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Protein

Neutral alpha-glucosidase AB

Gene

Ganab

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic subunit of glucosidase II that cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc2Man9GlcNAc2 oligosaccharide precursor of immature glycoproteins (PubMed:27462106). Required for PKD1/Polycystin-1 and PKD2/Polycystin-2 maturation and localization to the cell surface and cilia.By similarity1 Publication

Catalytic activityi

Hydrolysis of terminal (1->3)-alpha-D-glucosidic links in (1->3)-alpha-D-glucans.1 Publication

pH dependencei

Optimum pH is 6.5 - 7.

Pathwayi: N-glycan metabolism

This protein is involved in the pathway N-glycan metabolism, which is part of Glycan metabolism.1 Publication
View all proteins of this organism that are known to be involved in the pathway N-glycan metabolism and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei283SubstrateCombined sources1 Publication1
Binding sitei429SubstrateCombined sources1 Publication1
Active sitei542NucleophilePROSITE-ProRule annotationCombined sources1 Publication1
Binding sitei602SubstrateCombined sources1 Publication1
Active sitei618Proton donor1 Publication1
Binding sitei676SubstrateCombined sources1 Publication1

GO - Molecular functioni

  • carbohydrate binding Source: InterPro
  • glucan 1,3-alpha-glucosidase activity Source: UniProtKB
  • glucosidase activity Source: MGI

GO - Biological processi

  • carbohydrate metabolic process Source: InterPro
  • N-glycan processing Source: UniProtKB

Keywordsi

Molecular functionGlycosidase, Hydrolase

Enzyme and pathway databases

BRENDAi3.2.1.84 3474
ReactomeiR-MMU-532668 N-glycan trimming in the ER and Calnexin/Calreticulin cycle
R-MMU-901042 Calnexin/calreticulin cycle
UniPathwayiUPA00957

Protein family/group databases

CAZyiGH31 Glycoside Hydrolase Family 31

Names & Taxonomyi

Protein namesi
Recommended name:
Neutral alpha-glucosidase AB (EC:3.2.1.841 Publication)
Alternative name(s):
Alpha-glucosidase 2
Glucosidase II subunit alpha1 Publication
Gene namesi
Name:Ganab
Synonyms:G2an, Kiaa0088
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1097667 Ganab

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi542D → E or N: Loss of enzyme activity. 1 Publication1
Mutagenesisi618D → N: Loss of enzyme activity. 1 Publication1
Mutagenesisi818R → E: Disrupts interaction with PRKCSH. Nearly abolishes enzyme activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 321 PublicationAdd BLAST32
ChainiPRO_000001857233 – 944Neutral alpha-glucosidase ABAdd BLAST912

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi41 ↔ 47Combined sources1 Publication
Modified residuei52PhosphoserineBy similarity1
Glycosylationi97N-linked (GlcNAc...) asparagineCombined sources2 Publications1
Disulfide bondi633 ↔ 644Combined sources1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ8BHN3
PaxDbiQ8BHN3
PeptideAtlasiQ8BHN3
PRIDEiQ8BHN3

PTM databases

CarbonylDBiQ8BHN3
iPTMnetiQ8BHN3
PhosphoSitePlusiQ8BHN3
SwissPalmiQ8BHN3

Expressioni

Gene expression databases

BgeeiENSMUSG00000071650
CleanExiMM_GANAB
ExpressionAtlasiQ8BHN3 baseline and differential
GenevisibleiQ8BHN3 MM

Interactioni

Subunit structurei

Heterodimer of a catalytic alpha subunit (GANAB) and a beta subunit (PRKCSH) (PubMed:9148925, PubMed:10921916, PubMed:27462106). Binds glycosylated PTPRC (PubMed:9148925, PubMed:10921916).3 Publications

Protein-protein interaction databases

BioGridi199786, 1 interactor
IntActiQ8BHN3, 6 interactors
MINTiQ8BHN3
STRINGi10090.ENSMUSP00000093965

