UniProtKB - Q8BH35 (CO8B_MOUSE)
Complement component C8 beta chain
C8b
Functioni
GO - Molecular functioni
- protein-containing complex binding Source: MGI
GO - Biological processi
- complement activation, alternative pathway Source: UniProtKB-KW
- complement activation, classical pathway Source: UniProtKB-KW
- cytolysis Source: UniProtKB-KW
Keywordsi
Biological process | Complement alternate pathway, Complement pathway, Cytolysis, Immunity, Innate immunity |
Enzyme and pathway databases
Reactomei | R-MMU-166665, Terminal pathway of complement R-MMU-977606, Regulation of Complement cascade |
Names & Taxonomyi
Protein namesi | Recommended name: Complement component C8 beta chainAlternative name(s): Complement component 8 subunit beta |
Gene namesi | Name:C8b |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:88236, C8b |
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular region Source: MGI
- extracellular space Source: MGI
Plasma Membrane
- membrane attack complex Source: MGI
Keywords - Cellular componenti
Membrane attack complex, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 31 | Sequence analysisAdd BLAST | 31 | |
PropeptideiPRO_0000023593 | 32 – 53 | By similarityAdd BLAST | 22 | |
ChainiPRO_0000023594 | 54 – 589 | Complement component C8 beta chainAdd BLAST | 536 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 43 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 64 ↔ 99 | By similarity | ||
Glycosylationi | 69 | C-linked (Man) tryptophanBy similarity | 1 | |
Glycosylationi | 72 | C-linked (Man) tryptophanBy similarity | 1 | |
Disulfide bondi | 75 ↔ 78 | By similarity | ||
Disulfide bondi | 109 ↔ 115 | By similarity | ||
Disulfide bondi | 121 ↔ 132 | By similarity | ||
Disulfide bondi | 126 ↔ 145 | By similarity | ||
Disulfide bondi | 139 ↔ 154 | By similarity | ||
Glycosylationi | 252 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 377 ↔ 402 | By similarity | ||
Modified residuei | 417 | PhosphothreonineBy similarity | 1 | |
Disulfide bondi | 502 ↔ 549 | By similarity | ||
Disulfide bondi | 504 ↔ 520 | By similarity | ||
Disulfide bondi | 507 ↔ 522 | By similarity | ||
Disulfide bondi | 524 ↔ 533 | By similarity | ||
Glycosylationi | 550 | C-linked (Man) tryptophanBy similarity | 1 | |
Glycosylationi | 553 | C-linked (Man) tryptophanBy similarity | 1 | |
Disulfide bondi | 556 ↔ 589 | By similarity |
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
CPTACi | non-CPTAC-3535 non-CPTAC-5590 |
jPOSTi | Q8BH35 |
PaxDbi | Q8BH35 |
PeptideAtlasi | Q8BH35 |
PRIDEi | Q8BH35 |
PTM databases
GlyGeni | Q8BH35, 6 sites |
iPTMneti | Q8BH35 |
PhosphoSitePlusi | Q8BH35 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000029656, Expressed in liver and 69 other tissues |
Genevisiblei | Q8BH35, MM |
Interactioni
Subunit structurei
Heterotrimer of 3 chains: alpha, beta and gamma. The alpha and gamma chains are disulfide bonded.
Component of the membrane attack complex (MAC). MAC assembly is initiated by proteolytic cleavage of C5 into C5a and C5b. C5b sequentially binds C6, C7, C8 and multiple copies of the pore-forming subunit C9 (By similarity).
