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Protein

Complement component C8 beta chain

Gene

C8b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processComplement alternate pathway, Complement pathway, Cytolysis, Immunity, Innate immunity

Enzyme and pathway databases

ReactomeiR-MMU-166665 Terminal pathway of complement
R-MMU-977606 Regulation of Complement cascade

Names & Taxonomyi

Protein namesi
Recommended name:
Complement component C8 beta chain
Alternative name(s):
Complement component 8 subunit beta
Gene namesi
Name:C8b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:88236 C8b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane attack complex, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Sequence analysisAdd BLAST31
PropeptideiPRO_000002359332 – 53By similarityAdd BLAST22
ChainiPRO_000002359454 – 589Complement component C8 beta chainAdd BLAST536

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi43N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi64 ↔ 99By similarity
Glycosylationi69C-linked (Man) tryptophanBy similarity1
Glycosylationi72C-linked (Man) tryptophanBy similarity1
Disulfide bondi75 ↔ 78By similarity
Disulfide bondi109 ↔ 115By similarity
Disulfide bondi121 ↔ 132By similarity
Disulfide bondi126 ↔ 145By similarity
Disulfide bondi139 ↔ 154By similarity
Glycosylationi252N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi377 ↔ 402By similarity
Modified residuei417PhosphothreonineBy similarity1
Disulfide bondi502 ↔ 549By similarity
Disulfide bondi504 ↔ 520By similarity
Disulfide bondi507 ↔ 522By similarity
Disulfide bondi524 ↔ 533By similarity
Glycosylationi550C-linked (Man) tryptophanBy similarity1
Glycosylationi553C-linked (Man) tryptophanBy similarity1
Disulfide bondi556 ↔ 589By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ8BH35
PeptideAtlasiQ8BH35
PRIDEiQ8BH35

PTM databases

iPTMnetiQ8BH35
PhosphoSitePlusiQ8BH35

Expressioni

Gene expression databases

BgeeiENSMUSG00000029656
CleanExiMM_C8B
GenevisibleiQ8BH35 MM

Interactioni

Subunit structurei

Heterotrimer of 3 chains: alpha, beta and gamma. The alpha and gamma chains are disulfide bonded. Component of the membrane attack complex (MAC). MAC assembly is initiated by proteolytic cleavage of C5 into C5a and C5b. C5b sequentially binds C6, C7, C8 and multiple copies of the pore-forming subunit C9 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000031663

Structurei

3D structure databases

ProteinModelPortaliQ8BH35
SMRiQ8BH35
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini63 – 116TSP type-1 1PROSITE-ProRule annotationAdd BLAST54
Domaini120 – 155LDL-receptor class APROSITE-ProRule annotationAdd BLAST36
Domaini157 – 503MACPFPROSITE-ProRule annotationAdd BLAST347
Domaini404 – 534EGF-likeAdd BLAST131
Domaini544 – 587TSP type-1 2PROSITE-ProRule annotationAdd BLAST44

Sequence similaritiesi

Belongs to the complement C6/C7/C8/C9 family.Curated

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IE7H Eukaryota
ENOG410Y2J1 LUCA
GeneTreeiENSGT00550000074478
HOGENOMiHOG000231146
HOVERGENiHBG106489
InParanoidiQ8BH35
KOiK03998
OMAiLMLHYEF
OrthoDBiEOG091G07LK
PhylomeDBiQ8BH35
TreeFamiTF330498

Family and domain databases

CDDicd00112 LDLa, 1 hit
Gene3Di2.20.100.10, 2 hits
InterProiView protein in InterPro
IPR037566 Complement_C8_beta
IPR036055 LDL_receptor-like_sf
IPR023415 LDLR_class-A_CS
IPR002172 LDrepeatLR_classA_rpt
IPR001862 MAC_perforin
IPR020864 MACPF
IPR020863 MACPF_CS
IPR000884 TSP1_rpt
IPR036383 TSP1_rpt_sf
PANTHERiPTHR19325:SF401 PTHR19325:SF401, 1 hit
PfamiView protein in Pfam
PF00057 Ldl_recept_a, 1 hit
PF01823 MACPF, 1 hit
PF00090 TSP_1, 2 hits
PRINTSiPR00764 COMPLEMENTC9
SMARTiView protein in SMART
SM00192 LDLa, 1 hit
SM00457 MACPF, 1 hit
SM00209 TSP1, 2 hits
SUPFAMiSSF57424 SSF57424, 1 hit
SSF82895 SSF82895, 2 hits
PROSITEiView protein in PROSITE
PS00022 EGF_1, 1 hit
PS01186 EGF_2, 1 hit
PS01209 LDLRA_1, 1 hit
PS50068 LDLRA_2, 1 hit
PS00279 MACPF_1, 1 hit
PS51412 MACPF_2, 1 hit
PS50092 TSP1, 2 hits

