 ↔ 395Combined sources1 Publication
Disulfide bondi379 ↔ 836Combined sources1 Publication
Disulfide bondi382 ↔ 386Combined sources1 Publication
Disulfide bondi625 ↔ 630Combined sources1 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM / Processing</a> section specifies the position and type of each covalently attached glycan group (mono-, di-, or polysaccharide).<p><a href='/help/carbohyd' target='_top'>More...</a></p>Glycosylationi806N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		Q8BHY3

PRoteomics IDEntifications database

More...PRIDEi		Q8BHY3

ProteomicsDB: a multi-organism proteome resource

More...ProteomicsDBi		296309 [Q8BHY3-1]
296310 [Q8BHY3-2]

PTM databases

GlyGen: Computational and Informatics Resources for Glycoscience

More...GlyGeni		Q8BHY3, 1 site

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		Q8BHY3

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		Q8BHY3

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Highly expressed in pulmonary bronchiole epithelial cells, pancreatic and submandibular gland acinar cells, kidney proximal tubule, all retinal cell layers, most sensory cells of dorsal root ganglia, Leydig cells and spermatocytes (at protein level). Expressed at high levels in the thyroid gland and gastrointestinal muscles.3 Publications

<p>This subsection of the 'Expression' section provides information on the expression of the gene product at various stages of a cell, tissue or organism development. By default, the information is derived from experiments at the mRNA level, unless specified 'at the protein level'.<p><a href='/help/developmental_stage' target='_top'>More...</a></p>Developmental stagei

In the developing respiratory system, expressed in epithelium of trachea and lung at 12.5 dpc and 14.5 dpc but not detected in distal epithelial tips. Expressed in the mesenchyme adjacent to the proximal conducting airway epithelium at 14.5 dpc but not at 16.5 dpc. Epithelial expression persists at 16.5 dpc. At 18.5 dpc, high levels detected only in epithelial cells of terminal sacules. In the developing gastrointestinal tract, expressed in the esophageal mesenchyme and epithelium of posterior stomach and intestine. In the developing skeleton, expressed in the perichondria of the neural arch of developing vertebrae at 14.5 dpc and 16.5 dpc. In developing skin, expression is restricted to basal layers of the epidermis at 16.5 dpc.1 Publication

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSMUSG00000031075, Expressed in parotid gland and 353 other tissues

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		Q8BHY3, MM

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homodimer (PubMed:28561733, PubMed:29236691, PubMed:29236684).

Interacts with CFTR (By similarity).

Interacts with TRPV4 (PubMed:24509911).

By similarity4 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Q8BHY3
With#Exp.IntAct
itself2EBI-11795280,EBI-11795280

GO - Molecular functioni

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

Protein interaction database and analysis system

More...IntActi		Q8BHY3, 1 interactor

STRING: functional protein association networks

More...STRINGi		10090.ENSMUSP00000112616

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		Q8BHY3, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		Q8BHY3

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The region spanning the fifth and sixth transmembrane domains probably forms the pore-forming region.

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the anoctamin family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG2514, Eukaryota

Ensembl GeneTree

More...GeneTreei		ENSGT00940000157182

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_006685_1_2_1

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		Q8BHY3

Database of Orthologous Groups

More...OrthoDBi		1263362at2759

Database for complete collections of gene phylogenies

More...PhylomeDBi		Q8BHY3

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR032394, Anoct_dimer
IPR007632, Anoctamin
IPR031287, Anoctamin-1

The PANTHER Classification System

More...PANTHERi		PTHR12308, PTHR12308, 1 hit
PTHR12308:SF13, PTHR12308:SF13, 1 hit