By similarityProtein-protein interaction databases
BioGRIDi | 225551, 3 interactors |
STRINGi | 10090.ENSMUSP00000031663 |
Miscellaneous databases
RNActi | Q8BH35, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 63 – 116 | TSP type-1 1PROSITE-ProRule annotationAdd BLAST | 54 | |
Domaini | 120 – 155 | LDL-receptor class APROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 157 – 503 | MACPFPROSITE-ProRule annotationAdd BLAST | 347 | |
Domaini | 404 – 534 | EGF-likeAdd BLAST | 131 | |
Domaini | 544 – 587 | TSP type-1 2PROSITE-ProRule annotationAdd BLAST | 44 |
Sequence similaritiesi
Keywords - Domaini
EGF-like domain, Repeat, SignalPhylogenomic databases
eggNOGi | KOG3535, Eukaryota |
GeneTreei | ENSGT00940000160247 |
HOGENOMi | CLU_032453_1_0_1 |
InParanoidi | Q8BH35 |
OMAi | GSCLPHY |
OrthoDBi | 787014at2759 |
PhylomeDBi | Q8BH35 |
TreeFami | TF330498 |
Family and domain databases
CDDi | cd00112, LDLa, 1 hit |
Gene3Di | 2.20.100.10, 2 hits 4.10.400.10, 1 hit |
InterProi | View protein in InterPro IPR037566, Complement_C8_beta IPR036055, LDL_receptor-like_sf IPR023415, LDLR_class-A_CS IPR002172, LDrepeatLR_classA_rpt IPR001862, MAC_perforin IPR020864, MACPF IPR020863, MACPF_CS IPR000884, TSP1_rpt IPR036383, TSP1_rpt_sf |
PANTHERi | PTHR45742:SF5, PTHR45742:SF5, 1 hit |
Pfami | View protein in Pfam PF00057, Ldl_recept_a, 1 hit PF01823, MACPF, 1 hit PF00090, TSP_1, 2 hits |
PRINTSi | PR00764, COMPLEMENTC9 |
SMARTi | View protein in SMART SM00192, LDLa, 1 hit SM00457, MACPF, 1 hit SM00209, TSP1, 2 hits |
SUPFAMi | SSF57424, SSF57424, 1 hit SSF82895, SSF82895, 2 hits |
PROSITEi | View protein in PROSITE PS00022, EGF_1, 1 hit PS01186, EGF_2, 1 hit PS01209, LDLRA_1, 1 hit PS50068, LDLRA_2, 1 hit PS00279, MACPF_1, 1 hit PS51412, MACPF_2, 1 hit PS50092, TSP1, 2 hits |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MKIGAQVWRA LAKSCLLCAT LGCLHFPGSR GGKPDFFETK AVNGSLVKSR
60 70 80 90 100
PVRSVAEAPA PIDCELSTWS SWTACDPCQK KRYRHTYLLR PSQFYGELCD
110 120 130 140 150
LSDKEVEDCV TNQPCRSQVR CEGFVCAQTG RCVNRRLLCN GDNDCGDQSD
160 170 180 190 200
EANCRRIYKN CQREMEQYWA IDRLASGINL FTNTFEGPVL DHRYYAGGCS
210 220 230 240 250
PHYILDTNFR KPYNVESYTP QTKCEYEFTL TEYESYSDFE RLVIEKKTHM
260 270 280 290 300
FNFTSGFKVD GVMDLGIKVE SNEGKNYVTR TKRFAHTQSK FLHARSVLEV
310 320 330 340 350
AHYKLKSRSL MLHYEFLQRV KSLPLEYSYG EYRDLLRDFG THFITEAVLG
360 370 380 390 400
GIYEYTLIMN KDAMEQGDYT LSHVTACAGG SFGIGGMVYK VYVKVGVSAK
410 420 430 440 450
KCSDIMKEIN ERNKRSTMVE DLVVLVRGGT SEDITALAYK ELPTPELMEA
460 470 480 490 500
WGDAVKYNPA IIKIKAEPLY ELVTATDFAY SSTVKQNLKK ALEEFQSEVS
510 520 530 540 550
SCRCAPCRGN GVPVLKGSRC ECICPGGFQG TACEVTYRKD IPIDGKWSCW
560 570 580
SDWSACSGGH KTRHRQCNNP APHKGGSPCS GPASETLNC
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 14 | S → W in strain: Mae/Stm. | 1 | |
Natural varianti | 19 | A → T in strain: MSM/Ms. | 1 | |
Natural varianti | 60 | A → E in strain: Mae/Stm. | 1 | |
Natural varianti | 118 | Q → R in strain: MSM/Ms. | 1 | |
Natural varianti | 160 | N → K in strain: Mae/Stm. | 1 | |
Natural varianti | 449 | E → K in strain: Mae/Stm and MSM/Ms. | 1 | |
Natural varianti | 497 | S → R in strain: Mae/Stm and MSM/Ms. | 1 | |
Natural varianti | 503 | R → H in strain: Mae/Stm and MSM/Ms. | 1 | |
Natural varianti | 564 | H → Q in strain: Mae/Stm. | 1 | |
Natural varianti | 569 | N → S in strain: MSM/Ms. | 1 | |
Natural varianti | 577 | S → N in strain: Mae/Stm and MSM/Ms. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_016666 | 222 – 287 | Missing in isoform 2. 1 PublicationAdd BLAST | 66 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB077304 mRNA Translation: BAC41369.1 AB077305 mRNA Translation: BAC41370.1 AB077306 mRNA Translation: BAC41371.1 AK050313 mRNA Translation: BAC34183.1 BC022129 mRNA Translation: AAH22129.1 BC096382 mRNA Translation: AAH96382.1 |
