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Protein

Pre-mRNA-splicing factor RBM22

Gene

Rbm22

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the first step of pre-mRNA splicing. Binds directly to the internal stem-loop (ISL) domain of the U6 snRNA and to the pre-mRNA intron near the 5' splice site during the activation and catalytic phases of the spliceosome cycle. Involved in both translocations of the nuclear SLU7 to the cytoplasm and the cytosolic calcium-binding protein PDCD6 to the nucleus upon cellular stress responses (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri159 – 186C3H1-typePROSITE-ProRule annotationAdd BLAST28

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processmRNA processing, mRNA splicing, Transport
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-72163 mRNA Splicing - Major Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor RBM22
Alternative name(s):
RNA-binding motif protein 22
Gene namesi
Name:Rbm22
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1914060 Rbm22

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002505482 – 420Pre-mRNA-splicing factor RBM22Add BLAST419

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei4PhosphoserineBy similarity1
Modified residuei102PhosphoserineBy similarity1
Cross-linki139Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki149Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei212N6-acetyllysineCombined sources1
Cross-linki290Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8BHS3
MaxQBiQ8BHS3
PaxDbiQ8BHS3
PeptideAtlasiQ8BHS3
PRIDEiQ8BHS3

PTM databases

iPTMnetiQ8BHS3
PhosphoSitePlusiQ8BHS3

Expressioni

Gene expression databases

BgeeiENSMUSG00000024604 Expressed in 281 organ(s), highest expression level in neocortex
CleanExiMM_RBM22
GenevisibleiQ8BHS3 MM

Interactioni

Subunit structurei

Identified in the spliceosome C complex. Interacts with PDCD6; the interaction induces translocation of PDCD6 in the cytoplasm (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ8BHS3, 1 interactor
MINTiQ8BHS3
STRINGi10090.ENSMUSP00000025506

Structurei

3D structure databases

ProteinModelPortaliQ8BHS3
SMRiQ8BHS3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini232 – 305RRMPROSITE-ProRule annotationAdd BLAST74

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi327 – 407Pro-richAdd BLAST81

Domaini

The C-terminus RRM domain and the zinc finger motif are necessary for RNA-binding.By similarity

Sequence similaritiesi

Belongs to the SLT11 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri159 – 186C3H1-typePROSITE-ProRule annotationAdd BLAST28

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0153 Eukaryota
ENOG410XSFK LUCA
GeneTreeiENSGT00390000002792
HOGENOMiHOG000171086
HOVERGENiHBG083475
InParanoidiQ8BHS3
KOiK12872
OMAiVCSHMSH
OrthoDBiEOG091G0B2N
TreeFamiTF314284

Family and domain databases

Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR039171 Cwc2/Slt11
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
IPR000571 Znf_CCCH
IPR036855 Znf_CCCH_sf
PANTHERiPTHR14089 PTHR14089, 1 hit
PfamiView protein in Pfam
PF00076 RRM_1, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SM00356 ZnF_C3H1, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
SSF90229 SSF90229, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit
PS50103 ZF_C3H1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8BHS3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MATSLGSNTY NRQNWEDADF PILCQTCLGE NPYIRMTKEK YGKECKICAR
60 70 80 90 100
PFTVFRWCPG VRMRFKKTEV CQTCSKLKNV CQTCLLDLEY GLPIQVRDAG
110 120 130 140 150
LSFKDDMPKS DVNKEYYTQN MEREISNSDG TRPVGMLGKA TSTSDMLLKL
160 170 180 190 200
ARTTPYYKRN RPHICSFWVK GECKRGEECP YRHEKPTDPD DPLADQNIKD
210 220 230 240 250
RYYGINDPVA DKLLKRASTM PRLDPPEDKT ITTLYVGGLG DTITETDLRN
260 270 280 290 300
HFYQFGEIRT ITVVQRQQCA FIQFATRQAA EVAAEKSFNK LIVNGRRLNV
310 320 330 340 350
KWGRSQAARG KEKEKDGTTD SGIKLEPVPG LPGALPPPPA AEEEASANYF
360 370 380 390 400
NLPPSGPPAV VNIALPPPPG IAPPPPPGFG PHMFHPMGPP PPFMRAPGPI
410 420
HYPSQDPQRM GAHAGKHSSP
Length:420
Mass (Da):46,896
Last modified:March 1, 2003 - v1
Checksum:iA82549D8CC88C7D0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti171G → R in BAE39585 (PubMed:16141072).Curated1
Sequence conflicti339P → L in BAB31220 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK018452 mRNA Translation: BAB31220.1
AK045116 mRNA Translation: BAC32229.1
AK150271 mRNA Translation: BAE29427.1
AK151758 mRNA Translation: BAE30665.1
AK152252 mRNA Translation: BAE31073.1
AK166600 mRNA Translation: BAE38885.1
AK167509 mRNA Translation: BAE39585.1
AK168919 mRNA Translation: BAE40732.1
BC080205 mRNA Translation: AAH80205.1
CCDSiCCDS29272.1
RefSeqiNP_080052.1, NM_025776.2
UniGeneiMm.275106

