Slc25a12

Functionⁱ

Mitochondrial and calcium-binding carrier that catalyzes the calcium-dependent exchange of cytoplasmic glutamate with mitochondrial aspartate across the mitochondrial inner membrane. May have a function in the urea cycle.By similarity

Miscellaneous

Binds to one calcium ion with high affinity.By similarity

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	65	CalciumBy similarity	1
Metal bindingⁱ	67	CalciumBy similarity	1
Metal bindingⁱ	69	CalciumBy similarity	1
Metal bindingⁱ	71	Calcium; via carbonyl oxygenBy similarity	1
Metal bindingⁱ	76	CalciumBy similarity	1

Regions

Feature key	Position(s)	DescriptionActions	Length
Calcium bindingⁱ	65 – 76	1By similarityAdd BLAST	12
Calcium bindingⁱ	99 – 110	2PROSITE-ProRule annotationAdd BLAST	12
Calcium bindingⁱ	170 – 181	3PROSITE-ProRule annotationAdd BLAST	12

GO - Molecular functionⁱ

calcium ion binding Source: UniProtKB
identical protein binding Source: UniProtKB
L-aspartate transmembrane transporter activity Source: UniProtKB
L-glutamate transmembrane transporter activity Source: UniProtKB

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

aspartate transmembrane transport Source: UniProtKB
glutamate biosynthetic process Source: Ensembl
L-glutamate transmembrane transport Source: UniProtKB
malate-aspartate shuttle
Source: MGI
Inferred from mutant phenotypeⁱ
- 17213189
negative regulation of glucose catabolic process to lactate via pyruvate Source: Ensembl
positive regulation of ATP biosynthetic process Source: Ensembl
positive regulation of glucose metabolic process Source: Ensembl
positive regulation of myelination Source: Ensembl
response to calcium ion Source: UniProtKB

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Transport
Ligand	Calcium, Metal-binding

Enzyme and pathway databases

Reactomeⁱ	R-MMU-70263 Gluconeogenesis

Reactomeⁱ

R-MMU-70263 Gluconeogenesis

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Calcium-binding mitochondrial carrier protein Aralar1 Alternative name(s): Mitochondrial aspartate glutamate carrier 1 Solute carrier family 25 member 12
Gene namesⁱ	Name:Slc25a12 Synonyms:Aralar1
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 2

Organism-specific databases

MGIⁱ	MGI:1926080 Slc25a12

Topology

Feature key	Position(s)	DescriptionActions	Length
Transmembraneⁱ	330 – 347	Helical; Name=1By similarityAdd BLAST	18
Transmembraneⁱ	391 – 410	Helical; Name=2By similarityAdd BLAST	20
Transmembraneⁱ	434 – 447	Helical; Name=3By similarityAdd BLAST	14
Transmembraneⁱ	483 – 502	Helical; Name=4By similarityAdd BLAST	20
Transmembraneⁱ	522 – 539	Helical; Name=5By similarityAdd BLAST	18
Transmembraneⁱ	579 – 598	Helical; Name=6By similarityAdd BLAST	20

Keywords - Cellular componentⁱ

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		RemovedBy similarity
ChainⁱPRO_0000090599	2 – 677	Calcium-binding mitochondrial carrier protein Aralar1Add BLAST		676

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	2	N-acetylalanineBy similarity		1

Keywords - PTMⁱ

Acetylation

Proteomic databases

EPDⁱ	Q8BH59
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q8BH59
PaxDbⁱ	Q8BH59
PeptideAtlas More...PeptideAtlasⁱ	Q8BH59
PRIDEⁱ	Q8BH59

PTM databases

iPTMnetⁱ	Q8BH59
PhosphoSitePlusⁱ	Q8BH59
SwissPalmⁱ	Q8BH59

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000027010 Expressed in 237 organ(s), highest expression level in quadriceps femoris
ExpressionAtlasⁱ	Q8BH59 baseline and differential
Genevisibleⁱ	Q8BH59 MM

Interactionⁱ

Subunit structureⁱ

Homodimer (via N-terminus).By similarity

GO - Molecular functionⁱ

identical protein binding Source: UniProtKB

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	219664, 8 interactors
IntActⁱ	Q8BH59, 15 interactors
Molecular INTeraction database More...MINTⁱ	Q8BH59
STRINGⁱ	10090.ENSMUSP00000122103

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q8BH59
SMRⁱ	Q8BH59
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	40 – 85	EF-hand 1PROSITE-ProRule annotationAdd BLAST	46
Domainⁱ	86 – 121	EF-hand 2PROSITE-ProRule annotationAdd BLAST	36
Domainⁱ	122 – 156	EF-hand 3PROSITE-ProRule annotationAdd BLAST	35
Domainⁱ	157 – 192	EF-hand 4PROSITE-ProRule annotationAdd BLAST	36
Repeatⁱ	324 – 416	Solcar 1PROSITE-ProRule annotationAdd BLAST	93
Repeatⁱ	424 – 508	Solcar 2PROSITE-ProRule annotationAdd BLAST	85
Repeatⁱ	516 – 604	Solcar 3PROSITE-ProRule annotationAdd BLAST	89

