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Protein

Gem-associated protein 8

Gene

Gemin8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus (By similarity).By similarity

GO - Biological processi

Keywordsi

Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

ReactomeiR-MMU-191859 snRNP Assembly

Names & Taxonomyi

Protein namesi
Recommended name:
Gem-associated protein 8
Short name:
Gemin-8
Alternative name(s):
Protein FAM51A1
Gene namesi
Name:Gemin8
Synonyms:Fam51a1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:2384300 Gemin8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000871561 – 238Gem-associated protein 8Add BLAST238

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei122PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8BHE1
MaxQBiQ8BHE1
PaxDbiQ8BHE1
PeptideAtlasiQ8BHE1
PRIDEiQ8BHE1

PTM databases

iPTMnetiQ8BHE1
PhosphoSitePlusiQ8BHE1

Expressioni

Tissue specificityi

Widely expressed in embryonic tissues (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000040621 Expressed in 72 organ(s), highest expression level in thymus
CleanExiMM_GEMIN8
ExpressionAtlasiQ8BHE1 baseline and differential
GenevisibleiQ8BHE1 MM

Interactioni

Subunit structurei

Part of the core SMN complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8 and STRAP/UNRIP. Part of the SMN-Sm complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8, STRAP/UNRIP and the Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG. Interacts directly with SMN1 and with the GEMIN6-GEMIN7 heterodimer, mediating their interaction (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000084414

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili131 – 164Sequence analysisAdd BLAST34

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IWPF Eukaryota
ENOG4111HJ5 LUCA
GeneTreeiENSGT00390000013608
HOGENOMiHOG000049212
HOVERGENiHBG051527
InParanoidiQ8BHE1
KOiK13136
OMAiMAWMRSH
OrthoDBiEOG091G0H89
PhylomeDBiQ8BHE1
TreeFamiTF328591

Family and domain databases

InterProiView protein in InterPro
IPR034754 GEMIN8
PANTHERiPTHR16238 PTHR16238, 1 hit
PfamiView protein in Pfam
PF15348 GEMIN8, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q8BHE1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASNWRASAS WYSHPVYARY WQHYHHAMLW MQGHQNAYRK FRDSYFTSPW
60 70 80 90 100
LFPHGALPWN SPAYEAGHPW DSQGQHMAQQ ESPYRVSHPK SPGQPLRNSS
110 120 130 140 150
RTQASTRGNE ARCEEEELES DSDDEVECDL SNMEITEELR QYFAQTERHR
160 170 180 190 200
EERRRQQQLD AERLNDYVNA DHGLYFNHRR SLEPPSEKPW ERRQAEMKRL
210 220 230
YGNSAPKILA METAVQLSFD KHCDRKQPKY WPVIPLKF
Length:238
Mass (Da):28,396
Last modified:March 1, 2003 - v1
Checksum:i2C07078598BB4531
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B1AVP5B1AVP5_MOUSE
Gem-associated protein 8
Gemin8
207Annotation score:
E9PZ44E9PZ44_MOUSE
Gem-associated protein 8
Gemin8
186Annotation score:
E9QA54E9QA54_MOUSE
Gem-associated protein 8
Gemin8
147Annotation score:

Sequence cautioni

The sequence AAH23488 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti97R → H in AAH23488 (PubMed:15489334).Curated1
Sequence conflicti166D → Y in AAH23488 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK047511 mRNA Translation: BAC33077.1
AK053774 mRNA Translation: BAC35516.1
AK160583 mRNA Translation: BAE35890.1
AL713990 Genomic DNA No translation available.
BC023488 mRNA Translation: AAH23488.1 Different initiation.
CCDSiCCDS41207.1
RefSeqiNP_001297651.1, NM_001310722.1
NP_001297653.1, NM_001310724.1
NP_666350.2, NM_146238.4
UniGeneiMm.209490

Genome annotation databases

EnsembliENSMUST00000056410; ENSMUSP00000085137; ENSMUSG00000040621
ENSMUST00000087169; ENSMUSP00000084414; ENSMUSG00000040621
ENSMUST00000130880; ENSMUSP00000118482; ENSMUSG00000040621
GeneIDi237221
KEGGimmu:237221
UCSCiuc009uwc.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK047511 mRNA Translation: BAC33077.1
AK053774 mRNA Translation: BAC35516.1
AK160583 mRNA Translation: BAE35890.1
AL713990 Genomic DNA No translation available.
BC023488 mRNA Translation: AAH23488.1 Different initiation.
CCDSiCCDS41207.1
RefSeqiNP_001297651.1, NM_001310722.1
NP_001297653.1, NM_001310724.1
NP_666350.2, NM_146238.4
UniGeneiMm.209490

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000084414

PTM databases

iPTMnetiQ8BHE1
PhosphoSitePlusiQ8BHE1

Proteomic databases

EPDiQ8BHE1
MaxQBiQ8BHE1
PaxDbiQ8BHE1
PeptideAtlasiQ8BHE1
PRIDEiQ8BHE1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000056410; ENSMUSP00000085137; ENSMUSG00000040621
ENSMUST00000087169; ENSMUSP00000084414; ENSMUSG00000040621
ENSMUST00000130880; ENSMUSP00000118482; ENSMUSG00000040621
GeneIDi237221
KEGGimmu:237221
UCSCiuc009uwc.1 mouse

Organism-specific databases

CTDi54960
MGIiMGI:2384300 Gemin8

Phylogenomic databases

eggNOGiENOG410IWPF Eukaryota
ENOG4111HJ5 LUCA
GeneTreeiENSGT00390000013608
HOGENOMiHOG000049212
HOVERGENiHBG051527
InParanoidiQ8BHE1
KOiK13136
OMAiMAWMRSH
OrthoDBiEOG091G0H89
PhylomeDBiQ8BHE1
TreeFamiTF328591

Enzyme and pathway databases

ReactomeiR-MMU-191859 snRNP Assembly

Miscellaneous databases

PROiPR:Q8BHE1
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040621 Expressed in 72 organ(s), highest expression level in thymus
CleanExiMM_GEMIN8
ExpressionAtlasiQ8BHE1 baseline and differential
GenevisibleiQ8BHE1 MM

Family and domain databases

InterProiView protein in InterPro
IPR034754 GEMIN8
PANTHERiPTHR16238 PTHR16238, 1 hit
PfamiView protein in Pfam
PF15348 GEMIN8, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGEMI8_MOUSE
AccessioniPrimary (citable) accession number: Q8BHE1
Secondary accession number(s): B1AVP4, Q3TUT1, Q8R3W9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: March 1, 2003
Last modified: September 12, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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