UniProtKB - Q8BGW0 (THMS1_MOUSE)
Protein
Protein THEMIS
Gene
Themis
Organism
Mus musculus (Mouse)
Status
Functioni
Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk.5 Publications
GO - Biological processi
- adaptive immune response Source: UniProtKB-KW
- negative T cell selection Source: UniProtKB
- positive T cell selection Source: UniProtKB
- T cell receptor signaling pathway Source: UniProtKB
Keywordsi
Molecular function | Developmental protein |
Biological process | Adaptive immunity, Immunity |
Names & Taxonomyi
Protein namesi | Recommended name: Protein THEMISAlternative name(s): Grb2-associating protein Short name: Gasp Protein thylex Thymocyte-expressed molecule involved in selection |
Gene namesi | Name:Themis |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2443552, Themis |
Subcellular locationi
Nucleus
- COP9 signalosome Source: UniProtKB
- nucleus Source: UniProtKB
Other locations
- cell-cell junction Source: MGI
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
Mice are viable, are born at the expected Mendelian frequency and present no gross abnormalities. They however display a blockade in late T-cell development with defects in thymocyte selection. The number of transitional CD4+CD8(int) thymocytes as well as CD4+ or CD8+ single-positive thymocytes is lower. Thymocytes show defective positive selection, resulting in fewer mature thymocytes. Negative selection is also impaired. A greater percentage of T-cells have CD4+CD25+Foxp3+ regulatory and CD62L(lo)CD44(hi) memory phenotypes compared to wild-type T-cells.5 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 512 | T → P: Decreased levels of protein expression in thymocytes. Reduced numbers of CD4 and CD8 single-positive thymocytes as well as reduced numbers of peripheral CD4 and CD8 T-cells. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000252379 | 1 – 636 | Protein THEMISAdd BLAST | 636 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 586 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Phosphorylated on Tyr residues quickly after TCR stimulation.2 Publications
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q8BGW0 |
jPOSTi | Q8BGW0 |
PaxDbi | Q8BGW0 |
PRIDEi | Q8BGW0 |
PTM databases
iPTMneti | Q8BGW0 |
PhosphoSitePlusi | Q8BGW0 |
Expressioni
Tissue specificityi
Expressed in the thymus and to a lesser extent in the spleen but not detectable in non-lymphoid tissues. Highly expressed in thymocytes between the pre-T-cell antigen receptor (pre-TCR) and positive-selection checkpoints and expressed at low level in mature T-cells (at protein level).4 Publications
Inductioni
Down-regulated by stimulation through the alpha-beta TCR.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000049109, Expressed in thymus and 61 other tissues |
Genevisiblei | Q8BGW0, MM |
Interactioni
Subunit structurei
Interacts with PLCG1, ITK, GRB2, and LAT.
5 PublicationsBinary interactionsi
Protein-protein interaction databases
DIPi | DIP-48976N |
IntActi | Q8BGW0, 8 interactors |
MINTi | Q8BGW0 |
STRINGi | 10090.ENSMUSP00000060129 |
Miscellaneous databases
RNActi | Q8BGW0, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 260 | CABIT 1Add BLAST | 260 | |
Regioni | 261 – 520 | CABIT 2Add BLAST | 260 |
Sequence similaritiesi
Belongs to the themis family.Curated
Phylogenomic databases
eggNOGi | ENOG502QSJR, Eukaryota |
GeneTreei | ENSGT00530000063770 |
HOGENOMi | CLU_022319_1_0_1 |
InParanoidi | Q8BGW0 |
OMAi | KVPVGCQ |
OrthoDBi | 337909at2759 |
PhylomeDBi | Q8BGW0 |
TreeFami | TF333479 |
Family and domain databases
InterProi | View protein in InterPro IPR025946, CABIT_dom IPR039671, THEMIS |
PANTHERi | PTHR15215, PTHR15215, 1 hit |
Pfami | View protein in Pfam PF12736, CABIT, 2 hits |
s (5+)i Sequence
Sequence statusi: Complete.
