UniProtKB - Q8BHG1 (NRDC_MOUSE)
Protein
Nardilysin
Gene
Nrdc
Organism
Mus musculus (Mouse)
Status
Functioni
Cleaves peptide substrates on the N-terminus of arginine residues in dibasic pairs.
Catalytic activityi
- Hydrolysis of polypeptides, preferably at -Xaa-|-Arg-Lys-, and less commonly at -Arg-|-Arg-Xaa-, in which Xaa is not Arg or Lys. EC:3.4.24.61
Cofactori
Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 244 | ZincPROSITE-ProRule annotation | 1 | |
Active sitei | 247 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Metal bindingi | 248 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 325 | ZincPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- metalloendopeptidase activity Source: UniProtKB-EC
- peptidase activity Source: MGI
GO - Biological processi
- negative regulation of cold-induced thermogenesis Source: YuBioLab
- positive regulation of membrane protein ectodomain proteolysis Source: MGI
- regulation of endopeptidase activity Source: MGI
Keywordsi
Molecular function | Hydrolase, Metalloprotease, Protease |
Ligand | Metal-binding, Zinc |
Protein family/group databases
MEROPSi | M16.005 |
Names & Taxonomyi
Protein namesi | Recommended name: Nardilysin (EC:3.4.24.61)Alternative name(s): N-arginine dibasic convertase Short name: NRD convertase Short name: NRD-C Nardilysin convertaseBy similarity |
Gene namesi | Name:NrdcBy similarity Synonyms:Nrd1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1201386, Nrd1 |
Subcellular locationi
Mitochondrion
- mitochondrion Source: MGI
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 18 | Sequence analysisAdd BLAST | 18 | |
ChainiPRO_0000026756 | 19 – 1161 | NardilysinAdd BLAST | 1143 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 85 | PhosphoserineCombined sources | 1 | |
Modified residuei | 91 | PhosphoserineBy similarity | 1 | |
Modified residuei | 93 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q8BHG1 |
jPOSTi | Q8BHG1 |
MaxQBi | Q8BHG1 |
PaxDbi | Q8BHG1 |
PRIDEi | Q8BHG1 |
PTM databases
iPTMneti | Q8BHG1 |
PhosphoSitePlusi | Q8BHG1 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000053510, Expressed in testis and 309 other tissues |
ExpressionAtlasi | Q8BHG1, baseline and differential |
Genevisiblei | Q8BHG1, MM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 230982, 12 interactors |
IntActi | Q8BHG1, 1 interactor |
MINTi | Q8BHG1 |
STRINGi | 10090.ENSMUSP00000068328 |
Miscellaneous databases
RNActi | Q8BHG1, protein |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 139 – 209 | Asp/Glu-rich (highly acidic)Add BLAST | 71 | |
Compositional biasi | 140 – 151 | Poly-GluAdd BLAST | 12 | |
Compositional biasi | 152 – 165 | Poly-AspAdd BLAST | 14 | |
Compositional biasi | 185 – 190 | Poly-Asp | 6 | |
Compositional biasi | 193 – 196 | Poly-Asp | 4 |
Sequence similaritiesi
Belongs to the peptidase M16 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG0959, Eukaryota |
GeneTreei | ENSGT00940000155026 |
HOGENOMi | CLU_004639_1_0_1 |
InParanoidi | Q8BHG1 |
OMAi | FAKFSVG |
PhylomeDBi | Q8BHG1 |
TreeFami | TF106274 |
Family and domain databases
InterProi | View protein in InterPro IPR011249, Metalloenz_LuxS/M16 IPR011765, Pept_M16_N IPR001431, Pept_M16_Zn_BS IPR007863, Peptidase_M16_C IPR032632, Peptidase_M16_M |
Pfami | View protein in Pfam PF00675, Peptidase_M16, 1 hit PF05193, Peptidase_M16_C, 2 hits PF16187, Peptidase_M16_M, 1 hit |
SUPFAMi | SSF63411, SSF63411, 4 hits |
PROSITEi | View protein in PROSITE PS00143, INSULINASE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
Q8BHG1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLRRVAVAAV CVTGRKLRCE AGRELTALGR IEARGLCEES SKPFPTLTMP
60 70 80 90 100
GRNKAKSTCS CPDLQPNGQD LGESGRLARL GADESEEEGR SFSNVGDPEI
110 120 130 140 150
IKSPSDPKQY RYIKLQNGLQ ALLISDLSNV EGKTGNATDE EEEEEEEEEE
160 170 180 190 200
EDDDDDDDDD DDDEDSGAEI QDDDEEGFDD EEEFDDDDDD EHDDDDLENE
210 220 230 240 250
ENELEELEER VEARKKTTEK QSAAALCVGV GSFADPDDLP GLAHFLEHMV
260 270 280 290 300
FMGSLKYPDE NGFDAFLKKH GGSDNASTDC ERTVFQFDVQ RKYFKEALDR
310 320 330 340 350
WAQFFIHPLM IRDAIDREVE AVDSEYQLAR PSDANRKEML FGSLARPGHP
360 370 380 390 400
MGKFFWGNAE TLKHEPKKNN IDTHARLREF WMRYYSAHYM TLVVQSKETL
