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Protein

E3 ubiquitin-protein ligase NRDP1

Gene

Rnf41

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as E3 ubiquitin-protein ligase and regulates the degradation of target proteins. Polyubiquitinates MYD88 (By similarity). Negatively regulates MYD88-dependent production of proinflammatory cytokines. Can promote TRIF-dependent production of type I interferon and inhibits infection with vesicular stomatitis virus. Promotes also activation of TBK1 and IRF3 (PubMed:19483718). Involved in the ubiquitination of erythropoietin (EPO) and interleukin-3 (IL-3) receptors. Thus, through maintaining basal levels of cytokine receptors, RNF41 is involved in the control of hematopoietic progenitor cell differentiation into myeloerythroid lineages (PubMed:18495327). Contributes to the maintenance of steady-state ERBB3 levels by mediating its growth factor-independent degradation. Involved in the degradation of the inhibitor of apoptosis BIRC6 and thus is an important regulator of cell death by promoting apoptosis. Acts also as a PRKN modifier that accelerates its degradation, resulting in a reduction of PRKN activity, influencing the balance of intracellular redox state. The RNF41-PRKN pathway regulates autophagosome-lysosome fusion during late mitophagy. Mitophagy is a selective form of autophagy necessary for mitochondrial quality control (PubMed:24949970).By similarity3 Publications

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri18 – 57RING-type; degeneratePROSITE-ProRule annotationAdd BLAST40
Zinc fingeri78 – 138SIAH-type; degeneratePROSITE-ProRule annotationAdd BLAST61

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processApoptosis, Autophagy, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-1358803 Downregulation of ERBB2:ERBB3 signaling
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayi
UPA00143

Protein family/group databases

MoonDBiQ8BH75 Predicted

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase NRDP1 (EC:2.3.2.27)
Alternative name(s):
RING finger protein 41
RING-type E3 ubiquitin transferase NRDP1Curated
Gene namesi
Name:Rnf41
Synonyms:Flrf, Nrdp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1914838 Rnf41

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002239551 – 317E3 ubiquitin-protein ligase NRDP1Add BLAST317

Post-translational modificationi

Autoubiquitinated. Autoubiquitination leads to proteasomal degradation. Deubiquitinated by USP8 to get stabilized which induces apoptosis.By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ8BH75
PRIDEiQ8BH75

PTM databases

iPTMnetiQ8BH75
PhosphoSitePlusiQ8BH75

Expressioni

Gene expression databases

BgeeiENSMUSG00000025373 Expressed in 263 organ(s), highest expression level in embryo
CleanExiMM_RNF41
ExpressionAtlasiQ8BH75 baseline and differential
GenevisibleiQ8BH75 MM

Interactioni

Subunit structurei

Interacts with USP8, ERBB3, PRKN and BIRC6 (By similarity). Interacts with CSF2RB, EPOR, IL3RA, MYD88 and TBK1. Interacts with Clec16a (PubMed:24949970).By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Clec16aQ80U30-23EBI-7059583,EBI-9696757

GO - Molecular functioni

Protein-protein interaction databases

BioGridi212296, 27 interactors
IntActiQ8BH75, 3 interactors
MINTiQ8BH75
STRINGi10090.ENSMUSP00000100869

Structurei

Secondary structure

1317
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ8BH75
SMRiQ8BH75
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8BH75

Family & Domainsi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri18 – 57RING-type; degeneratePROSITE-ProRule annotationAdd BLAST40
Zinc fingeri78 – 138SIAH-type; degeneratePROSITE-ProRule annotationAdd BLAST61

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410ISDX Eukaryota
ENOG410XNN7 LUCA
GeneTreeiENSGT00530000063647
HOGENOMiHOG000006561
HOVERGENiHBG053154
InParanoidiQ8BH75
KOiK11981
OMAiSRQPTCP
OrthoDBiEOG091G0BKR
PhylomeDBiQ8BH75
TreeFamiTF351947

Family and domain databases

Gene3Di3.30.40.10, 2 hits
InterProiView protein in InterPro
IPR015036 NRDP1
IPR037255 NRDP1_C
IPR008974 TRAF-like
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
IPR013010 Znf_SIAH
PfamiView protein in Pfam
PF08941 USP8_interact, 1 hit
SMARTiView protein in SMART
SM00184 RING, 1 hit
SUPFAMiSSF160088 SSF160088, 1 hit
SSF49599 SSF49599, 1 hit
PROSITEiView protein in PROSITE
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit
PS51081 ZF_SIAH, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8BH75-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGYDVTRFQG DVDEDLICPI CSGVLEEPVQ APHCEHAFCN ACITQWFSQQ
60 70 80 90 100
QTCPVDRSVV TVAHLRPVPR IMRNMLSKLQ IACDNAVFGC SAVVRLDNLM
110 120 130 140 150
SHLSDCEHNP KRPVTCEQGC GLEMPKDELP NHNCIKHLRS VVQQQQSRIA
160 170 180 190 200
ELEKTSAEHK HQLAEQKRDI QLLKAYMRAI RSVNPNLQNL EETIEYNEIL
210 220 230 240 250
EWVNSLQPAR VTRWGGMIST PDAVLQAVIK RSLVESGCPA SIVNELIENA
260 270 280 290 300
HERSWPQGLA TLETRQMNRR YYENYVAKRI PGKQAVVVMA CENQHMGDDM
310
VQEPGLVMIF AHGVEEI
Length:317
Mass (Da):35,891
Last modified:March 1, 2003 - v1
Checksum:i43FE844C06392802
GO
Isoform 2 (identifier: Q8BH75-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     31-36: APHCEH → PGDAQR
     37-317: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:36
Mass (Da):3,953
Checksum:i09DBCB86853E88F0
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1W2P811A0A1W2P811_MOUSE
E3 ubiquitin-protein ligase NRDP1
Rnf41
122Annotation score:

