UniProtKB - Q8BGV7 (BACD1_MOUSE)
Protein
BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 1
Gene
Kctd13
Organism
Mus musculus (Mouse)
Status
Functioni
Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for synaptic transmission (PubMed:29088697). The BCR(KCTD13) E3 ubiquitin ligase complex mediates the ubiquitination of RHOA, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and promoting synaptic transmission (PubMed:29088697).1 Publication
Caution
Down-regulation of Kctd13 was initially reported to cause macrocephaly due to increased proliferation (PubMed:22596160). However, it was later shown that deletion of Kctd13 does not cause any change in brain size, embryonic cell proliferation, neurogenesis, or cortical layering/migration (PubMed:29088697). Experimental conditions used may explain discrepancies. A possible explanation being that shRNAs used in the first study, may have affected off-targets.2 Publications
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.By similarityView all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
GO - Molecular functioni
- GTP-Rho binding Source: UniProtKB
- identical protein binding Source: MGI
- protein domain specific binding Source: MGI
- ubiquitin-protein transferase activity Source: Ensembl
GO - Biological processi
- cell migration Source: UniProtKB
- DNA replication Source: MGI
- negative regulation of Rho protein signal transduction Source: UniProtKB
- positive regulation of DNA replication Source: MGI
- positive regulation of synaptic transmission Source: UniProtKB
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: UniProtKB
- protein homooligomerization Source: InterPro
- protein ubiquitination Source: UniProtKB
- stress fiber assembly Source: UniProtKB
Keywordsi
Biological process | Ubl conjugation pathway |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 1Alternative name(s): BTB/POZ domain-containing protein KCTD13 Polymerase delta-interacting protein 1 |
Gene namesi | Name:Kctd13 Synonyms:Poldip1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1923739, Kctd13 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Nucleus
- nuclear body Source: MGI
- nucleoplasm Source: MGI
Other locations
- Cul3-RING ubiquitin ligase complex Source: UniProtKB
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
Reduced synaptic transmission in area CA1 of the hippocampus caused by increased levels of RHOA. Brain size or neural progenitor cell proliferation are not affected.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000191298 | 1 – 329 | BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 1Add BLAST | 329 |
Proteomic databases
EPDi | Q8BGV7 |
MaxQBi | Q8BGV7 |
PaxDbi | Q8BGV7 |
PRIDEi | Q8BGV7 |
PTM databases
PhosphoSitePlusi | Q8BGV7 |
Expressioni
Inductioni
By TNF-alpha.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000030685, Expressed in cardiac ventricle and 213 other tissues |
Genevisiblei | Q8BGV7, MM |
Interactioni
Subunit structurei
Homotetramer; forms a two-fold symmetric tetramer in solution.
Interacts with CUL3; interaction is direct and forms a 5:5 heterodecamer.
Component of the BCR(KCTD13) E3 ubiquitin ligase complex, at least composed of CUL3, KCTD13/BACURD1 and RBX1.
Interacts with RHOA; with a preference for RhoA-GDP.
Interacts with POLD2 and PCNA.
Interacts with SPRTN.
By similarityGO - Molecular functioni
- GTP-Rho binding Source: UniProtKB
- identical protein binding Source: MGI
- protein domain specific binding Source: MGI
Protein-protein interaction databases
IntActi | Q8BGV7, 1 interactor |
STRINGi | 10090.ENSMUSP00000032924 |
Miscellaneous databases
RNActi | Q8BGV7, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 41 – 109 | BTBPROSITE-ProRule annotationAdd BLAST | 69 |
Sequence similaritiesi
Belongs to the BACURD family.Curated
Phylogenomic databases
eggNOGi | KOG2716, Eukaryota |
GeneTreei | ENSGT00950000183143 |
HOGENOMi | CLU_060008_0_0_1 |
InParanoidi | Q8BGV7 |
OMAi | HYTTLQT |
OrthoDBi | 1306250at2759 |
PhylomeDBi | Q8BGV7 |
TreeFami | TF315649 |
Family and domain databases
InterProi | View protein in InterPro IPR000210, BTB/POZ_dom IPR011333, SKP1/BTB/POZ_sf IPR003131, T1-type_BTB |
Pfami | View protein in Pfam PF02214, BTB_2, 1 hit |
SMARTi | View protein in SMART SM00225, BTB, 1 hit |
SUPFAMi | SSF54695, SSF54695, 1 hit |
PROSITEi | View protein in PROSITE PS50097, BTB, 1 hit |
i Sequence
Sequence statusi: Complete.
