UniProtKB - Q8BH31 (MFSD8_MOUSE)
Protein
Major facilitator superfamily domain-containing protein 8
Gene
Mfsd8
Organism
Mus musculus (Mouse)
Status
Functioni
May be a carrier that transport small solutes by using chemiosmotic ion gradients.Curated
GO - Molecular functioni
- transmembrane transporter activity Source: InterPro
GO - Biological processi
- autophagosome maturation Source: MGI
- lysosome organization Source: MGI
- neuron development Source: MGI
- regulation of autophagy Source: MGI
- regulation of lysosomal protein catabolic process Source: MGI
- TORC1 signaling Source: MGI
Keywordsi
Biological process | Transport |
Names & Taxonomyi
Protein namesi | Recommended name: Major facilitator superfamily domain-containing protein 8 |
Gene namesi | Name:Mfsd8 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1919425, Mfsd8 |
Subcellular locationi
Lysosome
- Lysosome membrane By similarity; Multi-pass membrane protein By similarity
Note: Sorting to lysosomes involves tyrosine- and/or dileucine-based motifs.By similarity
Lysosome
- lysosomal membrane Source: UniProtKB-SubCell
- lysosome Source: MGI
Nucleus
- nucleoplasm Source: MGI
Other locations
- integral component of membrane Source: UniProtKB-KW
- intracellular membrane-bounded organelle Source: MGI
- membrane Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 41 | CytoplasmicSequence analysisAdd BLAST | 41 | |
Transmembranei | 42 – 62 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 63 – 75 | ExtracellularSequence analysisAdd BLAST | 13 | |
Transmembranei | 76 – 96 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 97 – 106 | CytoplasmicSequence analysis | 10 | |
Transmembranei | 107 – 127 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 128 – 140 | ExtracellularSequence analysisAdd BLAST | 13 | |
Transmembranei | 141 – 161 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 162 – 174 | CytoplasmicSequence analysisAdd BLAST | 13 | |
Transmembranei | 175 – 195 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 196 – 212 | ExtracellularSequence analysisAdd BLAST | 17 | |
Transmembranei | 213 – 233 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 234 – 267 | CytoplasmicSequence analysisAdd BLAST | 34 | |
Transmembranei | 268 – 288 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 289 – 310 | ExtracellularSequence analysisAdd BLAST | 22 | |
Transmembranei | 311 – 331 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 332 – 338 | CytoplasmicSequence analysis | 7 | |
Transmembranei | 339 – 359 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 360 – 416 | ExtracellularSequence analysisAdd BLAST | 57 | |
Transmembranei | 417 – 439 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 440 – 452 | CytoplasmicSequence analysisAdd BLAST | 13 | |
Transmembranei | 453 – 473 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 474 – 483 | ExtracellularSequence analysis | 10 | |
Transmembranei | 484 – 504 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 505 – 519 | CytoplasmicSequence analysisAdd BLAST | 15 |
Keywords - Cellular componenti
Lysosome, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000311233 | 1 – 519 | Major facilitator superfamily domain-containing protein 8Add BLAST | 519 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 372 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 377 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
EPDi | Q8BH31 |
MaxQBi | Q8BH31 |
PaxDbi | Q8BH31 |
PRIDEi | Q8BH31 |
PTM databases
GlyGeni | Q8BH31, 2 sites |
iPTMneti | Q8BH31 |
PhosphoSitePlusi | Q8BH31 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000025759, Expressed in heart left ventricle and 269 other tissues |
Genevisiblei | Q8BH31, MM |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000026859 |
Miscellaneous databases
RNActi | Q8BH31, protein |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 13 – 14 | Dileucine internalization motifBy similarity | 2 |
Sequence similaritiesi
Belongs to the major facilitator superfamily.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2325, Eukaryota |
GeneTreei | ENSGT00530000063854 |
HOGENOMi | CLU_027024_2_0_1 |
InParanoidi | Q8BH31 |
OMAi | DAGKEFM |
OrthoDBi | 685352at2759 |
PhylomeDBi | Q8BH31 |
TreeFami | TF316590 |
Family and domain databases
InterProi | View protein in InterPro IPR011701, MFS IPR020846, MFS_dom IPR036259, MFS_trans_sf |
Pfami | View protein in Pfam PF07690, MFS_1, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
