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Protein

SLAM family member 7

Gene

Slamf7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2 (PubMed:19648922). Mediates natural killer (NK) cell activation through a SH2D1A-independent extracellular signal-regulated ERK-mediated pathway (By similarity). Positively regulates NK cell functions by a mechanism dependent on the adapter SH2D1B. In addition to heterotypic NK cells-target cells interactions also homotypic interactions between NK cells may contribute to activation. However, in the absence of SH2D1B, inhibits NK cell function. Acts also inhibitory in T-cells (PubMed:19151721). May play a role in lymphocyte adhesion (By similarity). In LPS-activated monocytes negatively regulates production of proinflammatory cytokines (By similarity).By similarity2 Publications

Miscellaneous

CRACC:CRACC interaction between NK cells and resident Kupffer cells contribute to Poly I:C/D-GalN-induced fulminant hepatitis.1 Publication

GO - Molecular functioni

GO - Biological processi

  • adaptive immune response Source: UniProtKB-KW
  • innate immune response Source: UniProtKB-KW
  • regulation of natural killer cell activation Source: MGI

Keywordsi

Molecular functionReceptor
Biological processAdaptive immunity, Immunity, Innate immunity

Names & Taxonomyi

Protein namesi
Recommended name:
SLAM family member 7
Alternative name(s):
Leukocyte cell-surface antigen
Novel Ly9
CD_antigen: CD319
Gene namesi
Name:Slamf7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1922595 Slamf7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 224ExtracellularSequence analysisAdd BLAST202
Transmembranei225 – 245HelicalSequence analysisAdd BLAST21
Topological domaini246 – 333CytoplasmicSequence analysisAdd BLAST88

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi282Y → F: No effect on interaction with SH2D1B, increases NK cell activation in presence of SH2D1B, abolishes NK cell inhibition in absence of SH2D1B. 1 Publication1
Mutagenesisi287Y → F: No effect on interaction with SH2D1, no effect on NK cell activation in presence of SH2D1B, no effect on NK cell inhibition in absence of SH2D1B. 1 Publication1
Mutagenesisi302Y → F: Disrupts interaction with SH2D1B, abolishes NK cell activation in presence of SH2D1B, no effect on NK cell inhibition in absence of SH2D1B. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22By similarityAdd BLAST22
ChainiPRO_000001496423 – 333SLAM family member 7Add BLAST311

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi42N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi95N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi139N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi142 ↔ 212PROSITE-ProRule annotation
Glycosylationi145N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi148 ↔ 192PROSITE-ProRule annotation
Glycosylationi156N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei282Phosphotyrosine1 Publication1
Modified residuei302Phosphotyrosine1 Publication1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ8BHK6
PaxDbiQ8BHK6
PRIDEiQ8BHK6

PTM databases

iPTMnetiQ8BHK6
PhosphoSitePlusiQ8BHK6

Expressioni

Tissue specificityi

Expressed in spleen, lymph node, bone marrow and testis. Lower levels detected in thymus. Expressed in NK cells, B-cells, natural killer cells and activated T-cells.2 Publications

Gene expression databases

CleanExiMM_SLAMF7

Interactioni

Subunit structurei

Interacts (via ITSM phosphorylated on Tyr-302) with SH2D1B, PTPN6/SHP-1, PTPN11/SHP-2, INPP5D/SHIP1, CSK and FYN.1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ8BHK6, 6 interactors
STRINGi10090.ENSMUSP00000106907

Structurei

3D structure databases

ProteinModelPortaliQ8BHK6
SMRiQ8BHK6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 112Ig-like V-typeAdd BLAST86
Domaini128 – 203Ig-like C2-typeAdd BLAST76

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni276 – 294Interaction with FYN when phosphorylated at Tyr-282Add BLAST19

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi300 – 305ITSMBy similarity1 Publication6

Domaini

The ITSMs (immunoreceptor tyrosine-based switch motifs) with the consensus sequence T-X-Y-X-X-[VI] present in SLAM family receptors have overlapping specificity for activating and inhibitory SH2 domain-containing binding partners. Especially they mediate the interaction with the SH2 domain of SH2D1A and SH2D1B. A 'three-pronged' mechanism is proposed involving threonine (position -2), phosphorylated tyrosine (position 0) and valine/isoleucine (position +3).By similarity

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IJW6 Eukaryota
ENOG41117FX LUCA
HOGENOMiHOG000013065
HOVERGENiHBG079189
InParanoidiQ8BHK6
KOiK06733
PhylomeDBiQ8BHK6
TreeFamiTF334964

Family and domain databases

Gene3Di2.60.40.10, 2 hits
InterProiView protein in InterPro
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
SUPFAMiSSF48726 SSF48726, 1 hit
PROSITEiView protein in PROSITE
PS50835 IG_LIKE, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8BHK6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MARFSTYIIF TSVLCQLTVT AASGTLKKVA GALDGSVTFT LNITEIKVDY
60 70 80 90 100
VVWTFNTFFL AMVKKDGVTS QSSNKERIVF PDGLYSMKLS QLKKNDSGAY
110 120 130 140 150
RAEIYSTSSQ ASLIQEYVLH VYKHLSRPKV TIDRQSNKNG TCVINLTCST
160 170 180 190 200
DQDGENVTYS WKAVGQGDNQ FHDGATLSIA WRSGEKDQAL TCMARNPVSN
210 220 230 240 250
SFSTPVFPQK LCEDAATDLT SLRGILYILC FSAVLILFAV LLTIFHTTWI
260 270 280 290 300
KKGKGCEEDK KRVDRHQEMP DLCPHLEENA DYDTIPYTEK RRPEEDAPNT
310 320 330
FYSTVQIPKV VKSPSSLPAK PLVPRSLSFE NVI
Length:333
Mass (Da):37,187
Last modified:May 24, 2005 - v2
Checksum:i8D40B823FEBE7129
GO
Isoform 2 (identifier: Q8BHK6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     312-333: KSPSSLPAKPLVPRSLSFENVI → RSCPAEHHLTCQPLSLDHARAQ

Show »
Length:333
Mass (Da):37,291
Checksum:iC1A74D208A1106AA
GO
Isoform 3 (identifier: Q8BHK6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     255-289: Missing.
     312-333: KSPSSLPAKPLVPRSLSFENVI → RSCPAEHHLTCQPLSLDHARAQ

Show »
Length:298
Mass (Da):33,132
Checksum:i808710BEBC3DA336
GO
Isoform 4 (identifier: Q8BHK6-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     255-289: Missing.

