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Protein

Proton-coupled amino acid transporter 2

Gene

Slc36a2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in a pH-dependent electrogenic neuronal transport and sequestration of small amino acids amino acids such as glycine, alanine and proline. Inhibited by sarcosine.3 Publications

Kineticsi

  1. KM=0.12 mM for proline1 Publication
  2. KM=6.5 mM for alanine1 Publication
  3. KM=30.9 mM for GABA1 Publication

    GO - Molecular functioni

    GO - Biological processi

    Enzyme and pathway databases

    ReactomeiR-MMU-352230 Amino acid transport across the plasma membrane
    R-MMU-428559 Proton-coupled neutral amino acid transporters

    Protein family/group databases

    TCDBi2.A.18.8.2 the amino acid/auxin permease (aaap) family

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proton-coupled amino acid transporter 2
    Short name:
    Proton/amino acid transporter 2
    Alternative name(s):
    Solute carrier family 36 member 2
    Tramdorin-1
    Gene namesi
    Name:Slc36a2
    Synonyms:Pat2, Tramd1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
    Proteomesi
    • UP000000589 Componenti: Chromosome 11

    Organism-specific databases

    MGIiMGI:1891430 Slc36a2

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 53CytoplasmicSequence analysisAdd BLAST53
    Transmembranei54 – 74HelicalSequence analysisAdd BLAST21
    Topological domaini75 – 76ExtracellularSequence analysis2
    Transmembranei77 – 97HelicalSequence analysisAdd BLAST21
    Topological domaini98 – 143CytoplasmicSequence analysisAdd BLAST46
    Transmembranei144 – 164HelicalSequence analysisAdd BLAST21
    Topological domaini165 – 192ExtracellularSequence analysisAdd BLAST28
    Transmembranei193 – 213HelicalSequence analysisAdd BLAST21
    Topological domaini214 – 217CytoplasmicSequence analysis4
    Transmembranei218 – 238HelicalSequence analysisAdd BLAST21
    Topological domaini239 – 259ExtracellularSequence analysisAdd BLAST21
    Transmembranei260 – 280HelicalSequence analysisAdd BLAST21
    Topological domaini281 – 292CytoplasmicSequence analysisAdd BLAST12
    Transmembranei293 – 313HelicalSequence analysisAdd BLAST21
    Topological domaini314 – 340ExtracellularSequence analysisAdd BLAST27
    Transmembranei341 – 361HelicalSequence analysisAdd BLAST21
    Topological domaini362 – 374CytoplasmicSequence analysisAdd BLAST13
    Transmembranei375 – 395HelicalSequence analysisAdd BLAST21
    Topological domaini396 – 399ExtracellularSequence analysis4
    Transmembranei400 – 420HelicalSequence analysisAdd BLAST21
    Topological domaini421 – 441CytoplasmicSequence analysisAdd BLAST21
    Transmembranei442 – 462HelicalSequence analysisAdd BLAST21
    Topological domaini463 – 478ExtracellularSequence analysisAdd BLAST16

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003248201 – 478Proton-coupled amino acid transporter 2Add BLAST478

    Proteomic databases

    PaxDbiQ8BHK3
    PRIDEiQ8BHK3

    PTM databases

    iPTMnetiQ8BHK3
    PhosphoSitePlusiQ8BHK3

    Expressioni

    Tissue specificityi

    Expressed in spinal cord, brain, testis, lung, heart, colon, spleen, kidney and muscle. Found in neuronal cell bodies in the anterior horn, in spinal cord brain stem, cerebellum, hippocampus, hypothalamus, rhinencephalon, cerebral cortex, and olfactory bulb in the brain. Also expressed in bone and fat tissues.3 Publications

    Gene expression databases

    BgeeiENSMUSG00000020264 Expressed in 110 organ(s), highest expression level in brown adipose tissue
    GenevisibleiQ8BHK3 MM

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000045613

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BHK3
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1304 Eukaryota
    COG0814 LUCA
    GeneTreeiENSGT00390000011245
    HOGENOMiHOG000006823
    HOVERGENiHBG057183
    InParanoidiQ8BHK3
    KOiK14209
    OMAiVGILCTY
    OrthoDBiEOG091G079T
    PhylomeDBiQ8BHK3
    TreeFamiTF314873

    Family and domain databases

    InterProiView protein in InterPro
    IPR013057 AA_transpt_TM
    PfamiView protein in Pfam
    PF01490 Aa_trans, 1 hit