CCDSi | CCDS18414.1 [Q8BH35-1] CCDS84762.1 [Q8BH35-2] |
RefSeqi | NP_001303600.1, NM_001316671.1 [Q8BH35-2] NP_598643.1, NM_133882.2 [Q8BH35-1] |
Genome annotation databases
Ensembli | ENSMUST00000031663; ENSMUSP00000031663; ENSMUSG00000029656 [Q8BH35-1] ENSMUST00000065072; ENSMUSP00000066940; ENSMUSG00000029656 [Q8BH35-2] |
GeneIDi | 110382 |
KEGGi | mmu:110382 |
UCSCi | uc008txy.1, mouse [Q8BH35-1] uc012dhv.1, mouse [Q8BH35-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB077304 mRNA Translation: BAC41369.1 AB077305 mRNA Translation: BAC41370.1 AB077306 mRNA Translation: BAC41371.1 AK050313 mRNA Translation: BAC34183.1 BC022129 mRNA Translation: AAH22129.1 BC096382 mRNA Translation: AAH96382.1 |
CCDSi | CCDS18414.1 [Q8BH35-1] CCDS84762.1 [Q8BH35-2] |
RefSeqi | NP_001303600.1, NM_001316671.1 [Q8BH35-2] NP_598643.1, NM_133882.2 [Q8BH35-1] |
3D structure databases
SMRi | Q8BH35 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 225551, 3 interactors |
STRINGi | 10090.ENSMUSP00000031663 |
PTM databases
GlyGeni | Q8BH35, 6 sites |
iPTMneti | Q8BH35 |
PhosphoSitePlusi | Q8BH35 |
Proteomic databases
CPTACi | non-CPTAC-3535 non-CPTAC-5590 |
jPOSTi | Q8BH35 |
PaxDbi | Q8BH35 |
PeptideAtlasi | Q8BH35 |
PRIDEi | Q8BH35 |
Protocols and materials databases
Antibodypediai | 19370, 201 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000031663; ENSMUSP00000031663; ENSMUSG00000029656 [Q8BH35-1] ENSMUST00000065072; ENSMUSP00000066940; ENSMUSG00000029656 [Q8BH35-2] |
GeneIDi | 110382 |
KEGGi | mmu:110382 |
UCSCi | uc008txy.1, mouse [Q8BH35-1] uc012dhv.1, mouse [Q8BH35-2] |
Organism-specific databases
CTDi | 732 |
MGIi | MGI:88236, C8b |
Phylogenomic databases
eggNOGi | KOG3535, Eukaryota |
GeneTreei | ENSGT00940000160247 |
HOGENOMi | CLU_032453_1_0_1 |
InParanoidi | Q8BH35 |
OMAi | GSCLPHY |
OrthoDBi | 787014at2759 |
PhylomeDBi | Q8BH35 |
TreeFami | TF330498 |
Enzyme and pathway databases
Reactomei | R-MMU-166665, Terminal pathway of complement R-MMU-977606, Regulation of Complement cascade |
Miscellaneous databases
BioGRID-ORCSi | 110382, 0 hits in 17 CRISPR screens |
PROi | PR:Q8BH35 |
RNActi | Q8BH35, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000029656, Expressed in liver and 69 other tissues |
Genevisiblei | Q8BH35, MM |
Family and domain databases
CDDi | cd00112, LDLa, 1 hit |
Gene3Di | 2.20.100.10, 2 hits 4.10.400.10, 1 hit |
InterProi | View protein in InterPro IPR037566, Complement_C8_beta IPR036055, LDL_receptor-like_sf IPR023415, LDLR_class-A_CS IPR002172, LDrepeatLR_classA_rpt IPR001862, MAC_perforin IPR020864, MACPF IPR020863, MACPF_CS IPR000884, TSP1_rpt IPR036383, TSP1_rpt_sf |
PANTHERi | PTHR45742:SF5, PTHR45742:SF5, 1 hit |
Pfami | View protein in Pfam PF00057, Ldl_recept_a, 1 hit PF01823, MACPF, 1 hit PF00090, TSP_1, 2 hits |
PRINTSi | PR00764, COMPLEMENTC9 |
SMARTi | View protein in SMART SM00192, LDLa, 1 hit SM00457, MACPF, 1 hit SM00209, TSP1, 2 hits |
SUPFAMi | SSF57424, SSF57424, 1 hit SSF82895, SSF82895, 2 hits |
PROSITEi | View protein in PROSITE PS00022, EGF_1, 1 hit PS01186, EGF_2, 1 hit PS01209, LDLRA_1, 1 hit PS50068, LDLRA_2, 1 hit PS00279, MACPF_1, 1 hit PS51412, MACPF_2, 1 hit PS50092, TSP1, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CO8B_MOUSE | |
Accessioni | Q8BH35Primary (citable) accession number: Q8BH35 Secondary accession number(s): Q4VAH1 Q8VC14 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 15, 2005 |
Last sequence update: | March 1, 2003 | |
Last modified: | February 10, 2021 | |
This is version 144 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families