Pfam protein domain database

More...Pfami		View protein in Pfam
PF16178, Anoct_dimer, 1 hit
PF04547, Anoctamin, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 2 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform. This section is only present in reviewed entries, i.e. in UniProtKB/Swiss-Prot.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8BHY3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MRVPEKYSTL PAEDRSVHIV NICAIEDLGY LPSEGTLLNS LSVDPDAECK 
        60         70         80         90        100
YGLYFRDGKR KVDYILVYHH KRASGSRTLA RRGLQNDMVL GTRSVRQDQP 
       110        120        130        140        150
LPGKGSPVDA GSPEVPMDYH EDDKRFRREE YEGNLLEAGL ELENDEDTKI 
       160        170        180        190        200
HGVGFVKIHA PWHVLCREAE FLKLKMPTKK VYHISETRGL LKTINSVLQK 
       210        220        230        240        250
ITDPIQPKVA EHRPQTTKRL SYPFSREKQH LFDLTDRDSF FDSKTRSTIV 
       260        270        280        290        300
YEILKRTTCT KAKYSMGITS LLANGVYSAA YPLHDGDYEG DNVEFNDRKL 
       310        320        330        340        350
LYEEWASYGV FYKYQPIDLV RKYFGEKVGL YFAWLGAYTQ MLIPASIVGV 
       360        370        380        390        400
IVFLYGCATV DENIPSMEMC DQRYNITMCP LCDKTCSYWK MSSACATARA 
       410        420        430        440        450
SHLFDNPATV FFSVFMALWA ATFMEHWKRK QMRLNYRWDL TGFEEEEEAV 
       460        470        480        490        500
KDHPRAEYEA RVLEKSLRKE SRNKETDKVK LTWRDRFPAY FTNLVSIIFM 
       510        520        530        540        550
IAVTFAIVLG VIIYRISTAA ALAMNSSPSV RSNIRVTVTA TAVIINLVVI 
       560        570        580        590        600
ILLDEVYGCI ARWLTKIEVP KTEKSFEERL TFKAFLLKFV NSYTPIFYVA 
       610        620        630        640        650
FFKGRFVGRP GDYVYIFRSF RMEECAPGGC LMELCIQLSI IMLGKQLIQN 
       660        670        680        690        700
NLFEIGIPKM KKFIRYLKLR RQSPSDREEY VKRKQRYEVD FNLEPFAGLT 
       710        720        730        740        750
PEYMEMIIQF GFVTLFVASF PLAPLFALLN NIIEIRLDAK KFVTELRRPV 
       760        770        780        790        800
AIRAKDIGIW YNILRGVGKL AVIINAFVIS FTSDFIPRLV YLYMYSQNGT 
       810        820        830        840        850
MHGFVNHTLS SFNVSDFQNG TAPNDPLDLG YEVQICRYKD YREPPWSEHK 
       860        870        880        890        900
YDISKDFWAV LAARLAFVIV FQNLVMFMSD FVDWVIPDIP KDISQQIHKE 
       910        920        930        940        950
KVLMVELFMR EEQGKQQLLD TWMEKEKPRD VPCNNHSPTT HPEAGDGSPV 
       960
PSYEYHGDAL
Length:960
Mass (Da):110,916
Last modified:May 29, 2007 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iBFD0112FD310CE88
GO
Isoform 2 (identifier: Q8BHY3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     448-451: Missing.

Show »
Length:956
Mass (Da):110,489
Checksum:i150FACCDBDA4AF25
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A5F8MPM2A0A5F8MPM2_MOUSE
Anoctamin
Ano1
1,040Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
G8JL82G8JL82_MOUSE
Anoctamin
Ano1
1,014Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A0A0A0MQF2A0A0A0MQF2_MOUSE
Anoctamin
Ano1
1,017Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
F6R6E1F6R6E1_MOUSE
Anoctamin
Ano1
335Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
D3YYA4D3YYA4_MOUSE
Anoctamin-1
Ano1
230Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
D3Z035D3Z035_MOUSE
Anoctamin-1
Ano1
290Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAH06062 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence BAC26230 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence BAC26398 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence BAC35051 differs from that shown. Reason: Erroneous translation. Wrong choice of frame.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_025672448 – 451Missing in isoform 2. 1 Publication4

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
AK028991 mRNA Translation: BAC26230.1 Different initiation.
AK029329 mRNA Translation: BAC26398.1 Different initiation.
AK052589 mRNA Translation: BAC35051.1 Sequence problems.
BC006062 mRNA Translation: AAH06062.1 Different initiation.
BC062959 mRNA Translation: AAH62959.1

