UniProtKB - Q8BGT9 (GLT12_MOUSE)
Protein
Polypeptide N-acetylgalactosaminyltransferase 12
Gene
Galnt12
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has activity toward non-glycosylated peptides such as Muc5AC, Muc1a and EA2, and no detectable activity with Muc2 and Muc7. Displays enzymatic activity toward the Gal-NAc-Muc5AC glycopeptide, but no detectable activity to mono-GalNAc-glycosylated Muc1a, Muc2, Muc7 and EA2. May play an important role in the initial step of mucin-type oligosaccharide biosynthesis in digestive organs (By similarity).By similarity
Catalytic activityi
- L-seryl-[protein] + UDP-N-acetyl-α-D-galactosamine = 3-O-[N-acetyl-α-D-galactosaminyl]-L-seryl-[protein] + H+ + UDPEC:2.4.1.41
- L-threonyl-[protein] + UDP-N-acetyl-α-D-galactosamine = 3-O-[N-acetyl-α-D-galactosaminyl]-L-threonyl-[protein] + H+ + UDPEC:2.4.1.41
Cofactori
Mn2+By similarity
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 171 | SubstrateBy similarity | 1 | |
Binding sitei | 200 | SubstrateBy similarity | 1 | |
Metal bindingi | 223 | ManganeseBy similarity | 1 | |
Metal bindingi | 225 | ManganeseBy similarity | 1 | |
Binding sitei | 330 | SubstrateBy similarity | 1 | |
Metal bindingi | 358 | ManganeseBy similarity | 1 | |
Binding sitei | 366 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- carbohydrate binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- polypeptide N-acetylgalactosaminyltransferase activity Source: UniProtKB-EC
GO - Biological processi
- protein glycosylation Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Ligand | Lectin, Manganese, Metal-binding |
Enzyme and pathway databases
Reactomei | R-MMU-913709, O-linked glycosylation of mucins |
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | CBM13, Carbohydrate-Binding Module Family 13 GT27, Glycosyltransferase Family 27 |
Names & Taxonomyi
Protein namesi | Recommended name: Polypeptide N-acetylgalactosaminyltransferase 12 (EC:2.4.1.41)Alternative name(s): Polypeptide GalNAc transferase 12 Short name: GalNAc-T12 Short name: pp-GaNTase 12 Protein-UDP acetylgalactosaminyltransferase 12 UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 12 |
Gene namesi | Name:Galnt12 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2444664, Galnt12 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane By similarity; Single-pass type II membrane protein By similarity
Golgi apparatus
- Golgi apparatus Source: GO_Central
- Golgi membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 19 | CytoplasmicSequence analysisAdd BLAST | 19 | |
Transmembranei | 20 – 37 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 18 | |
Topological domaini | 38 – 576 | LumenalSequence analysisAdd BLAST | 539 |
Keywords - Cellular componenti
Golgi apparatus, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000059129 | 1 – 576 | Polypeptide N-acetylgalactosaminyltransferase 12Add BLAST | 576 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 120 ↔ 353 | PROSITE-ProRule annotation | ||
Disulfide bondi | 344 ↔ 417 | PROSITE-ProRule annotation | ||
Disulfide bondi | 453 ↔ 474 | PROSITE-ProRule annotation | ||
Disulfide bondi | 501 ↔ 516 | PROSITE-ProRule annotation | ||
Disulfide bondi | 542 ↔ 561 | PROSITE-ProRule annotation |
Keywords - PTMi
Disulfide bondProteomic databases
EPDi | Q8BGT9 |
jPOSTi | Q8BGT9 |
MaxQBi | Q8BGT9 |
PaxDbi | Q8BGT9 |
PRIDEi | Q8BGT9 |
PTM databases
iPTMneti | Q8BGT9 |
PhosphoSitePlusi | Q8BGT9 |
SwissPalmi | Q8BGT9 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000039774, Expressed in molar tooth and 201 other tissues |
ExpressionAtlasi | Q8BGT9, baseline and differential |
Genevisiblei | Q8BGT9, MM |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000045721 |
Miscellaneous databases
RNActi | Q8BGT9, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 440 – 572 | Ricin B-type lectinPROSITE-ProRule annotationAdd BLAST | 133 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 130 – 239 | Catalytic subdomain AAdd BLAST | 110 | |
Regioni | 299 – 361 | Catalytic subdomain BAdd BLAST | 63 |
Domaini
There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.By similarity
The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.By similarity
Sequence similaritiesi
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3736, Eukaryota |
GeneTreei | ENSGT00940000157173 |
HOGENOMi | CLU_013477_0_3_1 |
InParanoidi | Q8BGT9 |
OMAi | QKWFFEE |
OrthoDBi | 606683at2759 |
PhylomeDBi | Q8BGT9 |
TreeFami | TF352660 |
Family and domain databases
CDDi | cd00161, RICIN, 1 hit |
