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Protein

Anoctamin-1

Gene

Ano1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Calcium-activated chloride channel (CaCC) which plays an important role in transepithelial anion transport and smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development.5 Publications

Miscellaneous

The term 'anoctamin' was coined because these channels are anion selective and have eight (OCT) transmembrane segments. There is some dissatisfaction in the field with the Ano nomenclature because it is not certain that all the members of this family are anion channels or have the 8-transmembrane topology.

Activity regulationi

ATP and calmodulin are essential for its activation. Channel activity is inhibited by CFTR protein and by chloride inhibitors such as niflumic acid (NFA) and 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS) (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChloride channel, Developmental protein, Ion channel
Biological processIon transport, Transport
LigandCalcium, Chloride

Enzyme and pathway databases

ReactomeiR-MMU-2672351 Stimuli-sensing channels

Protein family/group databases

TCDBi1.A.17.1.25 the calcium-dependent chloride channel (ca-clc) family

Names & Taxonomyi

Protein namesi
Recommended name:
Anoctamin-1
Alternative name(s):
Transmembrane protein 16A
Gene namesi
Name:Ano1
Synonyms:Tmem16a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2142149 Ano1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 333CytoplasmicSequence analysisAdd BLAST333
Transmembranei334 – 354HelicalSequence analysisAdd BLAST21
Topological domaini355 – 402ExtracellularSequence analysisAdd BLAST48
Transmembranei403 – 423HelicalSequence analysisAdd BLAST21
Topological domaini424 – 493CytoplasmicSequence analysisAdd BLAST70
Transmembranei494 – 514HelicalSequence analysisAdd BLAST21
Topological domaini515 – 539ExtracellularSequence analysisAdd BLAST25
Transmembranei540 – 560HelicalSequence analysisAdd BLAST21
Topological domaini561 – 581CytoplasmicSequence analysisAdd BLAST21
Transmembranei582 – 602HelicalSequence analysisAdd BLAST21
Topological domaini603 – 705ExtracellularSequence analysisAdd BLAST103
Transmembranei706 – 726HelicalSequence analysisAdd BLAST21
Topological domaini727 – 765CytoplasmicSequence analysisAdd BLAST39
Transmembranei766 – 786HelicalSequence analysisAdd BLAST21
Topological domaini787 – 856ExtracellularSequence analysisAdd BLAST70
Transmembranei857 – 877HelicalSequence analysisAdd BLAST21
Topological domaini878 – 960CytoplasmicSequence analysisAdd BLAST83

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice display severe tracheomalacia with gaps in the tracheal cartilage rings along the entire length of the trachea. 90% of mutants die within the first nine days of postnatal life and no mutants survive longer than 30 days postpartum. Knockdown of Ano1 reduces calcium-activated chloride currents and saliva production.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi588K → Q: Decreased permeability to chloride ions. 1 Publication1
Mutagenesisi621R → E: Reduced anion permeability and increased cation permeability. 1 Publication1
Mutagenesisi625C → A: No effect of cysteine-modifying agent MTSET on ion permeability in contrast to wild-type where MTSET blocks current. 1 Publication1
Mutagenesisi630C → A: No effect of cysteine-modifying agent MTSET on ion permeability in contrast to wild-type where MTSET blocks current. 1 Publication1
Mutagenesisi635C → A: No effect of cysteine-modifying agent MTSET on ion permeability in contrast to wild-type where MTSET blocks current. 1 Publication1
Mutagenesisi645K → E: Reduced anion permeability and increased cation permeability. 1 Publication1
Mutagenesisi668K → E: Reduced anion permeability and increased cation permeability. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002884361 – 960Anoctamin-1Add BLAST960

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei196PhosphoserineBy similarity1
Glycosylationi806N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ8BHY3
PRIDEiQ8BHY3

PTM databases

iPTMnetiQ8BHY3
PhosphoSitePlusiQ8BHY3

Expressioni

Tissue specificityi

Highly expressed in pulmonary bronchiole epithelial cells, pancreatic and submandibular gland acinar cells, kidney proximal tubule, all retinal cell layers, most sensory cells of dorsal root ganglia, Leydig cells and spermatocytes (at protein level). Expressed at high levels in the thyroid gland and gastrointestinal muscles.3 Publications