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	2 – 294	N-terminal domainBy similarityAdd BLAST	293
Regionⁱ	295 – 310	Linker loop domainBy similarityAdd BLAST	16
Regionⁱ	320 – 612	Carrier domainBy similarityAdd BLAST	293
Regionⁱ	613 – 677	C-terminal domainBy similarityAdd BLAST	65

Domainⁱ

Upon calcium binding, the EF-hand-containing regulatory N-terminal domain binds to the C-terminal domain, opening a vestibule which allows the substrates to be translocated through the carrier domain. In the absence of calcium, the linker loop domain may close the vestibule, which may prevent substrates from entering the carrier domain.By similarity

Sequence similaritiesⁱ

Belongs to the mitochondrial carrier (TC 2.A.29) family. [View classification]Curated

Keywords - Domainⁱ

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	KOG0751 Eukaryota ENOG410XNRM LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00530000062944
HOGENOMⁱ	HOG000180633
HOVERGENⁱ	HBG005350
InParanoidⁱ	Q8BH59
KEGG Orthology (KO) More...KOⁱ	K15105
OMAⁱ	MQNQRGT
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G08GT
PhylomeDBⁱ	Q8BH59
TreeFamⁱ	TF313209

Family and domain databases

Gene3Dⁱ	1.50.40.10, 1 hit
InterProⁱ	View protein in InterPro IPR011992 EF-hand-dom_pair IPR018247 EF_Hand_1_Ca_BS IPR002048 EF_hand_dom IPR002067 Mit_carrier IPR018108 Mitochondrial_sb/sol_carrier IPR023395 Mt_carrier_dom_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00153 Mito_carr, 3 hits
PRINTSⁱ	PR00926 MITOCARRIER
SMARTⁱ	View protein in SMART SM00054 EFh, 3 hits
SUPFAMⁱ	SSF103506 SSF103506, 1 hit SSF47473 SSF47473, 2 hits
PROSITEⁱ	View protein in PROSITE PS00018 EF_HAND_1, 1 hit PS50222 EF_HAND_2, 2 hits PS50920 SOLCAR, 3 hits

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show all Align All

Q8BH59-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MAVKVHTTKR GDPHELRNIF LQYASTEVDG EHYMTPEDFV QRYLGLYNDP 
        60         70         80         90        100
NSNPKIVQLL AGVADQTKDG LISYQEFLAF ESVLCAPDSM FIVAFQLFDK 
       110        120        130        140        150
SGNGEVTFEN VKEIFGQTII HHHIPFNWDC EFIRLHFGHN RKKHLNYVEF 
       160        170        180        190        200
TQFLQELQLE HARQAFALKD KSKSGMISGL DFSDVMVTIR SHMLTPFVEE 
       210        220        230        240        250
NLVSAAGGGT SHQVSFSYFN AFNSLLNNME LVRKIYSTLA GTRKDIEVTK 
       260        270        280        290        300
EEFAQSAIRY GQVTPLEIDI LYQLADLYNA SGRLTLADIE RIAPLAEGAL 
       310        320        330        340        350
PYNLAELQRQ QSPGLGRPIW LQIAESAYRF TLGSVAGAVG ATAVYPIDLV 
       360        370        380        390        400
KTRMQNQRGT GSVVGELMYK NSFDCFKKVL RYEGFFGLYR GLIPQLIGVA 
       410        420        430        440        450
PEKAIKLTVN DFVRDKFTKR DGSIPLPAEI LAGGCAGGSQ VIFTNPLEIV 
       460        470        480        490        500
KIRLQVAGEI TTGPRVSALN VLQDLGLFGL YKGAKACFLR DIPFSAIYFP 
       510        520        530        540        550
VYAHCKLLLA DENGRVGGIN LLTAGALAGV PAASLVTPAD VIKTRLQVAA 
       560        570        580        590        600
RAGQTTYSGV VDCFRKILRE EGPSAFWKGT AARVFRSSPQ FGVTLVTYEL 
       610        620        630        640        650
LQRWFYIDFG GLKPSGSEPT PKSRIADLPP ANPDHIGGYR LATATFAGIE 
       660        670 
NKFGLYLPKF KSPSVAVAQP KAAAAAQ

Length:677

Mass (Da):74,570

Last modified:March 1, 2003 - v1

Checksum:ⁱ488ABC7EB8227571

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	V9GXX9	V9GXX9_MOUSE		Calcium-binding mitochondrial carri... Calcium-binding mitochondrial carrier protein Aralar1	Slc25a12	87	Annotation score:

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK030595 mRNA Translation: BAC27037.1 AK083156 mRNA Translation: BAC38787.1 BX284624 Genomic DNA No translation available. BC060505 mRNA Translation: AAH60505.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS16113.1
NCBI Reference Sequences More...RefSeqⁱ	NP_766024.1, NM_172436.3
UniGeneⁱ	Mm.146696 Mm.30928