Structurei

Secondary structure

1944
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi35 – 37Combined sources3
Helixi41 – 43Combined sources3
Helixi45 – 51Combined sources7
Beta strandi59 – 69Combined sources11
Beta strandi74 – 80Combined sources7
Turni81 – 83Combined sources3
Beta strandi86 – 94Combined sources9
Turni95 – 97Combined sources3
Beta strandi98 – 107Combined sources10
Beta strandi117 – 121Combined sources5
Beta strandi128 – 133Combined sources6
Beta strandi135 – 142Combined sources8
Beta strandi145 – 152Combined sources8
Turni153 – 156Combined sources4
Beta strandi157 – 162Combined sources6
Beta strandi165 – 171Combined sources7
Beta strandi225 – 230Combined sources6
Beta strandi233 – 235Combined sources3
Beta strandi244 – 251Combined sources8
Beta strandi255 – 258Combined sources4
Beta strandi277 – 279Combined sources3
Beta strandi289 – 291Combined sources3
Beta strandi301 – 305Combined sources5
Beta strandi310 – 315Combined sources6
Beta strandi321 – 328Combined sources8
Beta strandi350 – 360Combined sources11
Beta strandi362 – 367Combined sources6
Helixi372 – 383Combined sources12
Helixi391 – 394Combined sources4
Beta strandi395 – 398Combined sources4
Helixi406 – 418Combined sources13
Beta strandi424 – 428Combined sources5
Helixi430 – 432Combined sources3
Turni443 – 445Combined sources3
Helixi449 – 458Combined sources10
Beta strandi462 – 466Combined sources5
Helixi478 – 485Combined sources8
Beta strandi495 – 497Combined sources3
Beta strandi500 – 502Combined sources3
Beta strandi505 – 508Combined sources4
Helixi515 – 524Combined sources10
Turni527 – 529Combined sources3
Beta strandi537 – 541Combined sources5
Turni544 – 546Combined sources3
Helixi552 – 554Combined sources3
Helixi563 – 565Combined sources3
Helixi568 – 571Combined sources4
Helixi572 – 574Combined sources3
Helixi575 – 589Combined sources15
Turni590 – 592Combined sources3
Beta strandi599 – 603Combined sources5
Helixi608 – 611Combined sources4
Beta strandi613 – 615Combined sources3
Beta strandi620 – 622Combined sources3
Helixi623 – 638Combined sources16
Beta strandi643 – 645Combined sources3
Beta strandi651 – 653Combined sources3
Helixi657 – 667Combined sources11
Beta strandi670 – 675Combined sources6
Helixi686 – 688Combined sources3
Helixi691 – 706Combined sources16
Helixi708 – 721Combined sources14
Beta strandi725 – 727Combined sources3
Helixi729 – 732Combined sources4
Helixi737 – 739Combined sources3
Beta strandi746 – 748Combined sources3
Turni749 – 751Combined sources3
Beta strandi752 – 754Combined sources3
Beta strandi764 – 769Combined sources6
Beta strandi775 – 778Combined sources4
Turni779 – 781Combined sources3
Beta strandi784 – 794Combined sources11
Beta strandi802 – 805Combined sources4
Beta strandi808 – 812Combined sources5
Helixi820 – 823Combined sources4
Beta strandi828 – 833Combined sources6
Beta strandi840 – 846Combined sources7
Beta strandi849 – 851Combined sources3
Helixi853 – 856Combined sources4
Beta strandi861 – 868Combined sources8
Beta strandi871 – 877Combined sources7
Beta strandi888 – 895Combined sources8
Beta strandi901 – 907Combined sources7
Beta strandi917 – 920Combined sources4
Turni921 – 924Combined sources4
Beta strandi925 – 929Combined sources5
Beta strandi939 – 944Combined sources6

3D structure databases

ProteinModelPortaliQ8BHN3
SMRiQ8BHN3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 31 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1066 Eukaryota
COG1501 LUCA
GeneTreeiENSGT00760000119229
HOGENOMiHOG000115864
HOVERGENiHBG051683
InParanoidiQ8BHN3
KOiK05546
OMAiTVHQPLW
PhylomeDBiQ8BHN3
TreeFamiTF300337