Note: No experimental confirmation available.
Show »
Length:298
Mass (Da):33,028
Checksum:iCC60E5BDC892D4B5
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0R4J2D8A0A0R4J2D8_MOUSE
SLAM family member 7
Slamf7 mCG_10013
333Annotation score:
A0A0A6YW73A0A0A6YW73_MOUSE
SLAM family member 7
Slamf7 mCG_10013
294Annotation score:
A0A0R4J2D6A0A0R4J2D6_MOUSE
SLAM family member 7
Slamf7
335Annotation score:
A0A0A6YWL9A0A0A6YWL9_MOUSE
SLAM family member 7
Slamf7
281Annotation score:

Sequence cautioni

The sequence AAH11154 differs from that shown. Reason: Frameshift at position 255.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti118V → A in AAN63158 (PubMed:12242590).Curated1
Sequence conflicti248T → M in AAN63158 (PubMed:12242590).Curated1
Sequence conflicti248T → M in BAC26801 (PubMed:16141072).Curated1
Sequence conflicti248T → M in BAC26810 (PubMed:16141072).Curated1
Sequence conflicti248T → M in BAC30665 (PubMed:16141072).Curated1
Sequence conflicti248T → M in BAC40914 (PubMed:16141072).Curated1
Sequence conflicti253G → R in AAN63159 (PubMed:12242590).Curated1
Sequence conflicti253G → R (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_013783255 – 289Missing in isoform 3 and isoform 4. 2 PublicationsAdd BLAST35
Alternative sequenceiVSP_013784312 – 333KSPSS…FENVI → RSCPAEHHLTCQPLSLDHAR AQ in isoform 2 and isoform 3. 2 PublicationsAdd BLAST22

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF467909 mRNA Translation: AAN63158.1
AF467910 mRNA Translation: AAN63159.1
AF467911 mRNA Translation: AAN63160.1
AK030135 mRNA Translation: BAC26801.1
AK030148 mRNA Translation: BAC26810.1
AK040678 mRNA Translation: BAC30665.1
AK089525 mRNA Translation: BAC40914.1
BC011154 mRNA Translation: AAH11154.1 Frameshift.
CCDSiCCDS15500.1 [Q8BHK6-1]
RefSeqiNP_653122.2, NM_144539.5
XP_011237177.1, XM_011238875.2
XP_011237178.1, XM_011238876.2
UniGeneiMm.164642

Genome annotation databases

GeneIDi75345
KEGGimmu:75345
UCSCiuc007dov.1 mouse [Q8BHK6-1]
uc007doy.1 mouse [Q8BHK6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF467909 mRNA Translation: AAN63158.1
AF467910 mRNA Translation: AAN63159.1
AF467911 mRNA Translation: AAN63160.1
AK030135 mRNA Translation: BAC26801.1
AK030148 mRNA Translation: BAC26810.1
AK040678 mRNA Translation: BAC30665.1
AK089525 mRNA Translation: BAC40914.1
BC011154 mRNA Translation: AAH11154.1 Frameshift.
CCDSiCCDS15500.1 [Q8BHK6-1]
RefSeqiNP_653122.2, NM_144539.5
XP_011237177.1, XM_011238875.2
XP_011237178.1, XM_011238876.2
UniGeneiMm.164642

3D structure databases

ProteinModelPortaliQ8BHK6
SMRiQ8BHK6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BHK6, 6 interactors
STRINGi10090.ENSMUSP00000106907

PTM databases

iPTMnetiQ8BHK6
PhosphoSitePlusiQ8BHK6

Proteomic databases

EPDiQ8BHK6
PaxDbiQ8BHK6
PRIDEiQ8BHK6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi75345
KEGGimmu:75345
UCSCiuc007dov.1 mouse [Q8BHK6-1]
uc007doy.1 mouse [Q8BHK6-2]

Organism-specific databases

CTDi57823
MGIiMGI:1922595 Slamf7

Phylogenomic databases

eggNOGiENOG410IJW6 Eukaryota
ENOG41117FX LUCA
HOGENOMiHOG000013065
HOVERGENiHBG079189
InParanoidiQ8BHK6
KOiK06733
PhylomeDBiQ8BHK6
TreeFamiTF334964

Miscellaneous databases

PROiPR:Q8BHK6
SOURCEiSearch...

Gene expression databases

CleanExiMM_SLAMF7

Family and domain databases

Gene3Di2.60.40.10, 2 hits
InterProiView protein in InterPro
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
SUPFAMiSSF48726 SSF48726, 1 hit
PROSITEiView protein in PROSITE
PS50835 IG_LIKE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSLAF7_MOUSE
AccessioniPrimary (citable) accession number: Q8BHK6
Secondary accession number(s): Q8BTL2
, Q8CJ63, Q8CJ64, Q8CJ65, Q91XA0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 24, 2005
Last sequence update: May 24, 2005
Last modified: November 7, 2018
This is version 125 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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