Sequence cautioni

The sequence AAH19415 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAC27496 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.Curated
The sequence BAC31779 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7R → H in BAC31779 (PubMed:16141072).Curated1
Sequence conflicti104S → T in AAH19415 (PubMed:15489334).Curated1
Sequence conflicti156S → R in AAH19415 (PubMed:15489334).Curated1
Sequence conflicti178R → Q in BAC28054 (PubMed:16141072).Curated1
Sequence conflicti278K → E in BAB29953 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05864931 – 36APHCEH → PGDAQR in isoform 2. 1 Publication6
Alternative sequenceiVSP_05865037 – 317Missing in isoform 2. 1 PublicationAdd BLAST281

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK015745 mRNA Translation: BAB29953.1
AK020527 mRNA Translation: BAB32125.1
AK028551 mRNA Translation: BAC26004.1
AK030558 mRNA Translation: BAC27020.1
AK031657 mRNA Translation: BAC27496.1 Sequence problems.
AK032848 mRNA Translation: BAC28054.1
AK034551 mRNA Translation: BAC28750.1
AK044103 mRNA Translation: BAC31779.1 Sequence problems.
AK077466 mRNA Translation: BAC36813.1
BC019415 mRNA Translation: AAH19415.1 Different initiation.
BC049078 mRNA Translation: AAH49078.1
CCDSiCCDS24277.1 [Q8BH75-1]
RefSeqiNP_001157709.1, NM_001164237.1 [Q8BH75-1]
NP_080535.2, NM_026259.3 [Q8BH75-1]
UniGeneiMm.294120

Genome annotation databases

EnsembliENSMUST00000096386; ENSMUSP00000100869; ENSMUSG00000025373 [Q8BH75-1]
ENSMUST00000171342; ENSMUSP00000132751; ENSMUSG00000025373 [Q8BH75-1]
ENSMUST00000217826; ENSMUSP00000151260; ENSMUSG00000025373 [Q8BH75-2]
GeneIDi67588
KEGGimmu:67588
UCSCiuc007hmr.2 mouse [Q8BH75-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK015745 mRNA Translation: BAB29953.1
AK020527 mRNA Translation: BAB32125.1
AK028551 mRNA Translation: BAC26004.1
AK030558 mRNA Translation: BAC27020.1
AK031657 mRNA Translation: BAC27496.1 Sequence problems.
AK032848 mRNA Translation: BAC28054.1
AK034551 mRNA Translation: BAC28750.1
AK044103 mRNA Translation: BAC31779.1 Sequence problems.
AK077466 mRNA Translation: BAC36813.1
BC019415 mRNA Translation: AAH19415.1 Different initiation.
BC049078 mRNA Translation: AAH49078.1
CCDSiCCDS24277.1 [Q8BH75-1]
RefSeqiNP_001157709.1, NM_001164237.1 [Q8BH75-1]
NP_080535.2, NM_026259.3 [Q8BH75-1]
UniGeneiMm.294120

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2OGBX-ray1.95A/B193-317[»]
ProteinModelPortaliQ8BH75
SMRiQ8BH75
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212296, 27 interactors
IntActiQ8BH75, 3 interactors
MINTiQ8BH75
STRINGi10090.ENSMUSP00000100869

Protein family/group databases

MoonDBiQ8BH75 Predicted

PTM databases

iPTMnetiQ8BH75
PhosphoSitePlusiQ8BH75

Proteomic databases

PaxDbiQ8BH75
PRIDEiQ8BH75

Protocols and materials databases

DNASUi67588
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000096386; ENSMUSP00000100869; ENSMUSG00000025373 [Q8BH75-1]
ENSMUST00000171342; ENSMUSP00000132751; ENSMUSG00000025373 [Q8BH75-1]
ENSMUST00000217826; ENSMUSP00000151260; ENSMUSG00000025373 [Q8BH75-2]
GeneIDi67588
KEGGimmu:67588
UCSCiuc007hmr.2 mouse [Q8BH75-1]

Organism-specific databases

CTDi10193
MGIiMGI:1914838 Rnf41

Phylogenomic databases

eggNOGiENOG410ISDX Eukaryota
ENOG410XNN7 LUCA
GeneTreeiENSGT00530000063647
HOGENOMiHOG000006561
HOVERGENiHBG053154
InParanoidiQ8BH75
KOiK11981
OMAiSRQPTCP
OrthoDBiEOG091G0BKR
PhylomeDBiQ8BH75
TreeFamiTF351947

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-MMU-1358803 Downregulation of ERBB2:ERBB3 signaling
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

EvolutionaryTraceiQ8BH75
PROiPR:Q8BH75
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025373 Expressed in 263 organ(s), highest expression level in embryo
CleanExiMM_RNF41
ExpressionAtlasiQ8BH75 baseline and differential
GenevisibleiQ8BH75 MM

Family and domain databases

Gene3Di3.30.40.10, 2 hits
InterProiView protein in InterPro
IPR015036 NRDP1
IPR037255 NRDP1_C
IPR008974 TRAF-like
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
IPR013010 Znf_SIAH
PfamiView protein in Pfam
PF08941 USP8_interact, 1 hit
SMARTiView protein in SMART
SM00184 RING, 1 hit
SUPFAMiSSF160088 SSF160088, 1 hit
SSF49599 SSF49599, 1 hit
PROSITEiView protein in PROSITE
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit
PS51081 ZF_SIAH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRNF41_MOUSE
AccessioniPrimary (citable) accession number: Q8BH75
Secondary accession number(s): Q8BGJ2
, Q8BMC9, Q8VEA2, Q9CRK6, Q9D568
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: March 1, 2003
Last modified: September 12, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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