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Protein

Golgi-associated PDZ and coiled-coil motif-containing protein

Gene

Gopc

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in intracellular protein trafficking and degradation. May regulate CFTR chloride currents and acid-induced ASIC3 currents by modulating cell surface expression of both channels. May also regulate the intracellular trafficking of the ADR1B receptor. May play a role in autophagy. Overexpression results in CFTR intracellular retention and degradation in the lysosomes.3 Publications

GO - Molecular functioni

  • frizzled binding Source: BHF-UCL
  • GTPase regulator activity Source: MGI
  • ion channel binding Source: UniProtKB
  • protein C-terminus binding Source: BHF-UCL
  • protein homodimerization activity Source: BHF-UCL
  • syntaxin binding Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Golgi-associated PDZ and coiled-coil motif-containing protein
Alternative name(s):
PDZ protein interacting specifically with TC10
Short name:
PIST
Gene namesi
Name:Gopc
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:2149946 Gopc

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Golgi apparatus, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

Male mice are infertile with globozoospermia. Spermatozoa display a default in acrosome formation and are unable to activate oocytes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000875432 – 463Golgi-associated PDZ and coiled-coil motif-containing proteinAdd BLAST462

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei402PhosphoserineCombined sources1
Modified residuei405PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ8BH60
PRIDEiQ8BH60

PTM databases

iPTMnetiQ8BH60
PhosphoSitePlusiQ8BH60

Expressioni

Tissue specificityi

Ubiquitously expressed (at protein level). Expressed in dorsal root glanglion (DRG), spinal chord and brain. Isoform 1 is preferentially expressed in whole brain (at protein level) and cerebellum. Expressed in spermatocytes and spermatides but not in Sertoli cells and spermatogonia.4 Publications

Developmental stagei

In the cerebellum, expression increases post-natally, following maturation of this tissue (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000019861 Expressed in 273 organ(s), highest expression level in ureteric bud tip
ExpressionAtlasiQ8BH60 baseline and differential
GenevisibleiQ8BH60 MM

Interactioni

Subunit structurei

Homooligomer. Interacts with NLGN1, STX6 and GOLGA3 (By similarity). Interacts with ASIC3, RHOQ, FZD5, FZD8, GRID2, BECN1, CSPG5, CFTR and CLCN3. May interact with CACNG2.By similarity7 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi220478, 3 interactors
IntActiQ8BH60, 11 interactors
MINTiQ8BH60
STRINGi10090.ENSMUSP00000020008

Structurei

3D structure databases

ProteinModelPortaliQ8BH60
SMRiQ8BH60
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini289 – 372PDZPROSITE-ProRule annotationAdd BLAST84

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili85 – 201Sequence analysisAdd BLAST117

Domaini

The coiled-coil region probably mediates targeting to the Golgi, oligomerization and interaction with RHOQ. May also mediates association to membranes and interactions with GOLGA3 and STX6 (By similarity).By similarity
The PDZ domain mediates interaction with ADRB1 (By similarity). Mediates also interactions with FZD5, FZD8, ASIC3, GRID2, CFTR, CLCN3.By similarity3 Publications

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410ITAF Eukaryota
ENOG410XT8J LUCA
GeneTreeiENSGT00550000074581
HOGENOMiHOG000280727
HOVERGENiHBG057507
InParanoidiQ8BH60
PhylomeDBiQ8BH60

Family and domain databases

InterProiView protein in InterPro
IPR038879 GOPC
IPR001478 PDZ
IPR036034 PDZ_sf
PANTHERiPTHR16528 PTHR16528, 1 hit
PfamiView protein in Pfam
PF00595 PDZ, 1 hit
SMARTiView protein in SMART
SM00228 PDZ, 1 hit
SUPFAMiSSF50156 SSF50156, 1 hit
PROSITEiView protein in PROSITE
PS50106 PDZ, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8BH60-1) [UniParc]FASTAAdd to basket
Also known as: NPIST, Beta

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSAGGPCPAG AGGGPGGSSC PVGVSPGGVS MFRWLEVLEK EFDKAFVDVD
60 70 80 90 100
LLLGEIDPDQ ADITYEGRQK MTSLSSCFAQ LCHKAQTVSQ INHKLEAQLV
110 120 130 140 150
DLRSELTETQ AEKVVLEKEV HEQLLQLHST QLQLHAKTGQ SVDSGAIKAK
160 170 180 190 200
LSVHSVEDLE RELEANKTEK VKEARLEAEV KLLRKENEAL RRHIAVLQAE
210 220 230 240 250
VYGARLAAKY LDKELAGRVQ QIQLLGRDMK GPAHDKLWNQ LEAEIHLHRH
260 270 280 290 300
KTVIRACRGR NDLKRPMQAP PGHDQDSLKK SQGVGPIRKV LLLKEDHEGL
310 320 330 340 350
GISITGGKEH GVPILISEIH PGQPADRCGG LHVGDAILAV NGVNLRDTKH
360 370 380 390 400
KEAVTILSQQ RGEIEFEVVY VAPEVDSDDE NVEYEDESGH RYRLYLDELE
410 420 430 440 450
GSGNSGASCK DSSGEMKMLQ GYNKKAVRDA HENGDVGAAG ESPLDDTAAR
460
AAHLHSLHQK KAY
Length:463
Mass (Da):50,662
Last modified:March 1, 2003 - v1
Checksum:i47BB5760BFFB6303
GO
Isoform 2 (identifier: Q8BH60-2) [UniParc]FASTAAdd to basket
Also known as: Alpha