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BH35-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKIGAQVWRA LAKSCLLCAT LGCLHFPGSR GGKPDFFETK AVNGSLVKSR
60 70 80 90 100
PVRSVAEAPA PIDCELSTWS SWTACDPCQK KRYRHTYLLR PSQFYGELCD
110 120 130 140 150
LSDKEVEDCV TNQPCRSQVR CEGFVCAQTG RCVNRRLLCN GDNDCGDQSD
160 170 180 190 200
EANCRRIYKN CQREMEQYWA IDRLASGINL FTNTFEGPVL DHRYYAGGCS
210 220 230 240 250
PHYILDTNFR KPYNVESYTP QTKCEYEFTL TEYESYSDFE RLVIEKKTHM
260 270 280 290 300
FNFTSGFKVD GVMDLGIKVE SNEGKNYVTR TKRFAHTQSK FLHARSVLEV
310 320 330 340 350
AHYKLKSRSL MLHYEFLQRV KSLPLEYSYG EYRDLLRDFG THFITEAVLG
360 370 380 390 400
GIYEYTLIMN KDAMEQGDYT LSHVTACAGG SFGIGGMVYK VYVKVGVSAK
410 420 430 440 450
KCSDIMKEIN ERNKRSTMVE DLVVLVRGGT SEDITALAYK ELPTPELMEA
460 470 480 490 500
WGDAVKYNPA IIKIKAEPLY ELVTATDFAY SSTVKQNLKK ALEEFQSEVS
510 520 530 540 550
SCRCAPCRGN GVPVLKGSRC ECICPGGFQG TACEVTYRKD IPIDGKWSCW
560 570 580
SDWSACSGGH KTRHRQCNNP APHKGGSPCS GPASETLNC
Length:589
Mass (Da):66,229
Last modified:March 1, 2003 - v1
Checksum:i540B299AFBC408A0
GO
Isoform 2 (identifier: Q8BH35-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     222-287: Missing.

Show »
Length:523
Mass (Da):58,450
Checksum:iF86F45617E4709DB
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti14S → W in strain: Mae/Stm. 1
Natural varianti19A → T in strain: MSM/Ms. 1
Natural varianti60A → E in strain: Mae/Stm. 1
Natural varianti118Q → R in strain: MSM/Ms. 1
Natural varianti160N → K in strain: Mae/Stm. 1
Natural varianti449E → K in strain: Mae/Stm and MSM/Ms. 1
Natural varianti497S → R in strain: Mae/Stm and MSM/Ms. 1
Natural varianti503R → H in strain: Mae/Stm and MSM/Ms. 1
Natural varianti564H → Q in strain: Mae/Stm. 1
Natural varianti569N → S in strain: MSM/Ms. 1
Natural varianti577S → N in strain: Mae/Stm and MSM/Ms. 1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_016666222 – 287Missing in isoform 2. 1 PublicationAdd BLAST66

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB077304 mRNA Translation: BAC41369.1
AB077305 mRNA Translation: BAC41370.1
AB077306 mRNA Translation: BAC41371.1
AK050313 mRNA Translation: BAC34183.1
BC022129 mRNA Translation: AAH22129.1
BC096382 mRNA Translation: AAH96382.1
CCDSiCCDS18414.1 [Q8BH35-1]
CCDS84762.1 [Q8BH35-2]
RefSeqiNP_001303600.1, NM_001316671.1 [Q8BH35-2]
NP_598643.1, NM_133882.2 [Q8BH35-1]
UniGeneiMm.87072

Genome annotation databases

EnsembliENSMUST00000031663; ENSMUSP00000031663; ENSMUSG00000029656 [Q8BH35-1]
ENSMUST00000065072; ENSMUSP00000066940; ENSMUSG00000029656 [Q8BH35-2]
GeneIDi110382
KEGGimmu:110382
UCSCiuc008txy.1 mouse [Q8BH35-1]
uc012dhv.1 mouse [Q8BH35-2]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCO8B_MOUSE
AccessioniPrimary (citable) accession number: Q8BH35
Secondary accession number(s): Q4VAH1
, Q8CHJ5, Q8CHJ6, Q8VC14
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 1, 2003
Last modified: June 20, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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