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR001173, Glyco_trans_2-like IPR029044, Nucleotide-diphossugar_trans IPR035992, Ricin_B-like_lectins IPR000772, Ricin_B_lectin |
Pfami | View protein in Pfam PF00535, Glycos_transf_2, 1 hit PF00652, Ricin_B_lectin, 1 hit |
SMARTi | View protein in SMART SM00458, RICIN, 1 hit |
SUPFAMi | SSF50370, SSF50370, 1 hit SSF53448, SSF53448, 1 hit |
PROSITEi | View protein in PROSITE PS50231, RICIN_B_LECTIN, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q8BGT9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MWGRAVRRRC PRGLRRGREA LLALLALAGL GALLRARSRS GTVDPGPPRT
60 70 80 90 100
PLPGRHEPVL PRPPLPADAL GAHGEAVRLQ LQGEELRLQE ESVKQHQINI
110 120 130 140 150
YLSDRISLHR RLPERWNPLC REVKYDYDNL PKTSVVIAFY NEAWSTLLRT
160 170 180 190 200
VYSVLETSPD ILLEEVILVD DYSDREHLKE RLANELSQLP KVRLIRASRR
210 220 230 240 250
EGLVRARLLG ASAARGEVLT FLDCHCECHE GWLEPLLQRI HEKESAVVCP
260 270 280 290 300
VIDVIDWNTF EYLGNSGEPQ IGGFDWRLVF TWHVVPQRER QSMRSPIDVI
310 320 330 340 350
RSPTMAGGLF AVSKRYFDYL GSYDTGMEVW GGENLEFSFR IWQCGGTLET
360 370 380 390 400
HPCSHVGHVF PKQAPYSRSK ALANSVRAAE VWMDEFKELY YHRNPQARLE
410 420 430 440 450
PFGDVTERKK LRAKLQCKDF KWFLDTVYPE LHVPEDRPGF FGMLQNRGLR
460 470 480 490 500
GYCLDYNPPN ENHVEGHQVL LYLCHGMGQN QFFEYTTRKE IRYNTRQPEA
510 520 530 540 550
CITVEDGKDT LVMDLCRETV PENQEFILQE DGTLVHKHSR KCVEATEKVL
560 570
DNGFAPYLRD CTNSDNQRWF FKERMS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA2ANU2 | A2ANU2_MOUSE | Polypeptide N-acetylgalactosaminylt... | Galnt12 | 284 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK033638 mRNA Translation: BAC28401.1 AK042133 mRNA Translation: BAC31179.1 BC056425 mRNA Translation: AAH56425.1 |
CCDSi | CCDS18158.1 |
RefSeqi | NP_766281.1, NM_172693.3 |
Genome annotation databases
Ensembli | ENSMUST00000045041; ENSMUSP00000045721; ENSMUSG00000039774 |
GeneIDi | 230145 |
KEGGi | mmu:230145 |
UCSCi | uc008suk.1, mouse |
Similar proteinsi
Cross-referencesi
Web resourcesi
Functional Glycomics Gateway - GTase Polypeptide N-acetylgalactosaminyltransferase 12 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK033638 mRNA Translation: BAC28401.1 AK042133 mRNA Translation: BAC31179.1 BC056425 mRNA Translation: AAH56425.1 |
CCDSi | CCDS18158.1 |
RefSeqi | NP_766281.1, NM_172693.3 |
3D structure databases
SMRi | Q8BGT9 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000045721 |
Protein family/group databases
CAZyi | CBM13, Carbohydrate-Binding Module Family 13 GT27, Glycosyltransferase Family 27 |
PTM databases
iPTMneti | Q8BGT9 |
PhosphoSitePlusi | Q8BGT9 |
SwissPalmi | Q8BGT9 |
Proteomic databases
EPDi | Q8BGT9 |
jPOSTi | Q8BGT9 |
MaxQBi | Q8BGT9 |
PaxDbi | Q8BGT9 |
PRIDEi | Q8BGT9 |
Protocols and materials databases
Antibodypediai | 29005, 83 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000045041; ENSMUSP00000045721; ENSMUSG00000039774 |
GeneIDi | 230145 |
KEGGi | mmu:230145 |
UCSCi | uc008suk.1, mouse |
Organism-specific databases
CTDi | 79695 |
MGIi | MGI:2444664, Galnt12 |
Phylogenomic databases
eggNOGi | KOG3736, Eukaryota |
GeneTreei | ENSGT00940000157173 |
HOGENOMi | CLU_013477_0_3_1 |
InParanoidi | Q8BGT9 |
OMAi | QKWFFEE |
OrthoDBi | 606683at2759 |
PhylomeDBi | Q8BGT9 |
TreeFami | TF352660 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
Reactomei | R-MMU-913709, O-linked glycosylation of mucins |
Miscellaneous databases
BioGRID-ORCSi | 230145, 1 hit in 17 CRISPR screens |
ChiTaRSi | Galnt12, mouse |
PROi | PR:Q8BGT9 |
RNActi | Q8BGT9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000039774, Expressed in molar tooth and 201 other tissues |
ExpressionAtlasi | Q8BGT9, baseline and differential |
Genevisiblei | Q8BGT9, MM |
Family and domain databases
CDDi | cd00161, RICIN, 1 hit |
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR001173, Glyco_trans_2-like IPR029044, Nucleotide-diphossugar_trans IPR035992, Ricin_B-like_lectins IPR000772, Ricin_B_lectin |
Pfami | View protein in Pfam PF00535, Glycos_transf_2, 1 hit PF00652, Ricin_B_lectin, 1 hit |
SMARTi | View protein in SMART SM00458, RICIN, 1 hit |
SUPFAMi | SSF50370, SSF50370, 1 hit SSF53448, SSF53448, 1 hit |
PROSITEi | View protein in PROSITE PS50231, RICIN_B_LECTIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GLT12_MOUSE | |
Accessioni | Q8BGT9Primary (citable) accession number: Q8BGT9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 16, 2004 |
Last sequence update: | March 1, 2003 | |
Last modified: | December 2, 2020 | |
This is version 135 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families