UniProtKB - Q8BH59 (CMC1_MOUSE)
Protein
Calcium-binding mitochondrial carrier protein Aralar1
Gene
Slc25a12
Organism
Mus musculus (Mouse)
Status
Functioni
Mitochondrial and calcium-binding carrier that catalyzes the calcium-dependent exchange of cytoplasmic glutamate with mitochondrial aspartate across the mitochondrial inner membrane. May have a function in the urea cycle.By similarity
Miscellaneous
Binds to one calcium ion with high affinity.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 65 | CalciumBy similarity | 1 | |
Metal bindingi | 67 | CalciumBy similarity | 1 | |
Metal bindingi | 69 | CalciumBy similarity | 1 | |
Metal bindingi | 71 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 76 | CalciumBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Calcium bindingi | 65 – 76 | 1By similarityAdd BLAST | 12 | |
Calcium bindingi | 99 – 110 | 2PROSITE-ProRule annotationAdd BLAST | 12 | |
Calcium bindingi | 170 – 181 | 3PROSITE-ProRule annotationAdd BLAST | 12 |
GO - Molecular functioni
- calcium ion binding Source: UniProtKB
- identical protein binding Source: UniProtKB
- L-aspartate transmembrane transporter activity Source: UniProtKB
- L-glutamate transmembrane transporter activity Source: UniProtKB
GO - Biological processi
- aspartate transmembrane transport Source: UniProtKB
- glutamate biosynthetic process Source: Ensembl
- L-glutamate transmembrane transport Source: UniProtKB
- malate-aspartate shuttle Source: MGI
- negative regulation of glucose catabolic process to lactate via pyruvate Source: Ensembl
- positive regulation of ATP biosynthetic process Source: Ensembl
- positive regulation of glucose metabolic process Source: Ensembl
- positive regulation of myelination Source: Ensembl
- response to calcium ion Source: UniProtKB
Keywordsi
Biological process | Transport |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
Reactomei | R-MMU-70263, Gluconeogenesis R-MMU-8963693, Aspartate and asparagine metabolism |
Names & Taxonomyi
Protein namesi | Recommended name: Calcium-binding mitochondrial carrier protein Aralar1Alternative name(s): Mitochondrial aspartate glutamate carrier 1 Solute carrier family 25 member 12 |
Gene namesi | Name:Slc25a12 Synonyms:Aralar1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1926080, Slc25a12 |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane By similarity; Multi-pass membrane protein By similarity
Mitochondrion
- mitochondrial inner membrane Source: MGI
- mitochondrion Source: MGI
Other locations
- integral component of membrane Source: UniProtKB-KW
- myelin sheath Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 330 – 347 | Helical; Name=1By similarityAdd BLAST | 18 | |
Transmembranei | 391 – 410 | Helical; Name=2By similarityAdd BLAST | 20 | |
Transmembranei | 434 – 447 | Helical; Name=3By similarityAdd BLAST | 14 | |
Transmembranei | 483 – 502 | Helical; Name=4By similarityAdd BLAST | 20 | |
Transmembranei | 522 – 539 | Helical; Name=5By similarityAdd BLAST | 18 | |
Transmembranei | 579 – 598 | Helical; Name=6By similarityAdd BLAST | 20 |
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000090599 | 2 – 677 | Calcium-binding mitochondrial carrier protein Aralar1Add BLAST | 676 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 |
Keywords - PTMi
AcetylationProteomic databases
EPDi | Q8BH59 |
jPOSTi | Q8BH59 |
MaxQBi | Q8BH59 |
PaxDbi | Q8BH59 |
PeptideAtlasi | Q8BH59 |
PRIDEi | Q8BH59 |
PTM databases
iPTMneti | Q8BH59 |
PhosphoSitePlusi | Q8BH59 |
SwissPalmi | Q8BH59 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000027010, Expressed in quadriceps femoris and 256 other tissues |
ExpressionAtlasi | Q8BH59, baseline and differential |
Genevisiblei | Q8BH59, MM |
Interactioni
Subunit structurei
Homodimer (via N-terminus).
