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Protein

Calcium-binding mitochondrial carrier protein Aralar1

Gene

Slc25a12

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mitochondrial and calcium-binding carrier that catalyzes the calcium-dependent exchange of cytoplasmic glutamate with mitochondrial aspartate across the mitochondrial inner membrane. May have a function in the urea cycle.By similarity

Miscellaneous

Binds to one calcium ion with high affinity.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi65CalciumBy similarity1
Metal bindingi67CalciumBy similarity1
Metal bindingi69CalciumBy similarity1
Metal bindingi71Calcium; via carbonyl oxygenBy similarity1
Metal bindingi76CalciumBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi65 – 761By similarityAdd BLAST12
Calcium bindingi99 – 1102PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi170 – 1813PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processTransport
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-70263 Gluconeogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-binding mitochondrial carrier protein Aralar1
Alternative name(s):
Mitochondrial aspartate glutamate carrier 1
Solute carrier family 25 member 12
Gene namesi
Name:Slc25a12
Synonyms:Aralar1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1926080 Slc25a12

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei330 – 347Helical; Name=1By similarityAdd BLAST18
Transmembranei391 – 410Helical; Name=2By similarityAdd BLAST20
Transmembranei434 – 447Helical; Name=3By similarityAdd BLAST14
Transmembranei483 – 502Helical; Name=4By similarityAdd BLAST20
Transmembranei522 – 539Helical; Name=5By similarityAdd BLAST18
Transmembranei579 – 598Helical; Name=6By similarityAdd BLAST20

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000905992 – 677Calcium-binding mitochondrial carrier protein Aralar1Add BLAST676

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ8BH59
MaxQBiQ8BH59
PaxDbiQ8BH59
PeptideAtlasiQ8BH59
PRIDEiQ8BH59

PTM databases

iPTMnetiQ8BH59
PhosphoSitePlusiQ8BH59
SwissPalmiQ8BH59

Expressioni

Gene expression databases

BgeeiENSMUSG00000027010 Expressed in 237 organ(s), highest expression level in quadriceps femoris
ExpressionAtlasiQ8BH59 baseline and differential
GenevisibleiQ8BH59 MM

Interactioni

Subunit structurei

Homodimer (via N-terminus).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi219664, 8 interactors
IntActiQ8BH59, 15 interactors
MINTiQ8BH59
STRINGi10090.ENSMUSP00000122103

Structurei

3D structure databases

ProteinModelPortaliQ8BH59
SMRiQ8BH59
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 85EF-hand 1PROSITE-ProRule annotationAdd BLAST46
Domaini86 – 121EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini122 – 156EF-hand 3PROSITE-ProRule annotationAdd BLAST35
Domaini157 – 192EF-hand 4PROSITE-ProRule annotationAdd BLAST36
Repeati324 – 416Solcar 1PROSITE-ProRule annotationAdd BLAST93
Repeati424 – 508Solcar 2PROSITE-ProRule annotationAdd BLAST85
Repeati516 – 604Solcar 3PROSITE-ProRule annotationAdd BLAST89

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 294N-terminal domainBy similarityAdd BLAST293
Regioni295 – 310Linker loop domainBy similarityAdd BLAST16
Regioni320 – 612Carrier domainBy similarityAdd BLAST293
Regioni613 – 677C-terminal domainBy similarityAdd BLAST65

Domaini

Upon calcium binding, the EF-hand-containing regulatory N-terminal domain binds to the C-terminal domain, opening a vestibule which allows the substrates to be translocated through the carrier domain. In the absence of calcium, the linker loop domain may close the vestibule, which may prevent substrates from entering the carrier domain.By similarity

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0751 Eukaryota
ENOG410XNRM LUCA
GeneTreeiENSGT00530000062944
HOGENOMiHOG000180633
HOVERGENiHBG005350
InParanoidiQ8BH59
KOiK15105
OMAiMQNQRGT
OrthoDBiEOG091G08GT
PhylomeDBiQ8BH59
TreeFamiTF313209

Family and domain databases

Gene3Di1.50.40.10, 1 hit
InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR002067 Mit_carrier
IPR018108 Mitochondrial_sb/sol_carrier
IPR023395 Mt_carrier_dom_sf
PfamiView protein in Pfam
PF00153 Mito_carr, 3 hits
PRINTSiPR00926 MITOCARRIER
SMARTiView protein in SMART
SM00054 EFh, 3 hits
SUPFAMiSSF103506 SSF103506, 1 hit
SSF47473 SSF47473, 2 hits
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 1 hit
PS50222 EF_HAND_2, 2 hits
PS50920 SOLCAR, 3 hits