Family and domain databases

Gene3Di2.60.40.1180, 2 hits
InterProiView protein in InterPro
IPR011013 Gal_mutarotase_sf_dom
IPR000322 Glyco_hydro_31
IPR030458 Glyco_hydro_31_AS
IPR030459 Glyco_hydro_31_CS
IPR025887 Glyco_hydro_31_N_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF13802 Gal_mutarotas_2, 1 hit
PF01055 Glyco_hydro_31, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
SSF74650 SSF74650, 2 hits
PROSITEiView protein in PROSITE
PS00129 GLYCOSYL_HYDROL_F31_1, 1 hit
PS00707 GLYCOSYL_HYDROL_F31_2, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BHN3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAIAAVAAR RRRSWLSLVL AYLGVCLGIT LAVDRSNFKT CDESSFCKRQ
60 70 80 90 100
RSIRPGLSPY RALLDTLQLG PDALTVHLIH EVTKVLLVLE LQGLQKNMTR
110 120 130 140 150
IRIDELEPRR PRYRVPDVLV ADPPTARLSV SGRDDNSVEL TVAEGPYKII
160 170 180 190 200
LTAQPFRLDL LEDRSLLLSV NARGLMAFEH QRAPRVPQES KDPAEGNGAQ
210 220 230 240 250
PEATPGDGDK PEETQEKAEK DEPGAWEETF KTHSDSKPYG PTSVGLDFSL
260 270 280 290 300
PGMEHVYGIP EHADSLRLKV TEGGEPYRLY NLDVFQYELN NPMALYGSVP
310 320 330 340 350
VLLAHSFHRD LGIFWLNAAE TWVDISSNTA GKTLFGKMLD YLQGSGETPQ
360 370 380 390 400
TDIRWMSESG IIDVFLMLGP SVFDVFRQYA SLTGTQALPP LFSLGYHQSR
410 420 430 440 450
WNYRDEADVL EVDQGFDDHN MPCDVIWLDI EHADGKRYFT WDPTRFPQPL
460 470 480 490 500
NMLEHLASKR RKLVAIVDPH IKVDSGYRVH EELRNHGLYV KTRDGSDYEG
510 520 530 540 550
WCWPGSASYP DFTNPRMRAW WSNMFSFDNY EGSAPNLYVW NDMNEPSVFN
560 570 580 590 600
GPEVTMLKDA VHYGGWEHRD IHNIYGLYVH MATADGLIQR SGGIERPFVL
610 620 630 640 650
SRAFFSGSQR FGAVWTGDNT AEWDHLKISI PMCLSLALVG LSFCGADVGG
660 670 680 690 700
FFKNPEPELL VRWYQMGAYQ PFFRAHAHLD TGRREPWLLA SQYQDAIRDA
710 720 730 740 750
LFQRYSLLPF WYTLFYQAHK EGFPVMRPLW VQYPEDMSTF SIEDQFMLGD
760 770 780 790 800
ALLIHPVSDA GAHGVQVYLP GQEEVWYDIQ SYQKHHGPQT LYLPVTLSSI
810 820 830 840 850
PVFQRGGTIV PRWMRVRRSS DCMKDDPITL FVALSPQGTA QGELFLDDGH
860 870 880 890 900
TFNYQTRHEF LLRRFSFSGS TLVSSSADPK GHLETPIWIE RVVIMGAGKP
910 920 930 940
AAVVLQTKGS PESRLSFQHD PETSVLILRK PGVSVASDWS IHLR
Length:944
Mass (Da):106,911
Last modified:March 1, 2003 - v1
Checksum:i01489861A537416E
GO
Isoform 2 (identifier: Q8BHN3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     187-187: P → PFSDKVSLALGSVWDKIKNLFSR

Show »
Length:966
Mass (Da):109,404
Checksum:iE3E507E2E2E68C75
GO
Isoform 3 (identifier: Q8BHN3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-252: Missing.

Note: May be due to an intron retention.
Show »
Length:692
Mass (Da):79,082
Checksum:iE4877A7B38966E3C
GO

Sequence cautioni

The sequence BAC65483 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti647D → N in BAB30982 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0106751 – 252Missing in isoform 3. 1 PublicationAdd BLAST252
Alternative sequenceiVSP_010676187P → PFSDKVSLALGSVWDKIKNL FSR in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U92793 mRNA Translation: AAC53182.1
AK017873 mRNA Translation: BAB30982.1
AK030722 mRNA Translation: BAC27099.1
AK081915 mRNA Translation: BAC38370.1
AK122201 mRNA Translation: BAC65483.1 Different initiation.
BC094437 mRNA Translation: AAH94437.1
BC117888 mRNA Translation: AAI17889.1
BC117889 mRNA Translation: AAI17890.1
CCDSiCCDS29557.1 [Q8BHN3-2]
RefSeqiNP_001280550.1, NM_001293621.1 [Q8BHN3-1]
NP_032086.1, NM_008060.2 [Q8BHN3-2]
UniGeneiMm.3196

Genome annotation databases

EnsembliENSMUST00000096246; ENSMUSP00000093965; ENSMUSG00000071650 [Q8BHN3-2]
GeneIDi14376
KEGGimmu:14376
UCSCiuc008gnx.2 mouse [Q8BHN3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGANAB_MOUSE
AccessioniPrimary (citable) accession number: Q8BHN3
Secondary accession number(s): O08794
, Q149A6, Q80U81, Q9CS53
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: March 1, 2003
Last modified: July 18, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

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