By similarityGO - Molecular functioni
- identical protein binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 219664, 16 interactors |
IntActi | Q8BH59, 18 interactors |
MINTi | Q8BH59 |
STRINGi | 10090.ENSMUSP00000122103 |
Miscellaneous databases
RNActi | Q8BH59, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 40 – 85 | EF-hand 1PROSITE-ProRule annotationAdd BLAST | 46 | |
Domaini | 86 – 121 | EF-hand 2PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 122 – 156 | EF-hand 3PROSITE-ProRule annotationAdd BLAST | 35 | |
Domaini | 157 – 192 | EF-hand 4PROSITE-ProRule annotationAdd BLAST | 36 | |
Repeati | 324 – 416 | Solcar 1PROSITE-ProRule annotationAdd BLAST | 93 | |
Repeati | 424 – 508 | Solcar 2PROSITE-ProRule annotationAdd BLAST | 85 | |
Repeati | 516 – 604 | Solcar 3PROSITE-ProRule annotationAdd BLAST | 89 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2 – 294 | N-terminal domainBy similarityAdd BLAST | 293 | |
Regioni | 295 – 310 | Linker loop domainBy similarityAdd BLAST | 16 | |
Regioni | 320 – 612 | Carrier domainBy similarityAdd BLAST | 293 | |
Regioni | 613 – 677 | C-terminal domainBy similarityAdd BLAST | 65 |
Domaini
Upon calcium binding, the EF-hand-containing regulatory N-terminal domain binds to the C-terminal domain, opening a vestibule which allows the substrates to be translocated through the carrier domain. In the absence of calcium, the linker loop domain may close the vestibule, which may prevent substrates from entering the carrier domain.By similarity
Sequence similaritiesi
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0751, Eukaryota |
GeneTreei | ENSGT00940000155963 |
HOGENOMi | CLU_014931_3_0_1 |
InParanoidi | Q8BH59 |
OMAi | KKDFFGE |
OrthoDBi | 1007928at2759 |
PhylomeDBi | Q8BH59 |
TreeFami | TF313209 |
Family and domain databases
Gene3Di | 1.50.40.10, 1 hit |
InterProi | View protein in InterPro IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR002067, Mit_carrier IPR018108, Mitochondrial_sb/sol_carrier IPR023395, Mt_carrier_dom_sf |
Pfami | View protein in Pfam PF00153, Mito_carr, 3 hits |
PRINTSi | PR00926, MITOCARRIER |
SMARTi | View protein in SMART SM00054, EFh, 3 hits |
SUPFAMi | SSF103506, SSF103506, 1 hit SSF47473, SSF47473, 2 hits |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 1 hit PS50222, EF_HAND_2, 2 hits PS50920, SOLCAR, 3 hits |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q8BH59-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAVKVHTTKR GDPHELRNIF LQYASTEVDG EHYMTPEDFV QRYLGLYNDP
60 70 80 90 100
NSNPKIVQLL AGVADQTKDG LISYQEFLAF ESVLCAPDSM FIVAFQLFDK
110 120 130 140 150
SGNGEVTFEN VKEIFGQTII HHHIPFNWDC EFIRLHFGHN RKKHLNYVEF
160 170 180 190 200
TQFLQELQLE HARQAFALKD KSKSGMISGL DFSDVMVTIR SHMLTPFVEE
210 220 230 240 250
NLVSAAGGGT SHQVSFSYFN AFNSLLNNME LVRKIYSTLA GTRKDIEVTK
260 270 280 290 300
EEFAQSAIRY GQVTPLEIDI LYQLADLYNA SGRLTLADIE RIAPLAEGAL
310 320 330 340 350
PYNLAELQRQ QSPGLGRPIW LQIAESAYRF TLGSVAGAVG ATAVYPIDLV
360 370 380 390 400
KTRMQNQRGT GSVVGELMYK NSFDCFKKVL RYEGFFGLYR GLIPQLIGVA
410 420 430 440 450
PEKAIKLTVN DFVRDKFTKR DGSIPLPAEI LAGGCAGGSQ VIFTNPLEIV
460 470 480 490 500
KIRLQVAGEI TTGPRVSALN VLQDLGLFGL YKGAKACFLR DIPFSAIYFP
510 520 530 540 550
VYAHCKLLLA DENGRVGGIN LLTAGALAGV PAASLVTPAD VIKTRLQVAA
560 570 580 590 600
RAGQTTYSGV VDCFRKILRE EGPSAFWKGT AARVFRSSPQ FGVTLVTYEL