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q8BH59-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAVKVHTTKR GDPHELRNIF LQYASTEVDG EHYMTPEDFV QRYLGLYNDP
60 70 80 90 100
NSNPKIVQLL AGVADQTKDG LISYQEFLAF ESVLCAPDSM FIVAFQLFDK
110 120 130 140 150
SGNGEVTFEN VKEIFGQTII HHHIPFNWDC EFIRLHFGHN RKKHLNYVEF
160 170 180 190 200
TQFLQELQLE HARQAFALKD KSKSGMISGL DFSDVMVTIR SHMLTPFVEE
210 220 230 240 250
NLVSAAGGGT SHQVSFSYFN AFNSLLNNME LVRKIYSTLA GTRKDIEVTK
260 270 280 290 300
EEFAQSAIRY GQVTPLEIDI LYQLADLYNA SGRLTLADIE RIAPLAEGAL
310 320 330 340 350
PYNLAELQRQ QSPGLGRPIW LQIAESAYRF TLGSVAGAVG ATAVYPIDLV
360 370 380 390 400
KTRMQNQRGT GSVVGELMYK NSFDCFKKVL RYEGFFGLYR GLIPQLIGVA
410 420 430 440 450
PEKAIKLTVN DFVRDKFTKR DGSIPLPAEI LAGGCAGGSQ VIFTNPLEIV
460 470 480 490 500
KIRLQVAGEI TTGPRVSALN VLQDLGLFGL YKGAKACFLR DIPFSAIYFP
510 520 530 540 550
VYAHCKLLLA DENGRVGGIN LLTAGALAGV PAASLVTPAD VIKTRLQVAA
560 570 580 590 600
RAGQTTYSGV VDCFRKILRE EGPSAFWKGT AARVFRSSPQ FGVTLVTYEL
610 620 630 640 650
LQRWFYIDFG GLKPSGSEPT PKSRIADLPP ANPDHIGGYR LATATFAGIE
660 670
NKFGLYLPKF KSPSVAVAQP KAAAAAQ
Length:677
Mass (Da):74,570
Last modified:March 1, 2003 - v1
Checksum:i488ABC7EB8227571
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
V9GXX9V9GXX9_MOUSE
Calcium-binding mitochondrial carri...
Slc25a12
87Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030595 mRNA Translation: BAC27037.1
AK083156 mRNA Translation: BAC38787.1
BX284624 Genomic DNA No translation available.
BC060505 mRNA Translation: AAH60505.1
CCDSiCCDS16113.1
RefSeqiNP_766024.1, NM_172436.3
UniGeneiMm.146696
Mm.30928

Genome annotation databases

EnsembliENSMUST00000151937; ENSMUSP00000122103; ENSMUSG00000027010
GeneIDi78830
KEGGimmu:78830
UCSCiuc008kan.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030595 mRNA Translation: BAC27037.1
AK083156 mRNA Translation: BAC38787.1
BX284624 Genomic DNA No translation available.
BC060505 mRNA Translation: AAH60505.1
CCDSiCCDS16113.1
RefSeqiNP_766024.1, NM_172436.3
UniGeneiMm.146696
Mm.30928

3D structure databases

ProteinModelPortaliQ8BH59
SMRiQ8BH59
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi219664, 8 interactors
IntActiQ8BH59, 15 interactors
MINTiQ8BH59
STRINGi10090.ENSMUSP00000122103

PTM databases

iPTMnetiQ8BH59
PhosphoSitePlusiQ8BH59
SwissPalmiQ8BH59

Proteomic databases

EPDiQ8BH59
MaxQBiQ8BH59
PaxDbiQ8BH59
PeptideAtlasiQ8BH59
PRIDEiQ8BH59

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000151937; ENSMUSP00000122103; ENSMUSG00000027010
GeneIDi78830
KEGGimmu:78830
UCSCiuc008kan.1 mouse

Organism-specific databases

CTDi8604
MGIiMGI:1926080 Slc25a12

Phylogenomic databases

eggNOGiKOG0751 Eukaryota
ENOG410XNRM LUCA
GeneTreeiENSGT00530000062944
HOGENOMiHOG000180633
HOVERGENiHBG005350
InParanoidiQ8BH59
KOiK15105
OMAiMQNQRGT
OrthoDBiEOG091G08GT
PhylomeDBiQ8BH59
TreeFamiTF313209

Enzyme and pathway databases

ReactomeiR-MMU-70263 Gluconeogenesis

Miscellaneous databases

ChiTaRSiSlc25a12 mouse
PROiPR:Q8BH59
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027010 Expressed in 237 organ(s), highest expression level in quadriceps femoris
ExpressionAtlasiQ8BH59 baseline and differential
GenevisibleiQ8BH59 MM

Family and domain databases

Gene3Di1.50.40.10, 1 hit
InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR002067 Mit_carrier
IPR018108 Mitochondrial_sb/sol_carrier
IPR023395 Mt_carrier_dom_sf
PfamiView protein in Pfam
PF00153 Mito_carr, 3 hits
PRINTSiPR00926 MITOCARRIER
SMARTiView protein in SMART
SM00054 EFh, 3 hits
SUPFAMiSSF103506 SSF103506, 1 hit
SSF47473 SSF47473, 2 hits
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 1 hit
PS50222 EF_HAND_2, 2 hits
PS50920 SOLCAR, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiCMC1_MOUSE
AccessioniPrimary (citable) accession number: Q8BH59
Secondary accession number(s): A2BFG0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: March 1, 2003
Last modified: October 10, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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