Genome annotation databases

EnsembliENSMUST00000025506; ENSMUSP00000025506; ENSMUSG00000024604
GeneIDi66810
KEGGimmu:66810
UCSCiuc008fal.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK018452 mRNA Translation: BAB31220.1
AK045116 mRNA Translation: BAC32229.1
AK150271 mRNA Translation: BAE29427.1
AK151758 mRNA Translation: BAE30665.1
AK152252 mRNA Translation: BAE31073.1
AK166600 mRNA Translation: BAE38885.1
AK167509 mRNA Translation: BAE39585.1
AK168919 mRNA Translation: BAE40732.1
BC080205 mRNA Translation: AAH80205.1
CCDSiCCDS29272.1
RefSeqiNP_080052.1, NM_025776.2
UniGeneiMm.275106

3D structure databases

ProteinModelPortaliQ8BHS3
SMRiQ8BHS3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BHS3, 1 interactor
MINTiQ8BHS3
STRINGi10090.ENSMUSP00000025506

PTM databases

iPTMnetiQ8BHS3
PhosphoSitePlusiQ8BHS3

Proteomic databases

EPDiQ8BHS3
MaxQBiQ8BHS3
PaxDbiQ8BHS3
PeptideAtlasiQ8BHS3
PRIDEiQ8BHS3

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025506; ENSMUSP00000025506; ENSMUSG00000024604
GeneIDi66810
KEGGimmu:66810
UCSCiuc008fal.1 mouse

Organism-specific databases

CTDi55696
MGIiMGI:1914060 Rbm22

Phylogenomic databases

eggNOGiKOG0153 Eukaryota
ENOG410XSFK LUCA
GeneTreeiENSGT00390000002792
HOGENOMiHOG000171086
HOVERGENiHBG083475
InParanoidiQ8BHS3
KOiK12872
OMAiVCSHMSH
OrthoDBiEOG091G0B2N
TreeFamiTF314284

Enzyme and pathway databases

ReactomeiR-MMU-72163 mRNA Splicing - Major Pathway

Miscellaneous databases

ChiTaRSiRbm22 mouse
PROiPR:Q8BHS3
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024604 Expressed in 281 organ(s), highest expression level in neocortex
CleanExiMM_RBM22
GenevisibleiQ8BHS3 MM

Family and domain databases

Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR039171 Cwc2/Slt11
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
IPR000571 Znf_CCCH
IPR036855 Znf_CCCH_sf
PANTHERiPTHR14089 PTHR14089, 1 hit
PfamiView protein in Pfam
PF00076 RRM_1, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SM00356 ZnF_C3H1, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
SSF90229 SSF90229, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit
PS50103 ZF_C3H1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRBM22_MOUSE
AccessioniPrimary (citable) accession number: Q8BHS3
Secondary accession number(s): Q3TJB0, Q9CXA0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: March 1, 2003
Last modified: November 7, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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