610 620 630 640 650
LQRWFYIDFG GLKPSGSEPT PKSRIADLPP ANPDHIGGYR LATATFAGIE
660 670
NKFGLYLPKF KSPSVAVAQP KAAAAAQ
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketV9GXX9 | V9GXX9_MOUSE | Calcium-binding mitochondrial carri... | Slc25a12 | 87 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK030595 mRNA Translation: BAC27037.1 AK083156 mRNA Translation: BAC38787.1 BX284624 Genomic DNA No translation available. BC060505 mRNA Translation: AAH60505.1 |
CCDSi | CCDS16113.1 |
RefSeqi | NP_766024.1, NM_172436.3 |
Genome annotation databases
Ensembli | ENSMUST00000151937; ENSMUSP00000122103; ENSMUSG00000027010 |
GeneIDi | 78830 |
KEGGi | mmu:78830 |
UCSCi | uc008kan.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK030595 mRNA Translation: BAC27037.1 AK083156 mRNA Translation: BAC38787.1 BX284624 Genomic DNA No translation available. BC060505 mRNA Translation: AAH60505.1 |
CCDSi | CCDS16113.1 |
RefSeqi | NP_766024.1, NM_172436.3 |
3D structure databases
SMRi | Q8BH59 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 219664, 16 interactors |
IntActi | Q8BH59, 18 interactors |
MINTi | Q8BH59 |
STRINGi | 10090.ENSMUSP00000122103 |
PTM databases
iPTMneti | Q8BH59 |
PhosphoSitePlusi | Q8BH59 |
SwissPalmi | Q8BH59 |
Proteomic databases
EPDi | Q8BH59 |
jPOSTi | Q8BH59 |
MaxQBi | Q8BH59 |
PaxDbi | Q8BH59 |
PeptideAtlasi | Q8BH59 |
PRIDEi | Q8BH59 |
Protocols and materials databases
Antibodypediai | 33855, 209 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000151937; ENSMUSP00000122103; ENSMUSG00000027010 |
GeneIDi | 78830 |
KEGGi | mmu:78830 |
UCSCi | uc008kan.1, mouse |
Organism-specific databases
CTDi | 8604 |
MGIi | MGI:1926080, Slc25a12 |
Phylogenomic databases
eggNOGi | KOG0751, Eukaryota |
GeneTreei | ENSGT00940000155963 |
HOGENOMi | CLU_014931_3_0_1 |
InParanoidi | Q8BH59 |
OMAi | KKDFFGE |
OrthoDBi | 1007928at2759 |
PhylomeDBi | Q8BH59 |
TreeFami | TF313209 |
Enzyme and pathway databases
Reactomei | R-MMU-70263, Gluconeogenesis R-MMU-8963693, Aspartate and asparagine metabolism |
Miscellaneous databases
BioGRID-ORCSi | 78830, 1 hit in 18 CRISPR screens |
ChiTaRSi | Slc25a12, mouse |
PROi | PR:Q8BH59 |
RNActi | Q8BH59, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000027010, Expressed in quadriceps femoris and 256 other tissues |
ExpressionAtlasi | Q8BH59, baseline and differential |
Genevisiblei | Q8BH59, MM |
Family and domain databases
Gene3Di | 1.50.40.10, 1 hit |
InterProi | View protein in InterPro IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR002067, Mit_carrier IPR018108, Mitochondrial_sb/sol_carrier IPR023395, Mt_carrier_dom_sf |
Pfami | View protein in Pfam PF00153, Mito_carr, 3 hits |
PRINTSi | PR00926, MITOCARRIER |
SMARTi | View protein in SMART SM00054, EFh, 3 hits |
SUPFAMi | SSF103506, SSF103506, 1 hit SSF47473, SSF47473, 2 hits |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 1 hit PS50222, EF_HAND_2, 2 hits PS50920, SOLCAR, 3 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CMC1_MOUSE | |
Accessioni | Q8BH59Primary (citable) accession number: Q8BH59 Secondary accession number(s): A2BFG0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 24, 2004 |
Last sequence update: | March 1, 2003 | |
Last modified: | February 10, 2021 | |
This is version 161 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families