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Protein

Phosphoenolpyruvate carboxykinase [GTP], mitochondrial

Gene

Pck2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.By similarity

Miscellaneous

In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one.

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.

Cofactori

Mn2+By similarityNote: Binds 1 Mn2+ ion per subunit.By similarity

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei104SubstrateBy similarity1
Metal bindingi262ManganeseBy similarity1
Metal bindingi282Manganese; via tele nitrogenBy similarity1
Binding sitei304SubstrateBy similarity1
Active sitei306By similarity1
Metal bindingi329ManganeseBy similarity1
Binding sitei423GTPBy similarity1
Binding sitei454GTPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi305 – 310GTPBy similarity6
Nucleotide bindingi548 – 551GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylase, Lyase
Biological processGluconeogenesis
LigandGTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-70263 Gluconeogenesis
UniPathwayiUPA00138

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP], mitochondrial (EC:4.1.1.32)
Short name:
PEPCK-M
Gene namesi
Name:Pck2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1860456 Pck2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 32MitochondrionBy similarityAdd BLAST32
ChainiPRO_000002356933 – 640Phosphoenolpyruvate carboxykinase [GTP], mitochondrialAdd BLAST608

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei88N6-acetyllysineBy similarity1
Modified residuei115PhosphoserineBy similarity1
Modified residuei196PhosphothreonineBy similarity1
Modified residuei304PhosphoserineCombined sources1
Modified residuei457N6-succinyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8BH04
MaxQBiQ8BH04
PaxDbiQ8BH04
PeptideAtlasiQ8BH04
PRIDEiQ8BH04

PTM databases

iPTMnetiQ8BH04
PhosphoSitePlusiQ8BH04
SwissPalmiQ8BH04

Expressioni

Gene expression databases

ExpressionAtlasiQ8BH04 baseline and differential

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

IntActiQ8BH04, 1 interactor
MINTiQ8BH04
STRINGi10090.ENSMUSP00000038555

Structurei

3D structure databases

ProteinModelPortaliQ8BH04
SMRiQ8BH04
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni253 – 255Substrate bindingBy similarity3
Regioni421 – 423Substrate bindingBy similarity3

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG3749 Eukaryota
COG1274 LUCA
GeneTreeiENSGT00390000001912
HOGENOMiHOG000191700
HOVERGENiHBG053651
InParanoidiQ8BH04
KOiK01596
PhylomeDBiQ8BH04
TreeFamiTF314402

Family and domain databases

CDDicd00819 PEPCK_GTP, 1 hit
Gene3Di3.40.449.10, 2 hits
3.90.228.20, 2 hits
HAMAPiMF_00452 PEPCK_GTP, 1 hit
InterProiView protein in InterPro
IPR018091 PEP_carboxykin_GTP_CS
IPR013035 PEP_carboxykinase_C
IPR008209 PEP_carboxykinase_GTP
IPR035077 PEP_carboxykinase_GTP_C
IPR035078 PEP_carboxykinase_GTP_N
IPR008210 PEP_carboxykinase_N
PANTHERiPTHR11561 PTHR11561, 1 hit
PfamiView protein in Pfam
PF00821 PEPCK_C, 1 hit
PF17297 PEPCK_N, 1 hit
PIRSFiPIRSF001348 PEP_carboxykinase_GTP, 1 hit
SUPFAMiSSF68923 SSF68923, 1 hit
PROSITEiView protein in PROSITE
PS00505 PEPCK_GTP, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8BH04-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAMYLPGLR LSRHGLRPWC WSPCRSIQTL HVLSGDMSQL PAGVRDFVAR
60 70 80 90 100
SAHLCQPEGI HICDGTEAEN TAILALLEEQ GLIRKLPKYK NCWLARTDPK
110 120 130 140 150
DVARVESKTV IVTPSQRDTV PLLAGGARGQ LGNWMSPDEF QRAVDERFPG
160 170 180 190 200
CMQGRIMYVL PFSMGPVGSP LSRIGVQLTD SAYVVASMRI MTRLGTPVLQ
210 220 230 240 250
ALGDGDFIKC LHSVGQPLTG HGDPVGQWPC NPEKTLIGHV PDQREIVSFG
260 270 280 290 300
SGYGGNSLLG KKCFALRIAS RLARDEGWLA EHMLILGITN PAGKKRYVAA
310 320 330 340 350
AFPSACGKTN LAMMRPALPG WKVECVGDDI AWMRFDSEGQ LRAINPENGF
360 370 380 390 400
FGVAPGTSAA TNPNAMATIQ SNTLFTNVAE TSDGGVYWEG IDQPLPPGVT
410 420 430 440 450
ITSWLGKPWK PGDKEPCAHP NSRFCVPARQ CPIMDPAWEA PEGVPIDAII
460 470 480 490 500
FGGRRPKGVP LVYEAFNWRH GVFVGSAMRS ESTAAAEHKG KTIMHDPFAM
510 520 530 540 550
RPFFGYNFGR YLEHWLSMEG QKGARLPRIF HVNWFRRDEA GRFLWPGFGE
560 570 580 590 600
NARVLDWICR RLEGEDSAQE TPIGLVPKEG ALDLSGLSAV DTSQLFSIPK
610 620 630 640
DFWEQEVRDI RGYLTEQVNQ DLPKEVLAEL EALEGRVQKM
Length:640
Mass (Da):70,528
Last modified:March 1, 2003 - v1
Checksum:i5CE06D4DF5BBF44E
GO

Sequence cautioni

The sequence BAC26991 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti31H → R in AAH10318 (PubMed:15489334).Curated1
Sequence conflicti542R → C in AAH10318 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030501 mRNA Translation: BAC26991.1 Different initiation.
AK031612 mRNA Translation: BAC27477.1
AK034927 mRNA Translation: BAC28883.1
BC010318 mRNA Translation: AAH10318.1
CCDSiCCDS27114.1
RefSeqiNP_083270.1, NM_028994.2
UniGeneiMm.29856
Mm.491140

Genome annotation databases

EnsembliENSMUST00000226519; ENSMUSP00000153733; ENSMUSG00000040618
GeneIDi74551
KEGGimmu:74551
UCSCiuc007tyy.1 mouse

Similar proteinsi

Entry informationi

Entry nameiPCKGM_MOUSE
AccessioniPrimary (citable) accession number: Q8BH04
Secondary accession number(s): Q8BMM9, Q91Z10
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: March 1, 2003
Last modified: June 20, 2018
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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