Genome annotation databases

Ensemblⁱ	ENSMUST00000151937; ENSMUSP00000122103; ENSMUSG00000027010
GeneIDⁱ	78830
KEGGⁱ	mmu:78830
UCSC genome browser More...UCSCⁱ	uc008kan.1 mouse

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q8BH59	Calcium-binding mitochondrial carrier protein Aralar1	)	HUMAN		678	UniRef90_O75746
Calcium-binding mitochondrial carrier protein Aralar1		PONAB		678
Uncharacterized protein		SHEEP		751
Uncharacterized protein		COLAP		700
Calcium-binding mitochondrial carrier protein Aralar1		PTEAL		687
+210

Protein	Similar proteins		Species	Score	Length	Source
Q8BH59	Calcium-binding mitochondrial carrier protein Aralar1	)	HUMAN		678	UniRef50_O75746
Calcium-binding mitochondrial carrier protein Aralar1		PONAB		678
Probable calcium-binding mitochondrial carrier K02F3.2		CAEEL		716
Calcium-binding mitochondrial carrier protein Aralar1		DROME		695
Uncharacterized protein		SHEEP		751
+1400

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK030595 mRNA Translation: BAC27037.1 AK083156 mRNA Translation: BAC38787.1 BX284624 Genomic DNA No translation available. BC060505 mRNA Translation: AAH60505.1
CCDSⁱ	CCDS16113.1
RefSeqⁱ	NP_766024.1, NM_172436.3
UniGeneⁱ	Mm.146696 Mm.30928

3D structure databases

ProteinModelPortalⁱ	Q8BH59
SMRⁱ	Q8BH59
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	219664, 8 interactors
IntActⁱ	Q8BH59, 15 interactors
MINTⁱ	Q8BH59
STRINGⁱ	10090.ENSMUSP00000122103

PTM databases

iPTMnetⁱ	Q8BH59
PhosphoSitePlusⁱ	Q8BH59
SwissPalmⁱ	Q8BH59

Proteomic databases

EPDⁱ	Q8BH59
MaxQBⁱ	Q8BH59
PaxDbⁱ	Q8BH59
PeptideAtlasⁱ	Q8BH59
PRIDEⁱ	Q8BH59

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000151937; ENSMUSP00000122103; ENSMUSG00000027010
GeneIDⁱ	78830
KEGGⁱ	mmu:78830
UCSCⁱ	uc008kan.1 mouse

Organism-specific databases

CTDⁱ	8604
MGIⁱ	MGI:1926080 Slc25a12

Phylogenomic databases

eggNOGⁱ	KOG0751 Eukaryota ENOG410XNRM LUCA
GeneTreeⁱ	ENSGT00530000062944
HOGENOMⁱ	HOG000180633
HOVERGENⁱ	HBG005350
InParanoidⁱ	Q8BH59
KOⁱ	K15105
OMAⁱ	MQNQRGT
OrthoDBⁱ	EOG091G08GT
PhylomeDBⁱ	Q8BH59
TreeFamⁱ	TF313209

Enzyme and pathway databases

Reactomeⁱ	R-MMU-70263 Gluconeogenesis

Reactomeⁱ

R-MMU-70263 Gluconeogenesis

Miscellaneous databases

ChiTaRSⁱ	Slc25a12 mouse
Protein Ontology More...PROⁱ	PR:Q8BH59
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000027010 Expressed in 237 organ(s), highest expression level in quadriceps femoris
ExpressionAtlasⁱ	Q8BH59 baseline and differential
Genevisibleⁱ	Q8BH59 MM

Family and domain databases

Gene3Dⁱ	1.50.40.10, 1 hit
InterProⁱ	View protein in InterPro IPR011992 EF-hand-dom_pair IPR018247 EF_Hand_1_Ca_BS IPR002048 EF_hand_dom IPR002067 Mit_carrier IPR018108 Mitochondrial_sb/sol_carrier IPR023395 Mt_carrier_dom_sf
Pfamⁱ	View protein in Pfam PF00153 Mito_carr, 3 hits
PRINTSⁱ	PR00926 MITOCARRIER
SMARTⁱ	View protein in SMART SM00054 EFh, 3 hits
SUPFAMⁱ	SSF103506 SSF103506, 1 hit SSF47473 SSF47473, 2 hits
PROSITEⁱ	View protein in PROSITE PS00018 EF_HAND_1, 1 hit PS50222 EF_HAND_2, 2 hits PS50920 SOLCAR, 3 hits
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	CMC1_MOUSE
Accessionⁱ	Q8BH59Primary (citable) accession number: Q8BH59 Secondary accession number(s): A2BFG0
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
	Last sequence update:	March 1, 2003
	Last modified:	October 10, 2018
	This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Direct protein sequencing, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

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UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q8BH59 (CMC1_MOUSE)

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Calcium-binding mitochondrial carrier protein Aralar1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Mitochondrion

Mitochondrion

Other locations

Topology

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