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Protein

Mitochondrial dynamics protein MID51

Gene

Mief1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mitochondrial outer membrane protein which regulates mitochondrial fission. Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface independently of the mitochondrial fission FIS1 and MFF proteins. Regulates DNM1L GTPase activity and DNM1L oligomerization. Binds ADP and can also bind GDP, although with lower affinity. Does not bind CDP, UDP, ATP, AMP or GTP. Inhibits DNM1L GTPase activity in the absence of bound ADP. Requires ADP to stimulate DNM1L GTPase activity and the assembly of DNM1L into long, oligomeric tubules with a spiral pattern, as opposed to the ring-like DNM1L oligomers observed in the absence of bound ADP. Does not require ADP for its function in recruiting DNM1L.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei187ADP1 Publication1
Binding sitei189ADP1 Publication1
Binding sitei201ADP1 Publication1
Binding sitei340ADP1 Publication1
Binding sitei342ADP1 Publication1
Binding sitei368ADP1 Publication1

GO - Molecular functioni

  • ADP binding Source: MGI
  • GDP binding Source: MGI
  • identical protein binding Source: IntAct

GO - Biological processi

Keywordsi

LigandNucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial dynamics protein MID51
Alternative name(s):
Mitochondrial dynamics protein of 51 kDa homolog
Mitochondrial elongation factor 1
Smith-Magenis syndrome chromosomal region candidate gene 7 protein-like
Gene namesi
Name:Mief1
Synonyms:Mid51, Smcr7l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:2146020 Mief1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 23Mitochondrial intermembraneSequence analysisAdd BLAST23
Transmembranei24 – 46HelicalSequence analysisAdd BLAST23
Topological domaini47 – 463CytoplasmicSequence analysisAdd BLAST417

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi189S → A: Abolishes ADP binding. 1 Publication1
Mutagenesisi201H → A: Abolishes ADP binding. 1 Publication1
Mutagenesisi234 – 237RREN → AAEA: Abolishes interaction with DNM1L. 4
Mutagenesisi239 – 243EYFPR → AAFPA: Impairs interaction with DNM1L. 5
Mutagenesisi253 – 255VGG → EEE: Impairs interaction with DNM1L. 3
Mutagenesisi368K → A: Mildly reduces affinity for ADP. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003104491 – 463Mitochondrial dynamics protein MID51Add BLAST463

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei55PhosphoserineBy similarity1
Modified residuei59PhosphoserineBy similarity1
Modified residuei79PhosphoserineCombined sources1
Modified residuei94PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8BGV8
PaxDbiQ8BGV8
PRIDEiQ8BGV8

PTM databases

iPTMnetiQ8BGV8
PhosphoSitePlusiQ8BGV8

Expressioni

Gene expression databases

BgeeiENSMUSG00000022412 Expressed in 243 organ(s), highest expression level in cardiac ventricle
ExpressionAtlasiQ8BGV8 baseline and differential
GenevisibleiQ8BGV8 MM

Interactioni

Subunit structurei

Homodimer. Interacts with DNM1L.1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

CORUMiQ8BGV8
DIPiDIP-60660N
IntActiQ8BGV8, 1 interactor
STRINGi10090.ENSMUSP00000023048

Structurei

Secondary structure

1463
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ8BGV8
SMRiQ8BGV8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni49 – 195DimerizationBy similarityAdd BLAST147
Regioni160 – 169Important for interaction with DNM1LBy similarity10
Regioni234 – 243Important for interaction with DNM1L1 Publication10

Sequence similaritiesi

Belongs to the MID49/MID51 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IK9T Eukaryota
ENOG41109RH LUCA
GeneTreeiENSGT00390000013127
HOGENOMiHOG000038002
HOVERGENiHBG054078
InParanoidiQ8BGV8
OMAiEDTIMNI
OrthoDBiEOG091G079I
PhylomeDBiQ8BGV8
TreeFamiTF331032