Developmental stagei

In the developing respiratory system, expressed in epithelium of trachea and lung at E12.5 and E14.5 but not detected in distal epithelial tips. Expressed in the mesenchyme adjacent to the proximal conducting airway epithelium at E14.5 but not at E16.5. Epithelial expression persists at E16.5. At E18.5, high levels detected only in epithelial cells of terminal sacules. In the developing gastrointestinal tract, expressed in the esophageal mesenchyme and epithelium of posterior stomach and intestine. In the developing skeleton, expressed in the perichondria of the neural arch of developing vertebrae at E14.5 and E16.5. In developing skin, expression is restricted to basal layers of the epidermis at E16.5.1 Publication

Gene expression databases

BgeeiENSMUSG00000031075 Expressed in 336 organ(s), highest expression level in parotid gland
ExpressionAtlasiQ8BHY3 baseline and differential
GenevisibleiQ8BHY3 MM

Interactioni

Subunit structurei

Homodimer. Interacts with CFTR (By similarity). Interacts with TRPV4 (PubMed:24509911).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ8BHY3, 1 interactor
STRINGi10090.ENSMUSP00000113899

Structurei

3D structure databases

ProteinModelPortaliQ8BHY3
SMRiQ8BHY3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The region spanning the fifth and sixth transmembrane domains probably forms the pore-forming region.

Sequence similaritiesi

Belongs to the anoctamin family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2514 Eukaryota
ENOG410XS4S LUCA
GeneTreeiENSGT00760000119015
HOGENOMiHOG000006509
HOVERGENiHBG069519
InParanoidiQ8BHY3
KOiK19496
PhylomeDBiQ8BHY3

Family and domain databases

InterProiView protein in InterPro
IPR032394 Anoct_dimer
IPR007632 Anoctamin
IPR031287 Anoctamin-1
PANTHERiPTHR12308 PTHR12308, 1 hit
PTHR12308:SF13 PTHR12308:SF13, 1 hit
PfamiView protein in Pfam
PF16178 Anoct_dimer, 1 hit
PF04547 Anoctamin, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8BHY3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MRVPEKYSTL PAEDRSVHIV NICAIEDLGY LPSEGTLLNS LSVDPDAECK
60 70 80 90 100
YGLYFRDGKR KVDYILVYHH KRASGSRTLA RRGLQNDMVL GTRSVRQDQP
110 120 130 140 150
LPGKGSPVDA GSPEVPMDYH EDDKRFRREE YEGNLLEAGL ELENDEDTKI
160 170 180 190 200
HGVGFVKIHA PWHVLCREAE FLKLKMPTKK VYHISETRGL LKTINSVLQK
210 220 230 240 250
ITDPIQPKVA EHRPQTTKRL SYPFSREKQH LFDLTDRDSF FDSKTRSTIV
260 270 280 290 300
YEILKRTTCT KAKYSMGITS LLANGVYSAA YPLHDGDYEG DNVEFNDRKL
310 320 330 340 350
LYEEWASYGV FYKYQPIDLV RKYFGEKVGL YFAWLGAYTQ MLIPASIVGV
360 370 380 390 400
IVFLYGCATV DENIPSMEMC DQRYNITMCP LCDKTCSYWK MSSACATARA
410 420 430 440 450
SHLFDNPATV FFSVFMALWA ATFMEHWKRK QMRLNYRWDL TGFEEEEEAV
460 470 480 490 500
KDHPRAEYEA RVLEKSLRKE SRNKETDKVK LTWRDRFPAY FTNLVSIIFM
510 520 530 540 550
IAVTFAIVLG VIIYRISTAA ALAMNSSPSV RSNIRVTVTA TAVIINLVVI
560 570 580 590 600
ILLDEVYGCI ARWLTKIEVP KTEKSFEERL TFKAFLLKFV NSYTPIFYVA
610 620 630 640 650
FFKGRFVGRP GDYVYIFRSF RMEECAPGGC LMELCIQLSI IMLGKQLIQN
660 670 680 690 700
NLFEIGIPKM KKFIRYLKLR RQSPSDREEY VKRKQRYEVD FNLEPFAGLT
710 720 730 740 750
PEYMEMIIQF GFVTLFVASF PLAPLFALLN NIIEIRLDAK KFVTELRRPV
760 770 780 790 800
AIRAKDIGIW YNILRGVGKL AVIINAFVIS FTSDFIPRLV YLYMYSQNGT
810 820 830 840 850
MHGFVNHTLS SFNVSDFQNG TAPNDPLDLG YEVQICRYKD YREPPWSEHK
860 870 880 890 900
YDISKDFWAV LAARLAFVIV FQNLVMFMSD FVDWVIPDIP KDISQQIHKE
910 920 930 940 950
KVLMVELFMR EEQGKQQLLD TWMEKEKPRD VPCNNHSPTT HPEAGDGSPV
960
PSYEYHGDAL
Length:960
Mass (Da):110,916
Last modified:May 29, 2007 - v2
Checksum:iBFD0112FD310CE88
GO
Isoform 2 (identifier: Q8BHY3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     448-451: Missing.