Q8BH31-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MANLGSEAER EPLLGPGSPG SREWSEIETQ EHYKSRWKSV RILYLTMFLS
60 70 80 90 100
SVGFSIVIMS IWPYLQKIDQ TADASFLGWV IASYSLGQMV ASPLFGLWSN
110 120 130 140 150
YRPRKEPLIV SISISVAANC LYAYVHVPAA HNKYYMLIAR GLVGFGAGNV
160 170 180 190 200
AVVRSYIAGA TSLQERTNAM ANTSTCQALG FILGPVFQTC FALIGEKGVT
210 220 230 240 250
WDIIKLQVNM YTAPVLLAAF LGILNIILIL FILREHRVDD LGRQCKSVNF
260 270 280 290 300
QEENTDEPQI PEGSIDQVAV VATNIVFFVV LFIFAVYETI LTPLTLDMYA
310 320 330 340 350
WTQEQAVLYD GILLVAFGVE AVLVFMGVKL LSKKIGERAI LLGGFVVVWV
360 370 380 390 400
GFFILLPWGN QFPKIQWEDL HNSSTPNTTF GEIIIGLWNS SREDHSEQPT
410 420 430 440 450
GCPIEQTWCL YTPVIHLAQF LTAAVLIGTG YPACSVMSYT LYSKVLGPKP
460 470 480 490 500
QGIYMGWLTT SGSAARILGP VFISHVYTYL GPRWAFSLVC GIVVLTILLI
510
GAVYKRLVAF SVRYMRIQE
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0N4SUV8 | A0A0N4SUV8_MOUSE | Major facilitator superfamily domai... | Mfsd8 | 187 | Annotation score: | ||
F6R8A9 | F6R8A9_MOUSE | Major facilitator superfamily domai... | Mfsd8 | 93 | Annotation score: | ||
A0A0N4SWC1 | A0A0N4SWC1_MOUSE | Major facilitator superfamily domai... | Mfsd8 | 36 | Annotation score: | ||
A0A0N4SVE9 | A0A0N4SVE9_MOUSE | Major facilitator superfamily domai... | Mfsd8 | 44 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 25 | S → G in AAH10483 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 39 | S → F in BAB28676 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 50 | S → N in BAB28676 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 116 | V → G in BAB28676 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 129 | A → T in BAB28676 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 313 | L → F in AAH10483 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 391 | S → P in AAH10483 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK013144 mRNA Translation: BAB28676.1 AK032080 mRNA Translation: BAC27687.1 AK077957 mRNA Translation: BAC37083.1 BC010483 mRNA Translation: AAH10483.1 BC113183 mRNA Translation: AAI13184.1 BC113790 mRNA Translation: AAI13791.1 |
CCDSi | CCDS17329.1 |
RefSeqi | NP_082416.2, NM_028140.4 |
Genome annotation databases
Ensembli | ENSMUST00000026859; ENSMUSP00000026859; ENSMUSG00000025759 ENSMUST00000229555; ENSMUSP00000155107; ENSMUSG00000116169 |
GeneIDi | 72175 |
KEGGi | mmu:72175 |
UCSCi | uc008pbp.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK013144 mRNA Translation: BAB28676.1 AK032080 mRNA Translation: BAC27687.1 AK077957 mRNA Translation: BAC37083.1 BC010483 mRNA Translation: AAH10483.1 BC113183 mRNA Translation: AAI13184.1 BC113790 mRNA Translation: AAI13791.1 |
CCDSi | CCDS17329.1 |
RefSeqi | NP_082416.2, NM_028140.4 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000026859 |
PTM databases
GlyGeni | Q8BH31, 2 sites |
iPTMneti | Q8BH31 |
PhosphoSitePlusi | Q8BH31 |
Proteomic databases
EPDi | Q8BH31 |
MaxQBi | Q8BH31 |
PaxDbi | Q8BH31 |
PRIDEi | Q8BH31 |
Protocols and materials databases
Antibodypediai | 45354, 58 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000026859; ENSMUSP00000026859; ENSMUSG00000025759 ENSMUST00000229555; ENSMUSP00000155107; ENSMUSG00000116169 |
GeneIDi | 72175 |
KEGGi | mmu:72175 |
UCSCi | uc008pbp.1, mouse |
Organism-specific databases
CTDi | 256471 |
MGIi | MGI:1919425, Mfsd8 |
Phylogenomic databases
eggNOGi | KOG2325, Eukaryota |
GeneTreei | ENSGT00530000063854 |
HOGENOMi | CLU_027024_2_0_1 |
InParanoidi | Q8BH31 |
OMAi | DAGKEFM |
OrthoDBi | 685352at2759 |
PhylomeDBi | Q8BH31 |
TreeFami | TF316590 |
Miscellaneous databases
BioGRID-ORCSi | 72175, 2 hits in 17 CRISPR screens |
ChiTaRSi | Mfsd8, mouse |
PROi | PR:Q8BH31 |
RNActi | Q8BH31, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000025759, Expressed in heart left ventricle and 269 other tissues |
Genevisiblei | Q8BH31, MM |
Family and domain databases
InterProi | View protein in InterPro IPR011701, MFS IPR020846, MFS_dom IPR036259, MFS_trans_sf |
Pfami | View protein in Pfam PF07690, MFS_1, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MFSD8_MOUSE | |
Accessioni | Q8BH31Primary (citable) accession number: Q8BH31 Secondary accession number(s): Q91VS1, Q9CZ06 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 13, 2007 |
Last sequence update: | March 1, 2003 | |
Last modified: | December 2, 2020 | |
This is version 121 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families