The sequence of this isoform differs from the canonical sequence as follows:
     151-158: Missing.

Show »
Length:455
Mass (Da):49,795
Checksum:iC75E9F6D2C243CFB
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
K3W4Q9K3W4Q9_MOUSE
Golgi-associated PDZ and coiled-coi...
Gopc
463Annotation score:
A0A1W2P7V0A0A1W2P7V0_MOUSE
Golgi-associated PDZ and coiled-coi...
Gopc
403Annotation score:
A0A1W2P7A5A0A1W2P7A5_MOUSE
Golgi-associated PDZ and coiled-coi...
Gopc
103Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_016065151 – 158Missing in isoform 2. 3 Publications8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF287893 mRNA Translation: AAG00571.1
AB052838 mRNA Translation: BAB69946.1
AK030427 mRNA Translation: BAC26958.1
AK030637 mRNA Translation: BAC27058.1
AK032613 mRNA Translation: BAC27951.1
BC051171 mRNA Translation: AAH51171.1
CCDSiCCDS35898.1 [Q8BH60-2]
RefSeqiNP_001186201.1, NM_001199272.1 [Q8BH60-1]
NP_444417.2, NM_053187.3 [Q8BH60-2]
UniGeneiMm.390258

Genome annotation databases

EnsembliENSMUST00000020008; ENSMUSP00000020008; ENSMUSG00000019861 [Q8BH60-2]
GeneIDi94221
KEGGimmu:94221
UCSCiuc007fbf.2 mouse [Q8BH60-2]
uc007fbg.2 mouse [Q8BH60-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF287893 mRNA Translation: AAG00571.1
AB052838 mRNA Translation: BAB69946.1
AK030427 mRNA Translation: BAC26958.1
AK030637 mRNA Translation: BAC27058.1
AK032613 mRNA Translation: BAC27951.1
BC051171 mRNA Translation: AAH51171.1
CCDSiCCDS35898.1 [Q8BH60-2]
RefSeqiNP_001186201.1, NM_001199272.1 [Q8BH60-1]
NP_444417.2, NM_053187.3 [Q8BH60-2]
UniGeneiMm.390258

3D structure databases

ProteinModelPortaliQ8BH60
SMRiQ8BH60
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi220478, 3 interactors
IntActiQ8BH60, 11 interactors
MINTiQ8BH60
STRINGi10090.ENSMUSP00000020008

PTM databases

iPTMnetiQ8BH60
PhosphoSitePlusiQ8BH60

Proteomic databases

PaxDbiQ8BH60
PRIDEiQ8BH60

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020008; ENSMUSP00000020008; ENSMUSG00000019861 [Q8BH60-2]
GeneIDi94221
KEGGimmu:94221
UCSCiuc007fbf.2 mouse [Q8BH60-2]
uc007fbg.2 mouse [Q8BH60-1]

Organism-specific databases

CTDi57120
MGIiMGI:2149946 Gopc

Phylogenomic databases

eggNOGiENOG410ITAF Eukaryota
ENOG410XT8J LUCA
GeneTreeiENSGT00550000074581
HOGENOMiHOG000280727
HOVERGENiHBG057507
InParanoidiQ8BH60
PhylomeDBiQ8BH60

Miscellaneous databases

ChiTaRSiGopc mouse
PROiPR:Q8BH60
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000019861 Expressed in 273 organ(s), highest expression level in ureteric bud tip
ExpressionAtlasiQ8BH60 baseline and differential
GenevisibleiQ8BH60 MM

Family and domain databases

InterProiView protein in InterPro
IPR038879 GOPC
IPR001478 PDZ
IPR036034 PDZ_sf
PANTHERiPTHR16528 PTHR16528, 1 hit
PfamiView protein in Pfam
PF00595 PDZ, 1 hit
SMARTiView protein in SMART
SM00228 PDZ, 1 hit
SUPFAMiSSF50156 SSF50156, 1 hit
PROSITEiView protein in PROSITE
PS50106 PDZ, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGOPC_MOUSE
AccessioniPrimary (citable) accession number: Q8BH60
Secondary accession number(s): Q8BSV4, Q920R1, Q9ET11
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: March 1, 2003
Last modified: September 12, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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