The Consensus CDS (CCDS) project

More...CCDSi		CCDS40202.2 [Q8BHY3-1]
CCDS57600.1 [Q8BHY3-2]

NCBI Reference Sequences

More...RefSeqi		NP_001229278.1, NM_001242349.1 [Q8BHY3-2]
NP_848757.4, NM_178642.5 [Q8BHY3-1]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...Ensembli		ENSMUST00000033393; ENSMUSP00000033393; ENSMUSG00000031075 [Q8BHY3-2]

Database of genes from NCBI RefSeq genomes

More...GeneIDi		101772

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		mmu:101772

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK028991 mRNA Translation: BAC26230.1 Different initiation.
AK029329 mRNA Translation: BAC26398.1 Different initiation.
AK052589 mRNA Translation: BAC35051.1 Sequence problems.
BC006062 mRNA Translation: AAH06062.1 Different initiation.
BC062959 mRNA Translation: AAH62959.1
CCDSiCCDS40202.2 [Q8BHY3-1]
CCDS57600.1 [Q8BHY3-2]
RefSeqiNP_001229278.1, NM_001242349.1 [Q8BHY3-2]
NP_848757.4, NM_178642.5 [Q8BHY3-1]

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5NL2electron microscopy6.60A/B1-960[»]
5OYBelectron microscopy3.75A/B1-960[»]
5OYGelectron microscopy4.06A/B1-960[»]
6BGIelectron microscopy3.80A/B1-907[»]
6BGJelectron microscopy3.80A/B1-907[»]
SMRiQ8BHY3
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

IntActiQ8BHY3, 1 interactor
STRINGi10090.ENSMUSP00000112616

Chemistry databases

ChEMBLiCHEMBL4105874

Protein family/group databases

TCDBi1.A.17.1.25, the calcium-dependent chloride channel (ca-clc) family

PTM databases

GlyGeniQ8BHY3, 1 site
iPTMnetiQ8BHY3
PhosphoSitePlusiQ8BHY3

Proteomic databases

PaxDbiQ8BHY3
PRIDEiQ8BHY3
ProteomicsDBi296309 [Q8BHY3-1]
296310 [Q8BHY3-2]

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...Antibodypediai		30667, 1332 antibodies

Genome annotation databases

EnsembliENSMUST00000033393; ENSMUSP00000033393; ENSMUSG00000031075 [Q8BHY3-2]
GeneIDi101772
KEGGimmu:101772

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		55107
MGIiMGI:2142149, Ano1

Phylogenomic databases

eggNOGiKOG2514, Eukaryota
GeneTreeiENSGT00940000157182
HOGENOMiCLU_006685_1_2_1
InParanoidiQ8BHY3
OrthoDBi1263362at2759
PhylomeDBiQ8BHY3

Enzyme and pathway databases

ReactomeiR-MMU-2672351, Stimuli-sensing channels

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...BioGRID-ORCSi		101772, 0 hits in 50 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...ChiTaRSi		Ano1, mouse

Protein Ontology

More...PROi		PR:Q8BHY3
RNActiQ8BHY3, protein

The Stanford Online Universal Resource for Clones and ESTs

More...SOURCEi		Search...

Gene expression databases

BgeeiENSMUSG00000031075, Expressed in parotid gland and 353 other tissues
GenevisibleiQ8BHY3, MM

Family and domain databases

InterProiView protein in InterPro
IPR032394, Anoct_dimer
IPR007632, Anoctamin
IPR031287, Anoctamin-1
PANTHERiPTHR12308, PTHR12308, 1 hit
PTHR12308:SF13, PTHR12308:SF13, 1 hit
PfamiView protein in Pfam
PF16178, Anoct_dimer, 1 hit
PF04547, Anoctamin, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiANO1_MOUSE
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q8BHY3
Secondary accession number(s): Q6P5C6, Q8BI26, Q99JK1
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: April 7, 2021
This is version 139 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again