410 420 430 440 450
DTLEKWVTEI FSQIPNNGLP KPNFSHLTDP FDTPAFNKLY RVVPIRKIHA
460 470 480 490 500
LTITWALPPQ QQHYRVKPLH YISWLVGHEG KGSILSYLRK KCWALALFGG
510 520 530 540 550
NGETGFEQNS TYSVFSISIT LTDEGYEHFY EVAHTVFQYL KMLQKLGPEK
560 570 580 590 600
RVFEEIQKIE DNEFHYQEQT DPVEYVENMC ENMQLYPRQD FLTGDQLLFE
610 620 630 640 650
YKPEVIAEAL NQLVPQKANL VLLSGANEGR CDLKEKWFGT QYSIEDIENS
660 670 680 690 700
WTELWKSNFD LNPDLHLPAE NKYIATDFTL KAFDCPETEY PAKIVNTAQG
710 720 730 740 750
CLWYKKDNKF KIPKAYIRFH LISPLIQKSA ANVVLFDIFV NILTHNLAEP
760 770 780 790 800
AYEADVAQLE YKLVAGEHGL IIRVKGFNHK LPLLFQLIID YLTEFSSTPA
810 820 830 840 850
VFTMITEQLK KTYFNILIKP ETLAKDVRLL ILEYSRWSMI DKYQALMDGL
860 870 880 890 900
SLDSLLNFVK DFKSQLFVEG LVQGNVTSTE SMDFLKYVVD KLNFAPLERE
910 920 930 940 950
MPVQFQVVEL PSGHHLCKVR ALNKGDANSE VTVYYQSGTR SLREYTLMEL
960 970 980 990 1000
LVMHMEEPCF DFLRTKQTLG YHVYPTCRNT SGILGFSVTV GTQATKYNSE
1010 1020 1030 1040 1050
TVDKKIEEFL SSFEEKIENL TEDAFNTQVT ALIKLKECED THLGEEVDRN
1060 1070 1080 1090 1100
WNEVVTQQYL FDRLAHEIEA LKSFSKSDLV SWFKAHRGPG SKMLSVHVVG
1110 1120 1130 1140 1150
YGKYELEEDG APFGEDSNSR EGMQLTYLPP SPVLAESTTP ITDIRAFTAT
1160
LSLFPYHKIV K
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA2A9Q2 | A2A9Q2_MOUSE | Nardilysin, N-arginine dibasic conv... | Nrd1 | 1,229 | Annotation score: | ||
A6PWC3 | A6PWC3_MOUSE | Nardilysin, N-arginine dibasic conv... | Nrd1 | 1,117 | Annotation score: | ||
Q3V3G9 | Q3V3G9_MOUSE | Nardilysin, N-arginine dibasic conv... | Nrd1 | 395 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK031548 mRNA Translation: BAC27445.1 BC036128 mRNA Translation: AAH36128.1 |
CCDSi | CCDS18460.1 |
RefSeqi | NP_666262.2, NM_146150.2 |
Genome annotation databases
Ensembli | ENSMUST00000065977; ENSMUSP00000068328; ENSMUSG00000053510 |
GeneIDi | 230598 |
KEGGi | mmu:230598 |
UCSCi | uc008ubw.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK031548 mRNA Translation: BAC27445.1 BC036128 mRNA Translation: AAH36128.1 |
CCDSi | CCDS18460.1 |
RefSeqi | NP_666262.2, NM_146150.2 |
3D structure databases
SMRi | Q8BHG1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 230982, 12 interactors |
IntActi | Q8BHG1, 1 interactor |
MINTi | Q8BHG1 |
STRINGi | 10090.ENSMUSP00000068328 |
Protein family/group databases
MEROPSi | M16.005 |
PTM databases
iPTMneti | Q8BHG1 |
PhosphoSitePlusi | Q8BHG1 |
Proteomic databases
EPDi | Q8BHG1 |
jPOSTi | Q8BHG1 |
MaxQBi | Q8BHG1 |
PaxDbi | Q8BHG1 |
PRIDEi | Q8BHG1 |
Protocols and materials databases
Antibodypediai | 19043, 140 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000065977; ENSMUSP00000068328; ENSMUSG00000053510 |
GeneIDi | 230598 |
KEGGi | mmu:230598 |
UCSCi | uc008ubw.1, mouse |
Organism-specific databases
CTDi | 230598 |
MGIi | MGI:1201386, Nrd1 |
Phylogenomic databases
eggNOGi | KOG0959, Eukaryota |
GeneTreei | ENSGT00940000155026 |
HOGENOMi | CLU_004639_1_0_1 |
InParanoidi | Q8BHG1 |
OMAi | FAKFSVG |
PhylomeDBi | Q8BHG1 |
TreeFami | TF106274 |
Miscellaneous databases
BioGRID-ORCSi | 230598, 5 hits in 19 CRISPR screens |
ChiTaRSi | Nrd1, mouse |
PROi | PR:Q8BHG1 |
RNActi | Q8BHG1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000053510, Expressed in testis and 309 other tissues |
ExpressionAtlasi | Q8BHG1, baseline and differential |
Genevisiblei | Q8BHG1, MM |
Family and domain databases
InterProi | View protein in InterPro IPR011249, Metalloenz_LuxS/M16 IPR011765, Pept_M16_N IPR001431, Pept_M16_Zn_BS IPR007863, Peptidase_M16_C IPR032632, Peptidase_M16_M |
Pfami | View protein in Pfam PF00675, Peptidase_M16, 1 hit PF05193, Peptidase_M16_C, 2 hits PF16187, Peptidase_M16_M, 1 hit |
SUPFAMi | SSF63411, SSF63411, 4 hits |
PROSITEi | View protein in PROSITE PS00143, INSULINASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NRDC_MOUSE | |
Accessioni | Q8BHG1Primary (citable) accession number: Q8BHG1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 11, 2003 |
Last sequence update: | March 1, 2003 | |
Last modified: | December 2, 2020 | |
This is version 142 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Peptidase families
Classification of peptidase families and list of entries