Show »
Length:956
Mass (Da):110,489
Checksum:i150FACCDBDA4AF25
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0A0MQF2A0A0A0MQF2_MOUSE
Anoctamin
Ano1
1,017Annotation score:
G8JL82G8JL82_MOUSE
Anoctamin
Ano1
1,014Annotation score:
F6R6E1F6R6E1_MOUSE
Anoctamin
Ano1
335Annotation score:
D3YYA4D3YYA4_MOUSE
Anoctamin-1
Ano1
230Annotation score:
D3Z035D3Z035_MOUSE
Anoctamin-1
Ano1
290Annotation score:

Sequence cautioni

The sequence AAH06062 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAC26230 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAC26398 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAC35051 differs from that shown. Reason: Erroneous translation. Wrong choice of frame.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_025672448 – 451Missing in isoform 2. 1 Publication4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK028991 mRNA Translation: BAC26230.1 Different initiation.
AK029329 mRNA Translation: BAC26398.1 Different initiation.
AK052589 mRNA Translation: BAC35051.1 Sequence problems.
BC006062 mRNA Translation: AAH06062.1 Different initiation.
BC062959 mRNA Translation: AAH62959.1
RefSeqiNP_001229278.1, NM_001242349.1
NP_848757.4, NM_178642.5
UniGeneiMm.26700

Genome annotation databases

EnsembliENSMUST00000033393; ENSMUSP00000033393; ENSMUSG00000031075 [Q8BHY3-2]
GeneIDi101772
KEGGimmu:101772

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK028991 mRNA Translation: BAC26230.1 Different initiation.
AK029329 mRNA Translation: BAC26398.1 Different initiation.
AK052589 mRNA Translation: BAC35051.1 Sequence problems.
BC006062 mRNA Translation: AAH06062.1 Different initiation.
BC062959 mRNA Translation: AAH62959.1
RefSeqiNP_001229278.1, NM_001242349.1
NP_848757.4, NM_178642.5
UniGeneiMm.26700

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5NL2electron microscopy6.60A/B1-960[»]
5OYBelectron microscopy3.75A/B1-960[»]
5OYGelectron microscopy4.06A/B1-960[»]
6BGIelectron microscopy3.80A/B1-907[»]
6BGJelectron microscopy3.80A/B1-907[»]
ProteinModelPortaliQ8BHY3
SMRiQ8BHY3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BHY3, 1 interactor
STRINGi10090.ENSMUSP00000113899

Protein family/group databases

TCDBi1.A.17.1.25 the calcium-dependent chloride channel (ca-clc) family

PTM databases

iPTMnetiQ8BHY3
PhosphoSitePlusiQ8BHY3

Proteomic databases

PaxDbiQ8BHY3
PRIDEiQ8BHY3

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033393; ENSMUSP00000033393; ENSMUSG00000031075 [Q8BHY3-2]
GeneIDi101772
KEGGimmu:101772

Organism-specific databases

CTDi55107
MGIiMGI:2142149 Ano1

Phylogenomic databases

eggNOGiKOG2514 Eukaryota
ENOG410XS4S LUCA
GeneTreeiENSGT00760000119015
HOGENOMiHOG000006509
HOVERGENiHBG069519
InParanoidiQ8BHY3
KOiK19496
PhylomeDBiQ8BHY3

Enzyme and pathway databases

ReactomeiR-MMU-2672351 Stimuli-sensing channels

Miscellaneous databases

PROiPR:Q8BHY3
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031075 Expressed in 336 organ(s), highest expression level in parotid gland
ExpressionAtlasiQ8BHY3 baseline and differential
GenevisibleiQ8BHY3 MM

Family and domain databases

InterProiView protein in InterPro
IPR032394 Anoct_dimer
IPR007632 Anoctamin
IPR031287 Anoctamin-1
PANTHERiPTHR12308 PTHR12308, 1 hit
PTHR12308:SF13 PTHR12308:SF13, 1 hit
PfamiView protein in Pfam
PF16178 Anoct_dimer, 1 hit
PF04547 Anoctamin, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiANO1_MOUSE
AccessioniPrimary (citable) accession number: Q8BHY3
Secondary accession number(s): Q6P5C6, Q8BI26, Q99JK1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: September 12, 2018
This is version 120 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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