Family and domain databases

InterProiView protein in InterPro
IPR024810 Mab-21_dom
PfamiView protein in Pfam
PF03281 Mab-21, 1 hit
SMARTiView protein in SMART
SM01265 Mab-21, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q8BGV8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAGAGERKGK KDDNGIGTAI DFVLSNARLV LGVGGAAMLG IATLAVKRMY
60 70 80 90 100
DRAISAPTSP TRLSHSGKRS WEEPNWMGSP RLLNKDMKAG LSRSLQTLPT
110 120 130 140 150
DSSAFDTDTF CPPRPKPLAR RGQVDLKKSR LRMSLQEKLL SYYRNRAAIP
160 170 180 190 200
AGEQARAKQA AVDICAELRS FLRAKLPDMP LRDMYLSGSL YDDLQVVTAD
210 220 230 240 250
HIQLIVPLVL EQNLWSCIPG EDTIMNVPGF FLVRRENPEY FPRGSSYWDR
260 270 280 290 300
CVVGGYLSPK TVADTFEKVV AGSINWPAIG SLLDYVIRPA PPPEALTLEV
310 320 330 340 350
QYEKDKHLVI DFLPSVTLGD TVLVARPHRL AQYDNLWRLS LRPAETARLR
360 370 380 390 400
ALDQADSGCR SLCLKILKAI CKSTPALGHL TASQLTNVIL HLAQEEADWS
410 420 430 440 450
PDMLADRFLQ ALRGLISYLE AGVLPSALNP KVNLFAELTP QEIDELGYTL
460
YCSLSEPEVL LQT
Length:463
Mass (Da):51,184
Last modified:March 1, 2003 - v1
Checksum:i69C8869807974024
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A2U3TZF4A0A2U3TZF4_MOUSE
Mitochondrial dynamics protein MID5...
Mief1
137Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti444D → N in BAC37896 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK038466 mRNA Translation: BAC30010.1
AK044773 mRNA Translation: BAC32082.1
AK050546 mRNA Translation: BAC34317.1
AK080375 mRNA Translation: BAC37896.1
CCDSiCCDS27661.1
RefSeqiNP_848834.2, NM_178719.5
XP_006521009.1, XM_006520946.3
XP_006521010.1, XM_006520947.3
UniGeneiMm.307163

Genome annotation databases

EnsembliENSMUST00000023048; ENSMUSP00000023048; ENSMUSG00000022412
ENSMUST00000166030; ENSMUSP00000129209; ENSMUSG00000022412
ENSMUST00000229138; ENSMUSP00000154875; ENSMUSG00000022412
GeneIDi239555
KEGGimmu:239555
UCSCiuc007wvh.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK038466 mRNA Translation: BAC30010.1
AK044773 mRNA Translation: BAC32082.1
AK050546 mRNA Translation: BAC34317.1
AK080375 mRNA Translation: BAC37896.1
CCDSiCCDS27661.1
RefSeqiNP_848834.2, NM_178719.5
XP_006521009.1, XM_006520946.3
XP_006521010.1, XM_006520947.3
UniGeneiMm.307163

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4OAFX-ray2.20A/B/C/D134-463[»]
4OAGX-ray2.00A/B134-463[»]
4OAHX-ray2.00A/B/C/D134-463[»]
4OAIX-ray2.00Z134-463[»]
ProteinModelPortaliQ8BGV8
SMRiQ8BGV8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

CORUMiQ8BGV8
DIPiDIP-60660N
IntActiQ8BGV8, 1 interactor
STRINGi10090.ENSMUSP00000023048

PTM databases

iPTMnetiQ8BGV8
PhosphoSitePlusiQ8BGV8

Proteomic databases

MaxQBiQ8BGV8
PaxDbiQ8BGV8
PRIDEiQ8BGV8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023048; ENSMUSP00000023048; ENSMUSG00000022412
ENSMUST00000166030; ENSMUSP00000129209; ENSMUSG00000022412
ENSMUST00000229138; ENSMUSP00000154875; ENSMUSG00000022412
GeneIDi239555
KEGGimmu:239555
UCSCiuc007wvh.2 mouse

Organism-specific databases

CTDi54471
MGIiMGI:2146020 Mief1

Phylogenomic databases

eggNOGiENOG410IK9T Eukaryota
ENOG41109RH LUCA
GeneTreeiENSGT00390000013127
HOGENOMiHOG000038002
HOVERGENiHBG054078
InParanoidiQ8BGV8
OMAiEDTIMNI
OrthoDBiEOG091G079I
PhylomeDBiQ8BGV8
TreeFamiTF331032

Miscellaneous databases

PROiPR:Q8BGV8
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022412 Expressed in 243 organ(s), highest expression level in cardiac ventricle
ExpressionAtlasiQ8BGV8 baseline and differential
GenevisibleiQ8BGV8 MM

Family and domain databases

InterProiView protein in InterPro
IPR024810 Mab-21_dom
PfamiView protein in Pfam
PF03281 Mab-21, 1 hit
SMARTiView protein in SMART
SM01265 Mab-21, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMID51_MOUSE
AccessioniPrimary (citable) accession number: Q8BGV8
Secondary accession number(s): Q8C4Y9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: March 1, 2003
Last modified: September 12, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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