Q8BGV7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSAEASGPAP AAAECLESPS PSSVEPGSPS YSLKPLTPNS KYVKLNVGGS
60 70 80 90 100
LHYTTLRTLT GQDTMLKAMF SGRVEVLTDA GGWVLIDRSG RHFGTILNYL
110 120 130 140 150
RDGSVPLPES ARELGELLGE ARYYLVQGLI EDCQLALQQK REKLSPLCLI
160 170 180 190 200
PTVTSPREEQ QLLASTSKPV VKLLHNRSNN KYSYTSTSDD NLLKNIELFD
210 220 230 240 250
KLALRFHGRL LFLKDVLGDE ICCWSFYGQG RKIAEVCCTS IVYATEKKQT
260 270 280 290 300
KVEFPEARIF EETLNILIYE NSRGPDLALL EATGGAAGGG GAGRGDDEEN
310 320
REHRVRRIHV RRHITHDERP HGQQIVFKD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF534881 mRNA Translation: AAO16177.1 AK075722 mRNA Translation: BAC35910.1 BC079607 mRNA Translation: AAH79607.1 |
CCDSi | CCDS21851.1 |
RefSeqi | NP_766335.1, NM_172747.2 |
Genome annotation databases
Ensembli | ENSMUST00000032924; ENSMUSP00000032924; ENSMUSG00000030685 |
GeneIDi | 233877 |
KEGGi | mmu:233877 |
UCSCi | uc009jtm.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF534881 mRNA Translation: AAO16177.1 AK075722 mRNA Translation: BAC35910.1 BC079607 mRNA Translation: AAH79607.1 |
CCDSi | CCDS21851.1 |
RefSeqi | NP_766335.1, NM_172747.2 |
3D structure databases
SMRi | Q8BGV7 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q8BGV7, 1 interactor |
STRINGi | 10090.ENSMUSP00000032924 |
PTM databases
PhosphoSitePlusi | Q8BGV7 |
Proteomic databases
EPDi | Q8BGV7 |
MaxQBi | Q8BGV7 |
PaxDbi | Q8BGV7 |
PRIDEi | Q8BGV7 |
Protocols and materials databases
Antibodypediai | 13396, 137 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000032924; ENSMUSP00000032924; ENSMUSG00000030685 |
GeneIDi | 233877 |
KEGGi | mmu:233877 |
UCSCi | uc009jtm.1, mouse |
Organism-specific databases
CTDi | 253980 |
MGIi | MGI:1923739, Kctd13 |
Phylogenomic databases
eggNOGi | KOG2716, Eukaryota |
GeneTreei | ENSGT00950000183143 |
HOGENOMi | CLU_060008_0_0_1 |
InParanoidi | Q8BGV7 |
OMAi | HYTTLQT |
OrthoDBi | 1306250at2759 |
PhylomeDBi | Q8BGV7 |
TreeFami | TF315649 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Miscellaneous databases
BioGRID-ORCSi | 233877, 1 hit in 17 CRISPR screens |
ChiTaRSi | Kctd13, mouse |
PROi | PR:Q8BGV7 |
RNActi | Q8BGV7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000030685, Expressed in cardiac ventricle and 213 other tissues |
Genevisiblei | Q8BGV7, MM |
Family and domain databases
InterProi | View protein in InterPro IPR000210, BTB/POZ_dom IPR011333, SKP1/BTB/POZ_sf IPR003131, T1-type_BTB |
Pfami | View protein in Pfam PF02214, BTB_2, 1 hit |
SMARTi | View protein in SMART SM00225, BTB, 1 hit |
SUPFAMi | SSF54695, SSF54695, 1 hit |
PROSITEi | View protein in PROSITE PS50097, BTB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BACD1_MOUSE | |
Accessioni | Q8BGV7Primary (citable) accession number: Q8BGV7 Secondary accession number(s): Q6AXE9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 31, 2004 |
Last sequence update: | March 1, 2003 | |
Last modified: | December 2, 2020 | |
This is version 125 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families