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
    Isoform 1 (identifier: Q8BHK3-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide
            10         20         30         40         50
    MSVTKSARSP QVATPLNLDL PESAKKLQSQ DPSPANGSSS ESSKKTKGIT
    60 70 80 90 100
    GFQTLVHLVK GNMGTGILGL PLAVKNAGIL MGPLSLLVMG LIACHCMHIL
    110 120 130 140 150
    VRCAQRFCHR LNKPFMDYGD TVMHGLAFSP NAWLQNHAHW GRRVVSFFLI
    160 170 180 190 200
    VTQLGFCCVY IVFLADNLKQ VVEAVNSTTI SCHKNETVVL TPTMDSRLYM
    210 220 230 240 250
    LSFLPVLGLL VFVRNLRVLT IFSLLANISM LVSLVIIAQY IIQEIPDASQ
    260 270 280 290 300
    LPLVASWKTY PLFFGTAIFS FESIGVVLPL ENKMKDARGF PTILSLGMSI
    310 320 330 340 350
    ITTLYIAIGA LGYLRFGDDI KASITLNLPN CWLYQSVKLL YVVGILCTYA
    360 370 380 390 400
    LQFYVPAEII IPLAVSQVSK RWALPVDLSI RLALVCLTCM LAILIPRLDL
    410 420 430 440 450
    VLSLVGSVSS SALALIIPPL LEVVTYYGEG ISPLTVTKDA LISILGFMGF
    460 470
    VVGTYQALDE LIKSGNSPAL SNSTMFIQ
    Length:478
    Mass (Da):52,063
    Last modified:March 1, 2003 - v1
    Checksum:i0D4FE1C90F613A8A
    GO
    Isoform 2 (identifier: Q8BHK3-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         278-280: LPL → RFE
         281-478: Missing.

    Note: No experimental confirmation available.
    Show »
    Length:280
    Mass (Da):30,770
    Checksum:i331F03052C61FBF2
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti38S → T in AAM80481 (PubMed:11959859).Curated1
    Sequence conflicti38S → T in AAM44854 (PubMed:12451123).Curated1
    Sequence conflicti189V → A in AAM80481 (PubMed:11959859).Curated1
    Sequence conflicti189V → A in AAM44854 (PubMed:12451123).Curated1

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_032374278 – 280LPL → RFE in isoform 2. 1 Publication3
    Alternative sequenceiVSP_032375281 – 478Missing in isoform 2. 1 PublicationAdd BLAST198

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF453744 mRNA Translation: AAM80481.1
    AF512429 mRNA Translation: AAM44854.1
    AK041041 mRNA Translation: BAC30795.1
    AK079053 mRNA Translation: BAC37515.1
    AK086373 mRNA Translation: BAC39656.1
    AK156409 mRNA Translation: BAE33702.1
    AK160394 mRNA Translation: BAE35764.1
    AL713870 Genomic DNA No translation available.
    BC044800 mRNA Translation: AAH44800.1
    CCDSiCCDS24709.1 [Q8BHK3-1]
    RefSeqiNP_694810.2, NM_153170.3 [Q8BHK3-1]
    UniGeneiMm.291280

    Genome annotation databases

    EnsembliENSMUST00000039305; ENSMUSP00000045613; ENSMUSG00000020264 [Q8BHK3-1]
    GeneIDi246049
    KEGGimmu:246049
    UCSCiuc007iyz.2 mouse [Q8BHK3-1]
    uc007iza.1 mouse [Q8BHK3-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF453744 mRNA Translation: AAM80481.1
    AF512429 mRNA Translation: AAM44854.1
    AK041041 mRNA Translation: BAC30795.1
    AK079053 mRNA Translation: BAC37515.1
    AK086373 mRNA Translation: BAC39656.1
    AK156409 mRNA Translation: BAE33702.1
    AK160394 mRNA Translation: BAE35764.1
    AL713870 Genomic DNA No translation available.
    BC044800 mRNA Translation: AAH44800.1
    CCDSiCCDS24709.1 [Q8BHK3-1]
    RefSeqiNP_694810.2, NM_153170.3 [Q8BHK3-1]
    UniGeneiMm.291280

    3D structure databases

    ProteinModelPortaliQ8BHK3
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000045613

    Protein family/group databases

    TCDBi2.A.18.8.2 the amino acid/auxin permease (aaap) family

    PTM databases

    iPTMnetiQ8BHK3
    PhosphoSitePlusiQ8BHK3

    Proteomic databases

    PaxDbiQ8BHK3
    PRIDEiQ8BHK3

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSMUST00000039305; ENSMUSP00000045613; ENSMUSG00000020264 [Q8BHK3-1]
    GeneIDi246049
    KEGGimmu:246049
    UCSCiuc007iyz.2 mouse [Q8BHK3-1]
    uc007iza.1 mouse [Q8BHK3-2]

    Organism-specific databases

    CTDi153201
    MGIiMGI:1891430 Slc36a2

    Phylogenomic databases

    eggNOGiKOG1304 Eukaryota
    COG0814 LUCA
    GeneTreeiENSGT00390000011245
    HOGENOMiHOG000006823
    HOVERGENiHBG057183
    InParanoidiQ8BHK3
    KOiK14209
    OMAiVGILCTY
    OrthoDBiEOG091G079T
    PhylomeDBiQ8BHK3
    TreeFamiTF314873

    Enzyme and pathway databases

    ReactomeiR-MMU-352230 Amino acid transport across the plasma membrane
    R-MMU-428559 Proton-coupled neutral amino acid transporters

    Miscellaneous databases

    PROiPR:Q8BHK3
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSMUSG00000020264 Expressed in 110 organ(s), highest expression level in brown adipose tissue
    GenevisibleiQ8BHK3 MM

    Family and domain databases

    InterProiView protein in InterPro
    IPR013057 AA_transpt_TM
    PfamiView protein in Pfam
    PF01490 Aa_trans, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiS36A2_MOUSE
    AccessioniPrimary (citable) accession number: Q8BHK3
    Secondary accession number(s): Q8BUB0, Q8JZP1
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: March 1, 2003
    Last modified: September 12, 2018
    This is version 118 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health

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