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 5 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8BGW0-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MALSLEEFVY SLDLRTLPRV LEIQSGIYFE GSVYEMFGNE CCLSTGEVIK
60 70 80 90 100
ITGLKIKKMM AEICEGAIGG CESQKPFELP MNFPGLFKVM ADKTPYLSIE
110 120 130 140 150
EITRTVNIGP SRLGHPCFYH LKDIKLENLI IKQGEPIRFN SVEEINGETL
160 170 180 190 200
VNCGVVRNHQ SHSFTLPLSQ EGEFYECEDE HIYTLKEIVE WKIPKNRTRT
210 220 230 240 250
VKLTDFSNKW DSTNPFPEDF YGTLILKPVY EIQGVLKFQK DIVRILPSLD
260 270 280 290 300
VEVKDITDSY DANWFLQLLS TDDLFEMTSK EFPVVAEVVE ISQGNHLPQS
310 320 330 340 350
ILQREKTIVI HKKYQASRIL ASEIRSNFPK RHFLIPISYK GKFKRRPREF
360 370 380 390 400
PTAYDLQIAK SRKETLHVVA TKAFHTLHKE LSPVSVGDQF LVHHSETTEV
410 420 430 440 450
VFEGTRKVNV LTCEKVLNKT REDAQLPLYM EGGFVEVIHD KKQYQISELC
460 470 480 490 500
TQFCWPFNVK VAVRDLSIKD DILAATPGLQ LEEDITDSYL LISDFANPEE
510 520 530 540 550
CWEIPMSRLN MTVRLVNGSS LPADAGLLQV RSFVEEITEE QYYMMRRYES
560 570 580 590 600
SLSHPPPRPP KHPSAEEMKL TLLSLAEERT INLPKSLKSH HVDRPKKLPS
610 620 630
DESGQDSRAP VGFQNDVADV ERQKSKHGPL QPQAPL
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE0CYT7 | E0CYT7_MOUSE | Protein THEMIS | Themis | 203 | Annotation score: | ||
E0CY68 | E0CY68_MOUSE | Protein THEMIS | Themis | 94 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 152 | N → S in BAE41228 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_020919 | 1 – 89 | Missing in isoform 4. 1 PublicationAdd BLAST | 89 | |
Alternative sequenceiVSP_020920 | 238 – 262 | FQKDI…DSYDA → CKYPLRMLSCVSLHCGIWAR EAFRT in isoform 5. 1 PublicationAdd BLAST | 25 | |
Alternative sequenceiVSP_020921 | 263 – 636 | Missing in isoform 5. 1 PublicationAdd BLAST | 374 | |
Alternative sequenceiVSP_020922 | 589 – 636 | SHHVD…PQAPL → YLSNNKSHDWKPEDDEA in isoform 2. 1 PublicationAdd BLAST | 48 | |
Alternative sequenceiVSP_020923 | 589 – 636 | SHHVD…PQAPL → DRTPCCC in isoform 3. 1 PublicationAdd BLAST | 48 | |
Alternative sequenceiVSP_020924 | 589 – 636 | SHHVD…PQAPL → NSSKLKNIKNNKT in isoform 4. 1 PublicationAdd BLAST | 48 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB509340 mRNA Translation: BAH79575.1 AK040594 mRNA Translation: BAC30638.1 AK041386 mRNA Translation: BAC30927.1 AK041562 mRNA Translation: BAC30986.1 AK041622 mRNA Translation: BAC31008.1 AK088136 mRNA Translation: BAC40167.1 AK089077 mRNA Translation: BAC40738.1 AK138701 mRNA Translation: BAE23752.1 AK169558 mRNA Translation: BAE41228.1 CH466540 Genomic DNA Translation: EDL04825.1 BC125486 mRNA Translation: AAI25487.1 BC125488 mRNA Translation: AAI25489.1 |
CCDSi | CCDS23758.1 [Q8BGW0-1] CCDS87977.1 [Q8BGW0-3] |
RefSeqi | NP_001292592.1, NM_001305663.1 [Q8BGW0-4] NP_848781.1, NM_178666.6 [Q8BGW0-1] |
Genome annotation databases
Ensembli | ENSMUST00000056097; ENSMUSP00000060129; ENSMUSG00000049109 [Q8BGW0-1] ENSMUST00000060409; ENSMUSP00000055315; ENSMUSG00000049109 [Q8BGW0-2] ENSMUST00000105516; ENSMUSP00000101155; ENSMUSG00000049109 [Q8BGW0-3] |
GeneIDi | 210757 |
KEGGi | mmu:210757 |
UCSCi | uc007eso.2, mouse [Q8BGW0-5] uc007esp.2, mouse [Q8BGW0-1] uc007esq.2, mouse [Q8BGW0-4] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB509340 mRNA Translation: BAH79575.1 AK040594 mRNA Translation: BAC30638.1 AK041386 mRNA Translation: BAC30927.1 AK041562 mRNA Translation: BAC30986.1 AK041622 mRNA Translation: BAC31008.1 AK088136 mRNA Translation: BAC40167.1 AK089077 mRNA Translation: BAC40738.1 AK138701 mRNA Translation: BAE23752.1 AK169558 mRNA Translation: BAE41228.1 CH466540 Genomic DNA Translation: EDL04825.1 BC125486 mRNA Translation: AAI25487.1 BC125488 mRNA Translation: AAI25489.1 |
CCDSi | CCDS23758.1 [Q8BGW0-1] CCDS87977.1 [Q8BGW0-3] |
RefSeqi | NP_001292592.1, NM_001305663.1 [Q8BGW0-4] NP_848781.1, NM_178666.6 [Q8BGW0-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
DIPi | DIP-48976N |
IntActi | Q8BGW0, 8 interactors |
MINTi | Q8BGW0 |
STRINGi | 10090.ENSMUSP00000060129 |
PTM databases
iPTMneti | Q8BGW0 |
PhosphoSitePlusi | Q8BGW0 |
Proteomic databases
EPDi | Q8BGW0 |
jPOSTi | Q8BGW0 |
PaxDbi | Q8BGW0 |
PRIDEi | Q8BGW0 |
Protocols and materials databases
Antibodypediai | 49883, 231 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000056097; ENSMUSP00000060129; ENSMUSG00000049109 [Q8BGW0-1] ENSMUST00000060409; ENSMUSP00000055315; ENSMUSG00000049109 [Q8BGW0-2] ENSMUST00000105516; ENSMUSP00000101155; ENSMUSG00000049109 [Q8BGW0-3] |
GeneIDi | 210757 |
KEGGi | mmu:210757 |
UCSCi | uc007eso.2, mouse [Q8BGW0-5] uc007esp.2, mouse [Q8BGW0-1] uc007esq.2, mouse [Q8BGW0-4] |
Organism-specific databases
CTDi | 387357 |
MGIi | MGI:2443552, Themis |
Phylogenomic databases
eggNOGi | ENOG502QSJR, Eukaryota |
GeneTreei | ENSGT00530000063770 |
HOGENOMi | CLU_022319_1_0_1 |
InParanoidi | Q8BGW0 |
OMAi | KVPVGCQ |
OrthoDBi | 337909at2759 |
PhylomeDBi | Q8BGW0 |
TreeFami | TF333479 |
Miscellaneous databases
BioGRID-ORCSi | 210757, 0 hits in 12 CRISPR screens |
ChiTaRSi | Themis, mouse |
PROi | PR:Q8BGW0 |
RNActi | Q8BGW0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000049109, Expressed in thymus and 61 other tissues |
Genevisiblei | Q8BGW0, MM |
Family and domain databases
InterProi | View protein in InterPro IPR025946, CABIT_dom IPR039671, THEMIS |
PANTHERi | PTHR15215, PTHR15215, 1 hit |
Pfami | View protein in Pfam PF12736, CABIT, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | THMS1_MOUSE | |
Accessioni | Q8BGW0Primary (citable) accession number: Q8BGW0 Secondary accession number(s): Q059Y0 Q8C9S6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 17, 2006 |
Last sequence update: | March 1, 2003 | |
Last modified: | December 2, 2020 | |
This is version 117 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families